scholarly journals Degradation of Veterinary Antibiotics in Swine Manure via Anaerobic Digestion

2020 ◽  
Vol 7 (4) ◽  
pp. 123 ◽  
Author(s):  
Ali Hosseini Taleghani ◽  
Teng-Teeh Lim ◽  
Chung-Ho Lin ◽  
Aaron C. Ericsson ◽  
Phuc H. Vo
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Water Solution ◽  
Swine Manure ◽  
Amplicon Sequencing ◽  
Veterinary Antibiotics ◽  
Operating Variables ◽  
Working Volume ◽  
Before And After ◽  
Veterinary Antibiotic

Antibiotic-resistant microorganisms are drawing a lot of attention due to their severe and irreversible consequences on human health. The animal industry is considered responsible in part because of the enormous volume of antibiotics used annually. In the current research, veterinary antibiotic (VA) degradation, finding the threshold of removal and recognizing the joint effects of chlortetracycline (CTC) and Tylosin combination on the digestion process were studied. Laboratory scale anaerobic digesters were utilized to investigate potential mitigation of VA in swine manure. The digesters had a working volume of 1.38 L (in 1.89-L glass jar), with a hydraulic retention time (HRT) of 21 days and a loading rate of 1.0 g-VS L−1 d−1. Digesters were kept at 39 ± 2 °C in incubators and loaded every two days, produced biogas every 4 days and digester pH were measured weekly. The anaerobic digestion (AD) process was allowed 1.5 to 2 HRT to stabilize before adding the VAs. Tests were conducted to compare the effects of VAs onto manure nutrients, volatile solid removal, VA degradation, and biogas production. Concentrations of VA added to the manure samples were 263 to 298 mg/L of CTC, and 88 to 263 mg/L of Tylosin, respectively. Analysis of VA concentrations before and after the AD process was conducted to determine the VA degradation. Additional tests were also conducted to confirm the degradation of both VAs dissolved in water under room temperature and digester temperature. Some fluctuations of biogas production and operating variables were observed because of the VA addition. All CTC was found degraded even only after 6 days of storage in water solution; thus, there was no baseline to estimate the effects of AD. As for Tylosin, 100% degradation was observed due to the AD (removal was 100%, compared with 24–40% degradation observed in the 12-day water solution storage). Besides, complete Tylosin degradation was also observed in the digestate samples treated with a mixture of the two VAs. Lastly, amplicon sequencing was performed on each group by using the 50 most variable operational taxonomic units (OTUs)s and perfect discriminations were detected between groups. The effect of administration period and dosage of VAs on Phyla Firmicutes Proteobacteria, Synergistetes and Phylum Bacteroides was investigated. These biomarkers’ abundance can be employed to predict the sample’s treatment group.

scholarly journals Degradation of the veterinary antibiotics in swine manure via anaerobic digestion

2021 ◽  
Author(s):  
◽  
Ali Hosseini Taleghani
Keyword(s):  
Solid Phase ◽  
Biogas Production ◽  
Swine Manure ◽  
Carbon Dioxide Concentration ◽  
Amplicon Sequencing ◽  
Sequencing Analysis ◽  
Veterinary Antibiotics ◽  
Working Volume ◽  
Before And After ◽  
Major Alteration

Antimicrobial resistance is becoming an imminent danger for human health, in part because of excessive consumption of antibiotics in the animal industry. Laboratory-scale anaerobic digesters (ADs) were utilized to investigate the potential removal of veterinary antibiotics (VA) in swine manure. Nine laboratory ADs were kept at 39 [plus-minus] 2 [degrees] C in incubators and were loaded every two days. The digesters had a working volume of 1.38 L (in a 1.89-L glass jar), with a hydraulic retention time (HRT) of 21 days and a loading rate of 1.0 g-VS/L-day. Carbon dioxide concentration of the biogas and pH values of the digestate verifying digester performance were measured every four days and every week, respectively. The AD process allowed 2 HRT to stabilize before the addition of the VAs, of Chlortetracycline (CTC), Tylosin, and Sulfamethazine (SMZ). Tests were conducted to compare the effects of VAs on manure nutrients, volatile solid destruction, and biogas production. Concentrations of VA added to the manure samples were 263 to 298 mg/L of CTC, 88 to 263 mg/L of Tylosin, and 1042 to 1339 mg/L of SMZ, respectively. Analysis of VA concentrations before and after the AD process was conducted to determine the VA degradation. The process of measuring the relatively low VA concentrations involves using solid-phase extraction and LC-MS-MS methodology. Further tests were performed in water under room temperature and digester temperature to approve the degradation of VAs. Amplicon sequencing analysis performed on each group using the 50 most frequent amplicon sequence variants (ASVs) showed significant discriminations between groups. A hierarchical method (UPGMA) was applied to obtain the major alteration in bacterial dynamics between treatments. The biomarkers' abundance can be employed to distinguish different antibiotics contamination.

scholarly journals Biochemical Methane Potential of Cork Boiling Wastewater at Different Inoculum to Substrate Ratios

2021 ◽  
Vol 11 (7) ◽  
pp. 3064
Author(s):  
Roberta Mota-Panizio ◽  
Manuel Jesús Hermoso-Orzáez ◽  
Luis Carmo-Calado ◽  
Gonçalo Lourinho ◽  
Paulo Sérgio Duque de Brito
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Treatment Plant ◽  
Organic Substrates ◽  
Biochemical Methane Potential ◽  
Volatile Solids ◽  
Working Volume ◽  
Methane Potential ◽  
Bmp Test ◽  
Cork Industry

The present study evaluates the digestion of cork boiling wastewater (CBW) through a biochemical methane potential (BMP) test. BMP assays were carried out with a working volume of 600 mL at a constant mesophilic temperature (35 °C). The experiment bottles contained CBW and inoculum (digested sludge from a wastewater treatment plant (WWTP)), with a ratio of inoculum/substrate (Ino/CBW) of 1:1 and 2:1 on the basis of volatile solids (VSs); the codigestion with food waste (FW) had a ratio of 2/0.7:0.3 (Ino/CBW:FW) and the codigestion with cow manure (CM) had a ratio of 2/0.5:0.5 (Ino/CBW:CM). Biogas and methane production was proportional to the inoculum substrate ratio (ISR) used. BMP tests have proved to be valuable for inferring the adequacy of anaerobic digestion to treat wastewater from the cork industry. The results indicate that the biomethane potential of CBWs for Ino/CBW ratios 1:1 and 2:1 is very low compared to other organic substrates. For the codigestion tests, the test with the Ino/CBW:CM ratio of 2/0.7:0.3 showed better biomethane yields, being in the expected values. This demonstrated that it is possible to perform the anaerobic digestion (AD) of CBW using a cosubstrate to increase biogas production and biomethane and to improve the quality of the final digestate.

Degradation of veterinary antibiotics during anaerobic digestion of dairy manure

2015 ◽  
Vol 10 (3) ◽  
pp. 532-537 ◽  
Author(s):  
Yumika Kitazono ◽  
Ikko Ihara ◽  
Kiyohiko Toyoda ◽  
Kazutaka Umetsu
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Dairy Manure ◽  
Antibiotic Residues ◽  
Veterinary Antibiotics ◽  
Livestock Manure ◽  
Anaerobic Digestion Process ◽  
Digestion Process ◽  
Antibiotic Degradation

This study evaluated antibiotic degradation and biogas production during anaerobic digestion of dairy manure contained two common veterinary antibiotics at 37 °C. After 18 days of digestion, the concentration of chlortetracycline (CTC) decreased more than 80% regardless of the initial CTC concentration. The concentration of cefazolin (CEZ) decreased from 10 to 0.08 mg/L in 6 days. Less than 50 mg/L CTC and 10 mg/L CEZ had negligible impact on biogas production during anaerobic digestion process. The result showed that the anaerobic digestion has a potential to degrade antibiotic residues in livestock manure.

Improved biogas production of dry anaerobic digestion of swine manure

2019 ◽  
Vol 294 ◽  
pp. 122188 ◽  
Author(s):  
Youqian Xiao ◽  
Hongnan Yang ◽  
Han Yang ◽  
Hong Wang ◽  
Dan Zheng ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Swine Manure ◽  
Dry Anaerobic Digestion

Anaerobic digestion of chicken feather with swine manure or slaughterhouse sludge for biogas production

2012 ◽  
Vol 32 (3) ◽  
pp. 404-409 ◽  
Author(s):  
Yun Xia ◽  
Daniel I. Massé ◽  
Tim A. McAllister ◽  
Carole Beaulieu ◽  
Emilio Ungerfeld
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Swine Manure ◽  
Chicken Feather

scholarly journals The effect of A. Fumigatus SK1 and trichoderma sp. on the biogas production from cow manure

2018 ◽  
Vol 14 (3) ◽  
pp. 353-359 ◽  
Author(s):  
Zulfah Zulkifli ◽  
Nazaitulshila Rasit ◽  
Noor Azrimi Umor ◽  
Shahrul Ismail
Keyword(s):  
Anaerobic Digestion ◽  
Lignocellulosic Biomass ◽  
Biogas Production ◽  
Reducing Sugar ◽  
Treatment Result ◽  
Cow Manure ◽  
Lignin Removal ◽  
Trichoderma Sp ◽  
Working Volume ◽  
Pre Treatment

Lignocellulosic material consists of lignin, cellulose and hemicellulose. Converting lignocellulosic biomass such as cow manure (CM) into value-added products provides a potential alternative. Hydrolysis of cellulose and hemicellulose is a limiting step during Anaerobic Digestion (AD) of lignocellulosic biomass. Lignin in lignocellulosic biomass is the barrier for hydrolysis, thus limits the biogas production. In this study, the effect of A.Fumigatus SK1 and Trichoderma sp. on enzymatic pre-treatment of CM was investigated with respect to the biogas production. Three set of anaerobic digestion assays were carried out, with a working volume of 500 mL at 35 ± 2°C and 120 rpm. The first set of fermentation contained untreated CM. The second set of fermentation involved addition of A.Fumigatus SK1, and the last set contained Trichoderma sp. Several analysis were conducted to determine the biomethane potential (BMP), anaerobic biodegradability, reducing sugars concentration and lignin removal of CM before and after pre-treatment. Result showed that, among both evaluated pre-treatment methods, CM treated with Trichoderma sp. gave the highest methane potential with 0.023 LCH4-STP g VS-1 compared to CM treated with A.Fumigatus SK1(0.011 LCH4-STP g VS-1). A good correlation have been found in this study between lignin removal and reducing sugar produced where, the total lignin removal after treated with Trichoderma sp. was 60% followed by 43% after treated with A.Fumigatus SK1.The reducing sugar produced after pre-treated with Trichoderma sp. and A.Fumigatus SK1 was about 9.59 and 4.91 μmol glucose, respectively. These results collectively suggested that CM treated with Trichoderma sp. could be a better pre-treatment method for the higher methane production in anaerobic mono-digestion process.

scholarly journals Addition of Different Biochars as Catalysts during the Mesophilic Anaerobic Digestion of Mixed Wastewater Sludge

Catalysts ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1094
Author(s):  
Marco Chiappero ◽  
Francesca Cillerai ◽  
Franco Berruti ◽  
Ondřej Mašek ◽  
Silvia Fiore
Keyword(s):  
Anaerobic Digestion ◽  
Methane Production ◽  
Biogas Production ◽  
Chemical Properties ◽  
Principal Component ◽  
Gompertz Model ◽  
Wastewater Sludge ◽  
Total Carbon ◽  
Lag Phase ◽  
Working Volume

Biochar (BC) recently gained attention as an additive for anaerobic digestion (AD). This work aims at a critical analysis of the effect of six BCs, with different physical and chemical properties, on the AD of mixed wastewater sludge at 37 °C, comparing their influence on methane production and AD kinetics. AD batch tests were performed at the laboratory scale operating 48 reactors (0.25 L working volume) for 28 days with the addition of 10 g L−1 of BC. Most reactors supplemented with BCs exhibited higher (up to 22%) methane yields than the control reactors (0.15 Nm3 kgVS−1). The modified Gompertz model provided maximum methane production rate values, and in all reactors the lag-phase was equal to zero days, indicating a good adaptation of the inoculum to the substrate. The potential correlations between BCs’ properties and AD performance were assessed using principal component analysis (PCA). The PCA results showed a reasonable correlation between methane production and the BCs’ O–C and H–C molar ratios, and volatile matter, and between biogas production and BCs’ pore volume, specific surface area, and fixed and total carbon. In conclusion, the physic-chemical properties of BC (specifically, hydrophobicity and morphology) showed a key role in improving the AD of mixed wastewater sludge.

scholarly journals Influences of Temperature and Substrate Particle Content on Granular Sludge Bed Anaerobic Digestion

2019 ◽  
Vol 10 (1) ◽  
pp. 136 ◽  
Author(s):  
Fasil Ayelegn Tassew ◽  
Wenche Hennie Bergland ◽  
Carlos Dinamarca ◽  
Rune Bakke
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Scale Up ◽  
Swine Manure ◽  
Granular Sludge ◽  
Anaerobic Sludge ◽  
Temperature Dependent ◽  
Particle Content ◽  
Comparable Amount ◽  
High Particle

Influences of temperature (25–35 °C) and substrate particulate content (3.0–9.4 g total suspended solids (TSS)/L) on granular sludge bed anaerobic digestion (AD) were analyzed in lab-scale reactors using manure as a substrate and through modeling. Two particle levels were tested using raw (RF) and centrifuged (CF) swine manure slurries, fed into a 1.3-L lab-scale up-flow anaerobic sludge bed reactor (UASB) at temperatures of 25 °C and 35 °C. Biogas production increased with temperature in both high- and low-particle-content substrates; however, the temperature effect was stronger on high-particle-content substrate. RF and CF produced a comparable amount of biogas at 25 °C, suggesting that biogas at this temperature came mainly from the digestion of small particles and soluble components present in similar quantities in both substrates. At 35 °C, RF showed significantly higher biogas production than CF, which was attributed to increased (temperature-dependent) disintegration of larger solid particulates. Anaerobic Digestion Model No.1 (ADM1) based modeling was carried out by separating particulates into fast and slow disintegrating fractions and introducing temperature-dependent disintegration constants. Simulations gave a better fit for the experimental data than the conventional ADM1 model.

Comparison of One-Phase and Two-Phase Anaerobic Digestion of Swine Manure

2013 ◽  
Vol 726-731 ◽  
pp. 2875-2880
Author(s):  
Wen Hao Liu ◽  
Shi Jie Tian ◽  
Shu Biao Wu ◽  
Xiao Qian Zhang ◽  
Chang Le Pang ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Swine Manure ◽  
Continuous Stirred Tank Reactor ◽  
Organic Loading ◽  
Two Phase ◽  
Biogas Yield ◽  
Loading Rates ◽  
Significant Difference ◽  
The One

The possible exploitation of two-phase anaerobic digestion for swine manure was investigated in this study. One-phase and two-phase anaerobic digestion process were investigated in continuous stirred-tank reactor with hydraulic retention times 15 days (hydrolysis and acidification for 3 days and methanogenic12 days in two-phase). When the organic loading rates were equal to or less than 4 g VS/L day, the biogas yield and volumetric production of two processes were no significant difference. However, in the case of organic loading rates being equal to or higher than 5.0 g VS/L day, two-phase biogas yield and volumetric biogas production averaged 0.294 L CH4/g VS added and 2.218 L/L day, compared with one-phase averaged 0.255L CH4/g VS added and 2.168 L/L day, respectively. Significant advantages in saving cost of biogas project were indicated by the comparison of biogas production and COD degradation in the one-phase and two-phase processes.

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