scholarly journals DNA Microarray-Based Global Gene Expression Profiling in Human Amniotic Epithelial Cells Predicts the Potential of Microalgae-Derived Squalene for the Nervous System and Metabolic Health

Biomedicines ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 48
Author(s):  
Farhana Ferdousi ◽  
Kinji Furuya ◽  
Kazunori Sasaki ◽  
Yun-Wen Zheng ◽  
Tatsuya Oda ◽  
...  
Keyword(s):  
Nervous System ◽  
Epithelial Cells ◽  
Dna Microarray ◽  
Enrichment Analysis ◽  
Nervous System Development ◽  
Gene Set Enrichment Analysis ◽  
Potential Health ◽  
Tissue Specific ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

In recent years, perinatal stem cells, such as human amniotic epithelial cells (hAECs), have attracted increasing interest as a novel tool of stem cell-based high-throughput drug screening. In the present study, we investigated the bioactivities of squalene (SQ) derived from ethanol extract (99.5%) of a microalgae Aurantiochytrium Sp. (EEA-SQ) in hAECs using whole-genome DNA microarray analysis. Tissue enrichment analysis showed that the brain was the most significantly enriched tissue by the differentially expressed genes (DEGs) between EEA-SQ-treated and control hAECs. Further gene set enrichment analysis and tissue-specific functional analysis revealed biological functions related to nervous system development, neurogenesis, and neurotransmitter modulation. Several adipose tissue-specific genes and functions were also enriched. Gene-disease association analysis showed nervous system-, metabolic-, and immune-related diseases were enriched. Altogether, our study suggests the potential health benefits of microalgae-derived SQ and we would further encourage investigation in EEA-SQ and its derivatives as potential therapeutics for nervous system- and metabolism-related diseases.

scholarly journals Therapeutic Potential of Human Amniotic Epithelial Cells on Injuries and Disorders in the Central Nervous System

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Huiming Xu ◽  
Jiaofei Zhang ◽  
Kam Sze Tsang ◽  
Hao Yang ◽  
Wei-Qiang Gao
Keyword(s):  
Stem Cells ◽  
Central Nervous System ◽  
Nervous System ◽  
Epithelial Cells ◽  
Embryonic Stem ◽  
Cell Based Therapy ◽  
Amniotic Epithelial Cells ◽  
Recent Advances ◽  
The Central Nervous System ◽  
Human Amniotic Epithelial Cells

Despite recent advances in neurosurgery and pharmaceuticals, contemporary treatments are ineffective in restoring lost neurological functions in patients with injuries and disorders of the central nervous system (CNS). Therefore, novel and effective therapies are urgently needed. Recent studies have indicated that stem cells, including embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and mesenchymal stem cells (MSCs), could repair/replace damaged or degenerative neurons and improve functional recovery in both preclinical and clinical trials. However, there are many unanswered questions and unsolved issues regarding stem cell therapy in terms of potency, stability, oncogenicity, immune response, cell sources, and ethics. Currently, human amniotic epithelial cells (hAECs) derived from the amnion exhibit considerable advantages over other stem cells and have drawn much attention from researchers. hAECs are readily available, pose no ethical concerns, and have little risk of tumorigenicity and immunogenicity. Mounting evidence has shown that hAECs can promote neural cell survival and regeneration, repair affected neurons, and reestablish damaged neural connections. It is suggested that hAECs may be the most promising candidate for cell-based therapy of neurological diseases. In this review, we mainly focus on recent advances and potential applications of hAECs for treating various CNS injuries and neurodegenerative disorders. We also discuss current hurdles and challenges regarding hAEC therapies.

scholarly journals Human amniotic epithelial cells are promising transgene carriers for allogeneic cell transplantation into liver

2000 ◽  
Vol 45 (3) ◽  
pp. 171-176 ◽  
Author(s):  
N. Sakuragawa ◽  
S. Enosawa ◽  
T. Ishii ◽  
R. Thangavel ◽  
T. Tashiro ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Transplantation ◽  
Allogeneic Cell ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

Expression of markers for both neuronal and glial cells in human amniotic epithelial cells

1996 ◽  
Vol 209 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Norio Sakuragawa ◽  
Ramasamy Thangavel ◽  
Masashi Mizuguchi ◽  
Motoyuki Hirasawa ◽  
Isao Kamo
Keyword(s):  
Epithelial Cells ◽  
Glial Cells ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

scholarly journals Human Amniotic Epithelial Cells Possess Hepatocyte-like Characteristics and Functions

2004 ◽  
Vol 29 (3) ◽  
pp. 73-84 ◽  
Author(s):  
Seiji Takashima ◽  
Hirohiko Ise ◽  
Peng Zhao ◽  
Toshihiro Akaike ◽  
Toshio Nikaido
Keyword(s):  
Epithelial Cells ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

scholarly journals Functional Screening of TLRs in Human Amniotic Epithelial Cells

2011 ◽  
Vol 187 (5) ◽  
pp. 2766-2774 ◽  
Author(s):  
Claire Gillaux ◽  
Céline Méhats ◽  
Daniel Vaiman ◽  
Dominique Cabrol ◽  
Michelle Breuiller-Fouché
Keyword(s):  
Epithelial Cells ◽  
Functional Screening ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

scholarly journals Establishment and Characterization of Immortalized Human Amniotic Epithelial Cells

2013 ◽  
Vol 15 (1) ◽  
pp. 55-67 ◽  
Author(s):  
Kaixuan Zhou ◽  
Chika Koike ◽  
Toshiko Yoshida ◽  
Motonori Okabe ◽  
Moustafa Fathy ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

scholarly journals Pathway Interactions Based on Drug-Induced Datasets

2019 ◽  
Vol 18 ◽  
pp. 117693511985151 ◽  
Author(s):  
Shinuk Kim
Keyword(s):  
Breast Cancer ◽  
Valproic Acid ◽  
Epithelial Cells ◽  
Sample Size ◽  
Drug Combination ◽  
Trichostatin A ◽  
Enrichment Analysis ◽  
Gene Set Enrichment Analysis ◽  
Gene Set Enrichment ◽  
Drug Induced

In this study, we identified enrichment pathway connections from MCF7 breast cancer epithelial cells that were treated with 87 drugs. We extracted drug-treated samples, where the sample size was greater than or equal to 5. The drugs included 17-allylamino-geldanamycin, LY294002, trichostatin A, valproic acid, sirolimus, and wortmannin, which had sample sizes of 11, 8, 7, 7, 7, and 5, respectively. We found meaningful pathways using gene set enrichment analysis and identified intradrug and interdrug pathway interactions, which implied the influence of drug combination. Among the top 20 enrichment pathways that were wortmannin induced, there were a total of 37 intradrug pathway interactions via common genes. Thirty-seven pathway interactions were induced by valproic acid, 11 induced by trichostatin A, 20 induced by LY294002, and 59 induced by sirolimus, all via common genes. The number of interdrug-induced pathway interactions ranged from one pair of pathways to 23. The pair of ERBB_SIGNALING and INSULIN_SIGNALING pathways showed the highest score from a pair of 2 individual drugs. The highest number of pathway interactions was observed between the drugs 17-allylamino-geldanamycin and LY294002.

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