scholarly journals Biovalorization of Lignocellulosic Materials for Xylitol Production by the Yeast Komagataella pastoris

2021 ◽  
Vol 11 (12) ◽  
pp. 5516
Author(s):  
Diana Araújo ◽  
Tatiana Costa ◽  
Filomena Freitas
Keyword(s):  
Cell Growth ◽  
Grape Pomace ◽  
Lignocellulosic Materials ◽  
Xylitol Production ◽  
Market Demand ◽  
Lignocellulosic Waste ◽  
Dilute Acid Hydrolysis ◽  
Fourfold Increase ◽  
Grape Stalks ◽  
Komagataella Pastoris

The main goal of this study was to screen different lignocellulosic materials for their ability to support the cell growth of the yeast Komagataella pastoris and the production of xylitol. Several lignocellulosic materials, namely banana peels, brewer’s spent grains (BSGs), corncobs, grape pomace, grape stalks, and sawdust, were subjected to dilute acid hydrolysis to obtain sugar rich solutions that were tested as feedstocks for the cultivation of K. pastoris. Although the culture was able to grow in all the tested hydrolysates, a higher biomass concentration was obtained for banana peels (15.18 ± 0.33 g/L) and grape stalks (14.58 ± 0.19 g/L), while the highest xylitol production (1.51 ± 0.07 g/L) was reached for the BSG hydrolysate with a xylitol yield of 0.66 ± 0.39 g/g. Cell growth and xylitol production from BSG were improved by detoxifying the hydrolysate using activated charcoal, resulting in a fourfold increase of the biomass production, while xylitol production was improved to 3.97 ± 0.10 g/L. Moreover, concomitant with arabinose consumption, arabitol synthesis was noticed, reaching a maximum concentration of 0.82 ± 0.05 g/L with a yield on arabinose of 0.60 ± 0.11 g/g. These results demonstrate the feasibility of using lignocellulosic waste, especially BSG, as feedstock for the cultivation of K. pastoris and the coproduction of xylitol and arabitol. Additionally, it demonstrates the use of K. pastoris as a suitable microorganism to integrate a zero-waste biorefinery, transforming lignocellulosic waste into two high-value specialty chemicals with high market demand.

scholarly journals Bioconversion of hydrolyzed cashew peduncle bagasse for ethanol and xylitol production

2017 ◽  
Vol 21 (7) ◽  
pp. 488-492 ◽  
Author(s):  
Lorena L. de Medeiros ◽  
Flávio L. H. da Silva ◽  
Sharline F. M. Santos ◽  
Marta S. Madruga ◽  
Débora J. N. de Melo ◽  
...  
Keyword(s):  
Cell Growth ◽  
Industrial Waste ◽  
Large Scale ◽  
Fermentation Process ◽  
Candida Guilliermondii ◽  
Anacardium Occidentale ◽  
Xylitol Production ◽  
Maximum Production ◽  
Maximum Cell ◽  
Scale 8

ABSTRACT The agro-industrial waste deposited in the environment causes problems in nature that can be solved with the use and generation of bioproducts. Thus, the objective was to study the lignocellulosic fraction of cashew (Anacardium occidentale L.) peduncle bagasse and fermentation on large scale (8-16 times) using the strain Candida guilliermondii CCT-3544 as production agent. According to the obtained results, it can be noted that the dry cashew peduncle bagasse has 21.45% of cellulose, 10.96% of hemicellulose and 35.39% of lignin. During fermentation, C. guilliermondii 3544-CAT was able to grow on medium containing hydrolysate, with maximum cell growth concentration of 3.5 g L-1. The behavior of the sugars in the fermentation process was similar in the different variables, with maximum production of ethanol and xylitol at 48 h of fermentation.

scholarly journals Xylose recovery from dilute-acid hydrolysis of oil palm (Elaeis guineensis) empty fruit bunches for xylitol production

2017 ◽  
Vol 16 (41) ◽  
pp. 1997-2008
Author(s):  
Manjarres-Pinzon Katherine ◽  
Arias-Zabala Mario ◽  
Correa-Londono Guillermo ◽  
Rodriguez-Sandoval Eduardo
Keyword(s):  
Acid Hydrolysis ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Xylitol Production ◽  
Dilute Acid ◽  
Dilute Acid Hydrolysis ◽  
Empty Fruit Bunches ◽  
Hydrolysis Of

scholarly journals Adenovirus E1A-Regulated Transcription Factor p120E4F Inhibits Cell Growth and Induces the Stabilization of the cdk Inhibitor p21WAF1

1998 ◽  
Vol 18 (1) ◽  
pp. 459-467 ◽  
Author(s):  
Elma R. Fernandes ◽  
Jun Yuan Zhang ◽  
Robert J. Rooney
Keyword(s):  
Transcription Factor ◽  
Cell Growth ◽  
Cell Cycle Progression ◽  
Kinase Activity ◽  
Fibroblast Cell ◽  
Binding Activity ◽  
Cdk Inhibitor ◽  
Cyclin D ◽  
Fourfold Increase ◽  
Adenovirus E1a

ABSTRACT Adenovirus E1A proteins influence cell growth and phenotype through physical interactions with cellular proteins that regulate basic processes such as cell cycle progression, DNA synthesis, and differentiation. p120E4F is a low-abundance cellular transcription factor that represses the adenovirus E4 promoter and is regulated by E1A, through a phosphorylation-induced reduction of its DNA binding activity, to permit activation of the E4 promoter during early infection. To determine the normal biological role of p120E4F, we assessed its ability to influence fibroblast cell growth and transformation. p120E4F suppressed NIH 3T3 fibroblast colony formation but had little effect when coexpressed with E1A and/or activated ras. Cells that overexpressed p120E4F were inhibited in their ability to enter S phase, had elevated levels of the cdk inhibitor p21 WAF1 , and reduced cyclin D-cdk4/6 kinase activity. The increase of p21 WAF1 levels occurred through a p53-independent posttranscriptional mechanism that included a three- to fourfold increase in the half-life of p21 WAF1 protein. Coexpression of activatedras with p120E4F stimulated cyclin D1 expression, elevated cyclin D-cdk4/6 kinase activity, and accelerated cell growth. These data suggest an important role for p120E4F in normal cell division and demonstrate that p21 WAF1 can be regulated by protein turnover.

Xylitol production from dilute-acid hydrolysis of bean group shells

2010 ◽  
Author(s):  
Chizuru Sasaki ◽  
Akihiro Kurosumi ◽  
Yuya Yamashita ◽  
Godliving Mtui ◽  
Yoshitoshi Nakamura
Keyword(s):  
Acid Hydrolysis ◽  
Xylitol Production ◽  
Dilute Acid ◽  
Dilute Acid Hydrolysis ◽  
Hydrolysis Of

Influence of Oxygen Availability on Cell Growth and Xylitol Production by Candida guilliermondii

2002 ◽  
Vol 98-100 (1-9) ◽  
pp. 449-458 ◽  
Author(s):  
Luis F. Figueiredo Faria ◽  
Maria Antonieta P. Gimenes ◽  
Ronaldo Nobrega ◽  
Nei Pereira, Jr.
Keyword(s):  
Cell Growth ◽  
Candida Guilliermondii ◽  
Oxygen Availability ◽  
Xylitol Production

scholarly journals Bioactive Compounds from Vine Shoots, Grape Stalks, and Wine Lees: Their Potential Use in Agro-Food Chains

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 342
Author(s):  
Marica Troilo ◽  
Graziana Difonzo ◽  
Vito M. Paradiso ◽  
Carmine Summo ◽  
Francesco Caponio
Keyword(s):  
Bioactive Compounds ◽  
Food Chain ◽  
Food Packaging ◽  
Global Market ◽  
Grape Pomace ◽  
Food Sector ◽  
Sustainable Food ◽  
Functional Additives ◽  
By Products ◽  
Grape Stalks

The winemaking sector is one of the most productive worldwide, and thus it also generates large amounts of by-products with high environmental impacts. Furthermore, global market trends and government regulations promote industrial alternatives based on sustainable production processes. As a result, several studies have focused their attention on the reuse of grape by-products in the agro-food chain. Vine shoots, grape stalks, and wine lees, although produced to a lesser extent than grape pomace, have increasingly been receiving attention for their applications in the food sector, since they are a good source of functional and bioactive compounds. In this framework, our review highlights the promising results obtained by exploiting the antioxidant and/or antimicrobial activity of vine shoots, grape stalks, and wine lees or their extracts to replace the most common oenological additives and to assay the activity against food pathogens. Further, innovative functional foods and sustainable food packaging have been formulated by taking advantage of polyphenols and fiber, as well as plant bio-stimulants, in order to obtain grapes and wines with high quality characteristics. Overall, these by-products showed the potential to be recycled into the food chain as functional additives for different products and applications, supporting the sustainability of the winemaking sector.

Influence of Oxygen Availability on Cell Growth and Xylitol Production by Candida guilliermondii

2002 ◽  
pp. 449-458
Author(s):  
Luis F. Figueiredo Faria ◽  
Maria Antonieta P. Gimenes ◽  
Ronaldo Nobrega ◽  
Nei Pereira
Keyword(s):  
Cell Growth ◽  
Candida Guilliermondii ◽  
Oxygen Availability ◽  
Xylitol Production

scholarly journals Enzymatic Hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Scaffolds

Materials ◽  
2020 ◽  
Vol 13 (13) ◽  
pp. 2992 ◽  
Author(s):  
Adriana Kovalcik ◽  
Stanislav Obruca ◽  
Michal Kalina ◽  
Michal Machovsky ◽  
Vojtech Enev ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Saline Solution ◽  
Morphological Changes ◽  
Carbon Sources ◽  
Grape Pomace ◽  
Degree Of Crystallinity ◽  
Lignocellulosic Waste ◽  
Hydrolysis Of ◽  
The Degree Of Crystallinity ◽  
By Products

Polyhydroxyalkanoates (PHAs) are hydrolyzable bio-polyesters. The possibility of utilizing lignocellulosic waste by-products and grape pomace as carbon sources for PHA biosynthesis was investigated. PHAs were biosynthesized by employing Cupriavidus necator grown on fructose (PHBV-1) or grape sugar extract (PHBV-2). Fifty grams of lyophilized grape sugar extract contained 19.2 g of glucose, 19.1 g of fructose, 2.7 g of pectin, 0.52 g of polyphenols, 0.51 g of flavonoids and 7.97 g of non-identified rest compounds. The grape sugar extract supported the higher production of biomass and modified the composition of PHBV-2. The biosynthesized PHAs served as matrices for the preparation of the scaffolds. The PHBV-2 scaffolds had about 44.2% lower crystallinity compared to the PHBV-1 scaffolds. The degree of crystallinity markedly influenced the mechanical behavior and enzymatic hydrolysis of the PHA scaffolds in the synthetic gastric juice and phosphate buffer saline solution with the lipase for 81 days. The higher proportion of amorphous moieties in PHBV-2 accelerated enzymatic hydrolysis. After 81-days of lasting enzymatic hydrolysis, the morphological changes of the PHBV-1 scaffolds were negligible compared to the visible destruction of the PHBV-2 scaffolds. These results indicated that the presence of pectin and phenolic moieties in PHBV may markedly change the semi-crystalline character of PHBV, as well as its mechanical properties and the course of abiotic or enzymatic hydrolysis.

An improvement in fermentability of acid-hydrolysed hemicellulose from kenaf stem for xylitol production

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Siti Syazwani Mohd Shah ◽  
Abdullah Amru Indera Luthfi ◽  
Jamaliah Md Jahim ◽  
Shuhaida Harun ◽  
Kheng Oon Low
Keyword(s):  
Escherichia Coli ◽  
Adaptive Evolution ◽  
Production Performance ◽  
Xylitol Production ◽  
Dilute Acid ◽  
Fermentation Inhibitors ◽  
Dilute Acid Hydrolysis ◽  
Xylose Consumption ◽  
Inhibitory Compounds ◽  
Overall Performance

AbstractIn recent years, there has been a growing interest in the use of agricultural biomass for fermentation purposes; however, efficient strategies to counter lignocellulose inhibition are warranted to enhance xylitol production performance. Dilute-acid hydrolysis has been studied to selectively release a significant portion of xylose from hemicellulose, while leaving cellulose and lignin intact. The formation of inhibitory compounds, however, could jeopardise the overall performance during fermentation to produce xylitol. In this study, the fermentability of nitric acid-hydrolysed kenaf stem was substantially improved, through either adaptive evolution of the recombinant Escherichia coli BL21 (DE3) or removal of fermentation inhibitors by detoxification with activated carbon. Both methods were compared to evaluate the superiority in fermentative performance. In the fermentation with detoxified hemicellulosic hydrolysate, the non-adapted strain produced the highest xylitol concentration of up to 6.8 g/L, with 61.5% xylose consumption. The yields of xylitol production involving detoxification were successfully enhanced by 22.6% and by 35.7% compared to those involving adaptive evolution and raw hydrolysate, respectively. The results reported herein suggest that the utilization of detoxified kenaf stem hydrolysate could be advantageous.

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