scholarly journals Salivary miR-30c-5p as Potential Biomarker for Detection of Oral Squamous Cell Carcinoma

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1079
Author(s):  
Nikolay Mehterov ◽  
Boyan Vladimirov ◽  
Andrea Sacconi ◽  
Claudio Pulito ◽  
Marcin Rucinski ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Target Identification ◽  
Taqman Assay ◽  
Invasive Approach ◽  
Non Invasive ◽  
Bodily Fluids ◽  
Potential Biomarker

The levels of different classes of extracellular RNAs (exRNAs) remain stable in bodily fluids. The detection of either enriched or depleted specific subsets of salivary microRNAs (miRNAs) has the potential to serve as a non-invasive approach for biomarker development. Thus, salivary miRNAs have emerged as a promising molecular tool for early diagnosis and screening of oral squamous cell carcinoma (OSCC). Total RNA was extracted from saliva supernatant of 33 OSCC patients and 12 controls (discovery set), and the differential expression of 8 cancer-related miRNAs was detected by TaqMan assay. Among the screened miRNAs, miR-30c-5p (p < 0.04) was significantly decreased in OSCC saliva. The same transcriptional behavior of miR30c-5p was observed in an additional validation set. miR-30c-5p showed a significant statistical difference between cases and controls with areas under the curve (AUC) of 0.82 (95% CI: 0.71–0.89). The sensitivity and the specificity of miR-30c-5p were 86% and 74%, respectively. The target identification analysis revealed enrichment of miR-30c-5p targets in p53 and Wnt signaling pathways in OSCC. Additionally, the miR-30c-5p targets had clinical significance related to overall survival. In conclusion, these findings show that downregulated miR-30c-5p has the potential to serve as a novel, non-invasive biomarker for early OSCC detection.

Role of pretreatment neutrophil-to-lymphocyte ratio as an independent prognostic factor in oral squamous cell carcinoma patients: A prospective study in a tertiary care center

2018 ◽  
Vol 3 (2) ◽  
pp. 37
Author(s):  
Shashwat Tiwari ◽  
Shivani Nautiyal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tertiary Care ◽  
Prognostic Index ◽  
Prognostic Scores ◽  
Lymphocyte Ratio ◽  
Potential Biomarker ◽  
The Absolute

<p><strong>Background:</strong> More recently, established systemic inflammation-based prognostic scores have been explored extensively, such as neutrophil-lymphocyte ratio (NLR) and serum C-reactive protein. We postulated that NLR might be a readily available and inexpensive objective prognostic index that could be used in daily oncologic clinical practice and could help to stratify patients in clinical trials.<br /><strong>Materials and Methods:</strong> In total, there were 150 patients with oral squamous cell carcinoma (OSCC) treated at GSVM Medical College, Kanpur, between October 2012 and January 2015 whose clinical information and laboratory parameters were obtained. The NLR was determined by dividing the absolute neutrophil count by the absolute lymphocyte count, and the NLR data were then dichotomized and divided into two groups as NLR low and high. <strong>Conclusion:</strong> Our findings reported herein demonstrated that pretreatment NLR is a potential biomarker for predicting the overall survival in OSCC patients. Combined with other markers, NLR may be used in decision-making and the selection of treatment modality in patients with oral SCC.</p>

scholarly journals Overexpression of Stathmin in Oral Squamous Cell Carcinoma, its Association with Histomorphological Features and Pathological Staging

2021 ◽  
Author(s):  
Nabanita Barma ◽  
Gopinath Barui ◽  
Anadi Roy Chowdhury
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cancer Progression ◽  
Squamous Cell ◽  
Tertiary Care ◽  
Clinicopathological Parameters ◽  
Pathological Staging ◽  
Potential Biomarker ◽  
Tobacco Chewing

Introduction: Stathmin, one of the most important intracellular phosphoprotein that plays the vital role in the regulation of cell cycle and cell proliferation. It acts by disassembly of Microtubules (MT) that helps in the formation of mitotic spindle. It is overexpressed in various human cancers. Aim: To investigate the expression status of stathmin and its relation with clinicopathologic significance in Oral Squamous Cell Carcinoma (OSCC) and its association with age, sex, tobacco chewing, histomorphological features and pathological staging. Materials and Methods: A descriptive and observational study in cross-sectional design was conducted in a tertiary care centre of Kolkata, West Bengal, India, from January 2020 to March 2020 in the Department of Pathology of RG Kar Medical College and Hospital in collaboration with Department of Oto-Rhino-Laryngology of the same institute. Stathmin expression was assessed by immunohistochemistry (IHC) in 28 OSCC cases. The association between stathmin expression and clinicopathological parameters like age, sex, tobacco chewing, tumour site, histomorphological type, Tumor Nodes Metastases (TNM) stage and Worst Pattern Of Invasion (WPOI) were evaluated on the basis of Chi-square test and Fisher’s-exact test (software Statistical Package for the Social Sciences (SPSS) V 25.0). Results: Stathmin was overexpressed in 18 cases out of 28 cases of OSCC. In this study a positive association was observed between stathmin expression and age group, history of tobacco chewing, advanced T stage, advanced pTNM staging and WPOI of OSCC. Conclusion: The study suggests that overexpression of stathmin could contribute to cancer progression. There is a higher likelihood that stathmin may be used as a potential biomarker as well as therapeutic target for OSCC.

Cripto 1, a potential biomarker for oral squamous cell carcinoma

Oral Oncology ◽  
2019 ◽  
Vol 90 ◽  
pp. 153-154 ◽  
Author(s):  
Anu Jain ◽  
Sumanth Mallupattu ◽  
Reetu Thakur ◽  
Sushmita Ghoshal ◽  
Arnab Pal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Potential Biomarker

scholarly journals HISTOPATHOLOGICAL GRADING OF ORAL SQUAMOUS CELL CARCINOMA BY MICRONUCLEI ASSAY, A POTENTIAL BIOMARKER IN ORAL EXFOLIATED CELLS

2021 ◽  
pp. 95-99
Author(s):  
Noratan Jatiya ◽  
Vijay Rajak ◽  
Savita Soni
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tobacco Consumption ◽  
Control Group ◽  
Exfoliated Cells ◽  
Potential Biomarker ◽  
The Difference ◽  
Histopathological Grading

Background: Micronucleus is a microscopically visible round or oval cytoplasmic chromatin mass in the extra nuclear vicinity, originated from aberrant mitosis, which consists of eccentric chromosomes that have failed to reach spindle poles during mitosis and are used as biomarkers for assessment of DNA damage. Micronuclei in exfoliated oral epithelial cells have been shown in some studies to correlate with severity of this genotoxic damage. This severity can be measured in terms of grading of the lesions. Aim: To correlate frequency of micronuclei (MN) in oral exfoliated cells in clinically diagnosed cases of oral squamous cell carcinoma (OSCC) followed by a histopathological grading. Materials and Methods : The study subjects consisted of clinically diagnosed cases of OSCC. Healthy subjects without any tobacco consumption habits formed the control group.The cytosmears from both groups were stained with rapid Papanicolaou stain. MN were identified according to the criteria given by Tolbert et al. Results: The frequency of MN was three to four times higher in patients with OSCC as compared to patients in the control group and the difference was found to be highly significant. In 75% cases, the cytological grade as determined by the frequency of micronuclei correlated with the histopathological grade and this observation was statistically significant. Conclusions : The MN is potentially an excellent candidate to serve as such a biomarker for prediction of the grade of OSCC.

scholarly journals Spectrophotometry of Saliva of Oral Squamous CellCarcinoma Patients

2009 ◽  
Vol 6 (2) ◽  
pp. 373-378
Author(s):  
Baghdad Science Journal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cancer ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Ir Spectrum ◽  
Human Saliva ◽  
Test Tube ◽  
Non Invasive ◽  
Back Ground

Back ground: Spectral analyses of solutions have long been applied to various body fluids for the purpose of clinical study as well as research .Human saliva can be easily obtained by non invasive .In this study typical spectra (for UV and IR) of saliva of oral cancer Squamous cell carcinoma patients were determined under average conditions and evaluated in relation to the spectra of normal specimens. Materials and Methods: Seventeen patients of oral cancer Squamous cell carcinoma and seventeen age matched healthy subject were included in this study .Chewing - Stimulated Saliva was collected in plastic test tube and stored at -20° C. Bach of saliva samples were used for UV and IR measurements. Results: Many differences between the IR spectrum of saliva of oral Squamous cell carcinoma patients and IR spectrum of normal saliva. Conclusion: The results in this study were detected that the use of IR spectroscopy may be useful in the diagnosis of oral Squamous cell carcinoma by using saliva samples.

scholarly journals Non-invasive early diagnosis of oral squamous cell carcinoma using piezoelectric biosensor

2020 ◽  
Vol 5 (3) ◽  
pp. 146
Author(s):  
Irene E. Rieuwpassa ◽  
Sumintarti Sumintarti ◽  
Yu-Ri Kim ◽  
Megatriani Matandung ◽  
Gabriel SA. Matongan
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Early Diagnosis ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Non Invasive
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