scholarly journals Assessing the Individual and Combined Effects of QTL for Adult Plant Stripe Rust Resistance Derived from Cappelle-Desprez

Agronomy ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 154 ◽  
Author(s):  
Gerrie Maree ◽  
Renée Prins ◽  
Lesley Boyd ◽  
Howard Castelyn ◽  
Cornelia Bender ◽  
...  
Keyword(s):  
South African ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Recombinant Inbred Lines ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Breeding Programs ◽  
Field Assessment ◽  
The Individual

The release of commercial wheat cultivars resistant to stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), remains one of the primary objectives in many breeding programs. Previous studies of adult plant resistance derived from the winter wheat cultivar Cappelle-Desprez identified the quantitative trait loci (QTL) QYr.ufs-2A, QYr.ufs-2D, QYr.ufs-5B and QYr.ufs-6D to affect stripe rust under South African conditions. Phenotypic field assessment, fluorescence microscopy and molecular analysis were used to characterise recombinant inbred lines differing in number and combinations of these QTL. Besides the confirmation of enhanced resistance through co-occurring resistance loci, varying levels of defence, conditioned by different QTL combinations were observed. Carriers of QYr.ufs-2A or QYr.ufs-2D, accompanied by at least one other QTL, exhibited higher resistance levels than lines with a single QTL. The knowledge gained in this study will help wheat breeders to develop cultivars with more diverse combinations and potentially more durable sources of stripe rust resistance.

scholarly journals Barley Stripe Rust Resistance QTL: Development and Validation of SNP Markers for Resistance to Puccinia striiformis f. sp. hordei

2016 ◽  
Vol 106 (11) ◽  
pp. 1344-1351 ◽  
Author(s):  
K. Esvelt Klos ◽  
T. Gordon ◽  
P. Bregitzer ◽  
P. Hayes ◽  
X. M. Chen ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Recombinant Inbred Lines ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Resistance Qtl ◽  
Barley Stripe Rust

Quantitative trait loci (QTL) for barley stripe rust resistance were mapped in recombinant inbred lines (RIL) from a ‘Lenetah’ × ‘Grannelose Zweizeilige’ (GZ) cross. GZ is known for a major seedling resistance QTL on chromosome 4H but linked markers suitable for marker-assisted selection have not been developed. This study identified the 4H QTL (log of the likelihood [LOD] = 15.94 at 97.19 centimorgans [cM]), and additional QTL on chromosomes 4H and 6H (LOD = 5.39 at 72.7 cM and 4.24 at 34.46 cM, respectively). A QTL on chromosome 7H (LOD = 2.04 at 81.07 cM) was suggested. All resistance alleles were derived from GZ. Evaluations of adult plant response in Corvallis, OR in 2013 and 2015 provided evidence of QTL at the same positions. However, the minor QTL on 4H was not statistically significant in either location/year, while the 7H QTL was significant in both. The single-nucleotide polymorphism markers flanking the resistance QTL were validated in RIL from a ‘95SR316A’ × GZ cross for their ability to predict seedling resistance. In 95SR316A × GZ, 91 to 92% of RIL with GZ alleles at the major 4H QTL and at least one other were resistant to moderate in reaction. In these populations, at least two QTL were required to transfer the barley stripe rust resistance from GZ.

scholarly journals Development and Validation of KASP-SNP Markers for QTL Underlying Resistance to Stripe Rust in Common Wheat Cultivar P10057

Plant Disease ◽  
2017 ◽  
Vol 101 (12) ◽  
pp. 2079-2087 ◽  
Author(s):  
Jianhui Wu ◽  
Qilin Wang ◽  
Zhensheng Kang ◽  
Shengjie Liu ◽  
Haiyang Li ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Cultivar ◽  
Infection Type ◽  
Recombinant Inbred Lines ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Kasp Markers

Stripe rust (Puccinia striiformis f. sp. tritici) is among the most important diseases of wheat (Triticum aestivum L.) globally. Utilization of adult plant resistance (APR) constitutes a key tool for maintaining protection against this disease. The CIMMYT wheat cultivar P10057 displayed a high level of APR to stripe rust in germplasm evaluation in field environments. To clarify the genetic basis and identify quantitative trait loci (QTLs) involved in stripe rust resistance in P10057, three wheat populations were used: 150 F5:6 recombinant inbred lines (RILs) derived from the cross Mingxian 169 × P10057, and 161 and 140 F2:3 lines from Avocet S × P10057 and Zhengmai 9023 × P10057, respectively. These three populations were evaluated for infection type (IT) and disease severity (DS) in Shaanxi, Gansu, and Sichuan during the 2014–15 and 2015–16 cropping seasons. Genotyping was performed with Kompetitive Allelic Specific PCR (KASP) and simple sequence repeat (SSR) markers linked to the resistance loci. Using QTL analysis, two genomic regions associated with resistance were found on chromosome arms 2BS and 3BS, respectively. These two stable QTLs, designated Qyrlov.nwafu-2BS and Qyrlov.nwafu-3BS, were detected across all environments and explained average 22.6 to 31.6% and 21.3 to 32.3% of stripe rust severity phenotypic variation, respectively. Qyrlov.nwafu-2BS may be the resistance allele derived from CIMMYT germplasm and Qyrlov.nwafu-3BS likely corresponds to the locus Sr2/Lr27/Yr30/Pbc. The KASP markers IWA5377, IWA2674, and IWA5830 linked to QYrlov.nwafu-2BS and IWB57990 and IWB6491 linked to Qyrlov.nwafu-3BS were reliable for marker-assisted selection (MAS) in the Zhengmai 9023 × P10057 population. These QTLs with KASP markers are expected to contribute in developing wheat cultivars with improved stripe rust resistance.

scholarly journals QTL mapping of adult plant resistance to stripe rust in the Fundulea 900 × Thatcher RIL population

2021 ◽  
Vol 57 (No. 1) ◽  
pp. 1-8
Author(s):  
Xiaocui Yan ◽  
Huimin Zheng ◽  
Peipei Zhang ◽  
Gebrewahid Takele Weldu ◽  
Zaifeng Li ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Recombinant Inbred Lines ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Phenotypic Variance ◽  
Wheat Stripe Rust ◽  
Ril Population

Wheat stripe rust, caused by Puccinia striiformis Westend. f.sp. tritici Eriks (Pst), is one of the most important diseases of bread wheat worldwide. Breeding resistant wheat cultivars is the most economical, effective and environmentally friendly way for controlling wheat stripe rust in China. The Romanian wheat line Fundulea 900 showed good resistance to wheat stripe rust at the adult stage. The present study aimed to map the quantitative trait loci (QTLs) for stripe rust resistance in 176 F<sub>2:6</sub> recombinant inbred lines (RIL) derived from the cross of Fundulea 900 × Thatcher. The RIL population was phenotyped for stripe rust (YR) severity at Mianyang in the Sichuan province and Baoding in the Hebei province in the 2016/2017 and 2017/2018 cropping seasons. SSR markers combined with a preferred screened group (PSG) analysis were used to identify the QTLs for stripe rust in the population. Three QTLs for stripe rust resistance were mapped on chromosomes 1AL, 7BL and 7DS, respectively. All three QTLs originated from Fundulea 900 and were detected in all the environments. The QTL on 7DS was provided by the known resistance gene Yr18/Lr34. The two QTLs on chromosomes 1AL and 7BL were explained by 9.2 to 21.5% and 5.1 to 10.1% of the phenotypic variance, respectively and might be new QTLs. The QTLs identified in the study and their closely linked markers can be used for marker-assisted selection (MAS) in wheat breeding programmes.

scholarly journals Quantitative Trait Loci Mapping of Adult Plant and Seedling Resistance to Stripe Rust (Puccinia striiformis Westend.) in a Multiparent Advanced Generation Intercross Wheat Population

2021 ◽  
Vol 12 ◽  
Author(s):  
Sandra Rollar ◽  
Manuel Geyer ◽  
Lorenz Hartl ◽  
Volker Mohler ◽  
Frank Ordon ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Wheat Population ◽  
Advanced Generation

Stripe rust caused by the biotrophic fungus Puccinia striiformis Westend. is one of the most important diseases of wheat worldwide, causing high yield and quality losses. Growing resistant cultivars is the most efficient way to control stripe rust, both economically and ecologically. Known resistance genes are already present in numerous cultivars worldwide. However, their effectiveness is limited to certain races within a rust population and the emergence of stripe rust races being virulent against common resistance genes forces the demand for new sources of resistance. Multiparent advanced generation intercross (MAGIC) populations have proven to be a powerful tool to carry out genetic studies on economically important traits. In this study, interval mapping was performed to map quantitative trait loci (QTL) for stripe rust resistance in the Bavarian MAGIC wheat population, comprising 394 F6 : 8 recombinant inbred lines (RILs). Phenotypic evaluation of the RILs was carried out for adult plant resistance in field trials at three locations across three years and for seedling resistance in a growth chamber. In total, 21 QTL for stripe rust resistance corresponding to 13 distinct chromosomal regions were detected, of which two may represent putatively new QTL located on wheat chromosomes 3D and 7D.

scholarly journals Genome-wide Mapping for Stripe Rust Resistance Loci in Common Wheat Cultivar Qinnong 142

Plant Disease ◽  
2019 ◽  
Vol 103 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Qingdong Zeng ◽  
Jianhui Wu ◽  
Shengjie Liu ◽  
Xianming Chen ◽  
Fengping Yuan ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Cultivar ◽  
Infection Type ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars

Stripe rust caused by Puccinia striiformis f. sp. tritici threatens worldwide wheat production. Growing resistant cultivars is the best way to control this disease. Chinese wheat cultivar Qinnong 142 (QN142) has a high level of adult-plant resistance to stripe rust. To identify quantitative trait loci (QTLs) related to stripe rust resistance, we developed a recombinant inbred line (RIL) population from a cross between QN142 and susceptible cultivar Avocet S. The parents and 165 F6 RILs were evaluated in terms of their stripe rust infection type and disease severity in replicated field tests with six site-year environments. The parents and RILs were genotyped with single-nucleotide polymorphism (SNP) markers. Four stable QTLs were identified in QN142 and mapped to chromosome arms 1BL, 2AL, 2BL, and 6BS. The 1BL QTL was probably the known resistance gene Yr29, the 2BL QTL was in a resistance gene-rich region, and the 2AL and 6BS QTLs might be new. Kompetitive allele specific polymerase chain reaction markers developed from the SNP markers flanking these QTLs were highly polymorphic in a panel of 150 wheat cultivars and breeding lines. These markers could be used in marker-assisted selection for incorporating the stripe rust resistance QTL into new wheat cultivars.

scholarly journals Identification of Candidate Genes and Genomic Regions Associated with Adult Plant Resistance to Stripe Rust in Spring Wheat

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2585
Author(s):  
Amira M. I. Mourad ◽  
Mohamed A. Abou-Zeid ◽  
Shamseldeen Eltaher ◽  
P. Stephen Baenziger ◽  
Andreas Börner
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Yield ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
P Value ◽  
Dart Markers ◽  
Single Marker Analysis ◽  
Gene Models

Wheat stripe rust (caused by Puccinia striiformis f. sp. tritici) is a major disease that damages wheat plants and affects wheat yield all over the world. In recent years, stripe rust became a major problem that affects wheat yield in Egypt. New races appeared and caused breakdowns in the resistant genotypes. To improve resistance in the Egyptian genotypes, new sources of resistance are urgently needed. In the recent research, a set of 95 wheat genotypes collected from 19 countries, including Egypt, were evaluated for their resistance against the Egyptian race(s) of stripe rust under field conditions in the two growing seasons 2018/2019 and 2019/2020. A high genetic variation was found among the tested genotypes. Single marker analysis was conducted using a subset of 71 genotypes and 424 diversity array technology (DArT) markers, well distributed across the genome. Out of the tested markers, 13 stable markers were identified that were significantly associated with resistance in both years (p-value ≤ 0.05). By using the sequence of the DArT markers, the chromosomal position of the significant DArT markers was detected, and nearby gene models were identified. Two markers on chromosomes 5A and 5B were found to be located within gene models functionally annotated with disease resistance in plants. These two markers could be used in marker-assisted selection for stripe rust resistance under Egyptian conditions. Two German genotypes were carrying the targeted allele of all the significant DArT markers associated with stripe rust resistance and could be used to improve resistance under Egyptian conditions.

Genetics of stripe rust resistance in 'Karamu' wheat (Triticum aestivum L.)

2005 ◽  
Vol 56 (6) ◽  
pp. 619 ◽  
Author(s):  
M. Imtiaz ◽  
M. G. Cromey ◽  
J. G. Hampton ◽  
F. C. Ogbonnaya
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Puccinia Striiformis ◽  
Triticum Aestivum L ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Dh Lines ◽  
Disease Pressure

The New Zealand wheat (Triticum aestivum L.) cv. Karamu (same parentage as the US cv. Anza) was originally believed to carry gene Yr18, which provides adult plant resistance to stripe rust (Puccinia striiformis f. sp. tritici), in addition to the seedling resistance gene YrA. Following the detection of virulence to the stripe rust resistance gene YrA in 1995, much of the resistance of Karamu was eroded and the cultivar suffered from occasional severe stripe rust outbreaks. This meant that either one or more new races of Puccinia striiformis f. sp. tritici with virulence to Yr18 had developed, or that Yr18 conferred inadequate resistance under high disease pressure. Karamu was crossed with cv. Otane, which carries Yr18, and 140 double haploid (DH) lines obtained from the F1 progeny were evaluated for seedling and adult plant resistance under greenhouse and field conditions. Evaluation of F1 plants against stripe rust pathotype 106E139A+ revealed that the resistance was recessive and that none of the resistance genes present was effective at the seedling stage. Segregation in the DH lines at the adult plant stage indicated that the resistance measured through infection type in both the greenhouse and the field was based on 3 genes, 1 from Karamu and 2 from Otane. However, the resistance gene from Karamu did not contribute towards resistance measured through final disease severity, but acted additively with genes from Otane in providing slow-rusting resistance as expressed by lower values for area under the disease progress curve. It was concluded that Karamu does not have gene Yr18, but rather possesses a recessive minor gene, which explains its adult plant susceptibility under high disease pressure. However, this Karamu gene did interact with Otane resistance genes to provide increased resistance.

Genetics of stripe rust resistance in two barley (Hordeum vulgare L.) genotypes

2019 ◽  
Vol 79 (02) ◽  
Author(s):  
Vishnu Kumar ◽  
S. C. Bhardwaj ◽  
A. S. Kharub ◽  
G. P. Singh
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Dominant Gene ◽  
Malting Quality ◽  
Stripe Rust Resistance ◽  
Yield Reduction ◽  
Adult Plant ◽  
Hordeum Vulgare L ◽  
F2 Population

Stripe rust inflicted by the pathogen Puccinia striiformis Westend. f. sp. hordei Eriks can cause marked grain yield reduction and deterioration of the malting quality in barley. Two barley genotypes, DWRB137 (DWR28/DWRUB64) and DWRB143 (DWRB73/DWR83) were identified as resistant (R) for three stripe rust races viz., 57 (0S0), M (1S0) and Q (5S0) at seedling and adult plant stages. These genotypes were crossed with two stripe rust susceptible (S) barley cultivars, RD2035 and Lakhan to study mode of inheritance of stripe rust resistance. All the F1 plants showed resistance against stripe rust race, 57 (0S0) indicating that the resistance (R) is dominant. The F2 generations derived from the crosses namely, Lakhan/DWRB137, Lakhan/DWRB143, RD2035/DWRB137 and RD2035/DWRB143 were tested with inoculum of race, 57 (0S0) under artificially inoculated conditions. The observed frequency of segregants in each F2 generation fit well in a theoretical ratio of 3(R):1(S) (χ 2(T) less than 0.01) indicating that the resistance against the tested race is controlled by a single dominant gene in the genotypes, DWRB137 and DWRB143. To validate the F2 hypothesis, the F3 progenies were also tested under above described conditions and followed discrete segregation of 1 (R): 2 (Segregating): 1(S) ratio in all the four crosses. Test of allelism was also conducted to establish the identity of resistance gene(s) present in the resistant genotypes. The F2 population derived from DWRB137/DWRB143 (R × R) cross fit to 15(R):1(S) ratio showing that the two genotypes had different resistance genes.

scholarly journals Effective Resistance to Wheat Stripe Rust in a Region with High Disease Pressure

Plant Disease ◽  
2014 ◽  
Vol 98 (7) ◽  
pp. 891-897 ◽  
Author(s):  
B. Bai ◽  
J. Y. Du ◽  
Q. L. Lu ◽  
C. Y. He ◽  
L. J. Zhang ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Hot Spot ◽  
Infection Type ◽  
Durable Resistance ◽  
Gansu Province ◽  
Stripe Rust Resistance ◽  
Adult Plant

Stripe rust is a major fungal disease of wheat. It frequently becomes epidemic in southeastern Gansu province, a stripe rust hot spot in China. Evaluations of wheat germplasm response are crucial for developing cultivars to control the disease. In total, 57 wheat cultivars and lines from Europe and other countries, comprising 36 cultivars with documented stripe rust resistance genes and 21 with unknown genes, were tested annually with multiple races of Puccinia striiformis f. sp. tritici in the field at Tianshui in Gansu province from 1993 to 2013. Seven wheat lines were highly resistant, with infection type (IT) 0 during the entire period; 16 were moderately resistant (IT 0;-2); and 26 were moderately susceptible (IT 0;-4), with low maximum disease severity compared with the susceptible control Huixianhong. ‘Strampelli’ and ‘Libellula’, with three and five quantitative trait loci, respectively, for stripe rust resistance have displayed durable resistance in this region for four decades. Ten cultivars, including ‘Lantian 15’, ‘Lantian 26’, and ‘Lantian 31’, with stripe rust resistance derived from European lines, were developed in our breeding program and have made a significant impact on controlling stripe rust in southeastern Gansu. Breeding resistant cultivars with multiple adult-plant resistance genes seems to be a promising strategy in wheat breeding for managing stripe rust in this region and other hot spots.

Stripe rust resistance among western Canadian spring wheat and triticale varieties

2012 ◽  
Vol 92 (4) ◽  
pp. 713-722 ◽  
Author(s):  
H. Randhawa ◽  
B. J. Puchalski ◽  
M. Frick ◽  
A. Goyal ◽  
T. Despins ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Spring Wheat ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Western Canada ◽  
Resistant Varieties ◽  
Plant Resistance Gene ◽  
Moderately Resistant

Randhawa, H., Puchalski, B. J., Frick, M., Goyal, A., Despins, T., Graf, R. J., Laroche, A. and Gaudet, D. A. 2012. Stripe rust resistance among western Canadian spring wheat and triticale varieties. Can. J. Plant Sci. 92: 713–722. Stripe rust (Puccinia striiformis Westend.) is an important pathogen of wheat in western Canada and worldwide. One hundred and four spring wheat and triticale varieties and cultivars were evaluated for resistance to stripe rust in nurseries at Lethbridge and Creston, BC, during 2009 and 2010. In the Canada Prairie Spring Red (CPSR) wheat class, newer varieties were more resistant compared with many of the older varieties. Among the white Canada Prairie Spring White (CPSW) wheats, Vista was moderately resistant, whereas Snowhite475 and Snowhite476 were susceptible. Little useful resistance was observed within the Canada Western Hard White Spring (CWHWS) class. Sixty percent of the Canada Western Red Spring (CWRS) wheats, including the currently popular varieties Lillian, Harvest and Kane, were resistant. Susceptible CWRS varieties that are extensively seeded in western Canada include AC Barrie, Superb and McKenzie, but also include the recently registered CDC Kernen and Vesper. The varieties were tested for the presence of the stripe rust genes Yr10, Yr17, Yr18 and Yr36 using molecular markers. Much of the stripe rust resistance, particularly in the CWRS, Canada Western Extra Strong (CWES), and CPSR wheat classes was attributed to the presence of adult plant resistance gene Yr18. Yr17 and Yr36 were also detected among CWRS and CWES varieties. However, the absence of markers for known genes in several resistant varieties indicated that uncharacterized genes for stripe rust occur among hexaploid wheats. Durum wheat and triticale varieties were universally resistant with the absence of tested markers. Therefore, there appear to be numerous sources of stripe rust resistance, both characterized and uncharacterized, among western Canadian spring wheat and triticale varieties.

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