scholarly journals High-Resolution Mass Spectrometry Identification of Secondary Metabolites in Four Red Grape Varieties Potentially Useful as Traceability Markers of Wines

Beverages ◽  
2018 ◽  
Vol 4 (4) ◽  
pp. 74 ◽  
Author(s):  
Christine Mayr ◽  
Mirko De Rosso ◽  
Antonio Dalla Vedova ◽  
Riccardo Flamini
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Secondary Metabolites ◽  
High Resolution Mass Spectrometry ◽  
High Resolution Mass ◽  
Mass Spectrometry Identification ◽  
Red Grape ◽  
Grape Varieties ◽  
Acyl Derivatives ◽  
Resolution Mass

Liquid chromatography coupled to high-resolution mass spectrometry (LC-Q/TOF) is a powerful tool to perform chemotaxonomic studies through identification of grape secondary metabolites. In the present work, the metabolomes of four autochthonous Italian red grape varieties including the chemical classes of anthocyanins, flavonols/flavanols/flavanones, and terpenol glycosides, were studied. By using this information, the metabolites that can potentially be used as chemical markers for the traceability of the corresponding wines were proposed. In Raboso wines, relatively high abundance of both anthocyanic and non-anthocyanic acyl derivatives, is expected. Potentially, Primitivo wines are characterized by high tri-substituted flavonoids, while Corvina wines are characterized by higher di-substituted compounds and lower acyl derivatives. Negro Amaro wine’s volatile fraction is characterized by free monoterpenes, such as α-terpineol, linalool, geraniol, and Ho-diendiol I. A similar approach can be applied for the traceability of other high-quality wines.

Compositional analysis of manuka honeys by high-resolution mass spectrometry: Identification of a manuka-enriched archetypal molecule

2012 ◽  
Vol 132 (2) ◽  
pp. 948-953 ◽  
Author(s):  
Liam Fearnley ◽  
David R. Greenwood ◽  
Michael Schmitz ◽  
Jonathan M. Stephens ◽  
Ralf C. Schlothauer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Compositional Analysis ◽  
High Resolution Mass ◽  
Mass Spectrometry Identification ◽  
Resolution Mass

scholarly journals Chemotaxonomic Profiling of Canadian Alternaria Populations Using High-Resolution Mass Spectrometry

Metabolites ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 238
Author(s):  
Megan J. Kelman ◽  
Justin B. Renaud ◽  
Keith A. Seifert ◽  
Jonathan Mack ◽  
Ken K.-C. Yeung ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Secondary Metabolites ◽  
Plant Pathogens ◽  
High Resolution Mass Spectrometry ◽  
Principal Component ◽  
Species Level ◽  
Production Chain ◽  
High Resolution Mass ◽  
Resolution Mass

Alternaria spp. occur as plant pathogens worldwide under field and storage conditions. They lead to food spoilage and also produce several classes of secondary metabolites that contaminate the food production chain. From a food safety perspective, the major challenge of assessing the risk of Alternaria contamination is the lack of a clear consensus on their species-level taxonomy. Furthermore, there are currently no reliable DNA sequencing methods to allow for differentiation of the toxigenic potential of these fungi. Our objective was to determine which species of Alternaria exist in Canada, and to describe the compounds they make. To address these issues, we performed metabolomic profiling using liquid chromatography high-resolution mass spectrometry (LC-HRMS) on 128 Canadian strains of Alternaria to determine their chemotaxonomy. The Alternaria strains were analyzed using principal component analysis (PCA) and unbiased k-means clustering to identify metabolites with significant differences (p < 0.001) between groups. Four populations or ‘chemotypes’ were identified within the strains studied, and several known secondary metabolites of Alternaria were identified as distinguishing metabolites, including tenuazonic acid, phomapyrones, and altenuene. Though species-level identifications could not be concluded for all groups through metabolomics alone, A. infectoria was able to be identified as a distinct population.

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