Oxidative Stress, Antioxidant Status and DNA Damage in a Mercury Exposure Workers

Exposure to inhalation anesthetics (IAs) has been associated with DNA damage as reflected in the increased frequency of micronuclei (MN) and chromosomal aberrations (CAs). The present study was undertaken to ascertain whether there was any correlation between increased MN and CA and the extent of oxidative stress as well as the antioxidant status of a group of operating room personnel exposed to a mixture of IAs, including nitrous oxide, isoflurane, and sevoflurane. In this cross-sectional study, 60 operating room personnel (exposed group) in whom the frequencies of MN and CA had already been shown to be significantly higher than those of a referent group, as well as 60 unexposed nurses, were studied. Venous blood samples were taken from all participants, and malondialdehyde (MDA) levels as an index of oxidative stress (OS) and the activity of superoxide dismutase (SOD) and levels of total antioxidant capacity (TAC) as indices of antioxidant status were measured. The level of TAC (1.76 ± 0.59 mM vs. 2.13 ± 0.64 mM, p = 0.001) and the activity of SOD (11.22 ± 5.11 U/ml vs. 13.36 ± 4.12 U/ml, p = 0.01) were significantly lower, while the mean value of MDA was significantly higher (2.46 ± 0.66 µM vs. 2.19 ± 0.68 µM, p = 0.03) in the exposed group than in the nonexposed group. After adjusting for potential confounders, there were statistically significant associations between exposure to IAs, gender, SOD, and TAC with MN frequency and between exposure to IAs and SOD with numbers of CA. The findings of the present study indicated that exposure to IAs was associated with OS, and this, in turn, may be causally linked with DNA damage.

Download Full-text

Intrauterine exposure to flavonoids modifies antioxidant status at adulthood and decreases oxidative stress-induced DNA damage

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2012.12.021 ◽

2013 ◽

Vol 57 ◽

pp. 154-161 ◽

Cited By ~ 34

Author(s):

Kimberly Vanhees ◽

Frederik J. van Schooten ◽

Sahar Barjesteh van Waalwijk van Doorn-Khosrovani ◽

Stefan van Helden ◽

Armelle Munnia ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Antioxidant Status ◽

Intrauterine Exposure

Download Full-text

Assessment of DNA damage profile and oxidative/antioxidative biomarkers level in patients with inflammatory bowel disease

Polish Journal of Surgery ◽

10.5604/01.3001.0014.1548 ◽

2020 ◽

Vol 92 (4) ◽

pp. 1-5

Author(s):

Małgorzata Mrowicka ◽

Jerzy Mrowicki ◽

Michał Mik ◽

Łukasz Dziki ◽

Adam Dziki ◽

...

Keyword(s):

Oxidative Stress ◽

Inflammatory Bowel Disease ◽

Dna Damage ◽

Bowel Disease ◽

Healthy Subjects ◽

Antioxidant Status ◽

Oxidative Dna Damage ◽

Spectrophotometric Methods ◽

Inflammatory Bowel ◽

Repair Genes

Aim: The purpose of this study was to investigate the oxidative DNA damage, pro- antioxidant status in Polish patients with inflammatory bowel disease (IBD). Method: Oxidative DNA damage were measured by comet assay techniques; nitric oxide (NO) and plasmatic lipid peroxidation (MDA) as oxidative stress were valuated by colometric methods; superoxide dismutase (SOD1), catalase (CAT) and glutathione peroxidase (GPx1) as antioxidative defence were determined by spectrophotometric methods. Results: The level of oxidative DNA damage in IBD patients was significantly higher in relation to controls (P = 0.01). Alike, in control subject as well as in patients with IBD, lymphocytes are characterized by complete repair of DNA damage. A significant decrease of SOD (P = 0.031), CAT (P = 0.006), GPx1 (P = 0.001) activity was seen in IBD patients vs control. MDA (P = 0.001) and NO (P = 0.001) concentrations were significantly increased in IBD patients than in healthy subjects. Conclusion: Our results may be due to induction of DNA repair genes may occur at the stage of the pathological changes pathway (IBD), that may be caused by excessive oxidative stress. However, the reasons for these relationship, and whether it is direct or indirect, remains to be explored.

Download Full-text

Antioxidant status, oxidative stress and DNA damage in the aetiology of Malnutrition Related Diabetes Mellitus

Biochemical Society Transactions ◽

10.1042/bst025146s ◽

1997 ◽

Vol 25 (1) ◽

pp. 146S-146S ◽

Cited By ~ 9

Author(s):

MARGARET MCDONAGH ◽

LIAQUAT ALI ◽

AZAD KAHN ◽

PETER R. FLATT ◽

YVONNE A. BARNETT ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Dna Damage ◽

Antioxidant Status

Download Full-text

Effects of dietary extra virgin olive oil and its fractions on antioxidant status and DNA damage in the heart of rats co-exposed to aluminum and acrylamide

Food & Function ◽

10.1039/c5fo00342c ◽

2015 ◽

Vol 6 (9) ◽

pp. 3098-3108 ◽

Cited By ~ 17

Author(s):

Imen Ghorbel ◽

Mouna Khemakhem ◽

Ons Boudawara ◽

Rim Marrekchi ◽

Kamel Jamoussi ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Free Radicals ◽

Cardiovascular Diseases ◽

Olive Oil ◽

Antioxidant Status ◽

Virgin Olive Oil ◽

Extra Virgin Olive Oil ◽

Health Problems

Oxidative stress generated by an excessive production of free radicals has been linked to the development of several health problems such as cardiovascular diseases.

Download Full-text

ASSESSMENT OF DNA STRAND BREAKS AND TOTAL ANTIOXIDANT STATUS IN TYPE 2 DIABETIC PATIENTS WITH AND WITHOUT COMPLICATIONS- A CASE-CONTROL STUDY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i4.17020 ◽

2017 ◽

Vol 10 (4) ◽

pp. 430

Author(s):

K Nithya ◽

Isabel W ◽

Angeline T ◽

Priscilla As ◽

Asirvatham Aj

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Dna Damage ◽

Type 2 Diabetes Mellitus ◽

Antioxidant Status ◽

Total Antioxidant Status ◽

Diabetic Patients ◽

Total Antioxidant

Objective: To evaluate the total antioxidant status (TAS) and the extent of DNA strand breaks (damage) as a measure of oxidative stress biomarkers in Type 2 diabetic patients (with and without complications) and controls.Materials and Methods: Blood samples were collected from 200 patients with type 2 diabetes mellitus (n=100 with complications and n=100 without complications) and 100 healthy individuals. Oxidative DNA damage was evaluated using alkaline single cell gel electrophoresis (comet assay). Total antioxidant status was assessed by Ferric Reducing Ability of Plasma (FRAP) assay.Results: TAS was found to be significantly lower in type 2 diabetic patients (with and without complications) compared to controls (p< 0.001). Similarly, patients with complications of type 2 diabetes mellitus had significantly lower TAS when compared to diabetic patients (p= 0.007). DNA damage analysis showed that the extent of damage was high in patients with diabetes mellitus (with and without complications) compared to controls (p< 0.001). Fasting glucose and glycosylated haemoglobin level (HbA1c) was found to be significantly higherin diabetic patients than controls (p< 0.05). Correlation analysis showed that there is no association between age, duration, sugar level, HbA1c, TAS and DNA damage in patients with Type 2 diabetes mellitus.Conclusion: Alterations in TAS and the extent of DNA damage was observed in patients with complications of diabetes mellitus indicate that oxidative stress is more in patients with complications when compared to patients without complications and healthy individuals. Therefore, further DNA damage and onset of complications in Type 2 diabetes mellitus could be prevented by counteracting the oxidative stress by therapeutic interventions using appropriate antioxidants.Key words: Hyperglycemia, Oxidative stress, DNA damage, Total antioxidant status, Type 2 diabetes mellitus, Vascular complication

Download Full-text

Evaluation of oxidant-antioxidant balance and DNA damage in blood of patients with cancer of the head and neck under the influence of copper(II) complex: Preliminary studies

Postępy Higieny i Medycyny Doświadczalnej ◽

10.5604/01.3001.0014.4116 ◽

2020 ◽

Vol 74 ◽

pp. 382-390

Author(s):

Katarzyna Malinowska ◽

Alina Morawiec-Sztandera ◽

Małgorzata Majczyk ◽

Dariusz Kaczmarczyk ◽

Anna Merecz-Sadowska ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Head And Neck Cancer ◽

Head And Neck ◽

Neck Cancer ◽

Antioxidant Status ◽

Oxidative Dna Damage ◽

Total Antioxidant Status ◽

Healthy Controls ◽

Cancer Symptoms

Introduction: The primary aim of this research was to evaluate the oxidative stress markers and the level of oxidative DNA damage in the pathogenesis of head and neck cancer. Materials/Methods: Sixty-two subjects matched for age and gender, including 31 patients with head and neck cancer and 31 control patients without cancer symptoms, were enrolled in our study. In our work, the activity of antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX), as well as a total antioxidant status (TAS), were estimated. Additionally, an alkaline comet assay was used to measure the level of DNA damage in the group of patients with head and neck cancer and the group of healthy control patients. These tests were performed on a blood sample with and without prior incubation of dinitratebis (1-phenyl-5-(2-hydroxyphenyl)-3-methyl-N1pyrazol-κN2)cooper(II). Results: Significant increases of SOD, GPX CAT, TAS (P <0.001) were seen in blood from patients with head and neck cancer and prior incubation of cooper (II) component compared to blood from healthy controls without prior incubation of analyzed chemical. Moreover, we did not observe any relationship between the level of DNA damage and the studied component dinitratebis (1-phenyl-5-(2-hydroxyphenyl)-3-methyl-N1pyrazol-κN2)cooper(II) in the group of patients with head and neck cancer or in healthy controls. Discussion: Free radicals such as reactive oxygen species, which induce oxidative stress, may contribute to head and neck carcinogenesis. Therefore, we suggest that modulation of pro-oxidant /antioxidant status might be a relevant target for both prevention and therapy.

Download Full-text

Association between calcitriol and paricalcitol with oxidative stress in patients with hemodialysis

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000641 ◽

2020 ◽

pp. 1-8

Author(s):

Hasan Haci Yeter ◽

Berfu Korucu ◽

Elif Burcu Bali ◽

Ulver Derici

Keyword(s):

Oxidative Stress ◽

Vitamin D ◽

Antioxidant Status ◽

Total Antioxidant Status ◽

Stress Index ◽

Total Oxidant Status ◽

Vitamin D Analog ◽

Total Antioxidant ◽

Oxidant Status ◽

And Oxidative Stress

Abstract. Background: The pathophysiological basis of chronic kidney disease and its complications, including cardiovascular disease, are associated with chronic inflammation and oxidative stress. We investigated the effects of active vitamin D (calcitriol) and synthetic vitamin D analog (paricalcitol) on oxidative stress in hemodialysis patients. Methods: This cross-sectional study was composed of 83 patients with a minimum hemodialysis vintage of one year. Patients with a history of any infection, malignancy, and chronic inflammatory disease were excluded. Oxidative markers (total oxidant and antioxidant status) and inflammation markers (C-reactive protein and interleukin-6) were analyzed. Results: A total of 47% (39/83) patients were using active or analog vitamin D. Total antioxidant status was significantly higher in patients with using active or analog vitamin D than those who did not use (p = 0.006). Whereas, total oxidant status and oxidative stress index were significantly higher in patients with not using vitamin D when compared with the patients who were using vitamin D preparation (p = 0.005 and p = 0.004, respectively). On the other hand, total antioxidant status, total oxidant status, and oxidative stress index were similar between patients who used active vitamin D or vitamin D analog (p = 0.6; p = 0.4 and p = 0.7, respectively). Conclusion: The use of active or selective vitamin D analog in these patients decreases total oxidant status and increases total antioxidant status. Also, paricalcitol is as effective as calcitriol in decreasing total oxidant status and increasing total antioxidant status in patients with chronic kidney disease.

Download Full-text