scholarly journals Differential Regulation of Circulating Soluble Receptor for Advanced Glycation End Products (sRAGEs) and Its Ligands S100A8/A9 Four Weeks Post an Exercise Intervention in a Cohort of Young Army Recruits

Biomolecules ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1354
Author(s):  
Ioannis-Alexandros Drosatos ◽  
James N. Tsoporis ◽  
Shehla Izhar ◽  
Sahil Gupta ◽  
George Tsirebolos ◽  
...  
Keyword(s):  
Physical Activity ◽  
Advanced Glycation End Products ◽  
Exercise Intervention ◽  
Training Period ◽  
Advanced Glycation ◽  
Direct Stimulation ◽  
Glycation End Products ◽  
End Products ◽  
Anti Inflammatory ◽  
Army Recruits

Apart from its beneficial effects on cardiovascular risk factors, an anti-inflammatory effect of exercise is strongly implicated. Yet, data regarding the effect of an exercise intervention on healthy individuals are limited and contradictory. The present study aimed to investigate the effects of a physical activity intervention on the soluble form of the receptor for advanced glycation end products (sRAGEs) and its ligands S100A8/A9. A total of 332 young army recruits volunteered and 169 completed the study. The participants underwent the standard basic training of Greek army recruits. IL-6, IL-1β, S100A8/A9, and sRAGEs were measured at the beginning and at the end of the training period. Primary rodent adult aortic smooth muscle cells (ASMCs) were analyzed for responsiveness to direct stimulation with S100A8/A9 alone or in combination with sRAGEs. At the end of the training period, we observed a statistically significant reduction in S100A8/A9 (630.98 vs. 472.12 ng/mL, p = 0.001), IL-1β (9.39 [3.8, 44.14] vs. 5.03 [2.44, 27.3] vs. pg/mL, p = 0.001), and sRAGEs (398.38 vs. 220.1 pg/mL, p = 0.001). IL-6 values did not change significantly after exercise. S100A8/A9 reduction was positively correlated with body weight (r = 0.236 [0.095, 0.370], p = 0.002) and BMI (r = 0.221 [0.092, 0.346], p = 0.004). Direct stimulation of ASMCs with S100A8/A9 increased the expression of IL-6, IL-1β, and TNF-α and, in the presence of sRAGEs, demonstrated a dose-dependent inhibition. A 4-week military training resulted in significant reduction in the pro-inflammatory cytokines IL-1β and S100A8/A9 complex. The observed reduction in sRAGEs may possibly reflect diminished RAGE axis activation. Altogether, our findings support the anti-inflammatory properties of physical activity.

scholarly journals DJC Suppresses Advanced Glycation End Products-Induced JAK-STAT Signaling and ROS in Mesangial Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Min Sun ◽  
Yan Li ◽  
Wenjie Bu ◽  
Jindong Zhao ◽  
Jianliang Zhu ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Advanced Glycation End Products ◽  
Oxidase Activity ◽  
Mesangial Cells ◽  
Dependent Manner ◽  
Advanced Glycation ◽  
Nadph Oxidase Activity ◽  
Glycation End Products ◽  
End Products ◽  
Anti Inflammatory

The antidiabetic properties and anti-inflammatory effects of Danzhi Jiangtang Capsules (DJC) have been demonstrated in clinical and laboratory experiments. In this study, we explored whether DJC can ameliorate advanced glycation end products- (AGEs-) mediated cell injury and the precise mechanisms of DJC in treating diabetic nephropathy (DN). Western blot analysis was employed to assess the expressions of iNOS, COX2, and SOCS and the phosphorylation of JAK2, STAT1, and STAT3 in glomerular mesangial cells (GMCs) after treatment with DJC. TNF-α, IL-6, and MCP-1 were determined using double-antibody sandwich ELISA. ROS and NADPH oxidase activity were measured by DCFH-DA assay and lucigenin-enhanced chemiluminescence, respectively. DJC significantly reversed the AGEs-induced expression of COX2 and iNOS. Moreover, DJC inhibited the AGEs-induced JAK2-STAT1/STAT3 activation, resulting in the inhibition of inflammatory cytokines such as IL-6, MCP-1, and TNF-αin a concentration-dependent manner. The ability of DJC to suppress STAT activation was also verified by the observation that DJC significantly increased the SOCS3 protein level. DJC reversed the AGEs-induced accumulation of ROS and NADPH oxidase activity, thus confirming that DJC possesses antioxidant activity. The results suggest that the anti-inflammatory effects of DJC in GMCs may be due to its ability to suppress the JAK2-STAT1/STAT3 cascades and reduce ROS production.

scholarly journals Advanced Glycation End Products Are Associated With Physical Activity and Physical Functioning in the Older Population

2018 ◽  
Vol 73 (11) ◽  
pp. 1545-1551 ◽  
Author(s):  
Hans Drenth ◽  
Sytse U Zuidema ◽  
Wim P Krijnen ◽  
Ivan Bautmans ◽  
Andries J Smit ◽  
...  
Keyword(s):  
Physical Activity ◽  
Advanced Glycation End Products ◽  
Physical Functioning ◽  
Advanced Glycation ◽  
Older Population ◽  
Glycation End Products ◽  
End Products

scholarly journals Chondroprotective effects of purple corn anthocyanins on advanced glycation end products induction through suppression of NF-κB and MAPK signaling

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hathaichanok Chuntakaruk ◽  
Prachya Kongtawelert ◽  
Peraphan Pothacharoen
Keyword(s):  
Advanced Glycation End Products ◽  
Articular Chondrocytes ◽  
Cartilage Degradation ◽  
Mapk Signaling ◽  
Human Articular Chondrocytes ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
Purple Corn ◽  
Anti Inflammatory

AbstractFormation of advanced glycation end products (AGEs), which are associated with diabetes mellitus, contributes to prominent features of osteoarthritis, i.e., inflammation-mediated destruction of articular cartilage. Among the phytochemicals which play a role in anti-inflammatory effects, anthocyanins have also been demonstrated to have anti-diabetic properties. Purple corn is a source of three major anthocyanins: cyanidin-3-O-glucoside, pelargonidin-3-O-glucoside and peonidin-3-O-glucoside. Purple corn anthocyanins have been demonstrated to be involved in the reduction of diabetes-associated inflammation, suggesting that they may have a beneficial effect on diabetes-mediated inflammation of cartilage. This investigation of the chondroprotective effects of purple corn extract on cartilage degradation found a reduction in glycosaminoglycans released from AGEs induced cartilage explants, corresponding with diminishing of uronic acid loss of the cartilage matrix. Investigation of the molecular mechanisms in human articular chondrocytes showed the anti-inflammatory effect of purple corn anthocyanins and the metabolite, protocatechuic acid (PCA) on AGEs induced human articular chondrocytes via inactivation of the NFκb and MAPK signaling pathways. This finding suggests that purple corn anthocyanins and PCA may help ameliorate AGEs mediated inflammation and diabetes-mediated cartilage degradation.

scholarly journals CHARACTERISATION, IN SILICO AND IN VITRO DETERMINATION OF ANTIDIABETIC AND ANTI INFLAMMATORY POTENTIAL OF ETHANOLIC EXTRACT OF SARGASSUM WIGHTII.

2017 ◽  
Vol 10 (4) ◽  
pp. 297 ◽  
Author(s):  
Hemalatha S ◽  
Fazeela Mahaboob Begum
Keyword(s):  
Ascorbic Acid ◽  
Molecular Docking ◽  
Advanced Glycation End Products ◽  
Ethanolic Extract ◽  
Advanced Glycation ◽  
Sargassum Wightii ◽  
Glycation End Products ◽  
End Products ◽  
Anti Inflammatory

 Objective: The current study was intended to investigate and characterize the phytoconstituents of  Sargassum wightii ,evaluate its anti diabetic  and anti inflammatory potential under in silico and in vitro conditions.Methods: The marine algae S.wightii was extracted with ethanol. The ethanolic extract was screened for various phytoconstituents, quantified for total flavonoid and polyphenol content. FTIR and Mass spectrometry was used for characterizing the ethanolic extract. Antidiabetic potential of the phytoconstituents was analysed by molecular docking and enzyme inhibition assays. The anti inflammatory potential was evaluated by albumin denaturation assay.Results: The phytochemical screening revealed the presence of flavonoids, polyphenols, alkaloids, tannins, carbohydrates, proteins, oils and fat present in the ethanol extract. The FTIR analysis showed the presence of α,b unsaturated ketone, alcohol and ether groups in the extract.  MS analysis identified l - (+) - Ascorbic acid 2, 6 dihexadeconoate and Dotriacontyl isopropyl ether in the ethanolic extract. The molecular docking studies revealed that l - (+) - Ascorbic acid 2, 6 dihexadeconoate interacted with both α amylase and α glucosidase with a consensus score of 5.The results of  in vitro analysis showed that the ethanolic extract exhibited strong inhibitory activity on α amylase (IC 50 8mg/ml) and α glucosidase (IC 50 6mg/ml) respectively. The ethanolic extract also inhibited the formation of advanced glycation end products (IC 50 10mg/ml).Further the ethanolic extract inhibited the denaturation of albumin (IC 50 2.5 mg/ml) there by revealing the anti inflammatory potential of S.wightii.Conclusion: Hence it can be concluded that S.wightii possess anti diabetic and anti inflammatory potential which may be due to the presence of l - (+) - Ascorbic acid 2, 6 dihexadeconoate and flavonoids, polyphenols, alkaloids, tannins, carbohydrates, proteins, oils and fat present in the extract.Key words: Sargassum wightii, α amylase, α glucosidase, advanced glycation end products, molecular docking.

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