A Biomimetic Polynucleotides–Hyaluronic Acid Hydrogel Promotes Wound Healing in a Primary Gingival Fibroblast Model

Polynucleotides (PN) have long been known as an effective supportive therapy for wound healing. The present study investigated whether a hydrogel formulation containing PN and hyaluronic acid (PN + HA) could promote wound healing in an in vitro model of gingival fibroblasts. PN promoted cell growth and viability as assessed by different assays, and PN + HA, though not significantly further increasing cell growth as compared to PN, supported the formation of dense multilayered cell nodules. PN promoted fibroblasts’ clonogenic efficiency and PN + HA further enhanced the formation of more numerous dense colonies. PN + HA appeared to significantly increase the expression of collagen 1a1 and 3a1, while not affecting proteoglycans deposition. Interestingly, when tested in a scratch assay, PN + HA achieved gap closure after 48 h, while cells in the comparison groups had not completely bridged the scratch even after 96 h. Taken together, these results demonstrate that PN + HA is a promising candidate for a supportive therapy to promote soft tissue healing in the oral cavity.

Download Full-text

An in vitro model for investigation of vitamin A effects on wound healing

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000692 ◽

2021 ◽

pp. 1-6

Author(s):

Hoda Keshmiri Neghab ◽

Mohammad Hasan Soheilifar ◽

Gholamreza Esmaeeli Djavid

Keyword(s):

Wound Healing ◽

Endothelial Cell ◽

Vitamin A ◽

In Vitro Model ◽

Cellular Proliferation ◽

Endothelial Cell Migration ◽

Normal Fibroblast ◽

Anti Inflammatory ◽

Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inﬂammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inﬂammation responses.

Download Full-text

The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination

Communications Biology ◽

10.1038/s42003-021-01774-2 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Giovanni S. Offeddu ◽

Cynthia Hajal ◽

Colleen R. Foley ◽

Zhengpeng Wan ◽

Lina Ibrahim ◽

...

Keyword(s):

Hyaluronic Acid ◽

Tumor Cells ◽

Cancer Progression ◽

Vascular System ◽

Migration And Invasion ◽

Improve Patient Survival ◽

Adhesive Interactions ◽

Vitro Model ◽

Metastatic Sites

AbstractThe glycocalyx on tumor cells has been recently identified as an important driver for cancer progression, possibly providing critical opportunities for treatment. Metastasis, in particular, is often the limiting step in the survival to cancer, yet our understanding of how tumor cells escape the vascular system to initiate metastatic sites remains limited. Using an in vitro model of the human microvasculature, we assess here the importance of the tumor and vascular glycocalyces during tumor cell extravasation. Through selective manipulation of individual components of the glycocalyx, we reveal a mechanism whereby tumor cells prepare an adhesive vascular niche by depositing components of the glycocalyx along the endothelium. Accumulated hyaluronic acid shed by tumor cells subsequently mediates adhesion to the endothelium via the glycoprotein CD44. Trans-endothelial migration and invasion into the stroma occurs through binding of the isoform CD44v to components of the sub-endothelial extra-cellular matrix. Targeting of the hyaluronic acid-CD44 glycocalyx complex results in significant reduction in the extravasation of tumor cells. These studies provide evidence of tumor cells repurposing the glycocalyx to promote adhesive interactions leading to cancer progression. Such glycocalyx-mediated mechanisms may be therapeutically targeted to hinder metastasis and improve patient survival.

Download Full-text

Evaluation of characteristics and in vitro antioxidant properties of RSV loaded hyaluronic acid–DPPC microparticles as a wound healing system

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2014.12.006 ◽

2015 ◽

Vol 126 ◽

pp. 50-57 ◽

Cited By ~ 26

Author(s):

İpek Eroğlu ◽

Evren H. Gökçe ◽

Nicolas Tsapis ◽

Sakine Tuncay Tanrıverdi ◽

Göksel Gökçe ◽

...

Keyword(s):

Wound Healing ◽

Hyaluronic Acid ◽

Antioxidant Properties ◽

Healing System

Download Full-text

Fetal Bovine Serum-Derived Extracellular Vesicles Persist within Vesicle-Depleted Culture Media

International Journal of Molecular Sciences ◽

10.3390/ijms19113538 ◽

2018 ◽

Vol 19 (11) ◽

pp. 3538 ◽

Cited By ~ 20

Author(s):

Brandon Lehrich ◽

Yaxuan Liang ◽

Pooya Khosravi ◽

Howard Federoff ◽

Massimo Fiandaca

Keyword(s):

Cell Growth ◽

Extracellular Vesicles ◽

Bovine Serum ◽

Culture Media ◽

Fetal Bovine Serum ◽

Cellular Growth ◽

Primary Astrocyte ◽

Fetal Bovine ◽

Cell Growth And Viability

It is known that culture media (CM) promotes cellular growth, adhesion, and protects explanted primary brain cells from in vitro stresses. The fetal bovine serum (FBS) supplement used in most CM, however, contains significant quantities of extracellular vesicles (EVs) that confound quantitative and qualitative analyses from the EVs produced by the cultured cells. We quantitatively tested the ability of common FBS EV-depletion protocols to remove exogenous EVs from FBS-supplemented CM and evaluated the influence such methods have on primary astrocyte culture growth and viability. We assessed two methodologies utilized for FBS EV removal prior to adding to CM: (1) an 18-h ultracentrifugation (UC); and (2) a commercial EV-depleted FBS (Exo-FBS™). Our analysis demonstrated that Exo-FBS™ CM provided the largest depletion (75%) of total FBS EVs, while still providing 6.92 × 109 ± 1.39 × 108 EVs/mL. In addition, both UC and Exo-FBS™ CM resulted in poor primary astrocyte cell growth and viability in culture. The two common FBS EV-depletion methods investigated, therefore, not only contaminate in vitro primary cell-derived EV analyses, but also provide a suboptimal environment for primary astrocyte cell growth and viability. It appears likely that future CM optimization, using a serum-free alternative, might be required to advance analyses of cell-specific EVs isolated in vitro.

Download Full-text

099 "Smart" Targeting of Anti?Scarring Agents in an in vitro Model of Retinal Wound Healing

Wound Repair and Regeneration ◽

10.1111/j.1067-1927.2004.0abstractcs.x ◽

2004 ◽

Vol 12 (2) ◽

pp. A26-A26

Author(s):

F E Dhawahir ◽

C Sheridan ◽

D Kent ◽

D Wong ◽

I Grierson ◽

...

Keyword(s):

Wound Healing ◽

In Vitro Model ◽

Vitro Model

Download Full-text

A functional in vitro model to examine signaling mechanisms in gastrin-mediated human cell growth,

Journal of Gastrointestinal Surgery ◽

10.1016/s1091-255x(97)80012-1 ◽

1997 ◽

Vol 1 (1) ◽

pp. 69-77 ◽

Cited By ~ 1

Author(s):

H Kim

Keyword(s):

Cell Growth ◽

Human Cell ◽

In Vitro Model ◽

Signaling Mechanisms ◽

Vitro Model

Download Full-text

Enhancing the biocompatibility of siliconepolycarbonate urethane based implant materials

Current Directions in Biomedical Engineering ◽

10.1515/cdbme-2019-0114 ◽

2019 ◽

Vol 5 (1) ◽

pp. 453-455 ◽

Cited By ~ 1

Author(s):

Kiriaki Athanasopulu ◽

Larysa Kutuzova ◽

Joana Thiel ◽

Günter Lorenz ◽

Ralf Kemkemer

Keyword(s):

Molecular Weight ◽

Cell Proliferation ◽

Hyaluronic Acid ◽

Cell Growth ◽

Block Copolymers ◽

Cytotoxic Potential ◽

Implant Surface ◽

Characterization Methods ◽

Hard Segments

AbstractPolyurethane-bases block copolymers (TPCUs) are block-copolymers with systematically varied soft and hard segments. They have been suggested to serve as material for chondral implants in joint regeneration. Such applications may require the adhesion of chondrocytes to the implant surface, facilitating cell growth while keeping their phenotype. Thus, aims of this work were (i) to modify the surface of soft biostable polyurethane-based model implants (TPCU and TSiPCU) with high-molecular weight hyaluronic acid (HA) using an optimized multistep strategy of immobilization, and (ii) to evaluate bioactivity of the modified TPCUs in vitro. Our results show no cytotoxic potential of the TPCUs. HAbioactive molecules (Mw =700kDa) were immobilized onto the polyurethane surface via polyethylenimine (PEI) spacers, and modifications were confirmed by several characterization methods. Tests with porcine chondrocytes indicated the potential of the TPCU-HA for inducing enhanced cell proliferation.

Download Full-text