scholarly journals Advanced Platelet-Rich Fibrin as a Therapeutic Option in the Treatment of Dry Socket: Literature Review and Case Series

2021 ◽  
Vol 11 (20) ◽  
pp. 9474
Author(s):  
Gaetano Marenzi ◽  
Roberta Gasparro ◽  
Mauro Mariniello ◽  
Gilberto Sammartino ◽  
Claudia Capone ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Surgery ◽  
Therapeutic Option ◽  
Healing Process ◽  
Case Series ◽  
Wound Healing Process ◽  
Platelet Rich Fibrin ◽  
Dry Socket ◽  
Operative Recovery ◽  
Dependent Samples

Alveolar osteitis (AO) is one of the complications that occur after tooth extraction. The aim of this study has been to evaluate the efficacy of Advanced Platelet-rich Fibrin (A-PRF) in the management of pain and the acceleration of wound healing in the treatment of AO. Consecutive patients who were diagnosed with AO, recruited from patients referred to the Oral Surgery Department of the University of Naples Federico II, were enrolled. After local anesthesia, the dry socket was curetted and irrigated with saline. The Platelet-rich Fibrin (PRF) clot was placed in the socket and then covered with an A-PRF membrane. Clinical parameters, such as the degree of pain and rate of granulation tissue (GT) formation, were measured before treatment and after 1, 3, 7, 14, and 21 days. The Friedman test for dependent samples was used to detect the treatment and time effect. Four patients with established AO were included. On all the examination days, the post-operative recovery was uneventful. The pain scores progressively reduced, from an average of 8.5 before treatment to 0.25 on the third day, and the GT formation improved over time. The use of A-PRF in the treatment of AO significantly reduced the pain level and enhanced the wound-healing process.

Wireless Electrical Stimulation

2013 ◽  
Vol 12 (1) ◽  
pp. 18-21 ◽  
Author(s):  
Ourania Castana ◽  
Aekaterini Dimitrouli ◽  
Theodoros Argyrakos ◽  
Emilia Theodorakopoulou ◽  
Nektarios Stampolidis ◽  
...  
Keyword(s):  
Wound Healing ◽  
Electrical Stimulation ◽  
Beneficial Effect ◽  
Lower Limb ◽  
Therapeutic Option ◽  
Healing Process ◽  
Wound Healing Process ◽  
Effective Therapeutic Option ◽  
Chronic Ulcers ◽  
Stimulation Technique

High-voltage electrical stimulation has been long proposed as a method of accelerating the wound healing process. Its beneficial effect has been successfully evaluated in the treatment of a number of chronic ulcers and burns. We present here the implementation of a new wireless electrical stimulation technique for the treatment of a complicated chronic ulcer of the lower limb. The device is transferring charges to the wound, without any contact with it, creating a microcurrent that is able to generate the current of injury. The results suggest that this easy-to-use method is an effective therapeutic option for chronic ulcers.

scholarly journals Modified Preparation Method of Ideal Platelet-Rich Fibrin Matrix (PRFM) from Whole Blood.

2021 ◽  
Author(s):  
Mirta Hediyati Reksodiputro ◽  
Alida Roswita Harahap ◽  
Lyana Setiawan ◽  
Mikhael Yosia
Keyword(s):  
Wound Healing ◽  
Whole Blood ◽  
Preparation Method ◽  
Healing Process ◽  
Wound Healing Process ◽  
Platelet Rich Fibrin ◽  
Platelet Counts ◽  
Fibrin Matrix ◽  
Modified Method ◽  
Commercial Kits

One bioproduct that is widely used in the wound healing process is Platelet Rich Plasma (PRP). PRP is a liquid solution with high autologous platelet concentration, making it a good source of growth factors to accelerate wound healing. Recent development in PRP had created a new product called Platelet Rich Fibrin Matrix (PRFM), which has a denser and more flexible structure. PRFM is the newest generation of platelet concentrate with a fibrin matrix that holds platelet in it. The key concept in creating PRFM from PRP is the addition of CaCl2 followed by centrifugation, which converts fibrinogen to fibrin, and the fibrin cross-links to form a matrix that contains viable platelets. There are many commercially available kits to create PRFM, but they are often expensive and uneconomical. This research will test a modified method of making ideal PRFM from PRP without any commercial kits. The modified method will include determining the minimum level of CaCl2 used, the type of centrifuge, and the speed and duration of centrifugation. By performing a modified preparation method on five samples of whole blood, it was found that the ideal PRFM could be made by mixing PRP with 25 mM CaCl2 1M and centrifuging it at a speed of 2264 G for 25 minutes at room temperature. The PRP and PRFM platelet counts of this method tend to be lower than the platelet counts found in other studies. Although visually comparable, further study is needed to compare the performance of PRFMs made with this method and PRFMs made with commercial kits.

scholarly journals A study on new experimental induction of wound using metal surface contact in a rat model

2020 ◽  
Vol 11 (1) ◽  
pp. 813-817
Author(s):  
Balaji K ◽  
Perumal Saraswathi ◽  
Prabhu K ◽  
Shila Samuel ◽  
Siva T
Keyword(s):  
Wound Healing ◽  
Metal Surface ◽  
Therapeutic Option ◽  
Healing Process ◽  
Research Progress ◽  
Albino Rats ◽  
Wound Healing Process ◽  
Contact Method ◽  
Burn Wound ◽  
Surface Contact

Burns injury is a global issue; when burns left unmonitored, it may lead to sepsis & hypovolemia. It majorly contributing to the country's morbidity and mortality. Accidental burns are quite common throughout the world. The degree of burns differs variably, and to identify a possible therapeutic option, it is of great importance for the researchers and scientists. Achieving a uniform burn wound model is thus required to study the burns injury at different levels involving epidermis, dermis, and hypodermis. The goal of this study is to create a simple third-degree contact burn in Wistar albino rats. Third-degree contact burn wound was monitored on 7th, 14th, and 21st day for natural wound healing under gross and histopathological observation. Gross observation reported damaged skin to appear dry, leathery, and charred. Wound closure was observed to be delayed and incomplete on day 21. Histopathology revealed a wound healing process by hematoxylin and eosin method. This metal surface contact method revealed a simplified and cost-effective method for inducing a third degree of a wound in Wistar rats. The study is thus a milestone that could enable research progress in the area of wound healing.   

Granulox—The Use of Topical Hemoglobin to Aid Wound Healing: A Literature Review and Case Series From Singapore

2020 ◽  
pp. 153473462091031
Author(s):  
Clement Loh ◽  
Qian Ying Tan ◽  
Diane L. K. Eng ◽  
Stewart R. Walsh ◽  
Tze Tec Chong ◽  
...  
Keyword(s):  
Wound Healing ◽  
Clinical Evidence ◽  
Chronic Wounds ◽  
Healing Process ◽  
Symptom Relief ◽  
Case Series ◽  
Supplemental Oxygen ◽  
Wound Healing Process ◽  
Portable Devices ◽  
Facilitated Diffusion

Chronic wounds are an increasing burden on health care globally, and tissue hypoxia is a common issue in such wounds. Granulox (SastoMed GmbH, Georgsmarienhütte, Germany) is designed to provide wound bases with supplemental oxygen through facilitated diffusion and aid the physiological wound-healing process. It is a topical hemoglobin wound spray that is applied after wound cleaning and debridement to ensure continuous oxygen supplementation for up to 72 hours. Compared with other forms of topical oxygen therapy that require portable devices, Granulox is a spray-on application and the patient experience is similar to the use of a conventional dressing. Current clinical evidence suggests that Granulox aids with both wound healing and with symptom relief in chronic wounds, but current literature is limited by small study populations and further larger studies are required.

scholarly journals Pre-Clinical Study: Immunohistochemical evaluation of matrix metalloproteinase-13 on rabbit (Oryctolagus cuniculus) socket healing after application of platelet-rich fibrin with and without hydroxyapatite

F1000Research ◽  
2022 ◽  
Vol 11 ◽  
pp. 29
Author(s):  
META MAULIDA DAMAYANTI ◽  
MEIKE RACHMAWATI
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinase ◽  
Oryctolagus Cuniculus ◽  
Healing Process ◽  
Observation Time ◽  
Control Group ◽  
Wound Healing Process ◽  
Platelet Rich Fibrin ◽  
Matrix Metalloproteinase 13 ◽  
Socket Healing

Background: Tissue engineering technology has been used globally and proven to accelerate wound healing. This study aimed to analyse the effect of adding hydroxyapatite (HA) as a scaffold to platelet-rich fibrin (PRF) as a growth factor in accelerating the wound healing process as seen from the expression of matrix metalloproteinase-13 (MMP-13). Methods: This research is an animal experiment conducted on 18 rabbits (Oryctolagus cuniculus). Rabbits were randomly divided into the following three groups of treatment: (G1) the application of PRF group, (G2) the application of PRF+HA group and (C) the control group without any application. Furthermore, each treatment group was split randomly into three groups of observation time. Periodontal tissue biopsy was performed to analyse the histopathological features that were examined on the basis of the level of MMP-13 immunoexpression. Results: MMP-13 immunoexpression in the PRF+HA group showed better histoscore results, indicating a substantial reduction in MMP-13 values compared with other groups. The healing process was shown to increase with increasing observation time (p<0.05), and the PRF+HA group outperformed the PRF and control groups. On day 3, MMP-13 exhibited a dark brown colour of Immunohistochemistry (IHC), which indicated an increase in the expression value of MMP-13 in the early stages of healing, namely, inflammation. On day 14, light brown IHC was seen, especially in group 2, as a reference that the remodeling process had begun. Conclusions: This study indicates that the application of HA can accelerate the socket healing process by decreasing the level of immunoexpression of MMP-13. HA is an alloplastic material that has inherent bioactive properties that support osteoconduction, which functions as a scaffold in the form of a fibrin matrix that can bind MMPs so that it can accelerate the wound healing process.

scholarly journals A Modified Preparation Method of Ideal Platelet-Rich Fibrin Matrix From Whole Blood

2021 ◽  
Vol 8 ◽  
Author(s):  
Mirta Hediyati Reksodiputro ◽  
Alida Roswita Harahap ◽  
Lyana Setiawan ◽  
Mikhael Yosia
Keyword(s):  
Wound Healing ◽  
Whole Blood ◽  
Preparation Method ◽  
Healing Process ◽  
Wound Healing Process ◽  
Platelet Rich Fibrin ◽  
Platelet Counts ◽  
Fibrin Matrix ◽  
Modified Method ◽  
Commercial Kits

One bioproduct that is widely used in the wound healing process is platelet-rich plasma (PRP). PRP is a liquid solution with high autologous platelet concentration, making it a good source of growth factors to accelerate wound healing. Recent development in PRP had created a new product called platelet-rich fibrin matrix (PRFM), which has a denser and more flexible structure. PRFM is the newest generation of platelet concentrate with a fibrin matrix that holds platelet in it. The key concept in creating PRFM from PRP is the addition of CaCl2 followed by centrifugation, which converts fibrinogen to fibrin, and the fibrin cross-links to form a matrix that contains viable platelets. There are many commercially available kits to create PRFM, but they are often expensive and uneconomical. This research will test a modified method of making ideal PRFM from PRP without any commercial kits. The modified method will include determining the minimum level of CaCl2 used, the type of centrifuge, and the speed and duration of centrifugation. By performing a modified preparation method on five samples of whole blood, it was found that the ideal PRFM could be made by mixing PRP with 25 mM CaCl2 and centrifuging it at a speed of 2,264 × g for 25 min at room temperature. The PRP and PRFM platelet counts of this method tend to be lower than the platelet counts found in other studies. Although visually comparable, further study is needed to compare the performance of PRFMs made with this method and PRFMs made with commercial kits.

scholarly journals Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1122
Author(s):  
Nutda Sutthammikorn ◽  
Volaluck Supajatura ◽  
Hainan Yue ◽  
Miho Takahashi ◽  
Sunee Chansakaow ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Therapeutic Option ◽  
Healing Process ◽  
Unmet Need ◽  
New Drugs ◽  
Wound Healing Process ◽  
Diabetic Mice ◽  
Diabetic Wounds

Nonhealing wounds are major socioeconomic challenges to healthcare systems worldwide. Therefore, there is a substantially unmet need to develop new drugs for wound healing. Gynura procumbens, a herb found in Southeast Asia, may be an effective therapeutic for nonhealing diabetic wounds. The aim of this study was to evaluate the efficacy of G. procumbens on wound healing in the diabetic milieu. G. procumbens extract was obtained using 95% ethanol and its components were determined by thin layer chromatography. Diabetes was induced in mice using streptozotocin. We found that G. procumbens extract contained stigmasterol, kaempferol and quercetin compounds. Topical application of G. procumbens on the wounded skin of diabetic mice accelerated wound healing and induced the expression of angiogenin, epidermal growth factor, fibroblast growth factor, transforming growth factor and vascular endothelial growth factor. Furthermore, G. procumbens promoted in vitro wound healing and enhanced the migration and/or proliferation of human endothelial cells, fibroblasts, keratinocytes and mast cells cultured in diabetic conditions. Finally, G. procumbens promoted vascular formation in the diabetic mice. To the best of our knowledge, this is the first study that evaluates in vivo wound healing activities of G. procumbens and activation of cells involved in wound healing process in diabetic conditions. The findings that G. procumbens accelerates wound healing and activates cells involved in the wound healing process suggest that G. procumbens might be an effective alternative therapeutic option for nonhealing diabetic wounds.

scholarly journals Canine-Origin Platelet-Rich Fibrin as an Effective Biomaterial for Wound Healing in Domestic Cats: A Preliminary Study

2021 ◽  
Vol 8 (10) ◽  
pp. 213
Author(s):  
Carla S. Soares ◽  
Isabel R. Dias ◽  
Maria A. Pires ◽  
Pedro P. Carvalho
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Cost Effective ◽  
Wound Healing Process ◽  
Platelet Rich Fibrin ◽  
Cost Effective Treatment ◽  
Antiseptic Treatment ◽  
High Concentrations ◽  
Biological Cost ◽  
Treatment Of Wounds

Platelet-rich fibrin (PRF) is a recent platelet-based biomaterial, poised as an innovative regenerative strategy for the treatment of wounds from different etiologies. PRF is defined as a biodegradable scaffold containing elevated amounts of platelets and leukocytes having the capability to release high concentrations of bioactive structural proteins and acting as a temporal release healing hemoderivative. This study aimed to evaluate the performance of canine-origin PRF, obtained from blood of screened donors, as a regenerative biomaterial suitable for the treatment of critical wounds in felines. Four short-hair felines with naturally occurring wounds were enrolled in this study. Three of the wounds were considered infected. Each PRF treatment was the result of the grafting of newly produced PRFs at the recipient area. The PRF treatment was initially performed two to three times per week, followed by single weekly treatments. The study was finalized when complete wound closure was achieved. No topical antimicrobial/antiseptic treatment was applied. The present research demonstrated that xenogenic PRFs significantly induced healthy vascularized granulation tissue in lesions with soft tissue deficit, also prompting the epithelization at the injured site. No rejection, necrosis, or infection signs were recorded. Additionally, PRF-therapy was revealed to be a biological cost-effective treatment, accelerating the wound healing process.

Platelet-Rich Fibrin Enhances Surgical Wound Healing in Total Laryngectomy

2021 ◽  
Author(s):  
Mirta H. Reksodiputro ◽  
Syahrial M. Hutauruk ◽  
Dini W. Widodo ◽  
Fauziah Fardizza ◽  
Dita Mutia
Keyword(s):  
Wound Healing ◽  
Health Care Providers ◽  
Total Laryngectomy ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Pharyngocutaneous Fistula ◽  
Care Providers ◽  
Platelet Rich Fibrin ◽  
Postoperative Wound

AbstractAutologous growth factor (AGF) is a cytokine that has gained medical research interest because it helps improve and accelerate the wound healing process. Platelet-rich fibrin (PRF) is the latest generation of platelet concentrate that can be obtained through a simple procedure known as AGF referencing. One of the most common complications of total laryngectomy (TL) is pharyngocutaneous fistula. To prevent this complication, health care providers must closely monitor the postoperative wound healing process.This study aimed to investigate the effectiveness of PRF application in enhancing wound healing after TL. A randomized controlled trial was conducted in the Department of Otorhinolaryngology - Head and Neck Surgery, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital Universitas Indonesia, Jakarta, Indonesia, from June 2019 to December 2019. We included 20 patients who underwent TL for laryngeal squamous cell carcinoma. They were divided into two groups (10 patients who received applied autologous PRF around the esophageal stoma during TL and another 10 patients as the control). These patients were observed for 2 weeks postoperatively. In the bivariate analysis performed using the chi-square test, the pain threshold and edema of postoperative wounds in the PRF-treated group demonstrated significant differences compared with those in the control group. PRF application in TL enhanced the postoperative wound healing process, especially with regard to edema and pain.

scholarly journals Platelet-Rich Fibrin as a Biofuel for Tissue Regeneration

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Sujeet Vinayak Khiste ◽  
Ritam Naik Tari
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Clinical Application ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Vital Role ◽  
Wound Healing Process ◽  
Platelet Rich Fibrin ◽  
Fibrin Network ◽  
New Material

Wound healing is a staged process which involves the activity of leukocytes and platelets. For this process to work efficiently, the platelets play a vital role. The growth factors present in platelets are important to guide the regenerating cells to the area of healing. Platelet-rich-fibrin (PRF) is one such material that holds on to these growth factors enmeshed in the fibrin network resulting in their sustained release over a period of time that can accelerate the wound healing process. With this knowledge, research has been carried out for a past few years for the clinical application of PRF. Various platelet concentrates have been studied including the platelet-rich-plasma (PRP). However, the short duration of cytokine release and its poor mechanical properties have resulted in the search of a new material with adequate properties for clinical application and ease of preparation. PRF has found a place in the regenerative field owing to its advantages over PRP. This review focuses on the properties and various applications of PRF in the clinical practice.

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