Rapid Determination of Fumonisin B1 in Food Samples by a Clean-Up Tandem Immunoassay Column

2012 ◽  
Vol 488-489 ◽  
pp. 1568-1573 ◽  
Author(s):  
Yan Wang ◽  
Guang He Wu ◽  
Wei Sheng ◽  
Yan Zhang ◽  
Meng Yuan ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Rapid Determination ◽  
Enzymatic Reaction ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Food Samples ◽  
Promising Tool ◽  
Pre Treatment ◽  
Treatment Procedures

An immunochemistry-based assay for non-instrumental simultaneous detection of fumonisins in food was developed. The method was based upon the direct competitive immuno-reaction and the horse radish peroxidase enzymatic reaction. The assay was developed to show a visual detection result, according to a yes/no response to the LOD of fumonisins. The limit of detection (LOD) was 40 μg L-1. The assay could be accomplished within 15 min in all and 4 min for chromogenic substrate application. The fumonisin contaminations in different kinds of food were analyzed by the proposed method and the results were confirmed by ELISA. Avoiding time-consuming reaction steps and complicated pre-treatment procedures, this assay was demonstrated as a promising tool for on-site sample detections.

scholarly journals Development of Lateral Flow Immunochromatographic Assays Using Colloidal Au Sphere and Nanorods as Signal Marker for the Determination of Zearalenone in Cereals

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 281 ◽  
Author(s):  
Mingfei Pan ◽  
Tianyu Ma ◽  
Jingying Yang ◽  
Shijie Li ◽  
Shengmiao Liu ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Test Strip ◽  
Food Samples ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Rapid Screening ◽  
Colloidal Au ◽  
Short Time

This paper describes the development of lateral flow immunochromatographic assays (ICAs) using colloidal Au sphere (SP) and nanorods (NRs) as signal markers for the determination of zearalenone (ZEN) in cereals. The developed ICAs can detect the analyte ZEN within a short time (10 min), and achieve lower limit of detection (LOD). This is the first time that the AuNRs are used as signal probe in immune test strip for ZEN detection. For colloidal AuSP immunochromatographic analysis (AuSP-ICA), the LODs in solution and spiked cereal sample were 5.0 μg L−1 and 60 μg kg−1, and for AuNRs immunochromatographic analysis (AuNRs-ICA) the two LODs achieved 3.0 μg L−1 and 40 μg kg−1, respectively. These two proposed ICAs have minor cross-reaction to the structural analogs of ZEN, and no cross-reactivity with aflatoxin B1, T-2 toxin, ochratoxin A, deoxynivalenol, fumonisin B1. Both of the developed ICAs can specifically and sensitively detect ZEN in cereals, providing an effective strategy for rapid screening and detection of ZEN in a large number of food samples.

scholarly journals Miniaturized Fluorimetric Method for Quantification of Zinc in Dry Dog Food

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Rute C. Martins ◽  
Ana M. Pereira ◽  
Elisabete Matos ◽  
Luisa Barreiros ◽  
António J. M. Fonseca ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Rapid Determination ◽  
Low Cost ◽  
Food Samples ◽  
Dog Food ◽  
Fluorimetric Assay ◽  
Relative Standard ◽  
The Stability ◽  
Cost Determination

Zinc is an essential trace element for animals in several biological processes, particularly in energy production, and it is acquired from food ingestion. In this context, a microplate-based fluorimetric assay was developed for simple, fast, and low-cost determination of zinc in pet food using 2,2′-((4-(2,7-difluoro-3,6-dihydroxy-4aH-xanthen-9-yl)-3-methoxyphenyl)azanediyl)diacetic acid (FluoZin-1) as fluorescent probe. Several aspects were studied, namely, the stability of the fluorescent product over time, the FluoZin-1 concentration, and the pH of reaction media. The developed methodology provided a limit of detection of 1 μg L−1 in sample acid digests, with a working range of 10 to 200 μg L−1, corresponding to 100–2000 mg of Zn per kg of dry dog food samples. Intraday repeatability and interday repeatability were assessed, with relative standard deviation values < 3.4% (100 μg L−1) and <11.7% (10 μg L−1). Sample analysis indicated that the proposed fluorimetric assay provided results consistent with ICP-MS analysis. These results demonstrated that the developed assay can be used for rapid determination of zinc in dry dog food.

scholarly journals An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer

Biosensors ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 15
Author(s):  
Iulia Rus ◽  
Mihaela Tertiș ◽  
Cristina Barbălată ◽  
Alina Porfire ◽  
Ioan Tomuță ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Electrolyte Solution ◽  
Electrode Material ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Linear Sweep Voltammetry ◽  
Pharmaceutical Formulations ◽  
Promising Tool ◽  
Scan Rate

The aim of this study was to develop a disposable, simple, fast, and sensitive sensor for the simultaneous electrochemical detection of doxorubicin (DOX) and simvastatin (SMV), which could be used in preclinical studies for the development of new pharmaceutical formulations for drug delivery. Firstly, the electrochemical behavior of each molecule was analyzed regarding the influence of electrode material, electrolyte solution, and scan rate. After this, the proper electrode material, electrolyte solution, and scan rate for both active substances were chosen, and a linear sweep voltammetry procedure was optimized for simultaneous detection. Two chronoamperometry procedures were tested, one for the detection of DOX in the presence of SMV, and the other one for the detection of DOX and SMV together. Finally, calibration curves for DOX and SMV in the presence of each other were obtained using both electrochemical methods and the results were compared. The use of amperometry allowed for a better limit of detection (DOX: 0.1 μg/mL; SMV: 0.7 μg/mL) than the one obtained in voltammetry (1.5 μg/mL for both drugs). The limits of quantification using amperometry were 0.5 μg/mL for DOX (dynamic range: 0.5–65 μg/mL) and 2 μg/mL for SMV (dynamic range: 2–65 μg/mL), while using voltammetry 1 μg/mL was obtained for DOX (dynamic range: 1–100 μg/mL) and 5 μg/mL for SMV (dynamic range: 5–100 μg/mL). This detection strategy represents a promising tool for the analysis of new pharmaceutical formulations for targeted drug delivery containing both drugs, whose association was proven to bring benefits in the treatment of cancer.

scholarly journals Rapid Determination of Cadmium Contamination in Lettuce Using Laser-Induced Breakdown Spectroscopy

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2930 ◽  
Author(s):  
Tingting Shen ◽  
Wenwen Kong ◽  
Fei Liu ◽  
Zhenghui Chen ◽  
Jingdong Yao ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Rapid Determination ◽  
Univariate Analysis ◽  
Laser Induced Breakdown Spectroscopy ◽  
Growth Environment ◽  
Breakdown Spectroscopy ◽  
Contamination Degree ◽  
Cd Contamination ◽  
Laser Induced Breakdown

Quick access to cadmium (Cd) contamination in lettuce is important to supervise the leafy vegetable growth environment and market. This study aims to apply laser-induced breakdown spectroscopy (LIBS) technology for fast determination of Cd content and diagnosis of the Cd contamination degree in lettuce. Emission lines Cd II 214.44 nm, Cd II 226.50 nm, and Cd I 228.80 nm were selected to establish the univariate analysis model. Multivariate analysis including partial least squares (PLS) regression, was used to establish Cd content calibration models, and PLS model based on 22 variables selected by genetic algorithm (GA) obtained the best performance with correlation coefficient in the prediction set Rp2 = 0.9716, limit of detection (LOD) = 1.7 mg/kg. K-Nearest Neighbors (KNN) and random forest (RF) were used to analyze Cd contamination degree, and RF model obtained the correct classification rate of 100% in prediction set. The preliminary results indicate LIBS coupled with chemometrics could be used as a fast, efficient and low-cost method to assess Cd contamination in the vegetable industry.

scholarly journals Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 415 ◽  
Author(s):  
Xian Zhang ◽  
Zuohuan Wang ◽  
Yun Fang ◽  
Renjie Sun ◽  
Tong Cao ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Goodness Of Fit ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Quantitative Detection ◽  
Antibody Microarray ◽  
Test Kit ◽  
Microarray Immunoassay

We developed and tested a prototype of an antibody microarray immunoassay for simultaneous quantitative detection of four typical mycotoxins (aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1) in corn samples. The test kit consisted of a nitrocellulose membrane layered with immobilized monoclonal antibodies against mycotoxins. During the assay, the mycotoxin-protein conjugates were biotinylated. The signal detection was enhanced by a combination of the biotin-streptavidin system and enhanced chemiluminescence (ECL). This improved the sensitivity of the assay. Under the optimized conditions, four calibration curves with goodness of fit (R2 > 0.98) were plotted. The results showed that the detection limits for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 were 0.21, 0.19, 0.09, and 0.24 ng/mL, with detection ranges of 0.47–55.69, 0.48–127.11, 0.22–31.36, and 0.56–92.57 ng/mL, respectively. The limit of detection (LOD) of this antibody microarray for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 in corn was 5.25, 4.75, 2.25, and 6 μg/kg, respectively. The recovery rates from the spiked samples were between 79.2% and 113.4%, with coefficient of variation <10%. The results of the analysis of commercial samples for mycotoxins using this new assay and the liquid chromatography-tandem mass spectrometry (LC-MS/MS) were comparable and in good agreement. This assay could also be modified for the simultaneous detection of other multiple mycotoxins, as well as low-weight analytes, hazardous to human health.

scholarly journals Rapid determination of actinides in emergency food samples

2011 ◽  
Vol 292 (1) ◽  
pp. 339-347 ◽  
Author(s):  
Sherrod L. Maxwell ◽  
Brian K. Culligan ◽  
Angel Kelsey-Wall ◽  
Patrick J. Shaw
Keyword(s):  
Rapid Determination ◽  
Food Samples

scholarly journals Rapid Determination of Lysine in Biological Samples by Isocratic Liquid Chromatography

1999 ◽  
Vol 82 (4) ◽  
pp. 809-813 ◽  
Author(s):  
Mamun M Or-Rashid ◽  
Ryoji Onodera ◽  
Shaila Wadud ◽  
Mohamed-Emad A Nasser ◽  
Mohammad R Amin
Keyword(s):  
Liquid Chromatography ◽  
Biological Samples ◽  
Limit Of Detection ◽  
Rapid Determination ◽  
Rumen Fluid ◽  
Uv Detector ◽  
Rumen Microorganisms ◽  
Microbial Nitrogen ◽  
Limit Of Quantitation

Abstract A simple, rapid, and sensitive method was developed for detection and quantitation of lysine (Lys) in various biological samples by isocratic liquid chromatography (LC). Samples containing Lys and other amino acids were derivatized with 9-fluorenylmethyl chloroformate (FMOC-CI). The mobile phase used for isocratic elution was 50 mmol/L sodium acetate buffer (pH 4.20)-acetonitrile (43 + 57, v/v). Lys was detected with a UV detector at 265 nm. The derivatized Lys eluted from a LiChrospher 100 RP-18 (150× 4.0 mm id) column at a retention time of 5.6 min. The limit of detection was 0.73 μmol/L (signal-to-noise [S/N] ratio, 3:1), and the limit of quantitation was 2.37 μmol/L (S/N ratio, 10:1). Lys recoveries from fortified biological samples were &gt;97.5%. Average Lys contents found in rumen fluid samples collected before the morning feeding and at 2.0,4.0, and 6.0 h after feeding were 4.26,3.34,3.58, and 3.82 μmol/L, respectively. The hydrolysate of a sample of mixed rumen microorganisms collected before the morning feeding was determined to contain 1.372 μmol/mg microbial nitrogen in the form of Lys. The Lys concentrations of human plasma, goat plasma, human urine, and goat urine were 140.0, 102.0,58.0, and 32.0 μmol/L, respectively.

Threshold Radioactivity for Bulk Food Samples by Gamma Spectroscopy

1965 ◽  
Vol 48 (1) ◽  
pp. 1-5
Author(s):  
Harry M Yakabe ◽  
Hiram Neilson
Keyword(s):  
Gamma Ray ◽  
Gamma Spectroscopy ◽  
Rapid Determination ◽  
Detection System ◽  
Fission Products ◽  
Food Samples ◽  
Family Of Curves ◽  
Lower Limits ◽  
True Activity

Abstract In the surveillance of bulk food produce by gamma ray spectroscopy for fission products, the activities of the commonly observed radionuclides are frequently in the magnitude of background inherent to the detection system. The problems of determining whether the sample is in fact contaminated, the lower limits of detecting the radionuclides, and the effect of compton smear on the lower limits are discussed. The discussions are based on the modified spectrum stripping method for quantitative analysis of gamma ray spectrum for the following radioisotopes: Cs-137, Zr-95/Nb-95, and K-40. A family of curves are shown for rapid determination of the minimum detectable true activity (AII) of Cs-137.

scholarly journals An Improved Method for Analysis of Cholecalciferol-Treated Baits

1999 ◽  
Vol 82 (4) ◽  
pp. 792-798
Author(s):  
Richard E Mauldin ◽  
John J Johnston ◽  
Craig A Riekena
Keyword(s):  
Extraction Method ◽  
Limit Of Detection ◽  
Rapid Determination ◽  
Liquid Extraction ◽  
Detector Response ◽  
Improved Method ◽  
Standard Curve ◽  
Time Periods ◽  
Precision And Accuracy

Abstract A liquid extraction method is described that permits rapid determination of cholecalciferol (D3) in rodenticidal grain baits. Purified D3 was incubated for various time periods to produce pre-D3. Response ratios (concentration/detector response) for various concentrations of pre-D3 and D3 in solutions permitted generation of a correction factor for direct quantitation of pre-D3 in solutions with a pure D3 standard. The method has equal precision and accuracy, yet is simpler and less time consuming and requires less solvents than widely accepted methods for extracting D3 from grain baits. Recoveries from control oat baits fortified at 0.05 and 0.75 wt% were 100.9 and 98%, respectively. A standard curve for concentrations ranging from 6.4 to 204 μg/mL had an r2 of 0.9999 and an intercept of zero and was linear and proportional. The method limit of detection was 2.0 × 10−4 wt % D3.

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