Multi-Pathotype Testing of Selected Kenyan Wheat Germplasm and Watkin Landraces for Resistance to Wheat Stripe Rust (Puccinia striiformis f. sp tritici) Races

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the key diseases of economic importance in wheat worldwide. Host resistance, which follows the gene-for-gene hypothesis between the host and pathogen, has been used in wheat lines to resolve resistance specificities and postulate resistant genes. The objective of this study was to elucidate stripe rust resistance in a collection of Kenyan wheat lines and Watkin landraces to identify new sources of stripe rust (Yr) resistance. In this study, the resistance in twenty wheat lines was determined by comparing their infection type with those of twenty differential lines using isolates representing twelve Puccinia striiformis races from Kenya, Denmark, U.K., Sweden, and Eritrea at the seedling stage. Among the twenty wheat lines, none was resistant to all the twelve Pst races and isolate DK02d/12 (“Kranich” race) was virulent on all the genotypes except wheat genotype “Kenya Tai.” This genotype (“Kenya Tai”) had the highest resistance as it was resistant to all the twelve stripe rust races used in this study. From this study, the introduction and utilization of wheat genotypes with adult plant resistant (APR) stripe rust genes, such as Yr15, are important in breeding wheat genotypes with effective resistance to wheat stripe rust in Kenya and worldwide.

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Genome-wide Mapping for Stripe Rust Resistance Loci in Common Wheat Cultivar Qinnong 142

Plant Disease ◽

10.1094/pdis-05-18-0846-re ◽

2019 ◽

Vol 103 (3) ◽

pp. 439-447 ◽

Cited By ~ 4

Author(s):

Qingdong Zeng ◽

Jianhui Wu ◽

Shengjie Liu ◽

Xianming Chen ◽

Fengping Yuan ◽

...

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Wheat Cultivar ◽

Infection Type ◽

Snp Markers ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Wheat Cultivars

Stripe rust caused by Puccinia striiformis f. sp. tritici threatens worldwide wheat production. Growing resistant cultivars is the best way to control this disease. Chinese wheat cultivar Qinnong 142 (QN142) has a high level of adult-plant resistance to stripe rust. To identify quantitative trait loci (QTLs) related to stripe rust resistance, we developed a recombinant inbred line (RIL) population from a cross between QN142 and susceptible cultivar Avocet S. The parents and 165 F6 RILs were evaluated in terms of their stripe rust infection type and disease severity in replicated field tests with six site-year environments. The parents and RILs were genotyped with single-nucleotide polymorphism (SNP) markers. Four stable QTLs were identified in QN142 and mapped to chromosome arms 1BL, 2AL, 2BL, and 6BS. The 1BL QTL was probably the known resistance gene Yr29, the 2BL QTL was in a resistance gene-rich region, and the 2AL and 6BS QTLs might be new. Kompetitive allele specific polymerase chain reaction markers developed from the SNP markers flanking these QTLs were highly polymorphic in a panel of 150 wheat cultivars and breeding lines. These markers could be used in marker-assisted selection for incorporating the stripe rust resistance QTL into new wheat cultivars.

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Effective Resistance to Wheat Stripe Rust in a Region with High Disease Pressure

Plant Disease ◽

10.1094/pdis-09-13-0909-re ◽

2014 ◽

Vol 98 (7) ◽

pp. 891-897 ◽

Cited By ~ 6

Author(s):

B. Bai ◽

J. Y. Du ◽

Q. L. Lu ◽

C. Y. He ◽

L. J. Zhang ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Hot Spot ◽

Infection Type ◽

Durable Resistance ◽

Gansu Province ◽

Stripe Rust Resistance ◽

Adult Plant

Stripe rust is a major fungal disease of wheat. It frequently becomes epidemic in southeastern Gansu province, a stripe rust hot spot in China. Evaluations of wheat germplasm response are crucial for developing cultivars to control the disease. In total, 57 wheat cultivars and lines from Europe and other countries, comprising 36 cultivars with documented stripe rust resistance genes and 21 with unknown genes, were tested annually with multiple races of Puccinia striiformis f. sp. tritici in the field at Tianshui in Gansu province from 1993 to 2013. Seven wheat lines were highly resistant, with infection type (IT) 0 during the entire period; 16 were moderately resistant (IT 0;-2); and 26 were moderately susceptible (IT 0;-4), with low maximum disease severity compared with the susceptible control Huixianhong. ‘Strampelli’ and ‘Libellula’, with three and five quantitative trait loci, respectively, for stripe rust resistance have displayed durable resistance in this region for four decades. Ten cultivars, including ‘Lantian 15’, ‘Lantian 26’, and ‘Lantian 31’, with stripe rust resistance derived from European lines, were developed in our breeding program and have made a significant impact on controlling stripe rust in southeastern Gansu. Breeding resistant cultivars with multiple adult-plant resistance genes seems to be a promising strategy in wheat breeding for managing stripe rust in this region and other hot spots.

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Genetic Relationship of Stripe Rust Resistance Genes Yr34 and Yr48 in Wheat and Identification of Linked KASP Markers

Plant Disease ◽

10.1094/pdis-08-17-1144-re ◽

2018 ◽

Vol 102 (2) ◽

pp. 413-420 ◽

Cited By ~ 15

Author(s):

N. Qureshi ◽

H. S. Bariana ◽

P. Zhang ◽

R. McIntosh ◽

U. K. Bansal ◽

...

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Rust Resistance Gene ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Wheat Genotypes ◽

Stripe Rust Resistance Gene ◽

Australian Continent

The Australian continent was free from wheat stripe rust caused by Puccinia striiformis f. sp. tritici until exotic incursions occurred in 1979 and 2002. The 2002 incursion enabled the identification of a new stripe rust resistance gene (Yr34) in the advanced breeding line WAWHT2046. In this study, we developed and validated markers closely linked with Yr34, which is located in the distal region in the long arm of chromosome 5A. Four kompetitive allele-specific polymerase chain reaction (KASP) and three sequence-tagged site (STS) markers derived from the International Wheat Genome Sequencing Consortium RefSeq v1.0 scaffold-77836 cosegregated with Yr34. Markers sun711, sun712, sun725, sunKASP_109, and sunKASP_112 were shown to be suitable for marker-assisted selection in a validation panel of 71 Australian spring wheat genotypes, with the exception of cultivar Orion that carried the Yr34-linked alleles for sunKASP_109 and sunKASP_112. Markers previously reported to be linked with adult plant stripe rust resistance gene Yr48 also cosegregated with Yr34. Wheat genotypes carrying Yr34 and Yr48 produced identical haplotypes for the Yr34-linked markers identified in this study and those previously reported to be linked with Yr48. Phenotypic testing of genotypes carrying Yr34 and Yr48 showed that both genes conferred similar seedling responses to pre-2002 and post-2002 P. striiformis f. sp. tritici pathotypes. Further testing of 600 F2 plants from a cross between WAWHT2046 and RIL143 (Yr48) with P. striiformis f. sp. tritici pathotype 134 E16A+Yr17+Yr27+ failed to reveal any susceptible segregants. Our results strongly suggest that Yr34 and Yr48 are the same gene, and that Yr48 should be considered a synonym of Yr34.

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Development and Validation of KASP-SNP Markers for QTL Underlying Resistance to Stripe Rust in Common Wheat Cultivar P10057

Plant Disease ◽

10.1094/pdis-04-17-0468-re ◽

2017 ◽

Vol 101 (12) ◽

pp. 2079-2087 ◽

Cited By ~ 12

Author(s):

Jianhui Wu ◽

Qilin Wang ◽

Zhensheng Kang ◽

Shengjie Liu ◽

Haiyang Li ◽

...

Keyword(s):

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Wheat Cultivar ◽

Infection Type ◽

Recombinant Inbred Lines ◽

Snp Markers ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Kasp Markers

Stripe rust (Puccinia striiformis f. sp. tritici) is among the most important diseases of wheat (Triticum aestivum L.) globally. Utilization of adult plant resistance (APR) constitutes a key tool for maintaining protection against this disease. The CIMMYT wheat cultivar P10057 displayed a high level of APR to stripe rust in germplasm evaluation in field environments. To clarify the genetic basis and identify quantitative trait loci (QTLs) involved in stripe rust resistance in P10057, three wheat populations were used: 150 F5:6 recombinant inbred lines (RILs) derived from the cross Mingxian 169 × P10057, and 161 and 140 F2:3 lines from Avocet S × P10057 and Zhengmai 9023 × P10057, respectively. These three populations were evaluated for infection type (IT) and disease severity (DS) in Shaanxi, Gansu, and Sichuan during the 2014–15 and 2015–16 cropping seasons. Genotyping was performed with Kompetitive Allelic Specific PCR (KASP) and simple sequence repeat (SSR) markers linked to the resistance loci. Using QTL analysis, two genomic regions associated with resistance were found on chromosome arms 2BS and 3BS, respectively. These two stable QTLs, designated Qyrlov.nwafu-2BS and Qyrlov.nwafu-3BS, were detected across all environments and explained average 22.6 to 31.6% and 21.3 to 32.3% of stripe rust severity phenotypic variation, respectively. Qyrlov.nwafu-2BS may be the resistance allele derived from CIMMYT germplasm and Qyrlov.nwafu-3BS likely corresponds to the locus Sr2/Lr27/Yr30/Pbc. The KASP markers IWA5377, IWA2674, and IWA5830 linked to QYrlov.nwafu-2BS and IWB57990 and IWB6491 linked to Qyrlov.nwafu-3BS were reliable for marker-assisted selection (MAS) in the Zhengmai 9023 × P10057 population. These QTLs with KASP markers are expected to contribute in developing wheat cultivars with improved stripe rust resistance.

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Characterization of Stripe Rust Resistance in Wheat Lines with Resistance Gene Yr17 and Implications for Evaluating Resistance and Virulence

Phytopathology ◽

10.1094/phyto-11-14-0304-r ◽

2015 ◽

Vol 105 (8) ◽

pp. 1123-1130 ◽

Cited By ~ 22

Author(s):

Eugene A. Milus ◽

Kevin D. Lee ◽

Gina Brown-Guedira

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Puccinia Striiformis ◽

Infection Type ◽

Stripe Rust Resistance ◽

Wheat Seedlings ◽

South Central ◽

Infection Types ◽

Wheat Lines ◽

Adult Plants

Stripe rust, caused by Puccinia striiformis f. sp. tritici, has been the most important foliar wheat disease in south central United States since 2000 when a new strain of the pathogen emerged. The resistance gene Yr17 was used by many breeding programs to develop resistant cultivars. Although Yr17 was classified as a seedling (all-stage) resistance gene conferring a low infection type, seedlings with Yr17 frequently had intermediate to high infection types when inoculated with isolates that caused little or no disease on adult plants of the same wheat lines. The objectives of this study were to determine how to best evaluate Yr17 resistance in wheat lines and to determine which factors made seedling tests involving Yr17 so variable. Stripe rust reactions on wheat seedlings with Yr17 were influenced by temperature, wheat genotype, pathogen isolate, and the leaf (first or second) used to assess the seedling reaction. The most critical factors for accurately evaluating Yr17 reactions at the seedling stage were to avoid night temperatures below 12°C, to use the first leaf to assess the seedling reaction, to use multiple differentials with Yr17 and known avirulent, partially virulent and virulent isolates as controls, and to recognize that intermediate infection types likely represent a level of partial virulence in the pathogen that is insufficient to cause disease on adult plants in the field.

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Genome-Wide Association Mapping for Stripe Rust Resistance in Pakistani Spring Wheat Genotypes

Plants ◽

10.3390/plants9091056 ◽

2020 ◽

Vol 9 (9) ◽

pp. 1056 ◽

Cited By ~ 1

Author(s):

Madiha Habib ◽

Faisal Saeed Awan ◽

Bushra Sadia ◽

Muhammad Anjum Zia

Keyword(s):

Stripe Rust ◽

Spring Wheat ◽

Puccinia Striiformis ◽

Genetic Resistance ◽

Snp Markers ◽

Yield Potential ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Wheat Genotypes ◽

Research Findings

Stripe rust caused by the pathogen Puccinia striiformis f. sp. tritici (Pst) is a major threat for wheat, resulting in low yield and grain quality loss in many countries. Genetic resistance is a prevalent method to combat the disease. Mapping the resistant loci and their association with traits is highly exploited in this era. A panel of 465 Pakistani spring wheat genotypes were evaluated for their phenotypic response to stripe rust at the seedling and adult plant stages. A total of 765 single nucleotide polymorphism (SNP) markers were applied on 465 wheat genotypes to evaluate their stripe rust response against nine races during the seedling test and in three locations for the field test. Currently, twenty SNPs dispersed on twelve chromosomal regions (1A, 1B, 1D, 2A, 2B, 4A, 4B, 5B, 6A, 6B, 6D and 7B) have been identified that were associated with rust race-specific resistance at the seedling stage. Thirty SNPs dispersed on eighteen chromosomal regions (1A, 1B, 1D, 2A, 2B, 2D, 3A, 3B, 3D, 4B, 5A, 5B, 6A, 6B, 6D, 7A, 7B and 7D) are associated with adult plant resistance. SNP loci IWB3662 was linked with all three Pakistani races, and likewise IWA2344 and IWA4096 were found to be linked with three different USA races. The present research findings can be applied by wheat breeders to increase their resistant capability and yield potential of their cultivars, through marker-assisted selection.

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QTL mapping of adult plant resistance to stripe rust in the Fundulea 900 × Thatcher RIL population

Czech Journal of Genetics and Plant Breeding ◽

10.17221/71/2020-cjgpb ◽

2021 ◽

Vol 57 (No. 1) ◽

pp. 1-8

Author(s):

Xiaocui Yan ◽

Huimin Zheng ◽

Peipei Zhang ◽

Gebrewahid Takele Weldu ◽

Zaifeng Li ◽

...

Keyword(s):

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Recombinant Inbred Lines ◽

Wheat Breeding ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Phenotypic Variance ◽

Wheat Stripe Rust ◽

Ril Population

Wheat stripe rust, caused by Puccinia striiformis Westend. f.sp. tritici Eriks (Pst), is one of the most important diseases of bread wheat worldwide. Breeding resistant wheat cultivars is the most economical, effective and environmentally friendly way for controlling wheat stripe rust in China. The Romanian wheat line Fundulea 900 showed good resistance to wheat stripe rust at the adult stage. The present study aimed to map the quantitative trait loci (QTLs) for stripe rust resistance in 176 F<sub>2:6</sub> recombinant inbred lines (RIL) derived from the cross of Fundulea 900 × Thatcher. The RIL population was phenotyped for stripe rust (YR) severity at Mianyang in the Sichuan province and Baoding in the Hebei province in the 2016/2017 and 2017/2018 cropping seasons. SSR markers combined with a preferred screened group (PSG) analysis were used to identify the QTLs for stripe rust in the population. Three QTLs for stripe rust resistance were mapped on chromosomes 1AL, 7BL and 7DS, respectively. All three QTLs originated from Fundulea 900 and were detected in all the environments. The QTL on 7DS was provided by the known resistance gene Yr18/Lr34. The two QTLs on chromosomes 1AL and 7BL were explained by 9.2 to 21.5% and 5.1 to 10.1% of the phenotypic variance, respectively and might be new QTLs. The QTLs identified in the study and their closely linked markers can be used for marker-assisted selection (MAS) in wheat breeding programmes.

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Inheritance and Molecular Mapping of Barley Genes Conferring Resistance to Wheat Stripe Rust

Phytopathology ◽

10.1094/phyto-95-0884 ◽

2005 ◽

Vol 95 (8) ◽

pp. 884-889 ◽

Cited By ~ 22

Author(s):

Vihanga Pahalawatta ◽

Xianming Chen

Keyword(s):

Resistance Gene ◽

Microsatellite Marker ◽

Stripe Rust ◽

Molecular Mapping ◽

Puccinia Striiformis ◽

Infection Type ◽

Dominant Gene ◽

Wheat Stripe Rust ◽

Rust Pathogen ◽

Type Data

Most barley cultivars are resistant to stripe rust of wheat that is caused by Puccinia striiformis f. sp. tritici. The barley cv. Steptoe is susceptible to all identified races of P. striiformis f. sp. hordei (PSH), the barley stripe rust pathogen, but is resistant to most P. striiformis f. sp. tritici races. To determine inheritance of the Steptoe resistance to P. striiformis f. sp. tritici, a cross was made between Steptoe and Russell, a barley cultivar susceptible to some P. striiformis f. sp. tritici races and all tested P. striiformis f. sp. hordei races. Seedlings of parents and F1, BC1, F2, and F3 progeny from the barley cross were tested with P. striiformis f. sp. tritici races PST-41 and PST-45 under controlled greenhouse conditions. Genetic analyses of infection type data showed that Steptoe had one dominant gene and one recessive gene (provisionally designated as RpstS1 and rpstS2, respectively) for resistance to races PST-41 and PST-45. Genomic DNA was extracted from the parents and 150 F2 plants that were tested for rust reaction and grown for seed of F3 lines. The infection type data and polymorphic markers identified using the resistance gene analog polymorphism (RGAP) technique were analyzed with the Mapmaker computer program to map the resistance genes. The dominant resistance gene in Steptoe for resistance to P. striiformis f. sp. tritici races was mapped on barley chromosome 4H using a linked microsatellite marker, HVM68. A linkage group for the dominant gene was constructed with 12 RGAP markers and the microsatellite marker. The results show that resistance in barley to the wheat stripe rust pathogen is qualitatively inherited. These genes might provide useful resistance against wheat stripe rust when introgressed into wheat from barley.

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Quantitative Trait Loci for High-Temperature Adult-Plant and Slow-Rusting Resistance to Puccinia striiformis f. sp. tritici in Wheat Cultivars

Phytopathology ◽

10.1094/phyto-98-7-0803 ◽

2008 ◽

Vol 98 (7) ◽

pp. 803-809 ◽

Cited By ~ 43

Author(s):

Q. Guo ◽

Z. J. Zhang ◽

Y. B. Xu ◽

G. H. Li ◽

J. Feng ◽

...

Keyword(s):

High Temperature ◽

Stripe Rust ◽

Phenotypic Variation ◽

Quantitative Trait ◽

Puccinia Striiformis ◽

Infection Type ◽

Adult Plant ◽

Wheat Cultivars ◽

Slow Rusting ◽

Adult Plants

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most damaging diseases of wheat (Triticum aestivum) globally. High-temperature adult-plant resistance (HTAPR) and slow-rusting have great potential for sustainable management of the disease. The wheat cultivars Luke and Aquileja have been previously reported to possess HTAPR and slow-rusting to stripe rust, respectively. Aquileja displayed less number of stripes per unit leaf area than Luke, while Luke showed lower infection type than Aquileja at adult-plant stages of growth under high-temperature conditions. The objectives of this study were to confirm the resistances and to map the resistance genes in Luke and Aquileja. Luke was crossed with Aquileja, and 326 of the F2 plants were genotyped using 282 microsatellite primer pairs. These F2 plants and their derived F3 families were evaluated for resistance to stripe rust by inoculation in the fields and greenhouses of high- and low-temperatures. Infection type was recorded for both seedlings and adult plants, and stripe number was recorded for adult plants only. Two quantitative trait loci (QTL) were identified, on the short arm of chromosome 2B, to be significantly associated with infection type at adult-plant stages in the fields and in the high-temperature greenhouse. The locus distal to centromere, referred to as QYrlu.cau-2BS1, and the locus proximal to centromere, referred to as QYrlu.cau-2BS2, were separated by a genetic distance of about 23 cM. QYrlu.cau-2BS1 was flanked by the microsatellite markers Xwmc154 and Xgwm148, and QYrlu.cau-2BS2 was flanked by Xgwm148 and Xabrc167. QYrlu.cau-2BS1 and QYrlu.cau-2BS2 explained up to 36.6 and 41.5% of the phenotypic variation of infection type, respectively, and up to 78.1% collectively. No significant interaction between the two loci was detected. Another QTL, referred to as QYraq.cau-2BL, was detected on the long arm of chromosome 2B to be significantly associated with stripe number. QYraq.cau-2BL was flanked by the microsatellite markers Xwmc175 and Xwmc332, and it explained up to 61.5% of the phenotypic variation of stripe number. It is possible that these three QTL are previously unmapped loci for resistance to stripe rust.

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First Report of Stripe Rust caused by Puccinia striiformis f. sp. tritici on Wheat in Florida

Plant Health Progress ◽

10.1094/php-2005-0304-01-hn ◽

2005 ◽

Vol 6 (1) ◽

pp. 32 ◽

Cited By ~ 1

Author(s):

Ann R. Blount ◽

Shabbir A. Rizvi ◽

Ronald D. Barnett ◽

Xianming Chen ◽

Timothy S. Schubert ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Wheat Stripe Rust ◽

First Report ◽

Documented Case ◽

Rust Pathogen ◽

Wheat Lines ◽

Yield Trials

The wheat stripe rust pathogen occured on several experimental wheat lines planted at Quincy, FL in early February 2003. Several experimental lines in the 2003 Advanced Wheat A, the Advanced Wheat B, and the Uniform Southern Wheat Nursery yield trials then showed traces of stripe rust on the leaves of the plants. An unusually cool and wet winter and spring encouraged a scattered outbreak of stripe rust of wheat on susceptible experimental lines of wheat. This report constitutes the first documented case of stripe rust of wheat in Florida. Accepted for publication 22 February 2005. Published 4 March 2005.

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