The Distribution and Prevalence of High-Risk HPV Genotypes Other than HPV-16 and HPV-18 among Women Attending Gynecologists’ Offices in Kazakhstan

Cervical cancer represents a great burden to public health of women. This study aimed to obtain a nationwide genotyping survey and analysis of high risk-HPV including those that are caused by HPV types other than HPV-16 and HPV-18, among women in Kazakhstan. This study was conducted based on the collection of survey and cervical swabs of 1645 women across the country. The samples were genotyped for high-risk HPV types based on real-time PCR methods. Collected data was analyzed with the focus on high-risk HPV types other than HPV-16 and -18. Infection was present in 22% of women who participated in the study. The most prevalent types were HPV-31 among single infections and HPV-68 among multiple infections. Conclusively, despite the lack of attention high-risk HPV types beyond HPV-16 and -18 get in attempts of cervical cancer prevention in Kazakhstan, their prevalence is high and plays a large role in cervical cancer epidemiological situation.

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HPV genotypes and epidemiology in women cervical cancer in Senegal.

10.21203/rs.2.15323/v4 ◽

2021 ◽

Author(s):

Khadidiatou Niane ◽

Cheikh Tidiane Diagne ◽

Gora Diop ◽

Ndongo Dia ◽

Cheikh Talla ◽

...

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Causes Of Death ◽

Sub Saharan Africa ◽

Multiple Infections ◽

Hpv 16 ◽

Hpv Genotypes ◽

Hpv Types ◽

Sub Saharan ◽

High Risk Hpv

Abstract Background Cases of cervical cancer are increasing steadily in sub-Saharan Africa, with over 75,000 new cases and nearly 50,000 deaths a year (Mboumba et al., 2017). In Senegal, pathologies such as cervical cancer are one of the top causes of death and the Human papillomavirus (HPV) is its aetiological agent (Steenbergen et al., 2005). Methods The aim of the study is to analyse the distribution of HPV among Senegalese women with cervical cancer. The main objective of this study is to identify the HPV types associated or “co-associated” with cervical oncogenesis in Senegal. The association with the risk factors of cervin carcinogenesis were analysed as well. Cervical biopsies were performed on the women admitted to Aristide Hospital Le Dantec-Julio Curie Institute. Three methods were used to detect HPV genotypes: SANGER sequencing genotyping (Applied BioSystems), PCR real-time approach technique (HPV 16 & 18 RealTime PCR kit) (www.bioneer.co.kr) and the genotyping approach from Chippron (HPV kit 3.5 LCDArray) ([email protected]).Results In this study, 24.16% of monoinfections and 75.83% of multiple infections (co-infections) were noted and the majority were at high risk (HR-HPV types). It appears that the HPV genotypes 16, 18 and 45 are the most found in tumors. The most common types of HPV in our study were HPV 16 (100%), 18 (83%), 45 (33%), 33 (31%), 59 (28%), 35 (12%), 31 (11%), 58 (8%), 39 and 73 (4%), 44, 54 and 68 (3%). In the Dakar region, which had the highest number of cases, a prevalence of 17.89% of HR-HPV co-infections was found and the majority of our patients were on a polygamous diet. Polygamy could therefore be a cofactor in the occurrence of cervical cancer in Senegalese women.Conclusion Polygamy could represent a cofactor in the occurrence of cervical cancer in Senegalese women. No association was found between high-risk HPV co-infections and cancer stages. However, an increase of our cohort would be necessary to affirm these hypotheses.

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Human Papilloma Virus Types 16/18 Distribution in Invasive Cervical Cancer: An Evidence for Vaccination in Bihar, India

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2020/v32i4831125 ◽

2021 ◽

pp. 59-68

Author(s):

Rahul Kumar ◽

Vinita Trivedi ◽

Richa Chauhan ◽

Akhtar Parwez ◽

Biplab Pal ◽

...

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Human Papilloma Virus ◽

Vaccination Program ◽

Hpv 16 ◽

Tissue Samples ◽

Papilloma Virus ◽

Cancer Tissues ◽

High Risk Hpv ◽

Hpv 18

There is high incidence of cervical cancer in Bihar, India. Vaccination for cervical cancer in developed countries has played a crucial role in limiting the incidence rate of cervical cancer worldwide. In consideration of debate on clinical efficacy of Human Papilloma Virus (HPV) vaccine in India, study on the prevalence of high risk HPV 16/18 strains in different regions of the nation becomes very crucial. Few individual states have started vaccination but centralised vaccination program does not exist due to lack of sufficient genotypic study of Human Papilloma Virus in different parts of India. Bihar is the third most populous state of India and HPV 16/18 distribution has not been reported yet. The nationwide data of HPV 16/18will help to develop a unified centralised vaccination program. We carried out a distribution study of high risk HPV type 16 and 18 in cervical cancer patients attending a tertiary care hospital of Bihar, India.HPV 16/18 types were analysed in cervical cancer tissues (n = 96) of patients attending the regional cancer hospital of Bihar. Tissue samples were analysed for HPV 16 and HPV 18 using a Real Time PCR technique. The results suggest very high prevalence of HPV 16/18. HPV was identified in all the samples (96/96). About, 74 (77.08%) samples presented with HPV 16 whereas, 16 (16.67%) of the samples presented with HPV 18. 6 Co-infection was presented in 6 (6.25%) of the samples of cervical cancer tissues. HPV 16/18 prevalence is more in the women aged between 41 to 61 years.We report 100% prevalence of HPV16/18 in the cervical cancer tissue samples. A way to minimise this gynaecological concern would be to introduce prophylactic vaccines and early screening in the state of Bihar. The data generated would be crucial in drafting for community screening of HPV. We strongly emphasize the prophylactic HPV Vaccination against HPV 16 to control the alarming rate of cervical cancer in one of the most populous state of India, Bihar.

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HPV Genotypes and Epidemiology in Women With Cervical Cancer in Senegal.

10.21203/rs.2.15323/v3 ◽

2020 ◽

Author(s):

KHADIDIATOU NIANE ◽

Cheikh Tidiane DIAGNE ◽

Gora DIOP ◽

Ndongo DIA ◽

Cheikh TALLA ◽

...

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Causes Of Death ◽

Common Type ◽

Sub Saharan Africa ◽

Multiple Infections ◽

Hpv Genotypes ◽

Hpv Types ◽

Sub Saharan ◽

High Risk Hpv

Abstract Background Cases of cervical cancer are increasing steadily in sub-Saharan Africa, with over 75,000 new cases and nearly 50,000 deaths a year (Mboumba et al., 2017). In Senegal, pathologies such as cervical cancer are one of the top causes of death and the Human papillomavirus (HPV) is its aetiological agent (Steenbergen et al., 2005). Methods The aim of the study is to analyse the distribution of HPV among Senegalese women with cervical cancer. The main objective of this study is to identify the HPV types associated or “co-associated” with cervical oncogenesis in Senegal. The correlations with the risk factors of cervin carcinogenesis were analysed as well. Cervical biopsies were performed on the women admitted to Aristide Hospital Le Dantec-Julio Curie Institute. Three methods were used to detect HPV genotypes: SANGER sequencing genotyping (Applied BioSystems), PCR real-time approach technique (HPV 16 & 18 RealTime PCR kit) (www.bioneer.co.kr) and the genotyping approach from Chippron (HPV kit 3.5 LCDArray) ([email protected]). Results In this study, the sample had multiple infections (co-infections), and a majority of the coinfections were high-risk types (HR-HPV types). The most common type of HPV in our study were 16 (34.37%), 18 (23.29%), 45 (10.75%), 33 (9.94%), 59 (9.09%), (3.97%) and 31 (3.69%). Among the co-infections detected in different regions of Senegal in women with cervical cancer, we found that HPV types 16 and 18 had the highest prevalence. In the Dakar region, which had the highest number of cases, a prevalence of 17.89% of HR-HPV co-infections was found. Conclusion Polygamy could represent a cofactor in the occurrence of cervical cancer in Senegalese women. No association was found between high-risk HPV co-infections and cancer stages. However, an increase of our cohort would be necessary to affirm these hypotheses.

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Human Papillomavirus Type Distribution and Correlation with Cyto-Histological Patterns in Women from the South of Italy

Infectious Diseases in Obstetrics and Gynecology ◽

10.1155/2009/198425 ◽

2009 ◽

Vol 2009 ◽

pp. 1-4 ◽

Cited By ~ 5

Author(s):

Paola Menegazzi ◽

Luisa Barzon ◽

Giorgio Palù ◽

Elisa Reho ◽

Luigi Tagliaferro

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Hpv Infection ◽

Multiple Infection ◽

Multiple Infections ◽

The South ◽

Hpv 16 ◽

Specific Distribution ◽

Hpv Types ◽

High Risk Hpv

Human papillomavirus (HPV) type-specific distribution was evaluated in genital samples collected from 654 women from the South of Italy undergoing voluntary screening and correlated with cyto-histological abnormalities. HPV DNA was detected in 45.9% of the samples, 41.7% of which had multiple infection and 89.0% had high-risk HPV infection. The prevalence of HPV infection and the rate of multiple infections decreased with age, suggesting natural selection of HPV types with better fitness. In line with other Italian studies, the most common HPV types were HPV-6 and HPV-16, followed by HPV-51, HPV-31, HPV-53, and HPV-66, in women with both normal and abnormal cytology. Cervical intraepithelial lesions grade 2 or 3 were associated with high-risk HPV-16, HPV-18, HPV-31, and HPV-51 infection. These data indicate that prophylactic HPV vaccination is expected to reduce the burden of HPV-related cervical lesions in this population, but also suggest the potential utility of new vaccines with larger type coverage.

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Expression of the high-risk human papillomavirus type 18 and 45 E7 oncoproteins in cervical carcinoma biopsies

Journal of General Virology ◽

10.1099/vir.0.81390-0 ◽

2005 ◽

Vol 86 (12) ◽

pp. 3235-3241 ◽

Cited By ~ 8

Author(s):

Marc Fiedler ◽

Sigrun Ressler ◽

Beatriz Campo-Fernández ◽

Andreas Laich ◽

Lars Jansen ◽

...

Keyword(s):

High Risk ◽

Cervical Carcinoma ◽

Human Papillomaviruses ◽

Hpv 16 ◽

Cervical Cancers ◽

Hpv Types ◽

High Level ◽

E7 Proteins ◽

High Risk Hpv ◽

Hpv 18

E7 proteins are major oncoproteins of high-risk human papillomaviruses (HPVs), which play a key role in cervical carcinogenesis. These proteins have been shown to immortalize primary human cells. Due to the absence of antibodies with suitable sensitivity and specificity, little is known about expression of the E7 oncoproteins in naturally infected tissues. Recently, high-level expression of the E7 protein of HPV-16, the most prevalent oncogenic HPV type, was demonstrated in cervical carcinomas by immunohistochemistry; however, approximately 15 additional high-risk HPV types are known to be associated with cervical carcinoma. It is unknown whether the E7 oncoproteins of HPV-18 and -45, the second and third most prevalent HPV types, are expressed in cervical cancers. Using antibodies against HPV-18 and -45 E7 proteins, it is shown here for the first time that the HPV-18 and -45 E7 proteins can be detected in cervical carcinoma biopsies. Together with anti-HPV-16 E7 antibodies, this could create the possibility of detecting E7 oncoproteins in approximately 80 % of all cervical cancers.

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High-Risk Human Papillomavirus E6 Oncoproteins Interact with 14-3-3ζ in a PDZ Binding Motif-Dependent Manner

Journal of Virology ◽

10.1128/jvi.02074-12 ◽

2012 ◽

Vol 87 (3) ◽

pp. 1586-1595 ◽

Cited By ~ 45

Author(s):

Siaw Shi Boon ◽

Lawrence Banks

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

High Risk ◽

Pdz Domain ◽

Binding Motif ◽

Dependent Manner ◽

Hpv E6 ◽

Hpv Types ◽

High Risk Hpv ◽

Hpv 18

ABSTRACTCervical cancer develops through the combined activities of the human papillomavirus (HPV) E6 and E7 oncoproteins. A defining characteristic of E6 oncoproteins derived from cancer-causing HPV types is the presence of a PDZ binding motif (PBM) at the extreme carboxy terminus of the protein which is absent from E6 proteins derived from the so-called low-risk HPV types. Within this PBM is also a protein kinase A (PKA) phospho-acceptor site, which is thought to negatively regulate the association of E6 with its PDZ domain-containing substrates. We can now show that phosphorylation of E6 by PKA and/or AKT confers the ability to interact with 14-3-3ζ. The interaction is direct and specific for the high-risk HPV E6 oncoproteins, although there are significant differences in the efficiencies with which HPV-16, HPV-18, and HPV-31 E6 oncoproteins can associate with 14-3-3ζ; this correlates directly with their respective susceptibilities to phosphorylation by PKA and/or AKT. We demonstrate here that the interaction between E6 and 14-3-3ζ also requires integrity of the E6 PBM, and downregulation of 14-3-3ζ results in a marked reduction in the levels of HPV-18 E6 expression in HeLa cells. Using phospho-specific anti-E6 antibodies, we also demonstrate significant levels of E6 phosphorylationin vivo. These studies redefine the potential relevance of the E6 PBM in the development of cervical cancer, suggesting that interaction with 14-3-3ζ, as well as the more well-established interactions with PDZ domain-containing substrates, is likely to be responsible for the biological activities attributed to this region of the high-risk HPV E6 oncoproteins.

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Human papillomavirus genotype distribution in Ethiopia: an updated systematic review

Virology Journal ◽

10.1186/s12985-022-01741-1 ◽

2022 ◽

Vol 19 (1) ◽

Author(s):

Awoke Derbie ◽

Daniel Mekonnen ◽

Endalkachew Nibret ◽

Melanie Maier ◽

Yimtubezinash Woldeamanuel ◽

...

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Human Papillomaviruses ◽

Low Risk ◽

Scientific Quality ◽

Genotype Distribution ◽

Hpv 16 ◽

Hpv Genotypes ◽

Hpv Types ◽

Hpv 18

Abstract Background Cervical cancer is caused by infection with high-risk human papillomaviruses (HR-HPVs). It is one of the leading causes of cancer-related deaths in Ethiopia and globally. To develop efficient vaccination and HPV-based cervical cancer screening approaches, data on genotype distribution of HPVs is crucial. Hence, the study was aimed to review HPV genotype distribution in Ethiopia. Methods Research articles were systematically searched using comprehensive search strings from PubMed/Medline and SCOPUS. Besides, Google Scholar was searched manually for grey literature. The last search was conducted on 18 August 2021. The first two authors independently appraised the studies for scientific quality and extracted the data using Excel sheet. The pooled HPV genotype distribution was presented with descriptive statistics. Results We have included ten studies that were reported from different parts of the country during 2005 and 2019. These studies included 3633 women presented with different kinds of cervical abnormalities, from whom 29 different HPV genotypes with a sum of 1926 sequences were reported. The proportion of high-risk, possible/probable high-risk and low-risk HPVs were at 1493 (77.5%), 182 (9.4%) and 195 (10.1%), respectively. Of the reported genotypes, the top five were HPV 16 (37.3%; 95% CI 35.2.1–39.5%), HPV 52 (6.8%; 95% CI 5.8–8.0%), HPV 35 (4.8%; 95% CI 3.9–5.8%), HPV 18 (4.4%; 95% CI 3.5–5.3%) and HPV 56 (3.9%: 95% CI 3.1–4.9%). Some of other HR-HPV groups include HPV 31 (3.8%), HPV 45 (3.5%), HPV 58 (3.1%), HPV 59(2.3%), and HPV 68 (2.3%). Among the high-risk types, the combined prevalence of HPV 16/18 was at 53.7% (95% CI 51.2–56.3%). HPV 11 (2.7%: 95% CI 2.1–3.5%), HPV 42 (2.1%: 95% CI 1.5–2.8%) and HPV 6 (2.1%: 95% CI 1.4–2.7%) were the most common low-risk HPV types. Conclusions We noted that the proportion of HR-HPV types was higher and HPV 16 in particular, but also HPV 52, HPV 35 and HPV 18, warrant special attention in Ethiopian’s vaccination and HPV based cervical screening program. Additional data from other parts of the country where there is no previous HPV genotype report are needed to better map the national HPV genotypes distribution of Ethiopia.

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Distribution of high risk Human Papilloma Virus genotypes among cervical cancer patients in a tertiary level hospital in Bangladesh

Bangladesh Medical Research Council Bulletin ◽

10.3329/bmrcb.v45i2.42536 ◽

2019 ◽

Vol 45 (2) ◽

pp. 86-92

Author(s):

Munira Jahan ◽

Tania Islam ◽

Sharmin Sultana ◽

Monira Pervin ◽

Prof. Ashrafunnessa ◽

...

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Human Papilloma Virus ◽

Cancer Patients ◽

Hpv Infection ◽

Multiple Infections ◽

Hpv 16 ◽

Papilloma Virus ◽

Hpv Dna ◽

Hpv 18

Background: Cervical cancer is one of the most common types of cancer affecting women worldwide. Persistent HPV infection plays a major role in cervical cancer. The risk of cervical cancer has increased in parallel with the incidence of certain genotypes of human papilloma virus (HPV). Methods: The study was aimed to detect the distribution of HPV genotypes among cervical cancer patients from a specialized hospital in Dhaka, Bangladesh. HPV DNA testing was done by polymerase chain reaction (PCR) using SPF-10 broad-spectrum primers followed by genotyping by reverse hybridization using the INNO-LIPA genotyping system at the Department of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka. Results: HPV 16 was more prevalent (72.0%) in cervical cancer patients from Bangladesh followed by type 18 (6.0%) and 45 (2.0%). Genotype 16 and 18 alone and as co-infection were detected in 88.0% cases. Multiple HPV infection was found in 20% patients. Along with high risk (HR) HPV 16, 18 and 45 HR HPV 56, 39, 31 and 58 were also prevalent in multiple infections. Conclusion: Thus the study concluded that HR HPV 16 and HPV 18 were more prevalent genotypes among cervical cancer patients in a specialised hospital in Bangladesh. Along with HR HPV 16 and HPV 18, HR HPV 45, 56, 39, 31 and 58 were also prevalent Bangladesh Med Res Counc Bull 2019; 45: 86-92

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APPLICATION OF REAL-TIME PRC TECHNIQUE AND REVERSE DOT-BLOT FOR DETECTION THE HIGH RISK TYPES OF HUMAN PAPILLOMAVIRUS CAUSING CERVICAL CANCER

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2017.3.15 ◽

2017 ◽

pp. 99-103

Author(s):

Van Bao Thang Phan ◽

Hoang Bach Nguyen ◽

Van Thanh Nguyen ◽

Thi Nhu Hoa Tran ◽

Viet Quynh Tram Ngo

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Real Time ◽

Real Time Pcr ◽

Dot Blot ◽

The Real ◽

Dot Blot Assay ◽

Hpv Types ◽

High Risk Hpv ◽

Reverse Dot Blot

Introduction: Infection with HPV is the main cause of cervical cancer. Determining HPV infection and the types of HPV plays an important role in diagnosis, treatment and prognosis of cervicitis/cervical cancer. Aims: Determining proportion of high-risk HPV types and the occurrence of coinfection with multiple HPV types. Methods: 177 women with cervicitis or abnormal Pap smear result were enrolled in the study. Performing the real-time PCR for detecting HPV and the reverse DOT-BLOT assay for determining type of HPV in cases of positive PCR. Results: 7 types of high-risk HPV was dectected, the majority of these types were HPV type 18 (74.6%) and HPV type 16 (37.6%); the proportion of infection with only one type of HPV was 30.4% and coinfection with multiple HPV types was higher (69.6%), the coinfected cases with 2 and 3 types were dominated (32.2% and 20.3%, respectively) and the coinfected cases with 4 and 5 types were rare. Conclusion: Use of the real-time PCR and reverse DOT-BLOT assay can determine the high-risk HPV types and the occurrence of coinfection with multiple HPV types. Key words: HPV type, Reverse DOT-BLOT, real-time PCR,PCR, cervical cancer

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