Isolation and Identification of an Efficient Aerobic Denitrifying Pseudomonas stutzeri Strain and Characterization of Its Nitrite Degradation

Nitrogen pollution in water bodies is becoming increasingly serious, and how to remove nitrogen from water bodies economically and effectively has become a research hotspot. Especially in recent years, with the gradual expansion of aquaculture in China, the content of nitrite and other nitrogen-containing substances in water bodies has been increasing, which inhibits the growth of farm animals and is one of the causes of eutrophication in water bodies. In this study, a strain of bacteria was isolated from the sludge of an aquaculture fishpond and identified as Pseudomonas stutzeri, which can efficiently degrade nitrite. After continuous domestication in nitrite mixed solution, the nitrite nitrogen reduction capacity of P. stutzeri was significantly improved. Univariate experiments aiming to optimize the degradation conditions indicate that the optimal culture conditions for strain F2 are: medium with a carbon source of sodium succinate; C/N of 18; pH of 8; culture temperature of 28 °C; and shaking speed of 210 rpm in the shaker. Under the optimal culture conditions, the NO2−-N concentration of the culture solution was 300 mg/L, and the nitrite removal rate reached 98.67%. Meanwhile, the results of the nitrogen balance test showed that the strain converted 6.1% of the initial nitrogen into cellular organic nitrogen and 62.3% into gaseous nitrogen.

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Ammonium removal by a novel heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas stutzeri KTB from wastewater

Water Quality Research Journal ◽

10.2166/wqrjc.2015.031 ◽

2015 ◽

Vol 50 (3) ◽

pp. 219-227 ◽

Cited By ~ 1

Author(s):

Maohong Zhou ◽

Hairen Ye ◽

Xiaowei Zhao

Keyword(s):

Nitrogen Removal ◽

Removal Rate ◽

Pseudomonas Stutzeri ◽

Culture Conditions ◽

Aerobic Denitrification ◽

Ammonium Concentration ◽

Logarithmic Phase ◽

Ammonium Removal ◽

Removal Ratio ◽

Initial Ph

The effects of culture conditions on a newly isolated Pseudomonas stutzeri KTB's ability to simultaneously perform heterotrophic nitrification and aerobic denitrification were investigated to determine its potential of application in nitrogen removal from wastewater. The results from experiments in the presence of 10 mmol/L of ammonium were as follows: succinate was the preferred carbon source, and the optimum C/N ratio, temperature, and initial pH were 10, 30 °C, and 7–8, respectively. Nitrogen removal took place not only in the logarithmic phase but also in the stationary phase. Under the optimum conditions, the nitrogen removal rate increased as the ammonium concentration elevated, until it was as high as 60 mmol/L. Meanwhile, the maximum specific growth rate decreased. The highest nitrogen removal rate of 0.977 mmol/L/h was observed at 60 mmol/L of ammonium and the maximum removal ratio of 85.6% at 40 mmol/L when the bacterial treatment for 48 h was completed. The strain was vulnerable to even higher ammonium loads. When incubated in anaerobically digested hennery wastewater containing 43.85 mmol/L of ammonium and 2.32 mmol/L of nitrate, the removal ratio and rate reached 82.4% and 0.397 mmol/L/h, respectively. The strain might be a great candidate for ammonium removal from wastewater.

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Simultaneous heterotrophic nitrification and aerobic denitrification at high concentrations of NaCl and ammonia nitrogen by Halomonas bacteria

Water Science & Technology ◽

10.2166/wst.2017.214 ◽

2017 ◽

Vol 76 (2) ◽

pp. 386-395 ◽

Cited By ~ 9

Author(s):

Te Wang ◽

Jian Li ◽

Ling Hua Zhang ◽

Ying Yu ◽

Yi Min Zhu

Keyword(s):

Removal Rate ◽

Sodium Succinate ◽

Bacterial Genome ◽

Ammonia Nitrogen ◽

Compatible Solute ◽

Heterotrophic Nitrification ◽

Aerobic Denitrification ◽

Sequencing Analysis ◽

N Removal ◽

High Concentrations

To improve the efficiency of simultaneous heterotrophic nitrification and aerobic denitrification (SND) at high concentrations of NaCl and ammonia nitrogen (NH4+—N), we investigated the SND characteristics of Halomonas bacteria with the ability to synthesize the compatible solute ectoine. Halomonas sp. strain B01, which was isolated, screened and identified in this study, could simultaneously remove nitrogen (N) by SND and synthesize ectoine under high NaCl conditions. Gene cloning and sequencing analysis indicated that this bacterial genome contains ammonia monooxygenase (amoA) and nitrate reductase (narH) genes. Optimal conditions for N removal in a solution containing 600 mg/L NH4+–N were as follows: sodium succinate supplied as organic carbon (C) source at a C/N ratio of 5, pH 8 and shaking culture at 90 rpm. The N removal rate was 96.0% under these conditions. The SND by Halomonas sp. strain B01 was performed in N removal medium containing 60 g/L NaCl and 4,000 mg/L NH4+–N; after 180 h the residual total inorganic N concentration was 21.7 mg/L and the N removal rate was 99.2%. Halomonas sp. strain B01, with the ability to synthesize the compatible solute ectoine, could simultaneously tolerate high concentrations of NaCl and NH4+–N and efficiently perform N removal by SND.

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Study on Degradation of Traditional Chinese Medicine Wastewater with Cu/Cu2O Composite Photocatalyst

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.989-994.1073 ◽

2014 ◽

Vol 989-994 ◽

pp. 1073-1076

Author(s):

Chun Hua Yuan

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Removal Rate ◽

Average Particle Size ◽

Copper Nitrate ◽

Thermal Method ◽

Average Particle ◽

Mixed Solution ◽

Composite Photocatalyst ◽

Cod Removal Rate

s. This paper uses alcohol thermal method, takes the mixed solution of N,N-two DMF and ethanol as solvent, in particular concentration of copper nitrate, prepares composite photocatalyst Cu/Cu2O by control of temperature, then investigates the composite with XRD. The experimental results show that, the catalyst prepared has good dispersing, its average particle size is 7.96nm, and Cu comes into the Cu2O lattice conducted the corresponding lattice distortions. In the 40W tungsten lamp irradiation, the photocatalyst can achieve effective degradation of traditional Chinese medicine wastewater, whose degradation rate can reach 97.3% and the COD removal rate can reach 95.6%.

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Optimization of Cultivation Condition of Newly Isolated Strain Chlorella Sorokiniana pa.91 for CO2 Bio-Fixation and Nutrients Removal From Real Municipal Wastewater: Impact of Temperature and Light Intensity

10.21203/rs.3.rs-195210/v1 ◽

2021 ◽

Author(s):

Poone Yaqoubnejad ◽

Hassan Aminirad ◽

Mohsen Taghavijeloudar

Keyword(s):

Municipal Wastewater ◽

Removal Rate ◽

High Capacity ◽

Biomass Concentration ◽

Biomass Productivity ◽

Batch Cultivation ◽

Culture Conditions ◽

Chlorella Sorokiniana ◽

Synthetic Wastewater ◽

Cultivation Condition

Abstract The cultivation conditions of a newly isolated strain Chlorella sorokiniana pa.91 were optimized for the first time by performing sixty batch cultivation experiments at various temperatures (20, 25, 30 and 35 °C) and light intensities (1000, 3000, 4000, 5000 and 7000 Lux) in three different culture mediums of BG-11, real settled municipal wastewater (RMWW) and synthetic wastewater (SWW). Additionally, to evaluate the capability of C. sorokiniana pa.91 in CO2 bio-fixation and wastewater treatment, the microalgae was cultivated in a flat-plate photobioreactor (CO2 = 16% and 0.6 vvm aeration) under the optimal condition. The optimization results suggested that at the culture conditions of 30 °C, 4000 Lux and RMWW (COD 211 mgL-1) microalgae had the best performance in growth and biomass productivity. Maximum biomass concentration and productivity of 3.21 gL-1 and 0.31 gL-1d-1were achieved, respectively, by cultivation of C. sorokiniana pa.91 in the photobioreactor under the optimized condition. Experimental results showed that C. sorokiniana pa.91 has a high capacity of CO2 bio-fixation (0.59 mgL-1d-1) and CO2 removal rate (35.6 %). Moreover, using C. sorokiniana pa.91 could efficiently remove 74% of NH3, 93% of NO3-, 83% of PO4-3 and 76% of COD from real municipal wastewater after eight days of cultivation in the photobioreactor.

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Isolation and Characterization of Pseudomonas stutzeri as Lead Tolerant Bacteria from Water Bodies of Udaipur, India using 16S rDNA Sequencing Technique

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.11.2.39 ◽

2017 ◽

Vol 11 (2) ◽

pp. 975-979 ◽

Cited By ~ 2

Author(s):

Garima Verma ◽

Nadim Chishty ◽

Chandra Veer

Keyword(s):

16S Rdna ◽

Pseudomonas Stutzeri ◽

Water Bodies ◽

16S Rdna Sequencing ◽

Isolation And Characterization ◽

Rdna Sequencing ◽

Sequencing Technique

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Eksplorasi Bakteri Indigen Pada Pembenihan Ikan Lele Dumbo (Clarias sp.) Sistem Resirkulasi Tertutup [Exploration Of Indigen Bacteria From Catfish (Clarias sp.) Breeding On Closed Resirculation System ]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v4i2.11571 ◽

2019 ◽

Vol 4 (2) ◽

pp. 193

Author(s):

Prayogo, Boedi Setya Rahardja, Abdul Manan

Keyword(s):

Organic Matter ◽

Organic Materials ◽

Pseudomonas Stutzeri ◽

Bacterial Strains ◽

Isolation And Identification ◽

Biological Filtration ◽

Degrading Bacteria ◽

Recirculation System ◽

Pseudomonas Pseudomallei

Abstract The efforts of the catfish hatchery fish are generally confined to the central areas of hatchery that has abundant water resources. Solving the problem of limited water, appears a catfish hatchery system with a closed recirculation system. In such systems the process of biological filtration is the most important thing. It shows the handling of organic materials by utilizing the degrading bacteria is the key to successful management of the system. Thus necessary to be studied the role of bacteria degrading organic matter indigen (local bacteria) in the closed hatchery recirculation system of catfish. This study aimed to obtain bacterial strains degrading bacteria indigen as organic materials are very useful in improving water quality in the closed hatchery recirculation system of catfish and knowing the methods to the use of bacteria indigen as degrading organic matter. The method used in this study was designed based on the stages of research carried out in accordance with the objectives to be achieved. The results of the isolation and identification to the species level showed Pseudomonas stutzeri and Pseudomonas pseudomallei obtain the greatest value for hydrolysis index representing each trait protease, lipase and amylase. Bioremediation effectiveness test in vitro showed effective results in the treatment using consortia of bacteria inoculant. Consortia of bacteria inoculant effective in improving the growth rate and survival rate (SR) in the closed hatchery recirculation system of catfish.

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Biodegradation of Major Pollutants in Yeast Wastewater by Using Immobilized Micro-Aerobic Microorganism Technology

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.675-677.493 ◽

2014 ◽

Vol 675-677 ◽

pp. 493-500

Author(s):

Jie Fu ◽

Xiao Fei Wang ◽

Chao Bing Deng ◽

Ping Xian

Keyword(s):

Removal Rate ◽

Oxygen Demand ◽

Pseudomonas Stutzeri ◽

Ammonia Nitrogen ◽

Economic Method ◽

Infrared Spectrometer ◽

Dominant Bacteria ◽

Degradation Effect ◽

Yeast Wastewater ◽

Composite Carrier

In order to provide an effective and economic method for the yeast wastewater′s treatment, this study taken yeast wastewater as objection, and applied immobilized micro-aerobic microorganism technology into yeast wastewater′s treatment, then explored the degradation effect of major pollutants in yeast wastewater. The immobilized carrier was analyzed by scanning electron microscope (SEM) and Fourier transform infrared spectrometer (FTIR), and dominant species in immobilized carrier were identified. The results showed that immobilized carrier was a composite carrier with porous structure, and removal rate of chemical oxygen demand (COD), ammonia nitrogen (NH3-N), total nitrogen (TN) and total organic carbon (TOC) of yeast wastewater by using the immobilized micro-aerobic microorganism technology were 71.1%, 99.7%, 61.4% and 67.0%, respectively. Moreover, the dominant bacteria which attached in the carrier included Sphingomonaspaucimobilis, Pseudomonas putida, Acinetobacterlwoffii and Pseudomonas stutzeri.

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Contribution of fish farmers to conservation of endangered Lake Victoria Basin fish species - the case of Oreochromis variabilis and O. esculentus.

10.1079/ac.109984.20203482976 ◽

2020 ◽

Author(s):

Harrison Charo-Karisa ◽

Jacob Maithya

Keyword(s):

Growth Performance ◽

Fish Species ◽

Lake Victoria ◽

Culture Conditions ◽

Water Bodies ◽

Lake Victoria Basin ◽

Endangered Fish ◽

Small Water ◽

Course Of Action

Abstract This paper discussed the conservation efforts of fish farmers of two endangered fish species in Lake Victoria namely Oreochromis variabilis and O. esculentus. Highlights focused on the determination of their growth performance under culture conditions, assessing their suitability for aquaculture, recruiting farmers to culture the species and testing the suitability of new dams and ponds for aquaculture. Both species breed easily under culture conditions. Therefore production of the fingerlings and their subsequent stocking in ponds, small water bodies and other larger water masses, including Lake Victoria, was a course of action implemented by the fish farmers to bring about their restoration.

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