scholarly journals CD147 Promotes Cell Small Extracellular Vesicles Release during Colon Cancer Stem Cells Differentiation and Triggers Cellular Changes in Recipient Cells

Cancers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 260 ◽  
Author(s):  
Donatella Lucchetti ◽  
Filomena Colella ◽  
Luigi Perelli ◽  
Claudio Ricciardi-Tenore ◽  
Federica Calapà ◽  
...  
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Multivesicular Body ◽  
Cellular Changes ◽  
Colon Cancer Stem Cells ◽  
Colorectal Cancer Stem Cells

Cancer cells secrete small extracellular vesicles (sEVs) that are involved in the remodeling of tumor microenvironment (TME) and can promote tumor progression. The role of sEVs and their molecular key players in colon cancer stem cells differentiation are poorly understood. This study aimed to analyze the role and content of sEVs released during the differentiation of colorectal cancer stem cells. Here we show that sEVs secretion during colon cancer stem cells differentiation is partially controlled by CD147, a well-known player involved in colon cancer tumorigenesis. CD147 + sEVs activate a signaling cascade in recipient cells inducing molecular invasive features in colon cancer cells. CD147 knockdown as well as anti-CD147 antibodies impaired sEVs release and downstream effects on recipient cells and blocking multivesicular body maturation prevented sEVs release during the differentiation. Our findings reveal a functional role of CD147 in promoting sEVs release during the differentiation of colon cancer stem cells and in triggering cellular changes in recipient cells.

Sa1156 ROLE OF WNT NON-CANONICAL PATHWAY IN MAINTENANCE OF COLON CANCER STEM CELLS.

2020 ◽  
Vol 158 (6) ◽  
pp. S-293
Author(s):  
Aditi Bhattacharya ◽  
Devanjan Dey ◽  
Janvie Manhas ◽  
Muzaffar Bhat ◽  
Jayanth Kumar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Cancer Stem Cells ◽  
Canonical Pathway ◽  
Colon Cancer Stem Cells

scholarly journals Curcumin Suppresses Crosstalk between Colon Cancer Stem Cells and Stromal Fibroblasts in the Tumor Microenvironment: Potential Role of EMT

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e107514 ◽  
Author(s):  
Constanze Buhrmann ◽  
Patricia Kraehe ◽  
Cora Lueders ◽  
Parviz Shayan ◽  
Ajay Goel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Tumor Microenvironment ◽  
Cancer Stem Cells ◽  
Potential Role ◽  
Stromal Fibroblasts ◽  
Colon Cancer Stem Cells

scholarly journals An integrated microfluidic system for screening of phage-displayed peptides specific to colon cancer cells and colon cancer stem cells

2015 ◽  
Vol 9 (5) ◽  
pp. 054121 ◽  
Author(s):  
Yu-Jui Che ◽  
Huei-Wen Wu ◽  
Lien-Yu Hung ◽  
Ching-Ann Liu ◽  
Hwan-You Chang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Microfluidic System ◽  
Colon Cancer Cells ◽  
Colon Cancer Stem Cells

scholarly journals Crucial Role of Interleukin-4 in the Survival of Colon Cancer Stem Cells: Figure 1.

2008 ◽  
Vol 68 (11) ◽  
pp. 4022-4025 ◽  
Author(s):  
Maria Giovanna Francipane ◽  
Mileidys Perez Alea ◽  
Ylenia Lombardo ◽  
Matilde Todaro ◽  
J.P. Medema ◽  
...  
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Cancer Stem Cells ◽  
Crucial Role ◽  
Interleukin 4 ◽  
Colon Cancer Stem Cells

scholarly journals Registered report: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
James Evans ◽  
Anthony Essex ◽  
Hong Xin ◽  
Nurith Amitai ◽  
Lindsey Brinton ◽  
...  
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Cell Biology ◽  
Cancer Biology ◽  
Stem Cell Markers ◽  
Colon Cancer Cells ◽  
Colon Cancer Stem Cells

The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by replicating selected results from a substantial number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (<xref ref-type="bibr" rid="bib5">Errington et al., 2014</xref>). This Registered report describes the proposed replication plan of key experiments from ‘Wnt activity defines colon cancer stem cells and is regulated by the microenvironment’ by Vermeulen and colleagues, published in Nature Cell Biology in 2010 (<xref ref-type="bibr" rid="bib20">Vermeulen et al., 2010</xref>). The key experiments that will be replicated are those reported in Figures 2F, 6D, and 7E. In these experiments, Vermeulen and colleagues utilize a reporter for Wnt activity and show that colon cancer cells with high levels of Wnt activity also express cancer stem cell markers (Figure 2F; <xref ref-type="bibr" rid="bib20">Vermeulen et al., 2010</xref>). Additionally, treatment either with conditioned medium derived from myofibroblasts or with hepatocyte growth factor restored clonogenic potential in low Wnt activity colon cancer cells in vitro (Figure 6D; <xref ref-type="bibr" rid="bib20">Vermeulen et al., 2010</xref>) and in vivo (Figure 7E; <xref ref-type="bibr" rid="bib20">Vermeulen et al., 2010</xref>). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange and the results of the replications will be published in eLife.

scholarly journals The critical role of peroxiredoxin-2 in colon cancer stem cells

Aging ◽  
2021 ◽  
Author(s):  
Linglong Peng ◽  
Yongfu Xiong ◽  
Rong Wang ◽  
Ling Xiang ◽  
He Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Cancer Stem Cells ◽  
Critical Role ◽  
Peroxiredoxin 2 ◽  
Colon Cancer Stem Cells

scholarly journals E2F7 Transcriptionally Inhibits MicroRNA-199b Expression to Promote USP47, Thereby Enhancing Colon Cancer Tumor Stem Cell Activity and Promoting the Occurrence of Colon Cancer

2021 ◽  
Vol 10 ◽  
Author(s):  
Xiong Guo ◽  
Ling Liu ◽  
Qi Zhang ◽  
Weiming Yang ◽  
Yang Zhang
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Cell Activity ◽  
Luciferase Reporter ◽  
Tumor Stem Cell ◽  
Colon Cancer Cells ◽  
Loss Of Function ◽  
Colon Cancer Stem Cells

microRNAs (miRNAs) can modulate the expression level of genes in a post-transcription manner, which are closely related to growth and metastasis of colon cancer. Herein, we aimed to explore how miR-199b influences colon cancer and to characterize its underlying molecular mechanism associating with E2F transcription factor 7 (E2F7). Assays of RT-qPCR, Western blot, and immunohistochemistry were utilized to detect the expression of E2F7 in the tissue samples collected from 30 patients diagnosed with colon cancer. Flow analysis was utilized to detect the ratio of ALDH1+ and CD133+ colon cancer stem cells. The interaction between E2F7, miR-199b, USP47, and MAPK was identified by ChIP-Seq analysis, luciferase reporter, RNA pull-down, co-immunoprecipitation, as well as glutathione-S-transferase (GST) pull-down experiments. Based on the gain- and loss-of-function approaches, the cellular functions of colon cancer cells by the E2F7-regulated miR-199b/USP47/MAPK axis were assessed. It was identified that E2F7 are expressed highly in the collected colon cancer tissues. E2F7 silencing reduced the production of ALDH1+ and CD133+ colon cancer stem cells and antagonized the effects of 5-fluorouracil (5-FU) treatment. Besides, the silencing of E2F7 was observed to suppress the oxidative stress, proliferation, migration, as well as invasion of ALDH1+ cells in vitro and tumorigenesis of colon cancer cells in vivo. Our findings reveal the pro-oncogenic effect of E2F7 on colon cancer development, highlighting E2F7 as a novel target for therapeutic strategy for colon cancer.

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