scholarly journals Comparative Study of the Genetic Diversity of Local Steppe Cattle Breeds from Russia, Kazakhstan and Kyrgyzstan by Microsatellite Analysis of Museum and Modern Samples

Diversity ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 351
Author(s):  
Alexandra S. Abdelmanova ◽  
Veronika R. Kharzinova ◽  
Valeria V. Volkova ◽  
Arsen V. Dotsev ◽  
Alexander A. Sermyagin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Genetic ◽  
Microsatellite Analysis ◽  
Molecular Genetic Study ◽  
Cattle Breeds ◽  
Genetic Components ◽  
Museum Samples ◽  
Consensus Genotypes ◽  
Selection Of ◽  
Allele Pool

The comparative molecular genetic study of museum and modern representatives of cattle breeds can help to elucidate the origin and maintenance of historical genetic components in modern populations. We generated the consensus genotypes for 11 microsatellite loci for 24 museum samples of Kalmyk, Kyrgyz, and Kazakh cattle, dated from the first quarter of the 20th century, and compared them with those of modern Kalmyk, Kyrgyz, and Kazakh white-headed breeds. The level of genetic diversity of the modern Kalmyk and Kyrgyz cattle (uHe = 0.771–0.778) was similar to those observed in the museum samples (uHe = 0.772–0.776), while a visible decrease in genetic variability in the modern Kazakh white-headed breed compared to museum Kazakh cattle was detected (uHe = 0.726 and 0.767, respectively). The PCA plot, FST- and Jost’s D-based networks, and STRUCTURE clustering provided strong evidence of the maintenance of the historical genetic background in modern populations of Kalmyk and Kyrgyz cattle. In spite of the allele pool of Kazakh white-headed cattle having undergone great changes compared to the museum Kazakh cattle, several animals still carry the visible aspect of the historical genetic components. Our results can be used for the selection of individuals for the creation of gene banks and may significantly improve the efficiency of conservation programs aimed at preserving genetic diversity in the national genetic resources of cattle.

PSXII-1 Tracing the historical genetic components in turano-mongolian cattle breeds based on the microsatellite analysis of modern and museum samples

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 253-254
Author(s):  
Alexandra S Abdelmanova ◽  
Veronika R Kharzinova ◽  
Valeria Volkova ◽  
Arsen V Dotsev ◽  
Alexander A Sermyagin ◽  
...  
Keyword(s):  
Genetic Structure ◽  
20Th Century ◽  
Animal Husbandry ◽  
Microsatellite Analysis ◽  
Cattle Breeds ◽  
Genetic Components ◽  
Expected Heterozygosity ◽  
Museum Samples ◽  
Consensus Genotypes ◽  
Historical Samples

Abstract In their centuries-long history, the nomads of the south of Russia developed the local cattle breeds, which are well adapted to the harsh steppe conditions. In 20th century, the population size of such cattle breeds was dramatically decreased. At the same time, intensive cross breeding with high-producing transboundary breeds had been practiced on the small remaining part of population. The aim of our study was to trace the historical genetic components in modern populations of local cattle breeds of the turano-mongolian origin. The historical specimens of Kalmyk (n = 10), Kyrgyz (n = 11) and Kazakh (n = 3) cattle dated by the first quarter of the 20th century were derived from the craniological collection of the E.F. Liskun Museum for Animal Husbandry and subjected to the study. The modern representatives of Kalmyk (n = 28), Kyrgyz (n = 20), Kazakh White-headed (n = 30), Hereford (n = 26), and Mongolian (n = 41) cattle were used for comparison. All works with historical samples were performed in dedicated facility of the L.K. Ernst research Center for Animal Husbandry. The samples were genotyped for 11 microsatellite loci (BM1818, BM2113, BM1824, ETH10, ETH225, INRA023, SPS115, TGLA53, TGLA122, TGLA126, and TGLA227). For historical samples, the PCR reactions were carried out in five replicates to determine the consensus genotypes. In total, 132 alleles were identified, including 97 alleles in historical samples and 124 alleles in modern samples. The values of unbiased expected heterozygosity were 0.767–0.776 and 0.653–0.778 for the historical and modern samples, respectively. The STRUCTURE clustering showed the visible differences in genetic structure between the historical and modern populations of Kalmyk, Kyrgyz and Kazakh cattle breeds; however, the historical genetic components were still maintained in modern representatives of all of studied breeds. The research results will be useful for the sustainable breeding and conservation of valuable local genetic resources. The study was funded by the RSF No. 19-76-20012.

scholarly journals Molecular genetic diversity of the Turkish national hazelnut collection and selection of a core set

2017 ◽  
Vol 13 (5) ◽  
Author(s):  
Süleyman Can Öztürk ◽  
Hüseyin İrfan Balık ◽  
Selda Kayalak Balık ◽  
Gökhan Kızılcı ◽  
Ömür Duyar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Genetic ◽  
Molecular Genetic Diversity ◽  
Core Set ◽  
Selection Of

scholarly journals Genetic Diversity of Historical and Modern Populations of Russian Cattle Breeds Revealed by Microsatellite Analysis

Genes ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 940 ◽  
Author(s):  
Alexandra S. Abdelmanova ◽  
Veronika R. Kharzinova ◽  
Valeria V. Volkova ◽  
Arina I. Mishina ◽  
Arsen V. Dotsev ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Average Frequency ◽  
Genetic Distances ◽  
Drop Out ◽  
Cattle Breeds ◽  
Polymorphic Microsatellite ◽  
Consensus Genotypes ◽  
The 19Th Century ◽  
Allelic Drop Out ◽  
Historical Samples

Analysis of ancient and historical DNA has great potential to trace the genetic diversity of local cattle populations during their centuries-long development. Forty-nine specimens representing five cattle breeds (Kholmogor, Yaroslavl, Great Russian, Novgorod, and Holland), dated from the end of the 19th century to the first half of the 20th century, were genotyped for nine polymorphic microsatellite loci. Using a multiple-tube approach, we determined the consensus genotypes of all samples/loci analysed. Amplification errors, including allelic drop-out (ADO) and false alleles (FA), occurred with an average frequency of 2.35% and 0.79%, respectively. A significant effect of allelic length on ADO rate (r2 = 0.620, p = 0.05) was shown. We did not observe significant differences in genetic diversity among historical samples and modern representatives of Kholmogor and Yaroslavl breeds. The unbiased expected heterozygosity values were 0.726–0.774 and 0.708–0.739; the allelic richness values were 2.716–2.893 and 2.661–2.758 for the historical and modern samples, respectively. Analyses of FST and Jost’s D genetic distances, and the results of STRUCTURE clustering, showed the maintenance of a part of historical components in the modern populations of Kholmogor and Yaroslavl cattle. Our study contributes to the conservation of biodiversity in the local Russian genetic resources of cattle.

scholarly journals Molecular genetic diversity in a core of cocoa (Theobroma cacao L.) clones with potential for selection of disease resistance, plant height and fruit production

2016 ◽  
Vol 15 (44) ◽  
pp. 2517-2523
Author(s):  
Batista Lins Adeilson ◽  
Ramos Lawinscky Pabliane ◽  
Marlene Magalhaes Barbosa Antonia ◽  
Amato Gaiotto Fernanda ◽  
Xavier Correa Ronan
Keyword(s):  
Genetic Diversity ◽  
Disease Resistance ◽  
Plant Height ◽  
Theobroma Cacao ◽  
Molecular Genetic ◽  
Fruit Production ◽  
Molecular Genetic Diversity ◽  
Theobroma Cacao L ◽  
Selection Of

scholarly journals PSXII-27 Microsatellite analysis of the historical and modern populations of the Russian local cattle breeds

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 240-241
Author(s):  
Alexandra Abdelmanova ◽  
Arina I Mishina ◽  
Valeria Volkova ◽  
Arsen V Dotsev ◽  
Alexander A Sermyagin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Climatic Conditions ◽  
Microsatellite Analysis ◽  
Science Center ◽  
Holstein Breed ◽  
Cattle Breeds ◽  
Expected Heterozygosity ◽  
Native Cattle ◽  
Private Alleles ◽  
Historical Samples

Abstract During the last centuries, a variety of cattle breeds adapted to the local climatic conditions have been developed in different countries. However, in the 20th century many of them were replaced by high-producing industrial breeds, which can lead to the losses of biodiversity. Our aim was to estimate the genetic diversity of Russian native cattle breeds based on the analysis of historical (museum) and modern samples. Historical samples were derived from the craniological collection of the Museum of Livestock named after E.F. Liskun (Moscow). Forty-six samples including Kholmogor (n = 18), Yaroslavl (n = 19), Saratov (n = 5), Novgorod (n = 2) and Great Russian cattle (n = 2) were subjected to the study. Holland cattle (n = 3) was additionally included in the study due to their possible contribution in forming Russian cattle breeds. The modern samples were presented by Yaroslavl (n = 62) and Kholmogor (n = 177) breeds, and Holstein breed (n = 158) was used as an outgroup. All of the works with historical samples were carried out in the facility of the L.K. Ernst Federal Science Center dedicated for the work with ancient DNA. Nine microsatellite loci (TGLA227, BM2113, ETH10, SPS115, TGLA122, INRA23, TGLA126, ETH225, and BM1824) were successfully amplificated in all samples. We observed the higher variability in historical samples comparing to modern samples: the values of unbiased expected heterozygosity were 0.65–0.85 and 0.70–0.74; the values of rarified allele richness were 2.72–3.11 and 2.63–2.76, respectively. The total number of private alleles was higher in historical populations (16) comparing to modern ones (12). The genetic relationship between historical and modern populations were evaluated. Our results indicate a decrease in genetic diversity in modern populations and a possible loss of valuable allelic combinations. The research results will be useful for development of comprehensive strategies for the conservation of the Russian native genetic resources of cattle. The study was funded by RSF №19-76-20012.

scholarly journals The genetic structure of the herd according to the GDF9, GH genes in Volgograd and Edilbaevsky sheep breeds

2021 ◽  
Vol 14 ◽  
pp. 51-59
Author(s):  
I.F. Gorlov ◽  
◽  
M.I. Slozhenkina ◽  
Y.A. Kolosov ◽  
N.V. Shirokova ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Rural Areas ◽  
Molecular Genetic ◽  
Cartilage Tissue ◽  
Resource Saving ◽  
Molecular Genetic Study ◽  
Differential Growth ◽  
Genetic Studies ◽  
Study Results ◽  
Selection Of

Aim. The study of the genetic structure of sheep of the Volgograd and Edilbaevsky breeds according to the GDF9, GH genes. Materials and Methods. The research was carried out in herds of sheep from leading breeding plants, on the basis of Don State Agrarian University and Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production. Biosamples of skin and cartilage tissue from the auricles were taken from sheep by plucking an area of 1 cm² for molecular genetic studies. Evaluation was performed by PCR-RFLP method. Endonucleases BstHH HaeIII were used to restrict the amplified regions of the GDF9 and GH genes. The presence and frequency of alleles and genotypes for the GDF9, GH genes were determined based on the results of a molecular genetic study. Results. The article is devoted to the study of the genetic structure of sheep of the Volgograd and Edilbaevsky breeds. One of the important resources for creating highly productive animals is the use of marker selection as an additional criterion for the selection and selection of breeding animals. The use of DNA markers for the selection of animals containing desirable alleles of genes of economically valuable traits is one of the new scientific approaches to solve this problem. The development of sheep breeding in our country will allow using the available natural and human resources in rural areas, as well as obtaining high-quality lamb through the use of resource-saving technologies. The aim of the work was to study the genetic structure of sheep of the Volgograd and Edilbaevsky breeds according to the GDF9, GH genes. To perform molecular genetic studies, samples of ear plucks (tissue from the auricle) with an area of 1 cm2 were taken from sheep. The assessment was carried out by PCR-PDRF. In all the studied groups of sheep, A and B allelic variants of the GDF9 gene, alleles A and B of the GH gene were established. The analysis of the data revealed a breed aspect in the distribution of the frequency of alleles and genotypes of the GDF9, GH genes in the populations under consideration. Conclusion. The results of the study of the genetic structure of the populations of sheep of the Volgograd and Edilbaevsky breeds showed that the frequency distribution of alleles and genotypes of the growth hormone (GH) and differential growth factor (GDF9) genes have some features associated with the breed affiliation of the studied sheep.

Challenges in the differential diagnosis of multiple endocrine neoplasia syndrome type 1 with isolated family hyperparathyroidism

2020 ◽  
Vol 98 (3) ◽  
pp. 218-225
Author(s):  
J. A. Krupinova ◽  
N. G. Mokrysheva ◽  
N. Y. Kalinchenko ◽  
A. K. Eremkina ◽  
A. N. Polyakov ◽  
...  
Keyword(s):  
Multiple Endocrine Neoplasia ◽  
Molecular Genetic ◽  
Pancreatic Neuroendocrine Tumor ◽  
Family Type ◽  
Dynamic Monitoring ◽  
Heterozygous Mutation ◽  
Molecular Genetic Study ◽  
Men 1 ◽  
Endocrine Neoplasia

Multiple endocrine neoplasia type 1 (MEN-1) is the most common cause of the hereditary type of primary hyperparathyroidism (PHPT). If a family type of PHPT is suspected, a dynamic monitoring of patients and their close relatives should be carried out throughout their lives. We present a clinical case of a family in which four members of a pedigree were diagnosed with familial isolated hyperparathyroidism (FIHP). The diagnosis was changed to MEN-1, because it appeared that one of the patients had pancreatic neuroendocrine tumor. Molecular genetic study of MEN1 by direct by means of Sanger sequencing revealed that six family members had a new heterozygous mutation in exon 9: s. 1252 G> T p. D418Y.

Microsatellite analysis of genetic diversity in 3 wild populations of Exopalaemon carinicauda

2012 ◽  
Vol 36 (12) ◽  
pp. 1819
Author(s):  
Shuwen JIA ◽  
Ping LIU ◽  
Jian LI ◽  
Jitao LI ◽  
Baoquan GAO ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Analysis ◽  
Exopalaemon Carinicauda ◽  
Wild Populations
Sign in / Sign up

Export Citation Format

Share Document