scholarly journals Human Papilloma Virus Types 16/18 Distribution in Invasive Cervical Cancer: An Evidence for Vaccination in Bihar, India

2021 ◽  
pp. 59-68
Author(s):  
Rahul Kumar ◽  
Vinita Trivedi ◽  
Richa Chauhan ◽  
Akhtar Parwez ◽  
Biplab Pal ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Vaccination Program ◽  
Hpv 16 ◽  
Tissue Samples ◽  
Papilloma Virus ◽  
Cancer Tissues ◽  
High Risk Hpv ◽  
Hpv 18

There is high incidence of cervical cancer in Bihar, India. Vaccination for cervical cancer in developed countries has played a crucial role in limiting the incidence rate of cervical cancer worldwide. In consideration of debate on clinical efficacy of Human Papilloma Virus (HPV) vaccine in India, study on the prevalence of high risk HPV 16/18 strains in different regions of the nation becomes very crucial. Few individual states have started vaccination but centralised vaccination program does not exist due to lack of sufficient genotypic study of Human Papilloma Virus in different parts of India. Bihar is the third most populous state of India and HPV 16/18 distribution has not been reported yet. The nationwide data of HPV 16/18will help to develop a unified centralised vaccination program. We carried out a distribution study of high risk HPV type 16 and 18 in cervical cancer patients attending a tertiary care hospital of Bihar, India.HPV 16/18 types were analysed in cervical cancer tissues (n = 96) of patients attending the regional cancer hospital of Bihar. Tissue samples were analysed for HPV 16 and HPV 18 using a Real Time PCR technique. The results suggest very high prevalence of HPV 16/18. HPV was identified in all the samples (96/96). About, 74 (77.08%) samples presented with HPV 16 whereas, 16 (16.67%) of the samples presented with HPV 18. 6 Co-infection was presented in 6 (6.25%) of the samples of cervical cancer tissues. HPV 16/18 prevalence is more in the women aged between 41 to 61 years.We report 100% prevalence of HPV16/18 in the cervical cancer tissue samples. A way to minimise this gynaecological concern would be to introduce prophylactic vaccines and early screening in the state of Bihar. The data generated would be crucial in drafting for community screening of HPV. We strongly emphasize the prophylactic HPV Vaccination against HPV 16 to control the alarming rate of cervical cancer in one of the most populous state of India, Bihar.

scholarly journals Distribution of high risk Human Papilloma Virus genotypes among cervical cancer patients in a tertiary level hospital in Bangladesh

2019 ◽  
Vol 45 (2) ◽  
pp. 86-92
Author(s):  
Munira Jahan ◽  
Tania Islam ◽  
Sharmin Sultana ◽  
Monira Pervin ◽  
Prof. Ashrafunnessa ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Cancer Patients ◽  
Hpv Infection ◽  
Multiple Infections ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Hpv 18

Background: Cervical cancer is one of the most common types of cancer affecting women worldwide. Persistent HPV infection plays a major role in cervical cancer. The risk of cervical cancer has increased in parallel with the incidence of certain genotypes of human papilloma virus (HPV). Methods: The study was aimed to detect the distribution of HPV genotypes among cervical cancer patients from a specialized hospital in Dhaka, Bangladesh. HPV DNA testing was done by polymerase chain reaction (PCR) using SPF-10 broad-spectrum primers followed by genotyping by reverse hybridization using the INNO-LIPA genotyping system at the Department of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka. Results: HPV 16 was more prevalent (72.0%) in cervical cancer patients from Bangladesh followed by type 18 (6.0%) and 45 (2.0%). Genotype 16 and 18 alone and as co-infection were detected in 88.0% cases. Multiple HPV infection was found in 20% patients. Along with high risk (HR) HPV 16, 18 and 45 HR HPV 56, 39, 31 and 58 were also prevalent in multiple infections. Conclusion: Thus the study concluded that HR HPV 16 and HPV 18 were more prevalent genotypes among cervical cancer patients in a specialised hospital in Bangladesh. Along with HR HPV 16 and HPV 18, HR HPV 45, 56, 39, 31 and 58 were also prevalent Bangladesh Med Res Counc Bull 2019; 45: 86-92

scholarly journals Μελέτη της λοίμωξης από τους ιούς Epstein-Barr (EBV), ΒΚ (BKV) και του ιού του θηλώματος (HPV) στον θυρεοειδή αδένα και ο ρόλος της στην ανάπτυξη καρκίνου του θυρεοειδούς

2015 ◽  
Author(s):  
Δημήτριος Σταματίου
Keyword(s):  
High Risk ◽  
Human Papilloma Virus ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Epstein Barr ◽  
Hpv 18

Παρά το γεγονός ότι οι ιοί έχουν πρόσφατα εμπλακεί στη ρύθμιση της επιθηλιο-μεσεγχυματικής μετάβασης και στην πρόοδο της καρκινογένεσης, πολύ λίγα είναι γνωστά σχετικά με τη συμμετοχή των ιογενών λοιμώξεων στις κακοήθειες του θυρεοειδούς αδένα. Σκοπός της παρούσας μελέτης ήταν η ανίχνευση αλληλουχιών τριών δυνητικά καρκινογόνων ιών, του BKV, του EBV και του HPV σε μια σειρά δειγμάτων θυρεοειδεκτομής.Ο ιός Epstein-Barr (EBV) ανήκει στην ομάδα των ερπητοϊών και προκαλεί τη λοιμώδη μονοπυρήνωση. Έχει επίσης αναγνωριστεί ως παράγοντας στην ανάπτυξη διαφόρων νεοπλασιών, όπως το λέμφωμα Burkitt, το ρινοφαρυγγικό καρκίνωμα, και άλλες λεμφοβλαστικές διαταραχές σε ανοσοκατεσταλμένα άτομα, γεγονός που υποδεικνύει ότι ο EBV πιθανότατα παίζει σημαντικό ρόλο στη διαδικασία της καρκινογένεσης. Όσον αφορά το θυρεοειδή αδένα, υπάρχουν αρκετές ενδείξεις ότι ο EBV σχετίζεται με την ανάπτυξη κακοήθους λεμφώματος του θυρεοειδούς αλλά και με την εξαλλαγή του διαφοροποιημένου θυρεοειδικού καρκινώματος στην πιο κακοήθη αδιαφοροποίητη μορφή του. Ο ιός BK (BKV) ανήκει στο γένος των polyoma ιών και προκαλεί αιμορραγική κυστίτιδα σε ανοσοκατεσταλμένους ασθενείς, ενώ έχει ανιχνευθεί σ’ένα σημαντικό αριθμό κακοηθειών. Ο ιός Simian Virus 40 (SV40), ένα άλλο μέλος της οικογένειας των polyoma ιών, ξενιστής του οποίου είναι ο ασιατικός ρέζους πίθηκος, έχει εμπλακεί στην καρκινογένεση σε τρωκτικά, ενώ έχει ανιχνευθεί σε δείγματα τόσο θυρεοειδικού καρκίνου, όσο και σε καλοήθη θυρεοειδικά οζίδια.Ο ιός του θηλώματος (Human Papilloma Virus-HPV) ανήκει στο γένος των papilloma ιών. Υπάρχουν πολλοί τύποι HPV, οι οποίοι, εκτός από το ότι προκαλούν διαφόρων ειδών θηλώματα σε διαφορετικά σημεία του σώματος, ανάλογα με τον τύπο, έχει βρεθεί ότι σχετίζονται ιδιαίτερα με την ανάπτυξη καρκίνων. Οι τύποι HPV-16 και HPV-18 χαρακτηρίζονται ως «υψηλού κινδύνου» (high-risk) όσον αφορά την καρκινογένεση, κυρίως του τραχήλου της μήτρας. Μια πρόσφατη έρευνα έδειξε ότι ο HPV-18 έχει τη δυνατότητα να αλληλεπιδρά με πυρηνικούς υποδοχείς, όπως τους υποδοχείς της θυρεοειδικής ορμόνης, των οιστρογόνων και των ανδρογόνων και να τους ενεργοποιεί ανεξαρτήτως της παρουσίας ή της απουσίας της αντίστοιχης ορμόνης. Η μελέτη περιελάμβανε παρασκευάσματα θυρεοειδεκτομής από τριάντα ασθενείς με οζίδια θυρεοειδούς αδένα μέσα σε μια περίοδο 3 ετών. Η δειγματοληψία περιελάμβανε τόσο οζιδιδιακό, όσο και παρακείμενο φυσιολογικό ιστό από το παρασκεύασμα κάθε ασθενούς. Αλληλουχίες των ιϊκών γονιδίων του BKV (γονίδιο VP1), του EBV (γονίδια LMP1, EBNA2 και EBER1) και του HPV (γονίδιο L1), ενισχύθηκαν με PCR. Τα αποτελέσματα της PCR επιβαιώθηκαν με ανάλυση direct sequencing.Αλληλουχίες του γονιδίου VP1 ανιχνεύθηκαν στο 60% (18/30) των δειγμάτων θυρεοειδικού καρκίνου/πολυοζώδους υπερπλασίας, σε σύγκριση με το 43.3% (13/30) σε αντίστοιχο παρακείμενο φυσιολογικό ιστό. Σε δεκαπέντε από τα τριάντα (50%) δείγματα θυρεοειδικού καρκίνου/πολυοζώδους υπερπλασίας, αναδείχθηκαν αλληλουχίες του γονιδίου LMP1 σε σύγκριση με 46.7% (14/30) σε παρακείμενο φυσιολογικό ιστό. Αλληλουχίες του γονιδίου EBNA2 ανιχνεύθηκαν στο 90% (27/30) των δειγμάτων θυρεοειδικού καρκίνου/πολυοζώδους υπερπλασίας, ενώ στο ίδιο ακριβώς ποσοστό (90%-27/30), ανιχνεύθηκαν και στους αντίστοιχους παρακείμενους φυσιολογικούς ιστούς. Όλα τα δείγματα ήταν αρνητικά για αλληλουχίες του EBER1, καθώς και για DNA του ιού HPV.Η παρούσα μελέτη συνιστά τη “λοίμωξη” από BKV και EBV σαν ένα πρώιμο γεγονός, που προφανώς συμβαίνει σε φυσιολογικό ιστό. Οι αλληλουχίες του LMP1, παρατηρήθηκε ότι ανιχνεύθηκαν συχνότερα στα κακοήθη δείγματα συγκριτικά με τα δείγματα φυσιολογικού ιστού. Άν και τα ευρήματά μας δέν επιτρέπουν την εξαγωγή συμπερασμάτων σχετικά με το δυνητικά ογκογόνο ρόλο των εξετασθέντων ιών ως προς το θυρεοειδή αδένα, συνιστούν ένα μοτίβο συνύπαρξης, ή “ενδημικότητας”. Καθώς ο αριθμός των εξετασθέντων δειγμάτων ήταν μικρός και όλα τα δείγματα προέρχονταν από το ίδιο νοσοκομείο, απαιτούνται περαιτέρω μελέτες ώστε να εκτιμηθούν αυτές οι παρατηρήσεις σε σχέση με τοπικοπεριοχικά χαρακτηριστικά.

scholarly journals Results of the first human papilloma virus center in Hungary (2007–2011)

2011 ◽  
Vol 152 (45) ◽  
pp. 1804-1807
Author(s):  
Ádám Galamb ◽  
Attila Pajor ◽  
Zoltán Langmár ◽  
Gábor Sobel
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Hpv Testing ◽  
Papilloma Virus ◽  
Cytological Atypia ◽  
Hpv Dna ◽  
International Data ◽  
Hpv Types ◽  
High Risk Hpv

Human papilloma virus (HPV) is the most common sexually transmitted infection in the 21st century. It has been established that infections with specific HPV types are contributing factors to cervical cancer. Approximately 99.7% of cervical cancers are associated with high risk HPV types. HPV testing plays an important role in the prevention, by decreasing the prevalence and the mortality of cervical cancer. There are 16 HPV-centers operating in Hungary, in which patients undergo HPV screening, cervical exams, and treatment based on standardized guidelines. Patients and methods: The first HPV-center was founded in 2007 in Budapest, at the 2nd Department of Obstetrics and Gynecology, Semmelweis University. This study aimed to define the presence and prevalence of HPV-DNA in the cervical swab samples obtained from patients in our center. Authors conducted to assess the age-specific-prevalence, and HPV type distribution, the associated cervical abnormalities, comparing our results with international data. Results: Overall 1155 woman underwent HPV-testing and genotyping, using polymerase chain reaction. Overall, 55.5% of patients had positive test for HPV DNA types, in which 38.5% for high-risk HPV DNA. Overall prevalence was the highest among females aged 15 to 25years (62.9%). The most common HPV type found was the high risk type 16 (19.5% among the patients with positive HPV testing). Presence of high risk HPV with concurrent cervical cytological abnormality was in 32%. More than two-thirds of woman with cytological atypia (70.6%) were infected with two or more high risk HPV types. HPV 16 was detected in 32% of patients with cytological abnormalities. Conclusions: The results suggest that the prevalence of HPV in this study population exceeds the international data. The results attracts the attention the peak prevalence of the high risk types in the youngest age-group, and the higher risk of cervical abnormality in case of presence of two or more HPV types. The dominance of type 16 and 18 was predictable, but the strong attendance of type 51 and 31 among patients who had cytological atypia, was slightly surprising. Orv. Hetil., 2011, 152, 1804–1807.

scholarly journals The Distribution and Prevalence of High-Risk HPV Genotypes Other than HPV-16 and HPV-18 among Women Attending Gynecologists’ Offices in Kazakhstan

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 794
Author(s):  
Gulzhanat Aimagambetova ◽  
Aisha Babi ◽  
Alpamys Issanov ◽  
Sholpan Akhanova ◽  
Natalya Udalova ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Cervical Cancer Prevention ◽  
Multiple Infections ◽  
Hpv 16 ◽  
Hpv Types ◽  
High Risk Hpv ◽  
Hpv 18 ◽  
Great Burden ◽  
Epidemiological Situation

Cervical cancer represents a great burden to public health of women. This study aimed to obtain a nationwide genotyping survey and analysis of high risk-HPV including those that are caused by HPV types other than HPV-16 and HPV-18, among women in Kazakhstan. This study was conducted based on the collection of survey and cervical swabs of 1645 women across the country. The samples were genotyped for high-risk HPV types based on real-time PCR methods. Collected data was analyzed with the focus on high-risk HPV types other than HPV-16 and -18. Infection was present in 22% of women who participated in the study. The most prevalent types were HPV-31 among single infections and HPV-68 among multiple infections. Conclusively, despite the lack of attention high-risk HPV types beyond HPV-16 and -18 get in attempts of cervical cancer prevention in Kazakhstan, their prevalence is high and plays a large role in cervical cancer epidemiological situation.

scholarly journals Distribution of Human papilloma virus genotypes in cervical cancer tissues

2014 ◽  
Vol 66 (2) ◽  
pp. 573-577
Author(s):  
M. Stamenkovic ◽  
Aleksandra Knezevic ◽  
I. Kuzmanovic ◽  
Danijela Karalic ◽  
Tanja Jovanovic
Keyword(s):  
Cervical Cancer ◽  
Human Papilloma Virus ◽  
Direct Sequencing ◽  
Genotype Distribution ◽  
National Guidelines ◽  
Tissue Samples ◽  
Papilloma Virus ◽  
Incidence And Mortality ◽  
Hpv Types ◽  
Cancer Tissues

Cervical cancer incidence and mortality rates in Serbia are among the highest in Europe and data on Human papilloma virus (HPV) type distribution are scarce. The aim of this study was to determine the prevalence of HPV types in archival specimens of cervical cancer tissues of women in the Serbian population. A total of 45 paraffin-embedded tissue samples of cervical carcinoma were used in this study. The procedure included deparaffinization of tissue samples, DNA extraction, PCR, gel electrophoresis and HPV genotyping by direct sequencing. HPV was detected in 32 samples (71%). Genotyping revealed the presence of 6 high-risk HPV types 16, 18, 33, 45, 53 and 58, where HPV type 16 was the most prevalent type (73.7%). The results of this study and further studies will provide more detailed information about HPV genotype distribution and may contribute to the formulation of national guidelines for the prevention of cervical cancer.

scholarly journals High-risk human papilloma virus infection and cervical neoplasm in female inflammatory bowel disease patients: a cross-sectional study

2019 ◽  
Vol 7 (5) ◽  
pp. 338-344
Author(s):  
Miao Li ◽  
Qing-Fan Yang ◽  
Qian Cao ◽  
Jian Tang ◽  
Yu Gao ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Inflammatory Bowel ◽  
High Risk Hpv

Abstract Background and aim This cross-sectional study investigated the prevalence and risk factors of high-risk human papilloma virus (HPV) infection, especially types 16 and 18, and cervical neoplasia in female Inflammatory bowel disease (IBD) patients. Methods From July 2014 to January 2017, sexually active, female, Chinese IBD patients (21–60 years) and age-matched controls underwent cervical ThinPrep cytology testing (TCT) and high-risk HPV-DNA detection, and completed questionnaires about awareness of cervical cancer and HPV. Cervical dysplasia was categorized as cervical intraepithelial neoplasia (CIN) 1, 2 and 3. Results Of 124 IBD patients (30 ulcerative colitis and 94 Crohn’s disease), 17 (13.7%) had high-risk HPV among whom 9 (7.3%) had HPV 16/18 infection and 4 (3.2%) had cervical CIN (3 CIN 3, 1 CIN 1) by pathology. Among 372 controls, 33 (8.9%) had high-risk HPV and only 1 (0.3%) had HPV 16 infection. Cervical TCT detected atypical squamous cells of unknown significance in one control; no control had CIN. The HPV 16/18 infection rate and CIN prevalence were significantly higher in IBD patients than controls (both P < 0.001). The HPV-infection rate was higher in patients administered methotrexate [P = 0.005, odds ratio (95% confidence interval) 4.76 (1.471–15.402)] or more than two immunosuppressants [P = 0.013, odds ratio (95% confidence interval) 3.64 (1.255–10.562)]. Thiopurine, steroid, infliximab and disease behavior/location were not associated with HPV infection. Only 29.3% of patients had undergone cervical-cancer screening. Awareness of HPV infection and HPV-related cervical cancer was poor (28.2%). Conclusions Female IBD patients are at increased risk of high-risk HPV infection and cervical neoplasia, which may be associated with immunosuppressants. Education and routine follow-up with HPV-DNA testing and TCT are recommended, especially in female Chinese IBD patients.

scholarly journals Persistent High-Risk HPV Infection and Molecular Changes Related to the Development of Cervical Cancer

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Pablo Moreno-Acosta ◽  
Alfredo Romero-Rojas ◽  
Nicolas Vial ◽  
Antonio Huertas ◽  
Jinneth Acosta ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Invasive Cervical Cancer ◽  
Transition Process ◽  
Hpv Infection ◽  
Low Grade ◽  
Preneoplastic Lesions ◽  
Hpv 16 ◽  
Molecular Changes ◽  
High Risk Hpv

This article is a preliminary investigational study that is aimed at giving hints about the interesting biomarkers involved in the transition process from low-grade cervix lesion to invasive cervical cancer. Our study focuses on the risk factors and tumour molecular changes in one patient. First in 1986, she was diagnosed a preinvasive cervix lesion. Then, 16 years later, she was diagnosed an invasive cervical cancer. The 2002 diagnosis was a squamous cell carcinoma of the cervix, stage IIIB (FIGO), whereas in 1986, she had been diagnosed a high-grade squamous intraepithelial cervical lesion. Retrospectively, the analysis of samples of preneoplastic lesions and invasive cervical cancer confirmed the histopathological diagnoses and detected the presence of HPV type and HPV-16 variants, as well as the overexpression of proteins such as hTERT, IGF1Rα, IGF1Rβ, CAIX, and GLUT1. Finally, the Arg72Pro polymorphism was detected in TP53. The role of high-risk HPV and HPV-16 variants and of hTERT, IGF1Rα, IGF1Rβ, CAIX, and GLUT1 variations seemed confirmed in the development and progression of cervical cancer. As a result, analyzing the molecular changes in one and same tumour that progresses from a low-grade cervix lesion to invasive cervical cancer could provide valuable information in order to improve detection, diagnosis, and treatment in the future.

Genetic variation of high-risk HPV-16 from a cervical cancer biopsy collected in Pakistan

2016 ◽  
Vol 11 (1) ◽  
pp. 31-38
Author(s):  
Abida Siddiqa ◽  
Muhammad Faraz Bhatti
Keyword(s):  
Cervical Cancer ◽  
Genetic Variation ◽  
High Risk ◽  
Hpv 16 ◽  
Cancer Biopsy ◽  
High Risk Hpv
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