scholarly journals Antiradical and Xanthine Oxidase Inhibitory Activity Evaluations of Averrhoa bilimbi L. Leaves and Tentative Identification of Bioactive Constituents through LC-QTOF-MS/MS and Molecular Docking Approach

Antioxidants ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 137 ◽  
Author(s):  
Qamar Ahmed ◽  
Alhassan Alhassan ◽  
Alfi Khatib ◽  
Syed Shah ◽  
Muhammad Hasan ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Bioactive Constituents ◽  
Butanol Fraction ◽  
Scavenging Effect ◽  
Radical Scavenging Effect ◽  
Dpph Radical Scavenging

The objective of the present study was to investigate the antiradical and xanthine oxidase inhibitory effects of Averrhoa bilimbi leaves. Hence, crude methanolic leaves extract and its resultant fractions, namely hexane, chloroform, and n-butanol were evaluated for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect and xanthine oxidase inhibitory activity. The active constituents were tentatively identified through LC-QTOF-MS/MS and molecular docking approaches. The n-butanol fraction of A. bilimbi crude methanolic leaves extract displayed significant DPPH radical scavenging effect with IC50 (4.14 ± 0.21 μg/mL) (p < 0.05), as well as xanthine oxidase inhibitory activity with IC50 (64.84 ± 3.93 μg/mL) (p < 0.05). Afzelechin 3-O-alpha-l-rhamnopyranoside and cucumerin A were tentatively identified as possible metabolites that contribute to the antioxidant activity of the n-butanol fraction.

scholarly journals Comparison of Biological Activities of Different Parts of Asparagus

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 771-771
Author(s):  
Jiseon Park ◽  
Jaegeel Lim
Keyword(s):  
Inhibitory Activity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Control Group ◽  
Total Phenol Content ◽  
No Production ◽  
Dpph Radical ◽  
Scavenging Effect ◽  
Radical Scavenging Effect ◽  
Different Parts

Abstract Objectives Asparagus is a spring vegetable, a flowering perennial plant species belonging to the lily family. Technavio's analysts forecast the Global Asparagus Market to grow at a CAGR of 3.15% during the period 2018–2022. All parts of asparagus, including roots, are rich in bioactive chemicals. However, other parts except asparagus spears are discarded. This study compared the physiological activity of different parts of asparagus and tried to improve the utilization of other parts. Methods Asparagus was divided into root, spear (5 cm from the bottom, 25 cm from the top, respectively), leaf and stem in order and used after lyophilized. Total phenol content, antioxidant activity (DPPH radical elimination ability, ABTS radical elimination ability), α-amylase inhibitory activity, and the effects of nitric oxide (NO) production were measured. Results The total polyphenol content of asparagus was highest in the leaves (22.87 mg TAE/g) and lowest in 5 cm under part of the spear(3.03 mg TAE/g). DPPH radical scavenging effect (44.52% in 1 mg/mL, 92.84% in 5 mg/mL), and ABTS radical scavenging effect(15.58% in 1 mg/mL, 48.02% in 5 mg/1 mL) were the highest on the leaf. On 5 cm under part of the spear, α-amylase inhibitory activity (79.16%) was the higher than any other parts. NO generation was significantly hindered by LPS treatment group 5 cm under part of the spear (12.93 μM), 25 cm above the part of the spear (12.10 μM), root (11.68 μM), leaf(10.43 μM), and stem(9.70 μM) in order(P &lt; 0.001). All the extracts were treated with the cells at concentrations of 100, 500 and 1000 µg/mL for one day. As a result, the survival rate of the macrophages was not significantly different from that of the control group. So it was confirmed that there is no cytotoxicity. Conclusions Thus, these results suggest that the another parts of asparagus may be highly valuable as a high-quality functional component as well as its-antioxidant, antiinflammatory activities. we believe that the results obtained would be helpful in the development of nutraceutics. Funding Sources This study was supported by 2019 Regional Specialized Technology Development Project, Rural Development Administration, Republic of Korea.

DPPH radical-scavenging effect on some constituents from the aerial parts of Lippia triphylla

2007 ◽  
Vol 62 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Masateru Ono ◽  
Eriko Oda ◽  
Takemi Tanaka ◽  
Yoshihiko Iida ◽  
Toru Yamasaki ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Dpph Radical ◽  
Scavenging Effect ◽  
Aerial Parts ◽  
Radical Scavenging Effect ◽  
Dpph Radical Scavenging ◽  
Lippia Triphylla

DPPH Radical-scavenging Effect of Several Phenylpropanoid Compounds and Their Glycoside Derivatives

2005 ◽  
Vol 125 (4) ◽  
pp. 371-375 ◽  
Author(s):  
Hitoshi TOMINAGA ◽  
Yuka KOBAYASHI ◽  
Takashi GOTO ◽  
Kazuo KASEMURA ◽  
Masato NOMURA
Keyword(s):  
Radical Scavenging ◽  
Dpph Radical ◽  
Scavenging Effect ◽  
Radical Scavenging Effect ◽  
Dpph Radical Scavenging

scholarly journals Phytochemicals in leaves of Cotoneaster mongolica, their antioxidative, and acetylcholinesterase inhibitory activity

2019 ◽  
Vol 20 (46) ◽  
pp. 1-6 ◽  
Author(s):  
Odontuya Gendaram
Keyword(s):  
Inhibitory Activity ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Butanol Fraction ◽  
Acetylcholinesterase Inhibitory Activity ◽  
Corosolic Acid ◽  
Ache Inhibitory Activity ◽  
Dpph Radical Scavenging ◽  
Flavonol Derivatives

The phytochemicals in the leaves of Cotoneaster mongolica Pojark, as well as their antioxidant and acetylcholinesterase (AChE) inhibitory activity were studied. The methanol extract of the leaves showed acetylcholinesterase inhibitory activity (IC50, 32.61 ± 0.51 μg/mL). The n-butanol fraction of this extract exhibited DPPH radical scavenging (IC50, 55.70 ± 0.15 μg/mL) and AChE inhibitory activity (IC50, 72.50 ± 0.60 μg/mL). From the n-butanol fraction quercetin (1), hyperoside (2), kaempferol-5-O-β-D-glucopyranoside (3), sissotrin (4), ursolic acid (5), corosolic acid (6), euscaphic acid (7), prunasin (8), (2R)-mandeloyl-β-D-glucopyranose (9), (Z)-3-hexenyl-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (10), benzyl-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (11) and arbutin (12) have been isolated and identified. Hyperoside, one of the major constituents among the isolated compounds, was active in both tested assays. Flavonol derivatives could provide the activity of this plant species.

scholarly journals Phytochemical, Antibacterial and Antioxidant Activity Evaluation of Rhodiola crenulata

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3664
Author(s):  
Lingyun Zhong ◽  
Lianxin Peng ◽  
Jia Fu ◽  
Liang Zou ◽  
Gang Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethyl Gallate ◽  
Dpph Radical ◽  
Rhodiola Crenulata ◽  
Dpph Radical Scavenging

The chemical components, as well as the antibacterial and antioxidant activities of the essential oil (EO) and crude extracts prepared from Rhodiola crenulata were investigated. The essential oil was separated by hydrodistillation, and gas chromatography-mass spectrometry (GC-MS) was used to identify its constituents. A total of twenty-seven compounds was identified from the EO, and its major components were 1-octanol (42.217%), geraniol (19.914%), and 6-methyl-5-hepten-2-ol (13.151%). Solvent extraction and fractionation were applied for preparing the ethanol extract (crude extract, CE), petroleum ether extract (PE), ethyl acetate extract (EE), n-butanol extract (BE), and water extract (WE). The CE, EE and BE were abundant in phenols and flavonoids, and EE had the highest total phenol and total flavonoid contents. Gallic acid, ethyl gallate, rosavin and herbacetin were identified in the EE. The antibacterial activity results showed that the EO exhibited moderate inhibitory activity to the typical clinic bacteria, and EE exhibited the strongest antibacterial activity among the five extracts. For the compounds, ethyl gallate showed the strongest inhibitory activity to the test bacteria, and its minimum inhibitory concentration (MIC) value and minimum bactericidal concentration (MBC) value for all the tested bacteria was 0.24 mg/mL and 0.48 mg/mL, respectively. The results of antioxidant activity showed that both CE and EE exhibited strong antioxidant activities in the DPPH radical scavenging and Fe2+ reducing power tests, however, EO showed relatively weaker antioxidant ability. Ethyl gallate and rosavin exhibited excellent activity in the DPPH radical scavenging assay, and their IC50 value was 5.3 µg/mL and 5.9 µg/mL, respectively. Rosavin showed better reduction power activity than the other three compounds. These results could provide more evidence for the traditional use of R. crenulata, and would be helpful for improving its application further.

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