scholarly journals Influence of Ceramic Lumineers on Inflammatory Periodontal Parameters and Gingival Crevicular Fluid IL-6 and TNF-α Levels—A Clinical Trial

2021 ◽  
Vol 11 (6) ◽  
pp. 2829
Author(s):  
Ali Alrahlah ◽  
Manea Altwaim ◽  
Abdulaziz Alshuwaier ◽  
Malik Eldesouky ◽  
Khaled M. Alzahrani ◽  
...  
Keyword(s):  
Flow Rate ◽  
Gingival Crevicular Fluid ◽  
Statistical Significance ◽  
Periodontal Pocket ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Tnf Α ◽  
Crevicular Fluid

The aim was to evaluate the effect of ceramic lumineers on inflammatory periodontal parameters, gingival crevicular fluid (GCF) flow rate and cytokine profile. Patients were provided with lumineers using standardized technique including minimal to no preparation. Ceramic lumineers were etched with hydrofluoric acid and teeth with phosphoric acid followed by adhesive cementation. Periodontal parameters (Plaque index (PI), bleeding on probing (BOP), periodontal pocket depth (PPD), and clinical attachment loss (CAL)) were recorded at baseline and after 4, 12, and 24 weeks of lumineer cementation. Assessment of GCF flow rate and levels of IL-6 and TNF-α was made using enzyme linked immunosorbent assay (ELISA). The statistical significance was determined by the t-test, analysis of variance and post hoc Tukey’s test. It was found that PI, BOP, PPD, and CAL at baseline and 24 weeks were comparable (p > 0.05). The GCF volume at baseline was comparable to the GCF at week 24 (p > 0.05). The IL-6 levels at baseline (5.4 ± 3.6) were similar to those at 24 week (7.4 ± 5.2) (p > 0.05). The TNF-α at week 4 (65.3 ± 16.2), 12 (25 ± 10.2), and 24 (21.3 ± 7.6) was higher than the baseline (13.7 ± 5.8) (p < 0.05). Clinical periodontal parameters and GCF volume among patients treated with ceramic lumineers at baseline and twenty-four week follow-up were comparable. The GCF TNF-α levels significantly increased after ceramic lumineer cementation at 24-week follow-up.

scholarly journals Effects of Initial Periodontal Therapy on Heat Shock Protein 70 Levels in Gingival Crevicular Fluid from Periodontitis Patients

2020 ◽  
Vol 9 (10) ◽  
pp. 3072
Author(s):  
Nobuhisa Furuse ◽  
Hideki Takai ◽  
Yorimasa Ogata
Keyword(s):  
Heat Shock ◽  
Gingival Crevicular Fluid ◽  
Periodontal Therapy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microbial Infection ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Factor Α ◽  
Crevicular Fluid ◽  
Shock Proteins

Periodontitis is an inflammatory disease of periodontium which is caused by periodontopathic bacteria. Moreover, various cytokines such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and IL-6 are expressed in the inflamed periodontium. Heat shock proteins (HSPs) protect cells from abnormal conditions including inflammation, microbial infection and diseases. The 70-kDa HSPs (HSP70s) are major HSPs that express in the inflamed tissues. In this study, an enzyme-linked immunosorbent assay was applied to measure the levels of HSP70 in gingival crevicular fluid (GCF) from two periodontal pockets in each of 10 patients with Stage III, Grade B periodontitis. Sites with probing pocket depth (PPD) of ≤3 mm were named the healthy control (HC) sites, and sites with PPD of ≥5 mm were named the diseased sites. HSP70 levels in GCF were expressed higher at diseased sites than at HC sites, and decreased after initial periodontal therapy at diseased sites. These results suggest the association of HSP70 with the stage of periodontitis.

scholarly journals S100A8 and S100A9 in saliva, blood and gingival crevicular fluid for screening periodontitis: a cross-sectional study

2020 ◽  
Author(s):  
Hyun-Duck Kim ◽  
Sandeep Karna ◽  
YooJin Shin ◽  
Huong Vu ◽  
Hyun-Jae Cho ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Characteristic Curve ◽  
Analysis Of Covariance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Oral Diseases ◽  
Early Screening ◽  
Clinical Attachment Loss ◽  
Cross Sectional ◽  
Crevicular Fluid ◽  
Healthy Participants

Abstract Background: Periodontitis is one of major oral diseases, which has no consensus on early screening tool. This study aimed to compare the association and screening ability of S100A8 and S100A9 in saliva, blood and gingival crevicular fluid (GCF) for periodontitis.Methods: We recruited 149 community Koreans, 50 healthy and 99 periodontitis. Using clinical attachment loss and a panoramic radiograph, stage II-IV of new classification of periodontitis proposed at 2018 was considered as periodontitis. Enzyme linked immunosorbent assay kit was used to quantify S100A8 and S100A9. T-test, analysis of covariance (ANCOVA), Mann-Whitney test and correlation analysis were applied to compare the relationship of S100A8 and S100A9 in saliva, blood, and GCF for periodontitis. Receiver operating characteristic curve was applied for screening ability.Results: Among S100A8 and S100A9 in saliva, blood and GCF, S100A8 in saliva was significantly higher in periodontitis participants than in healthy participants (p<0.05) and showed highest screening ability of 0.73 for periodontitis. However, S100A8 and S100A9 in GCF were significantly higher in healthy participants (p<0.05). Salivary S100A8 was positively correlated to blood S100A8 (r=0.21, p <0.05).Conclusion: Salivary S100A8 could be a potential diagnostic marker for periodontitis. Thus, S100A8 salivary kit will be useful for screening periodontitis.

scholarly journals GCF Adrenomedullin Levels in Healthy and Periodontitis Patients with or without Type 2—Diabetes Mellitus: Clinicobiochemical Study

2014 ◽  
Vol 5 (1) ◽  
pp. 42-46
Author(s):  
SM Apoorva ◽  
Divya Bhat ◽  
Akanksha Garg ◽  
A Suchetha ◽  
N Sapna ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Gingival Crevicular Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Crevicular Fluid

ABSTRACT Background The literature suggests that periodontal disease and diabetes mellitus share a two-way relationship. The aim of this study was to evaluate and compare the levels of adrenomedullin (ADM) in gingival crevicular fluid (GCF) of periodontally healthy and periodontitis patients with or without type 2 diabetes with different glycemic controls. Methods Ninety patients were included in the study and were divided into five groups based on CPI scores and ADA classification of diabetes. Probing pocket depth (PPD) and clinical attachment level (CAL) were measured in all the subjects. GCF was collected from all the participants using micropipettes. ADM levels were measured in GCF samples by enzyme-linked immunosorbent assay. Results The results showed higher levels of ADM in patients with periodontitis as compared to healthy group. Significant correlation was present between PPD and CAL and ADM levels in all periodontitis patients with or without type 2 diabetes. Conclusion Increase in GCF levels of ADM from periodontal health to disease and in periodontitis patients with type 2 diabetes with the worsening of glycemic control underlines the possible role of ADM in mounting a protective response to worsening disease state. How to cite this article Garg A, Suchetha A, Sapna N, Apoorva SM, Bhat D, Puzhankara L. GCF Adrenomedullin Levels in Healthy and Periodontitis Patients with or without Type 2—Diabetes Mellitus: Clinicobiochemical Study. World J Dent 2014;5(1):42-46.

scholarly journals Comparison of Proinflammatory Cytokine Levels in Gingival Crevicular Fluid around Dental Implants with Ceramic and Titanium Abutments

2017 ◽  
Vol 18 (9) ◽  
pp. 831-836
Author(s):  
Ramin Negahdari ◽  
Mahdi Rahbar ◽  
Vahid Fakhrzadeh ◽  
Hosein Eslami ◽  
Taleb Akbari ◽  
...  
Keyword(s):  
Dental Implants ◽  
Proinflammatory Cytokine ◽  
Gingival Crevicular Fluid ◽  
Statistical Significance ◽  
Titanium Implants ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cytokine Levels ◽  
Biologic Response ◽  
Ceramic Implants ◽  
Crevicular Fluid

ABSTRACT Background Considering the high success rate of osseointegration, there is ever-increasing use of dental implants. The mechanisms and biologic response of peri-implant tissues are different depending on the biocompatibility of the implant material. The aim of this study was to compare the proinflammatory cytokine levels in the gingival crevicular fluid (GCF) around dental implants with ceramic and titanium abutments. Materials and methods All the patients with dental implants referring to two private offices of two prosthodontists from June to August 2016 were examined in relation to implant health, and eligible subjects, based on inclusion criteria, were included in this study with a slit-mouth design. In this context, on one side titanium implants and, on the other side, ceramic implants were used. Samples were collected from the peri-implant sulcus in each patient and sent to the immunology laboratory for determination of interleukin (IL)-6 and IL-1β proinflammatory cytokine levels using an enzyme-linked immunosorbent assay kit. Data were analyzed with Statistical Package for the Social Sciences version 16. Statistical significance was set at p < 0.05. Results The results showed lower levels of IL-1β and IL-6 in the GCF around ceramic abutments compared with titanium implants. In addition, statistical comparison between IL-6 and IL-1β levels showed higher levels of IL-6 around titanium and ceramic abutments compared with IL-1β levels. Conclusion Based on the results of this study, the human body immune response to ceramic abutments is much better than that to titanium implants. Clinical significance Considering that proinflammatory cytokine levels in GCF around ceramic abutments are lower than titanium ones, ceramic abutments are preferred for clinical usage. How to cite this article Negahdari R, Rahbar M, Fakhrzadeh V, Eslami H, Akbari T, Bohluli S. Comparison of Proinflammatory Cytokine Levels in Gingival Crevicular Fluid around Dental Implants with Ceramic and Titanium Abutments. J Contemp Dent Pract 2017;18(9):831-836.

Periodontal status and salivary flow rate among diagnostic radiology department workers

2019 ◽  
Vol 31 (3) ◽  
pp. 17-20
Author(s):  
Ghadah N. Alhusaini ◽  
Ahlam T Mohammed
Keyword(s):  
Flow Rate ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Diagnostic Radiology ◽  
Periodontal Pocket ◽  
Control Group ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Periodontal Status ◽  
Significant Difference

Background: diagnostic radiology field workers are at elevated risk level for systemic and oral diseases like periodontal diseases. This study was aimed to estimate the periodontal condition and salivary flow rate among diagnostic radiology workers. Material and method: The sample for this study consisted of a study group radiographers (forty subjects) working for 5 years at least and control group consisted of nurses and laboratory workers away from radiation (forty subjects) in Baghdad hospitals. All the 80 subjects aged 30-40 year-old and looking healthy without systemic diseases. Plaque, gingival, periodontal pocket depth and clinical attachment loss indices were used for recording the periodontal conditions. Under standardized conditions, collection of unstimulated salivary samples was done and salivary flow rate was measured. Results: Although not significant statistically (p>0.01), analysis of the present study data showed that plaque and gingival indices were higher among radiographers. While periodontal pocket depth and clinical attachment loss were higher among radiographers than control group with statistically highly significant difference (p<0.01). On the other hand salivary flow rate was lower among radiographers than control group with statistically highly significant difference (p<0.01). Conclusions: Ionizing radiation affects salivary flow rate and this in turn will affect periodontal status.

scholarly journals Effectiveness of Probiotic Lozenges in Periodontal Management of Chronic Periodontitis Patients: Clinical and Immunological Study

2020 ◽  
Vol 14 (02) ◽  
pp. 281-287
Author(s):  
Ahmed Alshareef ◽  
Alaa Attia ◽  
Mohammed Almalki ◽  
Faisal Alsharif ◽  
Ahmed Melibari ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Large Scale ◽  
Gingival Crevicular Fluid ◽  
Immunological Study ◽  
Scaling And Root Planing ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Recall Time ◽  
Scale Population ◽  
Crevicular Fluid

Abstract Objective The main purpose of this article was to evaluate the effect of probiotics used as an adjunctive to scaling and root planing (SRP) on the periodontal parameters and matrix metalloproteinase-8 (MMP-8) levels in gingival crevicular fluid (GCF) of chronic periodontitis patients. Materials and Methods A total of 25 chronic periodontitis patients who completed the treatment course of 40 subjects, aged 25 to 58 years, participated in this study. They were categorized into two groups: the first group was treated by SRP while the second group was treated by SRP and probiotic lozenges twice a day for 30 days. All patients were evaluated clinically by measuring the plaque index, bleeding index (BI), pocket depth, clinical attachment loss, and immunologically by assaying GCF/MMP-8 at baseline and 30 days after periodontal management. Results There was a significant improvement in periodontal parameters after SRP treatment with and without probiotic lozenges in both groups. However, there was a significant decrease in the BI (p = 0.05) in SRP and probiotic lozenges group after 30 days compared with SRP alone. In addition, there was a significant decrease in GCF/MMP-8 levels after 30 days in patients managed by SRP only (p = 0.017) compared with the baseline in both groups, whereas a highly significant decrease in patients treated by SRP and probiotics (p = 0.001). Conclusion The current study suggested that the probiotics might have a beneficial effect on clinical and immunological outcomes in the management of chronic periodontitis patients. Further research is needed on a large-scale population and for a long recall time to confirm the response to probiotics as an adjunctive to SRP.

scholarly journals Interleukin-1 receptor antagonist levels in gingival crevicular fluid and serum in nonsmoking women with preterm low birth weight and intrauterine growth retardation

2015 ◽  
Vol 09 (01) ◽  
pp. 109-116 ◽  
Author(s):  
Nezahat Arzu Kayar ◽  
Nilgun Ozlem Alptekin ◽  
Seyfullah Haliloglu
Keyword(s):  
Birth Weight ◽  
Low Birth Weight ◽  
Receptor Antagonist ◽  
Growth Retardation ◽  
Gingival Crevicular Fluid ◽  
Interleukin 1 ◽  
Serum Samples ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Crevicular Fluid

ABSTRACT Objective: The aim of this study was to evaluate interleukin (IL)-1 β and IL-1 receptor antagonist (IL-1ra) levels in gingival crevicular fluid (GCF) and serum (S) in nonsmoking women with normal birth (NB), preterm low birth weight (PLBW), and intra-uterine growth retardation (IUGR). Materials and Methods: In this unmatched case-control study design, 64 women with NB, 45 women with PLBW, and 47 women with IUGR were recruited within 24 h delivery. Clinical periodontal parameters were recorded. IL-1 β and IL-1ra levels in GCF (pg/30 s) and serum (pg/ml) of were evaluated using commercial enzyme immunoassay and ELISA kits. Results: Greater pocket depth and clinical attachment loss were observed in PLBW and IUGR women than in NB women (P < 0.05). The total amounts of IL-1ra and IL-β of GCF were higher levels in NB women than PLBW and IUGR women (P < 0.05). The lowest total amount of IL-1ra of GCF was found in IUGR women (P < 0.05). The concentrations of IL-1ra in serum samples were not statistically significant for any of the study groups (P > 0.05). Conclusion: It can be suggested that worse periodontal conditions and the low levels of IL-1ra in GCF may be an important factor in adverse pregnancy outcomes.

scholarly journals PROSTAGLANDIN LEVELS IN GINGIVAL CREVICULAR FLUID IN PERIODONTITIS PATIENT WITH HYPERTENSION

2020 ◽  
pp. 19-22
Author(s):  
RADITYA PRIHARNANTO ◽  
ROBERT LESSANG ◽  
SRI LELYATI C MASULILI ◽  
FATIMAH MARIA TADJOEDIN ◽  
HERLIS RAHDEWATI ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Periodontal Disease ◽  
Prostaglandin E2 ◽  
Gingival Crevicular Fluid ◽  
Clinical Sample ◽  
Tooth Surface ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Significant Difference ◽  
Crevicular Fluid

Objective: Periodontitis is an infection that involved tooth-supporting tissues by dental plaque biofilm on the tooth surface and host immune responseas causal to as inflammation resolution. Prostaglandin E2 (PGE2) as an inflammatory mediator has been implicated in the pathogenesis of variouschronic inflammatory diseases, including periodontitis, and as a regulator of blood pressure. This study aims to compare the levels of prostaglandinin the gingival crevicular fluid (GCF) of periodontitis patients with hypertension and non-hypertension.Methods: A total sample of 62 patients was examined and consists of 44 patients (aged 50–90 years) who were hypertension group and 18 patients(aged 30–49 years) who were non-hypertension groups as a control. Hypertension state was measured by blood pressure based on anamnesisand using sphygmomanometer mercury. The clinical sample was collected from 62 gingival crevicular fluids [GCF] of periodontal disease subject.Measurement of the clinical parameter of probing pocket depth [PD], and bleeding on probing [BOP ≥1] was included as a diagnostic requisition.The PD and clinical attachment loss [CAL] was defined as present if the PD was ≥5 mm and CAL was ≥1 mm. Prostaglandin E2 level was estimated ingingival crevicular fluid samples by using the enzyme-linked immunosorbent assay.Results: The level of PGE2 was statistically significant difference in hypertension patient compare with non-hypertension (p<0.05). There was asignificant difference in PD, recession, and CAL (p<0.05).Conclusion: Higher blood pressure might be related to the potential risk of inflammation and progression periodontal disease.

scholarly journals The Effect of Periodontal and Peri-Implanter Health on IL-1β and TNF-α Levels in Gingival Crevicular and Peri-Implanter Sulcus Fluid: a Cross-Sectional Study

2020 ◽  
pp. 346-355
Author(s):  
Hasan Gündoğar ◽  
Meral Uzunkaya
Keyword(s):  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Cross Sectional Study ◽  
Periodontal Pocket ◽  
Marginal Bone Loss ◽  
Pocket Depth ◽  
Cross Sectional ◽  
Tnf Α ◽  
Significant Difference ◽  
Program Sample

It is stated that peri-implanter diseases have destructive effects similar to periodontal diseases. This study aims to compare IL-1β and TNF-α levels in healthy and diseased areas. Forty non-smokers systemically health individuals (40 implants/40 teeth) (age:38-67) were included in the study. In clinical and radiographic examinations; plaque index (PIn), gingival index (GI), periodontal pocket depth (PPD), clinical attachment level (CAL) and marginal bone loss (MBL) values were recorded. The gingival crevicular fluid (GCF) and peri-implanter sulcus fluids (PISF) of the patients were collected, and IL-1β and TNF-α levels were measured by ELISA in the samples. The collected data were analyzed with the help of SPSS v.22 package program. Sample PPD score showed a statistically significant difference between the diseased and healthy groups and also sample CAL showed statistically significant higher in Periodontitis(P) compared to periodontally heathy(H) and Gingivitis(G) (p>0.05). IL-1beta and TNF-α levels in GCF/PISF; In the P and Peri-implantitis (PI) group, it was found statistically significantly higher than the H, Healthy-Implant (HI), besides P showed higher levels compared to G (p<0.05). Within the limitations of our study, it can be said that IL-1β and TNF-α among inflammatory cytokines in GCF/PISF will increase in periodontal and peri-implanter diseases, it can also be said that this increase in cytokines may indicate that periodontal and peri-implanter diseases have similar immunological structure. Peri-implant mucositis without periodontitis history has similarity to peri-implantitis and periodontitis in terms of IL-1β and TNF-α levels in GCF/PISF.

Sign in / Sign up

Export Citation Format

Share Document