Pleurochrysis carterae Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells

In this study, we examined the effect of a hot-water extract of coccolithophore Pleurochrysis carterae on melanogenesis in B16F1 and B16F10 melanoma cells. P. carterae extract inhibited the α-melanocyte-stimulating hormone (α-MSH)-enhanced melanin synthesis in B16F1 melanoma cells. P. carterae also inhibited unstimulated melanin synthesis in B16F10 melanoma cells. Western blotting showed that the P. carterae extract inhibited tyrosinase and microphthalmia-associated transcription factor (MITF) in a dose-dependent manner. The reporter assay also revealed a decline in the tyrosinase promoter activity in the presence of P. carterae extract. Furthermore, quantitative real-time RT-PCR analysis showed that P. carterae extract downregulated the mRNA levels of tyrosinase and MITF. Finally, our study demonstrated that the hot-water extract of P. carterae inhibits melanin synthesis via the down-regulation of MITF mRNA level. Our findings indicate that P. carterae extract could be a possible cosmetic ingredient.

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Effects of methanol extract of Lindera Myrhha on the melanin synthesis of B16f10 melanoma cells

Tạp chí Khoa học ◽

10.54607/hcmue.js.16.3.2459(2019) ◽

2019 ◽

Vol 16 (3) ◽

pp. 174

Author(s):

Le Quynh Loan ◽

Pham Thi Thanh Thuy ◽

Nguyen Luong Hieu Hoa ◽

Le Van Minh ◽

Nguyen Huu Hung ◽

...

Keyword(s):

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Melanoma Cells ◽

Dependent Manner ◽

Melanin Synthesis ◽

B16f10 Melanoma ◽

High Concentration ◽

B16f10 Melanoma Cells

To evaluate the potential of herbal speciesin treatment of hyperpigmentation, this study focuses on the effect of methanol extract of Lindera myrhha on the melanin synthesis in B16F10 melanoma cells. Lindera myrhha showed low cytotoxicity at even high concentration. The methanol extract of this plant could inhibit the melanin synthesis in the dose dependent manner. At the concentration of 200 μg/ml, it could inhibit 42.58% of melanin formation in the B16F10 melanoma cells. The methanol extract of Lindera myrhha slightly inhibited in vitro mushroom tyrosinase activity (5.45%) at a concentration of 200 μg/ml and had a significant inhibitory effect on cellular tyrosinase activivity (36.35%). This plant also showed high free radical scavenging activity with IC50 = 41 mg/ml. This is the first report on activity of this kind in Lindera myrhha. In conclusion, this plant could be used as an effective whitening ingredient in cosmetic products.

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Hot Water Extract of Mulberry Leaf Ameliorates Hepatic Fat Accumulation and Inflammation in Rats Fed a High-Fat Diet

Current Developments in Nutrition ◽

10.1093/cdn/nzaa045_048 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 415-415

Author(s):

Jibin Kim ◽

Chaemin Kim ◽

Mak-Soon Lee ◽

Hyunmi Ko ◽

Soojin Lee ◽

...

Keyword(s):

High Fat Diet ◽

Water Extract ◽

Density Lipoprotein ◽

Hot Water ◽

Mrna Levels ◽

High Fat ◽

Fat Accumulation ◽

Mulberry Leaf ◽

Hot Water Extract ◽

Hepatic Fat

Abstract Objectives This study was conducted to investigate the effect of mulberry leaf extract on hepatic fat accumulation and inflammation in rats fed a high-fat diet. Methods Male Sprague–Dawley rats were randomly divided into three groups. Each group fed normal diet (NOR), high-fat diet (HF), or HF supplemented with 0.8% (w/w) hot water extract of mulberry leaf (HF + ME) for 14 weeks. Results The mulberry extract (ME) supplementation reduced body weight and white adipose tissues (epididymal, retroperitoneal, and mesenteric) weights. Serum levels of triglyceride (TG), total cholesterol (TC), free fatty acids (FFAs), and low-density lipoprotein cholesterol (LDL-C) were lower, while high-density lipoprotein cholesterol (HDL-C) level was higher in the HF + ME group compared to the HF group. The ME reduced the hepatic total lipid, TG, and TC levels compared to the HF group. The mRNA levels of genes related to fatty acid synthesis, such as CD36, sterol regulatory element binding protein 1c (SREBP-1c), and fatty acid synthase (FAS) were down-regulated by the ME supplementation. In addition, the ME lowered the mRNA levels of pro-inflammatory cytokines, such as tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6), compared to the HF group. The serum TNF-α level of the HF + ME group was significantly lower than that of the HF group. Conclusions These results suggested that the ME attenuated high-fat diet-induced hepatic fat accumulation and inflammation via regulating gene expression related to hepatic lipid metabolism and pro-inflammatory mediators. Therefore, it is postulated that the ME might be useful as a functional food ingredient to prevent obesity-induced hepatic fat accumulation and inflammation. Funding Sources None.

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Kinetics of Mushroom Tyrosinase and Melanogenesis Inhibition byN-Acetyl-pentapeptides

The Scientific World JOURNAL ◽

10.1155/2014/409783 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Ching-Yi Lien ◽

Ching-Yu Chen ◽

Shih-Ting Lai ◽

Chin-Feng Chan

Keyword(s):

Kojic Acid ◽

Lag Time ◽

Melanoma Cells ◽

Dependent Manner ◽

Mushroom Tyrosinase ◽

Inhibitory Effects ◽

B16f10 Melanoma ◽

B16f10 Melanoma Cells ◽

Kinetics Of ◽

Dose Dependent

We investigated the kinetics of 4N-acetyl-pentapeptides, Ac-P1, Ac-P2, Ac-P3, and Ac-P4, regarding inhibition of mushroom tyrosinase activity. The peptides sequences of Ac-P1, Ac-P2, Ac-P3, and Ac-P4 were Ac-RSRFK, Ac-KSRFR, Ac-KSSFR, and Ac-RSRFS, respectively. The 4N-acetyl-pentapeptides were able to reduce the oxidation ofL-DOPA by tyrosinase in a dose-dependent manner. Of the 4N-acetyl-pentapeptides, only Ac-P4 exhibited lag time (80 s) at a concentration of 0.5 mg/mL. The tyrosinase inhibitory effects of Ac-P4 (IC500.29 mg/mL) were more effective than those of Ac-P1, Ac-P2, and Ac-P3, in which IC50s were 0.75 mg/mL, 0.78 mg/mL, and 0.81 mg/mL, respectively. Kinetic analysis demonstrated that all 4N-acetyl-pentapeptides were mixed-type tyrosinase inhibitors. Furthermore, 0.1 mg/mL of Ac-P4 exhibited significant melanogenesis inhibition on B16F10 melanoma cells and was more effective than kojic acid. The melanogenesis inhibition of Ac-P4 was dose-dependent and did not induce any cytotoxicity on B16F10 melanoma cells.

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Inhibitory effects of salidroside and paeonol on tyrosinase activity and melanin synthesis in mouse B16F10 melanoma cells and ultraviolet B-induced pigmentation in guinea pig skin

Phytomedicine ◽

10.1016/j.phymed.2013.04.015 ◽

2013 ◽

Vol 20 (12) ◽

pp. 1082-1087 ◽

Cited By ~ 38

Author(s):

Li-Hua Peng ◽

Shuai Liu ◽

Shen-Yao Xu ◽

Lei Chen ◽

Ying-Hui Shan ◽

...

Keyword(s):

Guinea Pig ◽

Melanoma Cells ◽

Tyrosinase Activity ◽

Ultraviolet B ◽

Melanin Synthesis ◽

Inhibitory Effects ◽

B16f10 Melanoma ◽

Pig Skin ◽

B16f10 Melanoma Cells

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Grape Extract Promoted α-MSH-Induced Melanogenesis in B16F10 Melanoma Cells, Which Was Inverse to Resveratrol

Molecules ◽

10.3390/molecules26195959 ◽

2021 ◽

Vol 26 (19) ◽

pp. 5959

Author(s):

Siqi Zhou ◽

Drira Riadh ◽

Kazuichi Sakamoto

Keyword(s):

Biological Activities ◽

Melanoma Cells ◽

Positive Control ◽

Point Of View ◽

Melanin Synthesis ◽

Related Protein ◽

B16f10 Melanoma ◽

Grape Extract ◽

B16f10 Melanoma Cells ◽

Tyrosinase Related Protein

Melanin is a natural pigment produced by cells to prevent damage caused by ultraviolet radiation. Previously, resveratrol was shown to reduce melanin synthesis. As a natural polyphenol with various biological activities, resveratrol occurs in a variety of beverages and plant foods, such as grapes. Therefore, we investigated whether grape extracts containing resveratrol also had the ability to regulate melanin synthesis. In this study, we used mouse B16F10 melanoma cells as a model for melanin synthesis with the melanogenesis-inducing α-melanocyte-stimulating hormone (α-MSH) as a positive control. Our results confirmed previous reports that resveratrol reduces melanin synthesis by reducing the activity of the rate-limiting enzyme tyrosinase. In contrast, the grape extract could not reduce melanin synthesis, and in fact promoted melanogenesis in the presence of α-MSH. The expression of genes related to melanin synthesis, such as tyrosinase, tyrosinase-related protein-1, tyrosinase-related protein-2, and microphthalmia-associated transcription factor, also supports these phenomena, which means that even in the presence of resveratrol, grape extract will strengthen the function of α-MSH in promoting melanin synthesis. Therefore, these results also provide a point of view for research on cosmetics.

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Lindera obtusiloba BL. 70% EtOH extract induced ERK activation inhibits melanin synthesis in mouse B16F10 melanoma cells

Planta Medica ◽

10.1055/s-0030-1264880 ◽

2010 ◽

Vol 76 (12) ◽

Author(s):

C Bang ◽

S Choung

Keyword(s):

Melanoma Cells ◽

Melanin Synthesis ◽

B16f10 Melanoma ◽

Erk Activation ◽

Etoh Extract ◽

B16f10 Melanoma Cells ◽

Lindera Obtusiloba

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Melanogenesis Effect of 7-acetoxy-4-methylcoumarin in B16F10 Melanoma Cells

Cosmetics ◽

10.3390/cosmetics7040094 ◽

2020 ◽

Vol 7 (4) ◽

pp. 94

Author(s):

Ji-Han Sim ◽

Sung-Chan Jang ◽

Tae-Jin Park ◽

Won-Jae Chi ◽

Seung-Young Kim

Keyword(s):

Melanoma Cells ◽

Hair Follicles ◽

Dependent Manner ◽

B16f10 Melanoma ◽

Melanin Production ◽

B16f10 Cells ◽

B16f10 Melanoma Cells ◽

Diphenyl Tetrazolium Bromide ◽

Synthetic Derivatives ◽

Dose Dependent

The increased interest in anti-whitening dyes has enhanced the research interest to identify efficient melanogenic activators. Melanogenesis is the process of melanin production by melanocytes in the hair follicles and skin, which is mediated by several enzymes, such as microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), tyrosinase-related protein (TRP)-1, and TRP-2. This study investigated the melanogenesis-stimulating effect of 4-Methylumbelliferone (4MUMB) and its synthetic derivatives, 7-acetoxy-4-methylcoumarin (7A4MC) and 4-methylheriniarin (4MH) in B16F10 melanoma cells. The cytotoxicity of these compounds was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, followed by the assessment of the melanin content and the intracellular TYR activity. Finally, the expression levels of the key enzymes involved in melanogenesis were investigated. 7A4MC increased melanin production in B16F10 cells relative to that by 4MUMB and 4MH treated cells in a dose-dependent manner without significant cytotoxicity. Concomitantly, 7A4MC significantly increased TYR activity and enhanced the expression of MITF, which significantly induced the expression of TRP-1, TRP-2, and TYR. Furthermore, 7A4MC stimulated melanogenesis via increased phosphorylation of c-Jun N-terminal kinases (JNK) and reduced phosphorylation of protein kinase B (AKT). These results confirmed the melanogenesis-inducing effects of 7A4MC and indicated its potential use as an anti-hair bleaching agent in cosmetics industries.

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