scholarly journals Immunohistochemical Detection of Encephalitozoon cuniculi in Ocular Structures of Immunocompetent Rabbits

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 988
Author(s):  
Edita Jeklová ◽  
Lenka Levá ◽  
Vladimír Kummer ◽  
Vladimír Jekl ◽  
Martin Faldyna
Keyword(s):  
Infectious Disease ◽  
Clinical Signs ◽  
Oral Infection ◽  
Immunohistochemical Detection ◽  
Encephalitozoon Cuniculi ◽  
Emerging Pathogen ◽  
Ocular Tissues ◽  
Inflammatory Lesions ◽  
Common Infectious Disease ◽  
Immunocompromised Hosts

Encephalitozoonosis is a common infectious disease widely spread among rabbits. Its causative agent, Encephalitozoon cuniculi, is considered as a zoonotic and emerging pathogen capable of infecting both immunocompetent and immunocompromised hosts, including humans. In rabbits, clinical signs include neurological, kidney and ocular disease. The aim of this study was to detect E. cuniculi in ocular structures in immunocompetent rabbits after experimental oral infection using immunohistochemistry. In infected animals, E. cuniculi spores were present in periocular connective tissue, sclera, cornea, choroidea, iris, retina and lens, as a round to ovoid organism reacting with a specific anti-E. cuniculi monoclonal antibody as early as 2 weeks after infection. There were no signs of inflammatory lesions in any of the ocular tissues examined at 2, 4, 6 and 8 weeks after infection. In the present study, E. cuniculi was also detected in the lenses of adult rabbits, which indicates that ways of lens infection other than intrauterine and haematogenic are possible.

scholarly journals Encephalitozoon cuniculi: Grading the Histological Lesions in Brain, Kidney, and Liver during Primoinfection Outbreak in Rabbits

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Luis E. Rodríguez-Tovar ◽  
Alicia M. Nevárez-Garza ◽  
Armando Trejo-Chávez ◽  
Carlos A. Hernández-Martínez ◽  
Gustavo Hernández-Vidal ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Renal Medulla ◽  
Human Consumption ◽  
Encephalitozoon Cuniculi ◽  
Emerging Pathogen ◽  
Cerebral Lesions ◽  
Northern Mexico ◽  
Type C ◽  
First Time ◽  
Grade Iii

This is the first confirmed report ofEncephalitozoon cuniculi(E. cuniculi) in farm meat rabbits located in Northern Mexico. Eighty young rabbits exhibited clinical signs of this zoonotic emerging disease, like torticollis, ataxia, paresis, circling, and rolling. Samples of brain, kidney, and liver were examined for histology lesions. For the first time the lesions caused byE. cuniculiwere graded according to their severity (I, II, and III) and the size of the granulomas (Types A, B, and C). The main cerebral injuries were Grade III, coinciding with the presence of Type C granulomas. The cerebral lesions were located in the cortex, brain stem, and medulla. The renal lesions were also Grade III distributed throughout cortex and renal medulla, with no granuloma formation. The involvement of hypersensitivity Types III and IV is suggested. All of the rabbits were seropositive toE. cuniculiby CIA testing, suggesting that this zoonotic and emerging pathogen is widely distributed among animals intended for human consumption. We believe this work could be used as a guide when examiningE. cuniculiand will provide direction to confirm the diagnosis of this pathogen.

COVID-19: how nuclear medicine can provide a differential diagnosis in very dubious case

Coronaviruses ◽  
2020 ◽  
Vol 01 ◽  
Author(s):  
Maria Silvia De Feo ◽  
Viviana Frantellizzi ◽  
Giuseppe De Vincentis
Keyword(s):  
Infectious Disease ◽  
Differential Diagnosis ◽  
Ct Scan ◽  
Imaging Technique ◽  
False Negative ◽  
Clinical Signs ◽  
Negative Results ◽  
Infectious Disease Department ◽  
False Negative Results ◽  
99Mtc Hmpao

Background: We present the case of a 55-year-old woman, admitted to the Infectious Disease Department of Policlinico Umberto I, Rome, in mid-March 2020, with suspicion of COVID-19 infection. Objective: The rRT-PCR was negative and the following CT scan, performed to exclude false-negative results and help diagnosis, was inconclusive. Methods: It was decided to submit the patient to 99mTc-HMPAO-labelled leukocyte scan. Results: This exam led to the diagnosis of infective endocarditis. Conclusion: In the present pandemic scenario, 99mTc-HMPAO-labelled leukocyte scan represents a reliable imaging technique for differential diagnosis with COVID-19 in patients with confusing clinical signs, possible false-negative rRT-PCR results and inconclusive CT scan.

scholarly journals Non-specific effects of rabies vaccine on the incidence of common infectious disease episodes: study protocol for a randomized controlled trial

Trials ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Darryn Knobel ◽  
Christianah Ibironke Odita ◽  
Anne Conan ◽  
Donna Barry ◽  
Marshalette Smith-Anthony ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Randomized Controlled Trial ◽  
Study Protocol ◽  
Controlled Trial ◽  
Rabies Vaccine ◽  
Randomized Controlled ◽  
Specific Effects ◽  
Common Infectious Disease

scholarly journals Voriconazole-Resistant Penicillium oxalicum: An Emerging Pathogen in Immunocompromised Hosts

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Anuradha Chowdhary ◽  
Shallu Kathuria ◽  
Kshitij Agarwal ◽  
Neelam Sachdeva ◽  
Pradeep K. Singh ◽  
...  
Keyword(s):  
Myeloid Leukemia ◽  
Penicillium Oxalicum ◽  
Chronic Obstructive ◽  
Emerging Pathogen ◽  
Penicillium Species ◽  
Obstructive Pulmonary Disease ◽  
Invasive Mycosis ◽  
Invasive Mycoses ◽  
Immunocompromised Hosts ◽  
Acute Myeloid

Abstract Penicillium species are rarely reported agents of infections in immunocompromised patients. We report 3 cases of invasive mycosis caused by voriconazole-resistant Penicillium oxalicum in patients with acute myeloid leukemia, diabetes mellitus, and chronic obstructive pulmonary disease, while on voriconazole therapy. Penicillium oxalicum has not been previously recognized as a cause of invasive mycoses.

scholarly journals A Case Report of Splenic Tuberculous Abscess

2018 ◽  
Vol 7 (1) ◽  
pp. 23-25
Author(s):  
Manoj Kumar Sah ◽  
Bhawesh Thapa ◽  
Samrat Shrestha
Keyword(s):  
Infectious Disease ◽  
Case Report ◽  
South Asian ◽  
Positive Patient ◽  
Rare Clinical Entity ◽  
Antitubercular Treatment ◽  
Common Infectious Disease ◽  
Tuberculous Abscess ◽  
Splenic Tuberculosis ◽  
South Asian Region

Tuberculosis is a common infectious disease in South-Asian region which can involve different organs. Splenic tuberculosis is a rare clinical entity. Here we report a case of tuberculous abscess of spleen in a 72 year old diabetic male. It was disseminated type, it was confirmed by ultrasonography guided aspiration of abscess for gene x-pert test, which was positive. Patient was given antitubercular treatment after the confirmation of the diagnosis and is improving with given treatment.  

Bilateral facial palsy in an older person

2020 ◽  
Vol 49 (5) ◽  
pp. 887-888
Author(s):  
Jean-Loup Méreaux ◽  
Benjamin Hébant ◽  
Nicolas Magne ◽  
Gérald Quesney ◽  
Romain Lefaucheur
Keyword(s):  
Infectious Disease ◽  
Lyme Disease ◽  
Facial Palsy ◽  
Related Species ◽  
Older Patients ◽  
Clinical Signs ◽  
Older Person ◽  
Older Patient ◽  
Infected Tick ◽  
Outdoor Activities

Abstract Lyme disease is an infectious disease caused by the Borrelia burgdorferi spirochetes and other related species that are transmitted through an infected tick bite. We report the case of an older patient presenting with bilateral facial palsy due to Lyme disease. Multiple non-specific clinical signs preceded facial palsy with falls, fatigue and pain of both legs especially during the night. Our case illustrates the difficulty to diagnose this infectious disease, especially in older patients who have rare outdoor activities and a low risk of tick exposure.

Immunohistochemical Detection of Canine Adenovirus in Paraffin Sections of Liver

1986 ◽  
Vol 23 (4) ◽  
pp. 478-484 ◽  
Author(s):  
P. M. Rakich ◽  
K. W. Prasse ◽  
P. D. Lukert ◽  
L. M. Cornelius
Keyword(s):  
Immunohistochemical Detection ◽  
Hepatic Inflammation ◽  
Paraffin Sections ◽  
Inflammatory Lesions ◽  
Long Term Stability ◽  
Formalin Fixed Paraffin ◽  
Wide Range ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

An avidin-biotin immunoperoxidase procedure was optimized for detection of canine adenoviral antigens in formalin-fixed, paraffin-embedded liver. Long-term stability of viral antigen was shown by successful demonstration of virus in liver tissue preserved up to six years from dogs with infectious canine hepatitis. This immunohistochemical stain was applied to sections from livers with a wide range of inflammatory lesions. Examination of sections from 53 dogs yielded five livers with small amounts of adenovirus. An additional virus-positive liver was identified from a dog with no hepatic inflammation. Although a cause and effect relationship remains to be determined, these findings suggest a possible connection between canine adenovirus and spontaneous chronic hepatitis.

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