Effect of Feed Supplemented with Selenium-Enriched Olive Leaves on Plasma Oxidative Status, Mineral Profile, and Leukocyte DNA Damage in Growing Rabbits

This study investigated the effect of a dietary combination of selenium and olive leaves on rabbit health status in order to evaluate the potential use of these combinations as functional ingredients in feed and food. Sixty weaning rabbits were fed with three diets: control feed (C), control feed + 10% normal olive leaves (OL), or olive leaves enriched in Se (2.17 mg Se/kg d.m.; SeOL). The plasma mineral profile, antioxidant status, and leukocyte DNA damage were determined. Inorganic Se was the most abundant form in the OL diet, while the organic one was higher in SeOL than C and OL. A similar trend was found in the plasma. Protein oxidation showed higher values in both supplemented groups; in addition, dietary Se led to a significant improvement (+40%) in ferric reducing ability of plasma (FRAP). A marked reduction in DNA damage (9-fold) was observed in the SeOL group compared to C. The combination of selenium and olive leaves in the diet of growing rabbits increased plasma SeMet and FRAP and reduced leukocyte DNA damage.

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Peripheral mononuclear leukocyte DNA damage, plasma prolidase activity, and oxidative status in patients with benign prostatic hyperplasia

Redox Report ◽

10.1179/1351000214y.0000000121 ◽

2014 ◽

Vol 20 (4) ◽

pp. 163-169 ◽

Cited By ~ 3

Author(s):

Ilhan Gecit ◽

Ismail Meral ◽

Mehmet Aslan ◽

Abdurrahim Kocyigit ◽

Hakim Celik ◽

...

Keyword(s):

Dna Damage ◽

Benign Prostatic Hyperplasia ◽

Oxidative Status ◽

Prostatic Hyperplasia ◽

Mononuclear Leukocyte ◽

Prolidase Activity ◽

Leukocyte Dna Damage

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Use of Selenium-enriched olive leaves in the feed of growing rabbits: Effect on oxidative status, mineral profile and Selenium speciation of Longissimus dorsi meat

Journal of Trace Elements in Medicine and Biology ◽

10.1016/j.jtemb.2018.10.004 ◽

2019 ◽

Vol 51 ◽

pp. 98-105 ◽

Cited By ~ 11

Author(s):

Simona Mattioli ◽

Alessandro Dal Bosco ◽

Joana Margarida Machado Duarte ◽

Roberto D’Amato ◽

Cesare Castellini ◽

...

Keyword(s):

Oxidative Status ◽

Longissimus Dorsi ◽

Selenium Speciation ◽

Olive Leaves ◽

Mineral Profile

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Oxidative status and DNA damage in operating room personnel

Clinical Biochemistry ◽

10.1016/j.clinbiochem.2008.09.103 ◽

2009 ◽

Vol 42 (3) ◽

pp. 189-193 ◽

Cited By ~ 17

Author(s):

Zeynep Baysal ◽

Mustafa Cengiz ◽

Abdullah Ozgonul ◽

Muslum Cakir ◽

Hakim Celik ◽

...

Keyword(s):

Dna Damage ◽

Operating Room ◽

Oxidative Status ◽

Operating Room Personnel

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Effects of plant extracts on antioxidant status and oxidant-induced stress in Caco-2 cells

British Journal Of Nutrition ◽

10.1017/s0007114507250469 ◽

2007 ◽

Vol 97 (2) ◽

pp. 321-328 ◽

Cited By ~ 59

Author(s):

S. Aisling Aherne ◽

Joseph P. Kerry ◽

Nora M. O'Brien

Keyword(s):

Dna Damage ◽

Plant Extract ◽

Plant Extracts ◽

Antioxidant Status ◽

Cell Injury ◽

Membrane Integrity ◽

Neutral Red ◽

Dna Integrity ◽

Cell Membrane Integrity ◽

Wide Range

Experimental evidence suggests that most herbs and spices possess a wide range of biological and pharmacological activities that may protect tissues against O2-induced damage. The objectives of the present study were: first, to determine the effects of plant extracts on the viability, membrane integrity, antioxidant status and DNA integrity of Caco-2 cells and second, to investigate the cytoprotective and genoprotective effects of these plant extracts against oxidative stress in Caco-2 cells. The plant extracts examined were rosemary (Rosmarinus officinalis L.), oregano (Origanum vulgare L.), sage (Salvia officinalis L.) and echinacea (Echinacea purpurea L.). Cell membrane integrity was assessed by the lactate dehydrogenase release assay. Viability was determined by the neutral red uptake assay (NRUA) and the concentration of compound that resulted in 50 % cell death (IC50) was calculated. Antioxidant status of the cells was assessed by measuring GSH content, catalase activity and superoxide dismutase activity. To examine their cytoprotective and genoprotective effects, Caco-2 cells were pre-treated with each plant extract for 24 h followed by exposure to H2O2. DNA damage was assessed by the comet assay and cell injury was determined by the NRUA. Rosemary was the most toxic (IC50 123 μg/ml) and echinacea the least toxic (IC50 1421 μg/ml). Sage was the only plant extract to affect the antioxidant status of the cells by increasing GSH content. Sage, oregano and rosemary protected against H2O2-induced DNA damage (olive tail moment and percentage tail DNA), whereas protection against H2O2-induced cytotoxicity was afforded by sage only.

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Salivary Antioxidant Status in Patients with Oral Lichen Planus: Correlation with Clinical Signs and Evolution during Treatment withChamaemelum nobile

BioMed Research International ◽

10.1155/2018/5187549 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 8

Author(s):

Asta Tvarijonaviciute ◽

Cristina Aznar-Cayuela ◽

Camila P. Rubio ◽

Fernando Tecles ◽

Jose J. Ceron ◽

...

Keyword(s):

Antioxidant Capacity ◽

Lichen Planus ◽

Antioxidant Status ◽

Oral Lichen Planus ◽

Clinical Signs ◽

Total Antioxidant Status ◽

Double Blind ◽

Reducing Ability ◽

Chamaemelum Nobile ◽

Oral Lichen

Oral lichen planus (OLP) is a chronic inflammatory mucocutaneous disease, which manifests as a succession of outbreaks. OLP was associated with salivary oxidative stress. Randomized, double blind, parallel-group study was performed. The sample consisted of 55 clinically and histopathologically diagnosed OLP patients. Twenty-six patients were treated with 2%Chamaemelum nobilegel and 29 with a placebo. Nonstimulated (basal) saliva was collected on the first day of the study and 4 weeks later. Salivary total antioxidant status (TAS) was evaluated by four different methods: two TAC (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) equivalent antioxidant capacity methods (TAC1 and TAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP). At baseline (T1), no statistically significant differences were detected in any of the TAS analytes between the two groups of patients. After four weeks of treatment, a statistically significant increase was detected in FRAP in the placebo group (0.323 [0.090–0.467] versus 0.406 [0.197–0.848] mmol/g⁎10-3) (P<0.05). Significant correlations were observed between pain and drainage and TAC1, CUPRAC, and FRAP and between xerostomia and the TAC1, TAC2, CUPRAC, and FRAP. The results of the present study showed that in patients with OLP increases of TAS in saliva are associated with increase in pain and xerostomia and decrease in drainage, suggesting a worsening condition of the patient. The use ofChamaemelum nobilegel would be recommended for disease stabilization.

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