scholarly journals Reproductive Toxicity of 3,4-dichloroaniline (3,4-DCA) on Javanese Medaka (Oryziasjavanicus, Bleeker 1854)

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 798
Author(s):  
Musa Adamu Ibrahim ◽  
Syaizwan Zahmir Zulkifli ◽  
Mohammad Noor Amal Azmai ◽  
Ferdaus Mohamat-Yusuff ◽  
Ahmad Ismail
Keyword(s):  
Aquatic Ecosystems ◽  
Cell Structure ◽  
Gonadosomatic Index ◽  
Reproductive Toxicity ◽  
Exposed Group ◽  
Oryzias Javanicus ◽  
Reproductive Response ◽  
Aquatic Vertebrate ◽  
Vertebrate Model ◽  
Javanese Medaka

Compound 3,4-dichloroaniline (3,4-DCA) is a metabolite of several urea herbicides and intermediate chemical of several industrial products. Moreover, 3,4-DCA has been frequently detected in aquatic ecosystems around the world. This aniline is more toxic than the parent chemicals, and it affects non-target organisms. This study evaluated a 21-day reproductive response of an emerging aquatic vertebrate model, Javanese medaka (Oryzias javanicus), exposed to 3,4-DCA. Fecundity and gonads histopathology were observed. The spawning rate and fertilisation reduced significantly in the highest exposed-group (250 µg/L). Gonadosomatic index (GSI) was significantly low in females exposed to 250 µg/L. No substantial structural alteration of male gonads. However, oocyte development and ovarian cell structure were disrupted in 250 µg/L exposed females. The gonadal developmental was not affected in the males; however, a significant reduction in the developmental of female gonads was observed at 250 µg/L. These results show that 3,4-DCA interfere with the reproduction of Javanese medaka through fecundity and alteration of gonadal tissues.

scholarly journals Herbicide Diuron as Endocrine Disrupting Chemicals (EDCs) through Histopathalogical Analysis in Gonads of Javanese Medaka (Oryzias javanicus, Bleeker 1854)

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 525 ◽  
Author(s):  
Nur Amiera Kamarudin ◽  
Syaizwan Zahmir Zulkifli ◽  
Mohammad Noor Amal Azmai ◽  
Fatin Zahidah Abdul Aziz ◽  
Ahmad Ismail
Keyword(s):  
Negative Impact ◽  
Endocrine Disrupting Chemicals ◽  
Aquatic Organism ◽  
Long Term Effects ◽  
Oryzias Javanicus ◽  
Agriculture Industry ◽  
Significant Negative Impact ◽  
Javanese Medaka ◽  
Herbicide Diuron

The expeditious augmentation of the agriculture industry is leaving a significant negative impact on aquatic ecosystems. However, the awareness of the impacts of herbicide Diuron toxicities on the non-targeted aquatic organism, especially fish is still lacking. Javanese medaka, a new model fish species were exposed under sublethal levels and the long-term effects on gonads were investigated via histological studies. A total of 210 sexually mature fish were exposed to Diuron at seven different concentrations; control, solvent control, 1, 50, 100, 500, and 1000 μg/L for 21 days. In this study, Diuron caused histopathological alterations in gonads (ovary and testis) of Javanese medaka (Oryzias javanicus) by decreasing in gonadal staging and maturity of germ cells in oogenesis and spermatogenesis of female and male Javanese medaka. The results obtained in this study had proven our hypothesis that long-term exposure of herbicide Diuron can cause alterations in the gonadal histology of the adults of Javanese medaka.

scholarly journals Detection of Metallothionein in Javanese Medaka (Oryzias javanicus), Using a scFv-Immobilized Protein Chip

Sensors ◽  
2018 ◽  
Vol 18 (4) ◽  
pp. 1069 ◽  
Author(s):  
Euiyeon Lee ◽  
Hyunjin Jeon ◽  
Chungwon Kang ◽  
Seonock Woo ◽  
Seungshic Yum ◽  
...  
Keyword(s):  
Protein Chip ◽  
Oryzias Javanicus ◽  
Javanese Medaka

scholarly journals cDNA cloning, characterization and expression of cytochrome P450 family 1 (CYP1A) from Javanese medaka, Oryzias javanicus by environmental conditions

2014 ◽  
Vol 13 (18) ◽  
pp. 1898-1909 ◽  
Author(s):  
Tuan Trieu ◽  
Kaminishi Yoshino ◽  
Funahashi Aki ◽  
A.H. Mohamed El-Kady ◽  
A. I. Hassanin Abeer ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cdna Cloning ◽  
Environmental Conditions ◽  
Oryzias Javanicus ◽  
Javanese Medaka ◽  
Cytochrome P450 Family

scholarly journals The effects of fish gender on susceptibility to acute Streptococcus agalactiae infection in Javanese medaka Oryzias javanicus

2018 ◽  
Vol 114 ◽  
pp. 251-254 ◽  
Author(s):  
M.N.A. Amal ◽  
S.T. Zarif ◽  
M.S. Suhaiba ◽  
M.R.M. Aidil ◽  
N.N. Shaqinah ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Oryzias Javanicus ◽  
Javanese Medaka

scholarly journals Toxicity of Zinc Oxide Nanoparticles on the Embryo of Javanese Medaka (Oryzias javanicus Bleeker, 1854): A Comparative Study

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2170
Author(s):  
Naweedullah Amin ◽  
Syaizwan Zahmir Zulkifli ◽  
Mohammad Noor Amal Azmai ◽  
Ahmad Ismail
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Tap Water ◽  
Pure Water ◽  
High Sensitivity ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Ultra Pure Water ◽  
Oryzias Javanicus ◽  
Javanese Medaka

(1) Background: Zinc oxide nanoparticles (ZnO NPs) are widely applied in various human products. However, they can be extremely toxic for aquatic organisms, particularly fish. This research was conducted to determine the LC50 of ZnO NPs on the embryos of Javanese medaka (Oryzias javanicus) in ultra-pure, deionized, and dechlorinated tap water; (2) Methods: The experiments were conducted in a completely randomized design (CRD) with three replicates for six treatments for acute (0.100, 0.250, 0.500, 1.00, 5.00, and 10.00 mg/L) exposures for each type of water; (3) Results: The LC50 of ZnO NPs at 96 h was determined as 0.643 mg/L in ultra-pure water, 1.333 mg/L in deionized water, and 2.370 in dechlorinated tap water. In addition to concentration-dependent toxicity, we also observed time-dependent toxicity for ZnO NPs. In addition, the sizes of ZnO NPs increased immediately after dispersion and were 1079 nm, 3209 nm, and 3652 nm in ultra-pure, deionized, and dechlorinated tap water. The highest concentration of measured Zn2+ in exposure concentrations was found in ultra-pure water, followed by deionized and dechlorinated tap water suspensions. Furthermore, Javanese medaka showed high sensitivity to acute exposure of ZnO NPs in all types of water.

scholarly journals Behavioral Responses of Javanese Medaka (Oryzias Javanicus) Versus Zebrafish (Danio Rerio) in Open Field Test.

2020 ◽  
Author(s):  
Nurul Syafida Asma’ Mohd Sataa ◽  
Noraini Abu Bakar ◽  
Nur Atikah Saleh Hodin ◽  
Nurul Farhana Ramlan ◽  
Wan Norhamidah Wan Ibrahim
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Behavioral Responses ◽  
Model Organisms ◽  
Home Base ◽  
Behavioral Phenotypes ◽  
Oryzias Javanicus ◽  
Field Environment ◽  
Javanese Medaka

Abstract Background: Locomotion is integral for animal survivability. However, the understandings of locomotor that lead to exhibition of multiple complex behaviors of fish models in response to an open field environment still remain unresolved. To determine whether two different fish models, Javanese medaka and zebrafish have similar baseline locomotor activity in open field paradigm, an open field test was used. Results: Results showed that Javanese medaka exhibit increased in exploratory activity with lower anxiety responses; exhibit a steady habituation response in OFT paradigm and vice versa in zebrafish. Medaka also took longer duration to establish home-base in comparison to the zebrafish. Although no other motor responses were observed, both fish species displayed strong preference of left eye used to assess the OFT tank. Conclusion: Medaka exhibits slower locomotors activity, lower anxiety responses and steadily maintains its locomotion once they reached habituation. In comparison, zebrafish demonstrated bolder behavioral phenotypes where they showed faster locomotors activity, higher anxiety responses with similar habituation response to the Javanese medaka. Thus, this present study revealed that two different teleost aquatic model organisms, Javanese medaka and zebrafish have different behavioral phenotypes in open field test.

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