scholarly journals Effects of Connective Tissue Growth Factor on the Cell Viability, Proliferation, Osteogenic Capacity and mRNA Expression of Stem Cell Spheroids

2021 ◽  
Vol 11 (14) ◽  
pp. 6572
Author(s):  
Abdullah Zaki Alnahash ◽  
Young-Min Song ◽  
Sae-Kyung Min ◽  
Hyun-Jin Lee ◽  
Min-Ji Kim ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Connective Tissue ◽  
Cell Viability ◽  
Mrna Expression ◽  
Connective Tissue Growth Factor ◽  
Tissue Growth ◽  
Alizarin Red S ◽  
Alizarin Red ◽  
Cell Spheroids

Background: Connective tissue growth factor (CTGF) is a cellular communication network factor family protein involved in many cellular functions. The purpose of this study was to determine the effects of CTGF on the proliferation, osteogenic capacity, and mRNA expression of spheroids composed of gingiva-derived mesenchymal stem cells (GMSCs). Methods: CTGF was applied at final concentrations of 0, 25, 50, 100, and 200 ng/mL. Qualitative cell viability was determined using Live/Dead kit assay. Metabolic viability was determined with a colorimetric assay kit. Osteogenic activity was analyzed with alkaline phosphatase activity and Alizarin Red S staining. Quantitative polymerase chain reaction (qPCR) was used to assess the expression levels of RUNX2, BSP, OCN, and COL1A1. Results: In general, there was no significant difference in cell viability between the groups on Days 1, 4, and 7. Addition of CTGF produced an increase in Alizarin Red S staining. qPCR results demonstrated that the mRNA expression levels of RUNX2, BSP, OCN, and COL1A1 were significantly increased with the addition of CTGF. Conclusions: Based on these findings, we conclude that CTGF can be applied for increased osteogenic differentiation of stem cell spheroids.

scholarly journals Dynamic Vibration Cooperates with Connective Tissue Growth Factor to Modulate Stem Cell Behaviors

2014 ◽  
Vol 20 (13-14) ◽  
pp. 1922-1934 ◽  
Author(s):  
Zhixiang Tong ◽  
Aidan B. Zerdoum ◽  
Randall L. Duncan ◽  
Xinqiao Jia
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Connective Tissue ◽  
Connective Tissue Growth Factor ◽  
Tissue Growth ◽  
Cell Behaviors ◽  
Dynamic Vibration

scholarly journals Kinetics of Connective Tissue Growth Factor Expression during Experimental Proliferative Glomerulonephritis

2001 ◽  
Vol 12 (3) ◽  
pp. 472-484
Author(s):  
YASUHIKO ITO ◽  
ROEL GOLDSCHMEDING ◽  
RICHARD J. BENDE ◽  
NIKE CLAESSEN ◽  
M. ANWAR CHAND ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Connective Tissue ◽  
Mrna Expression ◽  
Connective Tissue Growth Factor ◽  
Mesangial Cells ◽  
Tissue Growth ◽  
Proliferative Glomerulonephritis ◽  
Kinetics Of ◽  
Tgf Β1

Abstract. Connective tissue growth factor (CTGF) is a member of the CCN family of immediate early genes, which are involved in cell proliferation, migration, and matrix production. Recently, CTGF was observed to be strongly upregulated in human proliferative and fibrogenic renal disease. By in situ hybridization and reverse transcriptase-PCR, the expression of CTGF was investigated in experimental proliferative glomerulonephritis induced by injection of anti—Thy-1.1 antibody in the rat. CTGF expression in cultured rat mesangial cells and glomerular visceral epithelial cells (GVEC) was studied in response to transforming growth factor β (TGF-β), an essential pathogenetic factor in this model. In normal rat kidneys, only some GVEC expressed CTGF mRNA. In anti—Thy-1.1 nephritis, CTGF mRNA expression was strongly increased in extracapillary and mesangial proliferative lesions and in areas of periglomerular fibrosis. Early glomerular CTGF overexpression in GVEC coincided with a striking upregulation of TGF-β2 and to a lesser extent of TGF-β3. Glomerular CTGF mRNA expression was maximal at day 7, in association with increased TGF-β1 mRNA and protein expression. CTGF mRNA overexpression by parietal epithelial cells preceded the periglomerular appearance of α-smooth muscle actin—positive fibroblasts. In cultured mesangial cells, TGF-β1, -β2, and -β3 transiently increased the CTGF/glyceraldehyde phosphate dehydrogenase mRNA ratio up to threefold versus control at 4 h. In GVEC, upregulation of CTGF mRNA by these TGF-β isoforms was more sustained, being 8- to 16-fold versus control at 24 h. The kinetics of CTGF expression strongly suggest a role in glomerular repair, possibly downstream of TGF-β, in this model of transient renal injury.

scholarly journals Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell-Derived Choroidal Endothelium

2017 ◽  
Vol 6 (6) ◽  
pp. 1533-1546 ◽  
Author(s):  
Allison E. Songstad ◽  
Kristan S. Worthington ◽  
Kathleen R. Chirco ◽  
Joseph C. Giacalone ◽  
S. Scott Whitmore ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Connective Tissue ◽  
Connective Tissue Growth Factor ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Tissue Growth ◽  
Efficient Generation ◽  
Induced Pluripotent

Human Connective Tissue Growth Factor mRNA Expression of Epiretinal and Subretinal Fibrovascular Membranes: A Report of Three Cases

2002 ◽  
Vol 216 (4) ◽  
pp. 284-291 ◽  
Author(s):  
Peter Meyer ◽  
Kerstin Wunderlich ◽  
Hermann L. Kain ◽  
Christian Prünte ◽  
Josef Flammer
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Mrna Expression ◽  
Connective Tissue Growth Factor ◽  
Tissue Growth

scholarly journals Hypoxia Induces Connective Tissue Growth Factor mRNA Expression

2009 ◽  
Vol 24 (Suppl 1) ◽  
pp. S176 ◽  
Author(s):  
Young Ki Lee ◽  
Eun-Ji Kim ◽  
Jung Eun Lee ◽  
Jung Woo Noh ◽  
Yoon-Goo Kim
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Mrna Expression ◽  
Connective Tissue Growth Factor ◽  
Tissue Growth

scholarly journals Expression and regulation of osteopontin and connective tissue growth factor transcripts in rat anterior pituitary

2001 ◽  
Vol 169 (1) ◽  
pp. 87-96 ◽  
Author(s):  
J Ehrchen ◽  
H Heuer ◽  
R Sigmund ◽  
MK Schafer ◽  
K Bauer
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Mrna Expression ◽  
Connective Tissue Growth Factor ◽  
Anterior Pituitary ◽  
Tissue Growth ◽  
Cell Interactions ◽  
Female Rats ◽  
Transcript Levels ◽  
Cell Cell

Cell-cell interactions are important regulatory elements in anterior pituitary (AP) physiology. As model systems to study pituitary cell-cell interactions, AP cells kept either as monolayers or as organotypic reaggregate cultures were analyzed by differential display PCR. We identified six cDNA fragments (osteopontin (Opn), connective tissue growth factor (CTGF), alpha(v)-integrin, cathepsin H, lysozyme and O-acetyl GD(3) ganglioside synthase) that showed elevated expression in monolayers compared with reaggregate cultures and the AP. The adenohypophyseal mRNA expression of Opn and CTGF, two secreted signaling substances, was studied in more detail. In situ hybridization histochemistry revealed that Opn mRNA expression is restricted to a subpopulation of gonadotropes whereas CTGF hybridization signals could not be ascribed to any known cell type. Opn transcript levels were downregulated in the APs of lactating rats and decreased when rats received s.c. injections of 17beta-estradiol for 5 days. The mRNA expression was higher in male than in female rats and increased after gonadectomy. CTGF transcript levels were higher in male compared with female rats and were increased in pregnant rats and in rats treated for 5 days with triiodothyronine or dexamethasone. These results indicate that Opn and CTGF may be of physiological importance as local communication factors in the AP.

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