scholarly journals Maternal and Neonatal Evaluation of Derived Reactive Oxygen Metabolites and Biological Antioxidant Potential in Donkey Mares and Foals

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2885
Author(s):  
Micaela Sgorbini ◽  
Francesca Bonelli ◽  
Giulia Percacini ◽  
Anna Pasquini ◽  
Alessandra Rota

Our aim was to measure the concentrations of derived reactive oxygen metabolite (d-ROMs) and biological antioxidant potential (BAP) of donkey mares and foals at delivery and to verify the protective role of the placenta against fetal oxidative stress. A total of 15 Amiata jennies with a physiological gestation length and delivery were included together with 17 foals (two twin foalings). After delivery, maternal and foal venous blood samples were collected along with blood from the artery. Circulating lactate and plasma d-ROMs and BAP were evaluated. The Wilcoxon test for paired data was applied to verify differences in d-ROMs and BAP values, while the Spearman test was used to evaluate correlations. A significantly higher d-ROMs concentration was found in jennies compared to their foals, and to the umbilical artery blood. The BAP was higher in jennies than in their foals, but no differences were observed in the umbilical artery blood. No difference was found between foals and their umbilical cord. Blood lactate was higher in foals than in their dams. Positive correlations were found between mares and umbilical cord for BAP and d-ROMs, and between mares and foals and umbilical cord for BAP. In conclusion, the placenta may be a protective factor for the fetus. As with equine foals, the antioxidant system of donkey foals does not seems to be effective at birth.

Healthcare ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 368
Author(s):  
Rosamaria Militello ◽  
Simone Luti ◽  
Matteo Parri ◽  
Riccardo Marzocchini ◽  
Riccardo Soldaini ◽  
...  

Background: Most studies on oxidative stress markers and antioxidant levels have been conducted in male athletes, although female participation in sport has increased rapidly in the past few decades. In particular, it could be important to assess oxidative stress markers in relation to the training load because the anaerobic path becomes predominant in high-intensity actions. Methods: Ten female professional basketball players, performing five 2 h-lasting training sessions per week, and 10 sedentary control women were investigated. Capillary blood and saliva samples were collected in the morning before the training session. The antioxidant capacity and the levels of reactive oxygen metabolites on plasma were determined measuring Reactive Oxygen Metabolite and Biological Antioxidant Potential (d-ROMs and the BAP Test). Salivary cortisol was detected by using commercial enzyme-linked immunosorbent assay kit. Results: The antioxidant capacity (BAP value) was significantly higher in elite basketball players (21.2%; p < 0.05). Conversely, cortisol (51%; p < 0.009) and the levels of oxidative species (d-ROM, 21.9%; p < 0.05) showed a significant decrease in elite athletes.


PLoS ONE ◽  
2020 ◽  
Vol 15 (5) ◽  
pp. e0233550
Author(s):  
Masahito Morimoto ◽  
Toshiaki Hashimoto ◽  
Yoshimi Tsuda ◽  
Tadanori Nakatsu ◽  
Taisuke Kitaoka ◽  
...  

Retos ◽  
2020 ◽  
pp. 483-487
Author(s):  
Andres Ariza Viviescas ◽  
Diana Marcela Niño Pinzón ◽  
Hugo Celso Dutra de Souza ◽  
Juan Daniel Esteban Moreno ◽  
Diego Benítez Medina ◽  
...  

Abstract. Introduction: There is few evidence that details the behavior of each spatiotemporal variable of the running pattern of female soccer players using different surfaces. Objective: To describe the spatiotemporal variables of the sprint pattern developed on natural and artificial turf by professional female soccer players. Methods: A cross-sectional study was conducted on nineteen (n=19) professional athletes with an average age of 22.3 years, who´s sprint spatiotemporal variables were evaluated in a natural (Bermuda 419) and artificial (Star 2) playing field through an optical measurement system (Opto Gait, Italy). The analysis of the differences of the space and time variables by turf was done with the Wilcoxon test for paired data and the differences in speed and acceleration by playing position was done using the Kruskal–Wallis test. The Spearman test was used to compare the correlation between speed, acceleration and anthropometric variables. Finally, an alpha level of 5% was considered for the whole analysis. Results: On the natural turf, the speed and cadence of the players were higher; on the artificial turf, the energy, flight time, contact phase and step angle were higher (p<0.05). On the other hand, an indirect relationship was observed between speed, contact time, percentage and fat weight (p=0.01). Conclusion: Our results suggest that the natural turf, Bermuda 419, allows for a faster sprinting patterns, characterized by lower energy use, flight time, contact phase and step angle.  Resumen. Introducción: Existe poca evidencia que detalle el comportamiento de cada variable espaciotemporal del patrón de carrera de mujeres futbolistas utilizando diferentes superficies. Objetivo: describir las variables espacio temporales del patrón de carrera desarrollado en superficie natural y artificial por mujeres futbolistas profesionales. Métodos: se realizó un estudio de corte transversal en diecinueve (n=19) deportistas profesionales con un promedio de edad de 22.3 años, a quienes se les evaluaron las variables espacio temporales de la carrera en césped natural (Bermuda 419) y artificial (Star 2) a través de un sistema de medición óptico (Opto Gait). El análisis de las diferencias de las variables espacio temporales fue realizado con el test de Wilcoxon para datos pareados y las diferencias en la velocidad y aceleración fue realizados usando el test Kruskal-Wallis. El test de Spearman se usó para comparar la correlación entre las variables de velocidad, aceleración y antropométricas. Finalmente, un nivel de alpha de 5% fue considerado para el análisis total. Resultados: en la superficie natural, la velocidad y cadencia de las futbolistas fueron superiores; sobre superficie artificial, la energía, el tiempo de vuelo, la fase de contacto y el ángulo de paso fueron más altas (p<0.05). De otra parte, se observó una relación indirecta entre la velocidad, tiempo de contacto, porcentaje y peso graso (p=0.01). Conclusión: nuestros resultados sugieren que la superficie natural, Bermuda 419, permite un patrón de carrera más rápido, caracterizado por un menor uso de energía, tiempo de vuelo, fase de contacto y ángulo de paso.


1991 ◽  
Vol 260 (1) ◽  
pp. H42-H49
Author(s):  
I. M. Dauber ◽  
E. J. Lesnefsky ◽  
K. M. VanBenthuysen ◽  
J. V. Weil ◽  
L. D. Horwitz

The role of reactive oxygen metabolites in ischemia-reperfusion coronary microvascular injury is unclear. To investigate this problem, we tested the effects of the reactive oxygen metabolite scavengers superoxide dismutase (SOD) and dimethylthiourea (DMTU) on ischemia-reperfusion-induced coronary microvascular dysfunction. As an index of vascular function, we assessed microvascular permeability with a double radioisotope protein leak index (PLI) method. Anesthetized dogs underwent 60 min of ischemia via left anterior descending (LAD) occlusion followed by 60 min of reperfusion. Untreated animals (n = 7) received saline. SOD-treated animals (n = 6) received 140 U.kg-1.min-1 (6.6 mg.kg-1.min-1) bovine SOD throughout ischemia and reperfusion. DMTU-treated animals (n = 5) received a 500 mg/kg bolus 30 min before ischemia. At the beginning of reperfusion, radiolabeled autologous protein (113mIn transferrin) and red blood cells (99mTc) were given intravenously for the assessment of permeability. In untreated dogs, ischemia-reperfusion increased the PLI of ischemic (flow less than 20 ml.min-1.100 g-1) myocardium more than threefold compared with that of nonischemic (flow greater than 100 ml.min-1.100 g-1) myocardium (ischemic-to-nonischemic PLI ratio = 3.49 +/- 0.48). SOD reduced the PLI of ischemic myocardium by 45% and DMTU reduced it by 66% (PLI = 9.25 +/- 1.30, 5.04 +/- 1.18, and 3.16 +/- 0.94, untreated, SOD, and DMTU, respectively). The PLI was increased proportional to the regional severity of ischemic blood flow. Both SOD and DMTU reduced the increase in protein leak at all levels of regional ischemic blood flow. Neither SOD nor DMTU increased regional myocardial blood flow to the occluded LAD zone.(ABSTRACT TRUNCATED AT 250 WORDS)


2011 ◽  
Vol 75 (10) ◽  
pp. 2453-2459 ◽  
Author(s):  
Tomoyo Yahata ◽  
Chinatsu Suzuki ◽  
Akiko Hamaoka ◽  
Maiko Fujii ◽  
Kenji Hamaoka

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