scholarly journals Cutaneotrichosporon oleaginosus: A Versatile Whole-Cell Biocatalyst for the Production of Single-Cell Oil from Agro-Industrial Wastes

Catalysts ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1291
Author(s):  
Nicola Di Fidio ◽  
Filippo Minonne ◽  
Claudia Antonetti ◽  
Anna Maria Raspolli Galletti
Keyword(s):  
Single Cell ◽  
Oleaginous Yeast ◽  
State Of The Art ◽  
Lipid Production ◽  
Industrial Applications ◽  
Industrial Wastes ◽  
Single Cell Oil ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
By Products

Cutaneotrichosporon oleaginosus is an oleaginous yeast with several favourable qualities: It is fast growing, accumulates high amounts of lipids and has a very broad substrate spectrum. Its resistance to hydrolysis by-products makes it a promising biocatalyst for custom tailored microbial oils. C. oleaginosus can accumulate up to 60 wt.% of its biomass as lipids. This species is able to grow by using several compounds as a substrate, such as acetic acid, biodiesel-derived glycerol, N-acetylglucosamine, lignocellulosic hydrolysates, wastepaper and other agro-industrial wastes. This review is focused on state-of-the-art innovative and sustainable biorefinery schemes involving this promising yeast and second- and third-generation biomasses. Moreover, this review offers a comprehensive and updated summary of process strategies, biomass pretreatments and fermentation conditions for enhancing lipid production by C. oleaginosus as a whole-cell biocatalyst. Finally, an overview of the main industrial applications of single-cell oil is reported together with future perspectives.

scholarly journals Single Cell Oil Production from Waste Biomass: Review of Applicable Industrial By-Products

2019 ◽  
Vol 23 (2) ◽  
pp. 325-337
Author(s):  
Kriss Spalvins ◽  
Ilze Vamza ◽  
Dagnija Blumberga
Keyword(s):  
Single Cell ◽  
Industrial Waste ◽  
Essential Fatty Acids ◽  
Production Costs ◽  
Industrial Wastes ◽  
Biodiesel Production ◽  
Waste Materials ◽  
Single Cell Oil ◽  
Waste Products ◽  
By Products

Abstract Single cell oil (SCO) is an attractive alternative source of oil, which, depending on the fatty acid composition, can be used as a feedstock for biodiesel production, as an ingredient for pharmaceuticals or as a source of essential fatty acids for human and animal consumption. However, the use of SCO is limited due to use of relatively expensive food or feed products in the cultivation of SCO producing microorganisms. In order to reduce SCO production costs, the use of cheaper feedstock such as biodegradable agro-industrial wastes are necessary. At the same time, the microbial treatment of biodegradable wastes ensures the neutralization of environmentally harmful compounds and reduces the negative impact on the environment. Oleaginous microorganisms are capable of fermenting a variety of industrial by-products, waste products and wastewaters, however further discussion on properties of the waste materials is necessary to facilitate the selection of the most appropriate waste materials for SCO production. Thus, this review compares various industrial waste products that can be used as cheap feedstock for the cultivation of SCO producing microorganisms. Industrial waste products, by-products and wastewaters are compared according to their global availability, current use in competing industries, required pre-fermentation treatments, oleaginous microorganism cell concentrations and SCO yields.

Overproduction of single cell oil from xylose rich sugarcane bagasse hydrolysate by an engineered oleaginous yeast Rhodotorula mucilaginosa IIPL32

Fuel ◽  
2019 ◽  
Vol 254 ◽  
pp. 115653 ◽  
Author(s):  
Sheetal Bandhu ◽  
Neha Bansal ◽  
Diptarka Dasgupta ◽  
Vivek Junghare ◽  
Arushdeep Sidana ◽  
...  
Keyword(s):  
Single Cell ◽  
Sugarcane Bagasse ◽  
Oleaginous Yeast ◽  
Rhodotorula Mucilaginosa ◽  
Single Cell Oil ◽  
Sugarcane Bagasse Hydrolysate

Single Cell Oil from Oleaginous Yeast Grown on Sugarcane Bagasse-Derived Xylose: An Approach toward Novel Biolubricant for Low Friction and Wear

2017 ◽  
Vol 6 (1) ◽  
pp. 275-283 ◽  
Author(s):  
Sheetal Bandhu ◽  
Mahesh B. Khot ◽  
Tripti Sharma ◽  
Om P. Sharma ◽  
Diptarka Dasgupta ◽  
...  
Keyword(s):  
Single Cell ◽  
Sugarcane Bagasse ◽  
Friction And Wear ◽  
Oleaginous Yeast ◽  
Single Cell Oil ◽  
Low Friction

scholarly journals Ranking of By-products for Single Cell Oil Production. Case of Latvia

2020 ◽  
Vol 24 (2) ◽  
pp. 258-271
Author(s):  
Elīna Račko ◽  
Dagnija Blumberga ◽  
Krišs Spalviņš ◽  
Eglė Marčiulaitienė
Keyword(s):  
Single Cell ◽  
Large Scale ◽  
Oil Production ◽  
Single Cell Oil ◽  
Cultivation Conditions ◽  
Waste Products ◽  
Promising Alternative ◽  
Availability Analysis ◽  
Agroindustrial Waste ◽  
By Products

AbstractSingle cell oil is considered as a promising alternative to fish and vegetable oils. Due to the ability of oleaginous microorganisms to produce significant amounts of polyunsaturated fatty acids such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), single cell oil can be used for human and animal consumption. In this context, marine microorganisms of the class Labyrinthulomycetes are particularly highlighted in scientific literature. In order to clarify the possibilities for the use of microorganisms of the class Labyrinthulomycetes in large-scale single cell oil production, the first step is to gain awareness of the cultivation conditions required for these microorganisms. In addition, to reduce the cost of single cell oil production, it is necessary to identify potential agroindustrial waste products that are suitable for microbiological cultivation of Labyrinthulomycetes. This article examines the potential biotechnological applications of Labyrinthulomycetes in single cell oil production, the cultivation conditions, nutritional requirements, inhibitors and suitable waste products. In addition, an agroindustrial by-product availability analysis has been carried out. The article evaluates agroindustrial by-products based on their availability in Latvia, required pre-treatment, price, shelf life and efficiency.

scholarly journals Valorization of Brewers’ Spent Grain for the Production of Lipids by Oleaginous Yeast

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3052 ◽  
Author(s):  
Alok Patel ◽  
Fabio Mikes ◽  
Saskja Bühler ◽  
Leonidas Matsakas
Keyword(s):  
Lipid Content ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Lipid Concentration ◽  
Microwave Assisted ◽  
Spent Grain ◽  
Synthetic Media ◽  
By Products

Brewers’ spent grain (BSG) accounts for 85% of the total amount of by-products generated by the brewing industries. BSG is a lignocellulosic biomass that is rich in proteins, lipids, minerals, and vitamins. In the present study, BSG was subjected to pretreatment by two different methods (microwave assisted alkaline pretreatment and organosolv) and was evaluated for the liberation of glucose and xylose during enzymatic saccharification trials. The highest amount of glucose (46.45 ± 1.43 g/L) and xylose (25.15 ± 1.36 g/L) were observed after enzymatic saccharification of the organosolv pretreated BSG. The glucose and xylose yield for the microwave assisted alkaline pretreated BSG were 34.86 ± 1.27 g/L and 16.54 ± 2.1 g/L, respectively. The hydrolysates from the organosolv pretreated BSG were used as substrate for the cultivation of the oleaginous yeast Rhodosporidium toruloides, aiming to produce microbial lipids. The yeast synthesized as high as 18.44 ± 0.96 g/L of cell dry weight and 10.41 ± 0.34 g/L lipids (lipid content of 56.45 ± 0.76%) when cultivated on BSG hydrolysate with a C/N ratio of 500. The cell dry weight, total lipid concentration and lipid content were higher compared to the results obtained when grown on synthetic media containing glucose, xylose or mixture of glucose and xylose. To the best of our knowledge, this is the first report using hydrolysates of organosolv pretreated BSG for the growth and lipid production of oleaginous yeast in literature. The lipid profile of this oleaginous yeast showed similar fatty acid contents to vegetable oils, which can result in good biodiesel properties of the produced biodiesel.

Biodiesel-derived crude glycerol as alternative feedstock for single cell oil production by the oleaginous yeast Candida viswanathii Y-E4

2020 ◽  
Vol 145 ◽  
pp. 112103 ◽  
Author(s):  
Mohamed Guerfali ◽  
Ines Ayadi ◽  
Houssem-Eddine Sassi ◽  
Ameni Belhassen ◽  
Ali Gargouri ◽  
...  
Keyword(s):  
Single Cell ◽  
Crude Glycerol ◽  
Oleaginous Yeast ◽  
Oil Production ◽  
Single Cell Oil ◽  
Candida Viswanathii ◽  
Alternative Feedstock
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