scholarly journals Unraveling the Lipidome and Antioxidant Activity of Native Bifurcaria bifurcata and Invasive Sargassum muticum Seaweeds: A Lipid Perspective on How Systemic Intrusion May Present an Opportunity

Antioxidants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 642
Author(s):  
Fábio Santos ◽  
João P. Monteiro ◽  
Daniela Duarte ◽  
Tânia Melo ◽  
Diana Lopes ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Lipid Profile ◽  
Lipid Content ◽  
Polar Lipid ◽  
Sargassum Muticum ◽  
Brown Seaweeds ◽  
Bifurcaria Bifurcata ◽  
Chromatography Mass Spectrometry ◽  
Lipid Species

Brown seaweeds are known to present components with appealing bioactive properties eliciting great interest for industrial applications. However, their lipid content is generally disregarded beyond their fatty acid (FA) composition. This study thoroughly characterized the lipid profile of two brown seaweeds collected from Portuguese coast, the native Bifurcaria bifurcata and the invasive Sargassum muticum species, and bioprospecting for antioxidant activity. An integrated state-of-the-art approach including gas chromatography-mass spectrometry (GC–MS) and liquid chromatography-mass spectrometry (HILIC–ESI-MS/MS), allowed a comprehensive picture of FA and polar lipid content. Polar lipid profile of B. bifurcata and S. muticum included 143 and 217 lipid species respectively, distributed between glycolipids, phospholipids, and betaine lipids. Some of the lipid species found have been assigned biological activity and contain of n-3 and n-6 FA. Sargassum muticum presented the highest n-3 FA content. Low concentrations of extracts of both seaweeds displayed antioxidant activity, with S. muticum presenting more promising results. These findings contribute to the nutritional and industrial exploitation of both seaweeds, highlighting their relevance as viable sources of bioactive and added-value compounds. Sargassum muticum presented interesting lipid composition and bioactivity, which may represent an accessible opportunity for the exploitation of this invasive seaweed, especially taking advantage of Sargassum blooms.

Characterization of the lipid profile of Antarctic brown seaweeds and their endophytic fungi by gas chromatography–mass spectrometry (GC–MS)

Polar Biology ◽  
2019 ◽  
Vol 42 (8) ◽  
pp. 1431-1444 ◽  
Author(s):  
Thaiz Rodrigues Teixeira ◽  
Gustavo Souza Santos ◽  
Izabel Cristina Casanova Turatti ◽  
Mário Henrique Paziani ◽  
Márcia Regina von Zeska Kress ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Lipid Profile ◽  
Endophytic Fungi ◽  
Gas Chromatography Mass Spectrometry ◽  
Brown Seaweeds ◽  
Chromatography Mass Spectrometry

scholarly journals A New Look for the Red Macroalga Palmaria palmata: A Seafood with Polar Lipids Rich in EPA and with Antioxidant Properties

Marine Drugs ◽  
2019 ◽  
Vol 17 (9) ◽  
pp. 533 ◽  
Author(s):  
Lopes ◽  
Melo ◽  
Meneses ◽  
Abreu ◽  
Pereira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Eicosapentaenoic Acid ◽  
Lipid Content ◽  
Polar Lipid ◽  
Antioxidant Properties ◽  
Polar Lipids ◽  
Palmaria Palmata ◽  
Potential Health ◽  
Lipid Species ◽  
Red Macroalga

Palmaria palmata is an edible red macroalga widely used for human consumption and valued for its high protein value. Despite its low total lipid content, it is rich in eicosapentaenoic acid (EPA). This seaweed has been scarcely explored with regard to its lipid composition. The polar lipids of seaweeds are nowadays recognized as important phytochemicals contributing to their add value valorization and providing support for claims of potential health benefits. The present study aimed to disclose the polar lipid profile of P. palmata, farmed in an integrated multi-trophic aquaculture (IMTA) through modern lipidomic approaches using high-resolution LC-MS and MS/MS and to screen for the antioxidant properties of this red macroalga. A total of 143 molecular species of lipids were identified, belonging to several classes of polar lipids, such as glycolipids, phospholipids, and betaine lipids. It is noteworthy that the most abundant lipid species in each class were esterified with eicosapentaenoic acid (EPA), accounting for more than 50% of the lipid content. The polar lipid extract rich in EPA showed antioxidant activity with an inhibition concentration (IC) of IC30 = 171 ± 19.8 µg/mL for α,α-diphenyl-β-picrylhydrazyl radical (DPPH●) and IC50 = 26.2 ± 0.1 µg/mL for 2,20-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS●+). Overall, this study highlights that P. palmata farmed in an IMTA framework can be a sustainable source of beneficial lipids with antioxidant activity. Moreover, this red macroalga can be exploited for future applications as a source of lipids rich in EPA for food and feed, nutraceuticals, and cosmetics.

scholarly journals COMPOSITION OF PURIFIED ANTHOCYANIN ISOLATED FROM TEAK AND IT’S IN VITRO ANTIOXIDANT ACTIVITY

2017 ◽  
Vol 9 (9) ◽  
pp. 258 ◽  
Author(s):  
Greeshma Murukan ◽  
Murugan K.
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
Oxygen Radical ◽  
Sulphonic Acid ◽  
Oxygen Radical Absorbance Capacity ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Objective: The present study evaluates purification, characterization of anthocyanin from in vitro culture of teak and its antioxidant potential.Methods: Anthocyanin was extracted from in vitro culture, purified by using amber lite XAD column and fractionated by Liquid chromatography mass spectrometry (LC-MS/MS). Various antioxidant assays were carried such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), Oxygen radical absorbance capacity (ORAC), Nitric oxide (NO) and Hydrogen peroxide (H2O2).Results: Liquid chromatography mass spectrometry (LC-MS/MS) revealed the major fraction as cyanidin 3-(2-xylosyl-rutinoside) with unknown peaks. The amount of anthocyanin was 15.23 mg/g monomeric anthocyanin. Further, the potential antioxidant capacity of the teak anthocyanin was comparable to common vegetables and fruits. Similarly, high correlations of anthocyanin with antioxidant activity, such as oxygen radical absorbance capacity (ORAC), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) (r = 0.95, 0.93, and 0.80) were found.Conclusion: The high anthocyanins content and potential antioxidant activity suggests that teak anthocyanin may be applied in the food industry as a good source of natural pigments

Rosa rubiginosa and Fraxinus oxycarpa herbal teas: characterization of phytochemical profiles by liquid chromatography-mass spectrometry, and evaluation of the antioxidant activity

2017 ◽  
Vol 41 (15) ◽  
pp. 7681-7688 ◽  
Author(s):  
J. Jiménez-López ◽  
A. Ruiz-Medina ◽  
P. Ortega-Barrales ◽  
E. J. Llorent-Martínez
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Folk Medicine ◽  
Liquid Chromatography Mass Spectrometry ◽  
Rosa Rubiginosa ◽  
Chromatography Mass Spectrometry ◽  
Phytochemical Profiles

The consumption of infusions and herbal teas has always been an important part of folk medicine.

scholarly journals AKTIVITAS ANTIOKSIDAN DAN IDENTIFIKASI SENYAWA EKSTRAK JAMUR LINGZHI (Ganoderma lucidum) DENGAN LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS)

EKOLOGIA ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 65-72
Author(s):  
Farida Nuraeni ◽  
Septi Bernadetha Br Sembiring
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Time Variation ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Dpph Method

Lingzhi mushroom is widely used as an alternative treatment to reduce blood pressure and blood sugar levels, to maximize the potential of the Lingzhi fungus antioxidant tests are carried out. This study aims to determine the potential antioxidant activity of water extract and 70% ethanol extract of Lingzhi mushroom with maceration extraction time variation and identification of compounds with Liquid Chromatography Mass Spectrometry (LC-MS). This research begins with the determination of fresh lingzhi mushrooms then made simplicia and extracted by maceration with time variation with soaking time of 24 hours, 48 hours and 72 hours each with 2 solvents namely water and 70% ethanol. The extract was tested by phytochemistry followed by testing the antioxidant activity of Lingzhi mushroom extract (ganoderma lucidum) by DPPH method. Then the compounds were identified by Liquid Chromatography-Mass Spectrometry (LC-MS). Based on the results of research that maceration for 1 hour (1 day) Lingzhi mushroom with 70% ethanol extraction has the potential as an antioxidant with an IC50 value of 94.83 ppm. The results of identification with LC-MS in 70% ethanol extract as a compound that has potential as an antioxidant are Bisphenol M compounds, and 1- - [[2- (3,4-Dimethoxyphenyl) amino]} -3-methyl-2-octylpyrido [1 , 2-a] benzimidazole-4-carbonitrile.

Neuritogenic and Neuroprotective Activities of the Essential Oil From Rhizomes of Curcuma alismatifolia

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986421
Author(s):  
Sirote Kochaphum ◽  
Parinya Banyat ◽  
Sarin Tadtong ◽  
Vipaporn Sareedenchai ◽  
Sirivan Athikomkulchai
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Chemical Analysis ◽  
Essential Oil ◽  
Cell Viability ◽  
Neuroprotective Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Neuritogenic Activity

The essential oil from rhizomes of Curcuma alismatifolia Gagnep. was extracted by hydrodistillation. Chemical analysis by gas chromatography-mass spectrometry identified 12 compounds, of which the most prominent were xanthorrhizol (82.2%), ar-curcumene (6.5%), α-cedrene (1.8%), and β-bisabolol (1.1%). The essential oil at 1 ng/mL showed neuroprotective and neuritogenic activity on P19-derived neurons by significantly enhancing cell viability, length, and branching numbers of the cultured P19-derived neurons. In addition, xanthorrhizol, the major compound of the essential oil, at 10 ng/mL also exhibited the protection of P19-derived neurons. However, the mechanism of their neuroprotective activity was not correlated with their antioxidant activity.

scholarly journals Polar Lipids Composition, Antioxidant and Anti-Inflammatory Activities of the Atlantic Red Seaweed Grateloupia turuturu

Marine Drugs ◽  
2021 ◽  
Vol 19 (8) ◽  
pp. 414
Author(s):  
Elisabete da Costa ◽  
Tânia Melo ◽  
Mariana Reis ◽  
Pedro Domingues ◽  
Ricardo Calado ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Polar Lipid ◽  
Polar Lipids ◽  
Bioactive Molecules ◽  
Integrative Approach ◽  
Red Seaweed ◽  
Grateloupia Turuturu ◽  
Lipid Species ◽  
Potential Applications ◽  
Anti Inflammatory

Grateloupia turuturu Yamada, 1941, is a red seaweed widely used for food in Japan and Korea which was recorded on the Atlantic Coast of Europe about twenty years ago. This seaweed presents eicosapentaenoic acid (EPA) and other polyunsaturated fatty acids (PUFAs) in its lipid fraction, a feature that sparked the interest on its potential applications. In seaweeds, PUFAs are mostly esterified to polar lipids, emerging as healthy phytochemicals. However, to date, these biomolecules are still unknown for G. turuturu. The present work aimed to identify the polar lipid profile of G. turuturu, using modern lipidomics approaches based on high performance liquid chromatography coupled to high resolution mass spectrometry (LC–MS) and gas chromatography coupled to mass spectrometry (GC–MS). The health benefits of polar lipids were identified by health lipid indices and the assessment of antioxidant and anti-inflammatory activities. The polar lipids profile identified from G. turuturu included 205 lipid species distributed over glycolipids, phospholipids, betaine lipids and phosphosphingolipids, which featured a high number of lipid species with EPA and PUFAs. The nutritional value of G. turuturu has been shown by its protein content, fatty acyl composition and health lipid indices, thus confirming G. turuturu as an alternative source of protein and lipids. Some of the lipid species assigned were associated to biological activity, as polar lipid extracts showed antioxidant activity evidenced by free radical scavenging potential for the 2,2′-azino-bis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS●+) radical (IC50 ca. 130.4 μg mL−1) and for the 2,2-diphenyl-1-picrylhydrazyl (DPPH●) radical (IC25 ca. 129.1 μg mL−1) and anti-inflammatory activity by inhibition of the COX-2 enzyme (IC50 ca. 33 µg mL−1). Both antioxidant and anti-inflammatory activities were detected using a low concentration of extracts. This integrative approach contributes to increase the knowledge of G. turuturu as a species capable of providing nutrients and bioactive molecules with potential applications in the nutraceutical, pharmaceutical and cosmeceutical industries.

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