scholarly journals Temperature and Concentration Dependence of Human Whole Blood and Protein Drying Droplets

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 231 ◽  
Author(s):  
Anusuya Pal ◽  
Amalesh Gope ◽  
Germano Iannacchione

The drying of bio-colloidal droplets can be used in many medical and forensic applications. The whole human blood is the most complex bio-colloid system, whereas bovine serum albumin (BSA) is the simplest. This paper focuses on the drying characteristics and the final morphology of these two bio-colloids. The experiments were conducted by varying their initial concentrations, and the solutions were dried under various controlled substrate temperatures using optical and scanning electron microscopy. The droplet parameters (the contact angle, the fluid front, and the first-order image statistics) reveal the drying process’s unique features. Interestingly, both BSA and blood drying droplets’ contact angle measurements show evidence of a concentration-driven transition as the behavior changes from non-monotonic to monotonic decrease. This result indicates that this transition behavior is not limited to multi-component bio-colloid (blood) only, but may be a phenomenon of a bio-colloidal solution containing a large number of interacting components. The high dilution of blood behaves like the BSA solution. The ring-like deposition, the crack morphology, and the microstructures suggest that the components have enough time to segregate and deposit onto the substrate under ambient conditions. However, there is insufficient time for evaporative-driven segregation to occur at elevated temperatures, as expected.

2012 ◽  
Author(s):  
Narjes Shojaikaveh ◽  
Cas Berentsen ◽  
Susanne Eva Johanne Rudolph-Floter ◽  
Karl Heinz Wolf ◽  
William Richard Rossen

2007 ◽  
Vol 330-332 ◽  
pp. 877-880 ◽  
Author(s):  
E.S. Thian ◽  
J. Huang ◽  
Serena Best ◽  
Zoe H. Barber ◽  
William Bonfield

Crystalline hydroxyapatite (HA) and 0.8 wt.% silicon-substituted HA (SiHA) thin films were produced using magnetron co-sputtering. These films were subjected to contact angle measurements and in vitro cell culture study using human osteoblast-like (HOB) cells. A wettability study showed that SiHA has a lower contact angle, and thus is more hydrophilic in nature, as compared to HA. Consequently, enhanced cell growth was observed on SiHA at all time-points. Furthermore, distinct and well-developed actin filaments could be seen within HOB cells on SiHA. Thus, this work demonstrated that the surface properties of the coating may be modified by the substitution of Si into the HA structure.


1992 ◽  
Vol 73 (2-3) ◽  
pp. 163-172 ◽  
Author(s):  
Dietmar Flösch ◽  
Giorgio Clarotti ◽  
Kurt E. Geckeler ◽  
François Schué ◽  
Wolfgang Göpel

2016 ◽  
Vol 869 ◽  
pp. 913-917 ◽  
Author(s):  
Ana Lucia do Amaral Escada ◽  
Javier Andres Muñoz Chaves ◽  
Ana Paula Rosifini Alves Claro

The purpose of this study was to evaluate the TiO2 nanotubes growth and the variation in its diameter to improve the surface properties of Ti-7.5Mo to use for biomedical applications. For the nanotubes TiO2 growth, the samples were anodized in glycerol and ammonium fluoride and divided according to the anodizing potential at 5V to 10V and 24 hour time. The surfaces were examined by scanning electron microscope (SEM), X-ray analysis (XRD) and contact angle measurements. The average tube diameter, ranging in size from 13 to 23 nm, was found to increase with increasing anodizing voltage. It was also observed a decrease in contact angle in accordance with the increase in the anodizing potential. The X-ray analysis showed the presence of anatase phase in samples whose potential was 10V and this condition represents a simple surface treatment for Ti-7.5Mo alloy that has high potential for biomedical applications.


2009 ◽  
Vol 255 (9) ◽  
pp. 4873-4879 ◽  
Author(s):  
Sameer Bargir ◽  
Steve Dunn ◽  
Bruce Jefferson ◽  
Jitka Macadam ◽  
Simon Parsons

Sign in / Sign up

Export Citation Format

Share Document