scholarly journals A Z-Linked E3 Ubiquitin Ligase Cs-rchy1 Is Involved in Gametogenesis in Chinese Tongue Sole, Cynoglossus semilaevis

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3265
Author(s):  
Yuxuan Sun ◽  
Ying Zhu ◽  
Peng Cheng ◽  
Mengqian Zhang ◽  
Na Wang ◽  
...  
Keyword(s):  
Cell Line ◽  
Ubiquitin Ligase ◽  
Developmental Stages ◽  
Gonadal Development ◽  
Cynoglossus Semilaevis ◽  
Z Chromosome ◽  
Hormone Synthesis ◽  
Tongue Sole ◽  
Ubiquitin Ligase E3

Ubiquitin ligase (E3) plays a versatile role in gonadal development and spermatogenesis in mammals, while its function in fish is little reported. In this study, a Z-chromosome linked ubiquitin ligase rchy1 in C. semilaevis (Cs-rchy1) was cloned and characterized. The full-length cDNA was composed of 1962 bp, including 551 bp 5′UTR, 736 bp 3′UTR, and 675 bp ORF encoding a 224-amino-acid (aa) protein. Cs-rchy1 was examined among seven different tissues and found to be predominantly expressed in gonads. In testis, Cs-rchy1 could be detected from 40 days post hatching (dph) until 3 years post hatching (yph), but there was a significant increase at 6 months post hatching (mph). In comparison, the expression levels in ovary were rather stable among different developmental stages. In situ hybridization showed that Cs-rchy1 was mainly localized in germ cells, that is, spermatid and spermatozoa in testis and stage I, II and III oocytes in ovary. In vitro RNA interference found that Cs-rchy1 knockdown resulted in the decline of sox9 and igf1 in ovarian cell line and down-regulation of cyp19a in the testicular cell line. These data suggested that Cs-rchy1 might participate in gonadal differentiation and gametogenesis, via regulating steroid hormone synthesis.

Establishment and characterization of an ovarian cell line from half-smooth tongue sole Cynoglossus semilaevis

2014 ◽  
Vol 86 (1) ◽  
pp. 46-59 ◽  
Author(s):  
A. Sun ◽  
T. Z. Wang ◽  
N. Wang ◽  
X. F. Liu ◽  
Z. X. Sha ◽  
...  
Keyword(s):  
Cell Line ◽  
Cynoglossus Semilaevis ◽  
Ovarian Cell ◽  
Tongue Sole ◽  
Half Smooth Tongue Sole

scholarly journals Establishment and Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole, Cynoglossus semilaevis

2011 ◽  
Vol 7 (4) ◽  
pp. 452-459 ◽  
Author(s):  
Bo Zhang ◽  
Xianli Wang ◽  
Zhenxia Sha ◽  
Changgeng Yang ◽  
Shanshan Liu ◽  
...  
Keyword(s):  
Cell Line ◽  
Cynoglossus Semilaevis ◽  
Testicular Cell ◽  
Tongue Sole ◽  
Half Smooth Tongue Sole

scholarly journals The Yeast GID Complex, a Novel Ubiquitin Ligase (E3) Involved in the Regulation of Carbohydrate Metabolism

2008 ◽  
Vol 19 (8) ◽  
pp. 3323-3333 ◽  
Author(s):  
Olivier Santt ◽  
Thorsten Pfirrmann ◽  
Bernhard Braun ◽  
Jeannette Juretschke ◽  
Philipp Kimmig ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Ubiquitin Ligase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Ring Finger ◽  
Vitro Assay ◽  
Fructose 1,6 Bisphosphatase ◽  
Key Enzyme ◽  
Ubiquitin Ligase E3

Glucose-dependent regulation of carbon metabolism is a subject of intensive studies. We have previously shown that the switch from gluconeogenesis to glycolysis is associated with ubiquitin-proteasome linked elimination of the key enzyme fructose-1,6-bisphosphatase. Seven glucose induced degradation deficient (Gid)-proteins found previously in a genomic screen were shown to form a complex that binds FBPase. One of the subunits, Gid2/Rmd5, contains a degenerated RING finger domain. In an in vitro assay, heterologous expression of GST-Gid2 leads to polyubiquitination of proteins. In addition, we show that a mutation in the degenerated RING domain of Gid2/Rmd5 abolishes fructose-1,6-bisphosphatase polyubiquitination and elimination in vivo. Six Gid proteins are present in gluconeogenic cells. A seventh protein, Gid4/Vid24, occurs upon glucose addition to gluconeogenic cells and is afterwards eliminated. Forcing abnormal expression of Gid4/Vid24 in gluconeogenic cells leads to fructose-1,6-bisphosphatase degradation. This suggests that Gid4/Vid24 initiates fructose-1,6-bisphosphatase polyubiquitination by the Gid complex and its subsequent elimination by the proteasome. We also show that an additional gluconeogenic enzyme, phosphoenolpyruvate carboxykinase, is subject to Gid complex-dependent degradation. Our study uncovers a new type of ubiquitin ligase complex composed of novel subunits involved in carbohydrate metabolism and identifies Gid4/Vid24 as a major regulator of this E3.

Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)

2010 ◽  
Vol 29 (2) ◽  
pp. 81-87 ◽  
Author(s):  
Zhenxia Sha ◽  
Guocheng Ren ◽  
Xianli Wang ◽  
Na Wang ◽  
Songlin Chen
Keyword(s):  
Cell Line ◽  
Cynoglossus Semilaevis ◽  
Tongue Sole ◽  
A Cell ◽  
Half Smooth Tongue Sole

scholarly journals Effects of different dietary DHA:EPA ratios on gonadal steroidogenesis in the marine teleost, tongue sole (Cynoglossus semilaevis)

2017 ◽  
Vol 118 (3) ◽  
pp. 179-188 ◽  
Author(s):  
Houguo Xu ◽  
Lin Cao ◽  
Yuliang Wei ◽  
Yuanqin Zhang ◽  
Mengqing Liang
Keyword(s):  
Mrna Expression ◽  
Regulatory Protein ◽  
Mature Female ◽  
Cynoglossus Semilaevis ◽  
Marine Teleost ◽  
Sex Steroid Hormone ◽  
Hormone Synthesis ◽  
Tongue Sole ◽  
Chain Cleavage ◽  
Steroidogenic Acute Regulatory

AbstractThe present study was conducted to investigate the effects of dietary DHA and EPA on gonadal steroidogenesis in mature females and males, with a feeding trial on tongue sole, a typical marine teleost with sexual dimorphism. Three experimental diets differing basically in DHA:EPA ratio, that is, 0·68 (diet D:E-0·68), 1·09 (D:E-1·09) and 2·05 (D:E-2·05), were randomly assigned to nine tanks of 3-year-old tongue sole (ten females and fifteen males in each tank). The feeding trail lasted for 90 d before and during the spawning season. Fish were reared in a flowing seawater system and fed to apparent satiation twice daily. Compared with diet D:E-0·68, diet D:E-1·09 significantly enhanced the oestradiol production in females, whereas diet D:E-2·05 significantly enhanced the testosterone production in males. In ovaries, diet D:E-1·09 induced highest mRNA expression of follicle-stimulating hormone receptor (FSHR), steroidogenic acute regulatory protein, 17α-hydroxylase (P450c17) and 3β-hydroxysteroid dehydrogenase (3β-HSD). In testes, diet 2·05 resulted in highest mRNA expression of FSHR, cholesterol side-chain cleavage enzyme, P450c17 and 3β-HSD. Fatty acid profiles in fish tissues reflected closely those of diets. Female fish had more gonadal EPA content but less DHA content than male fish, whereas there was a reverse observation in liver. In conclusion, the dietary DHA:EPA ratio, possibly combined with the dietary EPA:arachidonic acid ratio, differentially regulated sex steroid hormone synthesis in mature female and male tongue soles. Females seemed to require more EPA but less DHA for the gonadal steroidogenesis than males. The results are beneficial to sex-specific nutritive strategies in domestic teleost.

Establishment of a continuous cell line from female half-smooth tongue sole (Cynoglossus semilaevis)

2015 ◽  
Vol 47 (11) ◽  
pp. 3608-3615
Author(s):  
Yuan Zheng ◽  
Na Wang ◽  
Zhen-Xia Sha ◽  
Song-Lin Chen
Keyword(s):  
Cell Line ◽  
Cynoglossus Semilaevis ◽  
Continuous Cell Line ◽  
Tongue Sole ◽  
Half Smooth Tongue Sole
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