scholarly journals Microwave Assisted Extraction, Fractionation, Total Phenolic and Flavanoid Estimation and Antioxidant Potential of Annona reticulate Leaves

Proceedings ◽  
2019 ◽  
Vol 41 (1) ◽  
pp. 33
Author(s):  
Sandeep Waghulde ◽  
Mohan K. Kale ◽  
Vijay R. Patil
Keyword(s):  
Crude Extract ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Phenol Content ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Annona Reticulata

The present study focuses on the microwave assisted extraction of Annona reticulata leaves in ethanol. The extract obtained was subjected to column chromatography and the fractionation was done to get different fractions. Preliminary phytochemical screening was done and the fractions were subjected to total phenolic and flavanoid content estimation. The crude extract contained 502 mg GAE/100 g of phenolic content and among the fractions AM8 contained the maximum amount of phenolic content that is 360 mg GAE/100 g. The flavanoid content present in the crude extract of the Annona reticulata was found to be 127 mg QE/100 g. Among all the tested fractions, the fraction AM8 showed more flavanoids i.e., 112 mg QE/100 g and 118 AN/100 g. The antioxidant activity has been studied in-vitro by using DPPH, hydrogen peroxide assay. DPPH, H2O2 and Nitric-oxide scavenging effect of Annona reticulata L. leaf extracts were found to be dose dependant with maximum inhibition at highest concentration. Methanolic extract of the leaves of Annona reticulata Linn. is found to have most potent anti-oxidant activity in DPPH, H2O2 Radical scavenging methods. IC50 value DPPH and H2O2 inhibition of methanolic extract at highest concentration (400 μg/mL) are 62.58 ± 1.15, 68.27 ± 1.05 and 64.01 ± 1.02. The results suggest that all the tested extracts are having antioxidant property, but the methanol is having significantly higher flavonoid and phenol content. Due to presence of higher flavonoid and phenol content in methanol, it may be considered as the fraction with better pharmacological property in comparison to other tested extracts.

scholarly journals Microwave-assisted extraction of phenolic compounds from Moringa oleifera seed as anti-biofouling agents in membrane processes

2018 ◽  
Vol 204 ◽  
pp. 03003 ◽  
Author(s):  
Ni'matul Izza ◽  
Shinta Rosalia Dewi ◽  
Ashried Setyanda ◽  
Agung Sukoyo ◽  
Panggulu Utoro ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Phenolic Content ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Purification Process ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Moringa Oleifera Seed

Moringa seed has known as a coagulant in the water purification process. It is because of the large amount of anti-microbial compounds contained in it. Phenol is one of the most common anti-microbial compounds found in natural materials. The aim of this study was to determine the total phenolic content (TPC) of Moringa seed which was extracted by Microwave-Assisted Extraction (MAE). Moringa seeds were characterized by FTIR and showed that it contained phenol compounds confirmed by specific peak in some areas. Moringa seeds were characterized by FTIR before extracted. Therefore, MAE was performed by variation of solvent ratios (1: 4, 1: 6, 1: 8) and extraction time (2, 3, 4 min). The highest TPC of 41.78 mg GAE / g dw was reached at 1: 8 solvent ratio and 3-min extraction time.

scholarly journals Microwave Assisted Extraction of Papaya Leaves and Investigation on Antioxidant Activity

2021 ◽  
pp. 115-122
Author(s):  
. Shobitharai ◽  
Divya Jyothi ◽  
Swathi Das ◽  
C. M. Sumayya ◽  
A. Thabsheer
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Conventional Extraction

Microwave assisted extraction (MAE) has gained lot of attention due to its advantages such as less solvent consumption, short time period, higher extraction efficiency, therefore serves as better alternative for conventional extraction methods of plant materials. Plant phenolic compounds are important constituents responsible for reducing the oxidative stress that induces tissue damage which is the one of the major causative factors associated with the chronic disease. Papaya plant is a medicinal plant which became popular for the treatment of dengue fever due to its property. Considering the current medicinal importance of the papaya plant, the present study was aimed at microwave assisted extraction of phenolic content from papaya leaf using ethanol, water as solvent and investigate their antioxidant potential. In order to compare the extraction efficiency of phenolic compounds, conventional extraction and microwave assisted extraction method was used to prepare the extracts. Then extracts were subjected to preliminary phytochemical analysis followed by the estimation of total phenolic content by using Folin-Ciocalteu method. Antioxidant activity was investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The alcoholic and aqueous extracts of papaya leaf showed the presence of steroids, alkaloids, saponins, carbohydrates, phenolic compounds by preliminary phytochemical analysis. FTIR spectrum of both aqueous and ethanolic extract showed characteristic peak at 3314.62 cm-1, 1635 cm-1 which provide evidence for presence of phenolic compounds. The total phenolic content of the alcoholic and aqueous leaf extracts from MAE was found to be 43.58mg and 80.58 mg/g papaya leaf powder of the Gallic acid equivalent (GAE), respectively. Aqueous solvent was found to be suitable for extraction of phenolic content from papaya leaf and Microwave assisted extracts showed higher phenolic content and therefore potential antioxidant activity. Therefore, papaya leaf is a good candidate to be used as a natural antioxidant for the treatment of various diseases.

scholarly journals RECOVERY OF THE BIOLOGICAL ACTIVE COMPOUNDS OF Musa Sp. THROUGH MICROWAVE ASSISTED EXTRACTION

2017 ◽  
Vol 18 (2) ◽  
pp. 105-116 ◽  
Author(s):  
Farhan M. Said
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compound ◽  
Phenolic Content ◽  
Irradiation Time ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Liquid Ratio ◽  
Microwave Assisted ◽  
Musa Sp ◽  
Assisted Extraction

The objective of this research was to identify the recovery of the biological active compound of Musa sp. thru microwave assisted extraction (MAE) system. Antioxidant activity and phenolic compound were investigated on the effect of temperature, microwave power, irradiation time and solid to liquid ratio. The extraction process was carried out using only distilled water as an extracting agent. The result showed that, at 70oC fresh Musa sp. extracted 45% higher antioxidant compared to the dried Musa sp. 500 W was the best power of MAE to extract antioxidant and phenolic compound of ripe Musa sp., while for unripe Musa sp. 800 W was the best. The best irradiation time was 90 s, where it extracted almost 90% antioxidant activity of ripe and unripe Musa sp. Almost 480 mg GA/100 g FW of total phenolic content was extracted on the unripe peel. Solid to liquid ratio of 3 : 60 was the best parameter to extract antioxidant and phenolic content of ripe and unripe Musa sp. Overall, antioxidant and phenolic compound were significantly higher in the unripe peel, followed by ripe peel, unripe pulp and ripe pulp.

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