Tropomyosin Analysis in Foods Using an Electrochemical Immunosensing Approach

2021 ◽  
Vol 5 (1) ◽  
pp. 62
Author(s):  
Ricarda Torre ◽  
Maria Freitas ◽  
Estefanía Costa-Rama ◽  
Henri P. A. Nouws ◽  
Cristina Delerue-Matos
Keyword(s):  
Limit Of Detection ◽  
Polyclonal Antibodies ◽  
Analytical Signal ◽  
Food Samples ◽  
Electrochemical Immunosensor ◽  
Screen Printed Carbon Electrode ◽  
Sandwich Type ◽  
Assay Time ◽  
Electrochemical Immunosensing

A screen-printed carbon electrode was used as the transducer for the development of an electrochemical immunosensor for the determination of tropomyosin (a major shrimp allergen) in food samples. Monoclonal and polyclonal antibodies were used in a sandwich-type immunoassay. The analytical signal was electrochemically obtained using an alkaline phosphatase-labelled secondary antibody and a 3-indoxyl phosphate/silver nitrate substrate. The total assay time was 2 h 50 min and allowed the quantification of tropomyosin between 2.5 and 20 ng mL−1, with a limit of detection of 1.7 ng mL−1 The immunosensor was successfully applied to the analysis of commercial food products.

scholarly journals Development of Highly Sensitive Immunosensor for Clenbuterol Detection by Using Poly(3,4-ethylenedioxythiophene)/Graphene Oxide Modified Screen-Printed Carbon Electrode

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4324 ◽  
Author(s):  
Nurul Talib ◽  
Faridah Salam ◽  
Yusran Sulaiman
Keyword(s):  
Graphene Oxide ◽  
Limit Of Detection ◽  
Food Product ◽  
Electrochemical Immunosensor ◽  
Growth Promoter ◽  
Carbon Electrode ◽  
Electrochemical Assay ◽  
Sensor Platform ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA).

scholarly journals An Impedance Based Electrochemical Immunosensor for Aflatoxin B1 Monitoring in Pistachio Matrices

Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 121
Author(s):  
Michail D. Kaminiaris ◽  
Sophie Mavrikou ◽  
Maria Georgiadou ◽  
Georgia Paivana ◽  
Dimitrios I. Tsitsigiannis ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Limit Of Detection ◽  
Electrochemical Immunosensor ◽  
Aflatoxin M1 ◽  
Biomolecular Recognition ◽  
Effective Prevention ◽  
Upper Limits ◽  
Ethylcarbodiimide Hydrochloride ◽  
Food And Feed

Aflatoxins are highly toxic fungal secondary metabolites that often contaminate food and feed commodities. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was fabricated by immobilizing monoclonal AFB1 antibodies onto a screen-printed gold electrode that was modified with carbo-methyldextran by N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide cross-linking. An electrochemical interfacial modelling of biomolecular recognition was suggested and reasonably interpreted. Impedance technology was employed for the quantitative determination of AFB1. The limit of detection concentration of AFB1 for standard solutions and spiked pistachio samples was 0.5 ng/mL and 1 ng/mL, respectively. The immunosensor was able to successfully determine AFB1 concentrations in the range of 4.56–50.86 ng/mL in unknown pistachio samples. Comparative chromatographic analysis revealed that AFB1 concentrations that were higher than 345 ng/mL were not within the immunosensor’s upper limits of detection. Selectivity studies against Ochratoxin A and Aflatoxin M1 demonstrated that the proposed AFB1 immunosensor was able to differentiate between these other fungal mycotoxins. The novel electrochemical immunosensor approach has the potential for rapid sample screening in a portable, disposable format, thus contributing to the requirement for effective prevention and the control of aflatoxin B1 in pistachios.

scholarly journals Melamine Recognition: Molecularly Imprinted Polymer for Selective and Sensitive Determination of Melamine in Food Samples

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Mohadese Biabani ◽  
Azizollah Nezhadali ◽  
Ahmad Nakhaei ◽  
Hossein Nakhaei
Keyword(s):  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Food Samples ◽  
Quantitative Measurements ◽  
Sensitive Determination ◽  
Burman Design ◽  
Mip Sensor ◽  
Optimized Conditions ◽  
Plackett Burman Design

In this study, a sensitive and selective sensor is constructed to measure the melamine (MEL) using molecular imprinting polymer (MIP) technique. Chemical and electrochemical techniques are used to construct the MIP and quantitative measurements. The constructed sensor was modified with GO-Fe3O4@SiO2 nanocomposite. Screening and optimization of factors are done using statistical methods, including Plackett–Burman design (PBD) and central composite design (CCD). Under the optimized conditions, an MIP sensor showed a linear range from 5.0 × 10−7 to 1.0 × 10−5 M MEL concentration with a correlation coefficient (R2) of 0.9997. The limit of detection was obtained (0.028 µM) with a highly reproducible response (RSD 2.15%, n = 4). The electrochemical sensor showed good results for the determination of MEL in food samples.

scholarly journals Development of Lateral Flow Immunochromatographic Assays Using Colloidal Au Sphere and Nanorods as Signal Marker for the Determination of Zearalenone in Cereals

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 281 ◽  
Author(s):  
Mingfei Pan ◽  
Tianyu Ma ◽  
Jingying Yang ◽  
Shijie Li ◽  
Shengmiao Liu ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Test Strip ◽  
Food Samples ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Rapid Screening ◽  
Colloidal Au ◽  
Short Time

This paper describes the development of lateral flow immunochromatographic assays (ICAs) using colloidal Au sphere (SP) and nanorods (NRs) as signal markers for the determination of zearalenone (ZEN) in cereals. The developed ICAs can detect the analyte ZEN within a short time (10 min), and achieve lower limit of detection (LOD). This is the first time that the AuNRs are used as signal probe in immune test strip for ZEN detection. For colloidal AuSP immunochromatographic analysis (AuSP-ICA), the LODs in solution and spiked cereal sample were 5.0 μg L−1 and 60 μg kg−1, and for AuNRs immunochromatographic analysis (AuNRs-ICA) the two LODs achieved 3.0 μg L−1 and 40 μg kg−1, respectively. These two proposed ICAs have minor cross-reaction to the structural analogs of ZEN, and no cross-reactivity with aflatoxin B1, T-2 toxin, ochratoxin A, deoxynivalenol, fumonisin B1. Both of the developed ICAs can specifically and sensitively detect ZEN in cereals, providing an effective strategy for rapid screening and detection of ZEN in a large number of food samples.

scholarly journals Dry-Reagent Double-Monoclonal Assay for Cystatin C

2010 ◽  
Vol 56 (9) ◽  
pp. 1424-1431 ◽  
Author(s):  
Noora Ristiniemi ◽  
Qiu-Ping Qin ◽  
Alexander Postnikov ◽  
Anders Grubb ◽  
Kim Pettersson
Keyword(s):  
Detection Limit ◽  
Cystatin C ◽  
Polyclonal Antibodies ◽  
Cysteine Proteinase ◽  
Cysteine Proteinase Inhibitor ◽  
Reliable Measurement ◽  
Time Resolved ◽  
Sandwich Type ◽  
Assay Time ◽  
Methodological Evaluation

BACKGROUND Cystatin C is a low molecular weight cysteine proteinase inhibitor whose plasma or serum concentrations have been shown to be better correlated with glomerular filtration rate than serum creatinine concentrations. Routine assays for cystatin C are based on use of polyclonal antibodies and immunoturbidimetric and nephelometric designs. This study aimed to develop a double-monoclonal immunoassay for cystatin C. METHODS We tested functionality of 42 2-site antibody combinations involving 7 monoclonal antibodies with recombinant and plasma cystatin C. We developed a heterogeneous assay using 2 antibodies selected to give the best analytical performance. The assay used a dilution step and was based on a dry-reagent, all-in-one immunoassay concept with time-resolved fluorometry. The assay was performed on an automated immunoanalyzer in single wells that contained all the required assay components. We used heparin-derived plasma samples for methodological evaluation of the assay. RESULTS From a relative epitope map involving 7 cystatin C–specific antibodies, we selected a pair of antibodies for a 2-site sandwich-type dry-reagent assay. Total assay time was 15 min, and 10 μL of a 100-fold diluted sample was used. The analytical detection limit (background + 3SD) and functional detection limit (CV 20%) were 0.01 mg/L and 0.02 mg/L, respectively. Within-run and total assay imprecision were <4.7% and <5.6% (at 0.84–3.2 mg/L), respectively, and plasma recoveries of added cystatin C were 94%–110%. Regression analysis with the Roche particle-enhanced immunoturbidimetric method yielded the following (SD): slope, 1.391 (0.029); y-intercept, −0.152 (0.045) mg/L; Sy⊻x=0.294 mg/L (n=131). CONCLUSIONS The developed assay enables rapid and reliable measurement of cystatin C.

Rapid Determination of Fumonisin B1 in Food Samples by a Clean-Up Tandem Immunoassay Column

2012 ◽  
Vol 488-489 ◽  
pp. 1568-1573 ◽  
Author(s):  
Yan Wang ◽  
Guang He Wu ◽  
Wei Sheng ◽  
Yan Zhang ◽  
Meng Yuan ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Rapid Determination ◽  
Enzymatic Reaction ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Food Samples ◽  
Promising Tool ◽  
Pre Treatment ◽  
Treatment Procedures

An immunochemistry-based assay for non-instrumental simultaneous detection of fumonisins in food was developed. The method was based upon the direct competitive immuno-reaction and the horse radish peroxidase enzymatic reaction. The assay was developed to show a visual detection result, according to a yes/no response to the LOD of fumonisins. The limit of detection (LOD) was 40 μg L-1. The assay could be accomplished within 15 min in all and 4 min for chromogenic substrate application. The fumonisin contaminations in different kinds of food were analyzed by the proposed method and the results were confirmed by ELISA. Avoiding time-consuming reaction steps and complicated pre-treatment procedures, this assay was demonstrated as a promising tool for on-site sample detections.

An ultrasensitive sandwich-type electrochemical immunosensor for the determination of SKBR-3 breast cancer cell using rGO-TPA/FeHCFnano labeled Anti-HCT as a signal tag

2017 ◽  
Vol 243 ◽  
pp. 823-830 ◽  
Author(s):  
Mahmoud Amouzadeh Tabrizi ◽  
Mojtaba Shamsipur ◽  
Reza Saber ◽  
Saeed Sarkar ◽  
Najmeh Zolfaghari
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Electrochemical Immunosensor ◽  
Sandwich Type
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