scholarly journals Antioxidant, Scavenging, Reducing, and Anti-Proliferative Activities of Selected Tropical Brown Seaweeds Confirm the Nutraceutical Potential of Spatoglossum asperum

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2482
Author(s):  
Bhakti Tanna ◽  
Babita Choudhary ◽  
Avinash Mishra ◽  
O. P. Chauhan ◽  
Manish Kumar Patel ◽  
...  
Keyword(s):  
Functional Food ◽  
Correlation Study ◽  
Total Phenolic ◽  
Proliferation Inhibition ◽  
Food Ingredient ◽  
Brown Seaweeds ◽  
Multivariate Correlation ◽  
Huh7 Cells ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

Brown seaweeds have shown high potential of bioactivity and provide health benefits as an important functional food ingredient. Therefore, four abundantly growing tropical brown seaweeds—Iyengaria stellata, Spatoglossum asperum, Sargassum linearifolium, and Stoechospermum polypodioides—were collected from the Saurashtra Coast of the Arabian Sea. They were analyzed for metabolite profiling, biochemical activities (including total antioxidant, reducing, scavenging, and anti-proliferative characteristics), and total phenolic and flavonoid contents. A concentration-dependent antioxidant, reducing, and scavenging activities were observed for all four brown seaweeds. The S. asperum and I. stellata extracts showed maximum total antioxidant activity. S. asperum also showed high scavenging and reducing activities compared to other studied brown seaweeds. Further, S. asperum contained high total phenolic and flavonoid content compared to other brown seaweeds collected from the same coast. A multivariate correlation study confirmed a positive correlation between total phenolic and flavonoid contents, and biochemical activities (total antioxidant, scavenging and reducing) for all brown seaweeds. About 35% anti-proliferative activity was observed with S. asperum extract on Huh7 cells; in contrast S. polypodioide showed about 44% proliferation inhibition of Huh7 cells. Similarly, 26% proliferation inhibition of HeLa cells was observed with S. asperum extract. Overall, S. asperum possesses high total flavonoid and phenolic amounts, and showed potential antioxidant, scavenging and reducing characteristics. The study confirmed the nutraceutical potential of S. asperum and that it could be a promising functional food ingredient.

scholarly journals Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of FolkloricLannea coromandelicaUsingIn VitroandIn VivoAssays

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Tekeshwar Kumar ◽  
Vishal Jain
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Intragastric Administration ◽  
Total Antioxidant ◽  
Liver And Kidney ◽  
Mda Content ◽  
Phenolic And Flavonoid

The aim of this study was to determine the impending antioxidant properties of different extracts of crude methanolic extract (CME) of leaves ofLannea coromandelica(L. coromandelica) and its two ethyl acetate (EAF) and aqueous (AqF) subfractions by employing various establishedin vitrosystems and estimation of total phenolic and flavonoid content. The results showed that extract and fractions possessed strong antioxidant activityin vitroand among them, EAF had the strongest antioxidant activity. EAF was confirmed for its highest phenolic content, total flavonoid contents, and total antioxidant capacity. The EAF was found to show remarkable scavenging activity on 2,2-diphenylpicrylhydrazyl (DPPH) (EC5063.9 ± 0.64 µg/mL), superoxide radical (EC508.2 ± 0.12 mg/mL), and Fe2+chelating activity (EC506.2 ± 0.09 mg/mL). Based on ourin vitroresults, EAF was investigated forin vivoantioxidant assay. Intragastric administration of the EAF can significantly increase levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels, and decrease malondialdehyde (MDA) content in the liver and kidney of CCl4-intoxicated rats. These new evidences show thatL. coromandelicabared antioxidant activity.

Antioxidant profiling of Hippophae salicifolia growing in sacred forests of Sikkim, India

2011 ◽  
Vol 38 (9) ◽  
pp. 697 ◽  
Author(s):  
Arvind K. Goyal ◽  
Bharat C. Basistha ◽  
Arnab Sen ◽  
Sushil K. Middha
Keyword(s):  
Antioxidant Activity ◽  
Sea Buckthorn ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Hippophae Salicifolia ◽  
Total Antioxidant ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Phenolic And Flavonoid

Hippophae commonly known as sea-buckthorn, is a versatile plant with multipurpose uses including the control of soil erosion, biological nitrogen fixation and medicinal properties. In India, two species of sea-buckthorn; namely, Hippophae salicifolia D. Don and Hippophae rhamnoides L., are very common. H. salicifolia has a shrub-to-tree habit and is restricted to the Himalayan region, whereas H. rhamnoides is bushy, growing at higher altitude in India, and widely distributed in Europe and Asia. In this study, aqueous, methanol and acetone extracts of various parts of female and male plants of H. salicifolia were evaluated for total antioxidant activity, phenolic and flavonoid contents, and correlations were made. The total phenolic and flavonoid content in all the samples ranged from 99 ± 32 to 1459 ± 53 mg gallic acid equivalent g–1, 135 ± 12 to 707 ± 62 mg quercetin equivalent g–1 of dry extract, respectively. The highest free radical (DPPH) scavenging activity was found in methanol extracts of female bark and male leaf. Methanol extracts of male bark and male leaf showed maximum H2O2 activity at a concentration of 1 mg mL–1. Antioxidant activity (FRAP method) ranged from 0.021 to 1.041 at concentrations ranging from 20 to 200 µg mL–1 with a reducing capacity of extracts as water > methanol > acetone. Strong correlation (P < 0.05) was observed between DPPH scavenging activity and total phenolic content from all the extracts under study.

scholarly journals Antioxidant activity of Trachystemon orientalis (L.) G. Don (Borage) grown and eaten as food in Ordu, Turkey

2015 ◽  
Vol 61 (4) ◽  
pp. 40-51 ◽  
Author(s):  
Melek Col Ayvaz
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Total Antioxidant Capacity ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Synthetic Antioxidants ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

SummaryIntroductıon: Instead of synthetic antioxidants, using of natural products with antioxidant activity is demanded.Objectıve: The aim of this study is to evaluate the antioxidant activity and total phenolic and flavonoid contents of theTrachystemon orientalis(borage) obtained from Ordu.Methods: The water and ethanol extracts of the borage plant were investigated by using severalin vitroantioxidant activity tests such as total antioxidant capacity, O2•−,•OH, DPPH and ABTS radicals scavenging activities, hydrogen peroxide scavenging activity, FRAP, Fe2+chelating and reducing power assays in order to make effective comparisons.Results: Water extract of theT. orientalishad the more phenolic (90 mg GA/g extract) and flavonoid (56.88 mg CT/g extract) contents than ethanol extract. Furthermore, both of the extracts showed strong antioxidant and radical scavenging activities determined by differentin vitromodels. The highest total antioxidant capacity as ascorbic acid equivalents of 244.50 mg/g extract was also calculated for water extract. Generally, a relationship between total phenolic content and antioxidant activity was established. It is also an important finding for an edible food source that the phenolic and flavonoid content and antioxidative activities for water extract was higher than the ethanol extract.Conclusıon:T. orientaliscould be used as a food ingredient instead of synthetic antioxidants and all results will contribute to the recent increase in investigations on using natural products in many areas such as food, pharmacy, alternative medicine and natural therapy.

scholarly journals Antioxidant, Total Phenolic and Flavonoid Content of Selected Nigerian Medicinal Plants

2015 ◽  
Vol 14 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Matthias Onyebuchi Agbo ◽  
Philip Felix Uzor ◽  
Uchenna Nneamaka Akazie Nneji ◽  
Chidozie Uzoma Eze Odurukwe ◽  
Uchenna Basilia Ogbatue ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

Plant phenolics and flavonoids play a great role in scavenging free radicals in the body and act as antioxidants. Thus their determination is sometimes nedded. Total antioxidant capacity, total phenolic and flavonoid contents of the extracts of 10 medicinal plants (Lochnera rosea, Allamanda cathartica, Asplenium platyneuron, Euphorbia prostrate, Baphia nitida, Crotolaria retusa, Zapoteca portoricensis, Platycerium bifurcatum, Mussaenda afzelii and Craterosiphon scandens) from 7 botanical families growing in the tropical rainforest of Nigeria were included in this study. The total antioxidant capacity of the extracts was assessed by using the phosphomolybdate method. The phenolic content was determined by using Folin-Ciocalteau assay, while the total flavonoid was determined by the aluminium chloride colorimetric assay. The results obtained showed that the total antioxidant capacity for all the extracts were in the range of 0.888 ± 0.75 to 0.938 ± 0.00 mg EAA/g. The results showed that E. prostrate, P. bifurcatum and A. platyneuron were found to be the richest source of phenolic (97.77 ± 0.77, 87.62 ± 1.22 and 82.33 ± 0.30 mg GAE/g) while B. nitida and M. afzelii had the least total phenolic content (11.67 ± 0.09 and11.18 ± 0.30 mg GAE/g). The highest total flavonoid content was revealed in P. bifurcatum (648.67 ± 12.3 mg QE) while M. afzelii also had the least total flavonoid content (3.67 ± 0.00 mg QE/g). The ratio of flavonoid to the phenolic in each extract was also determined to ascertain extracts that are rich in flavonoids.Dhaka Univ. J. Pharm. Sci. 14(1): 35-41, 2015 (June)

Identification of the functional food potency of Çalkama: A traditional recipe with edible Mediterranean wild greens from Turkish Cuisine

2021 ◽  
pp. 1-12
Author(s):  
Gokce Altin ◽  
Fatih Bildik ◽  
Kadriye Nur Kasapoğlu ◽  
Seda Genç ◽  
Mahmut Genç ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acid ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Traditional Foods ◽  
Foeniculum Vulgare ◽  
Allium Ampeloprasum ◽  
Total Antioxidant ◽  
Fast Liquid Chromatography ◽  
Phenolic And Flavonoid

BACKGROUND: The phytochemical contents of traditional foods are necessary to further elucidate the impacts of the Mediterranean diet on health. Çalkama is traditional food prepared by using wild green plants including chard (Beta vulgaris var.cicl), wild fennel (Foeniculum vulgare spp), common mellow (Malva sylvestris L), common poppy (Papaver rhoeas L), dock (Rumex spp), common nettle (Urtica dioica L), sow-thistle (Sonchus asper (L) Hill), common stork’s bill (Erodium circutarium (L)L Hér) and wild leek (Allium ampeloprasum L). OBJECTIVE: In this research, the antioxidant activity and the flavonoid and phenolic acid composition were separately analyzed for each plant and Çalkama. METHODS: Total phenolic content, total flavonoid content and total antioxidant capacity of each plant and Çalkama were measured spectrophotometric assays. Ultra-fast liquid chromatography (UFLC) was performed to detect specific flavonoid groups. RESULTS: It was detected that one portion of çalkama (100 g) contained approximately 250 mg of flavonoids. Particularly, quercetin and apigenin contributed to the main flavonoid source and chlorogenic acid was the major phenolic acid in çalkama. CONCLUSIONS: According to our findings, this food can be considered as a good phenolic and flavonoid source which protects its high antioxidant capacity through preparation and cooking processes.

scholarly journals Potentilla rugulosa Nakai Extract Attenuates Bisphenol A-, S- and F-Induced ROS Production and Differentiation of 3T3-L1 Preadipocytes in the Absence of Dexamethasone

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 113 ◽  
Author(s):  
Sun-Il Choi ◽  
Jong Seok Lee ◽  
Sarah Lee ◽  
Wan-Sup Sim ◽  
Young-Cheul Kim ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Functional Food ◽  
Lipid Production ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Ros Production ◽  
Food Ingredient ◽  
Antioxidant Power ◽  
Lipid Metabolism Disorders

Endocrine disrupting chemicals (EDCs) disrupt the physiological metabolism, thus playing an important role in the development of obesity. EDCs, the so-called ‘obesogens’, might predispose some individuals to gain weight. This study investigated the effects of bisphenol A (BPA) and its alternatives (BPS and BPF) on adipocyte differentiation and the effects of the leaves of Potentilla rugulosa Nakai extract (LPE) as a functional food ingredient on obesogen-induced lipid production and adipogenesis in 3T3-L1 cells. The results showed that LPE has high total phenolic and flavonoid contents (77.58 ± 0.57 mg gallic acid equivalents (GAE)/g and 57.31 ± 1.72 mg quercetin equivalents (QE)/g, respectively). In addition, LPE exerted significant antioxidant effects in terms of DPPH radical scavenging activity, reducing power, ferric-ion reducing antioxidant power, and oxygen radical absorbance capacity. BPA, BPS, and BPF increased lipid accumulation, protein expressions of adipogenic transcription factors (PPAR-γ, C/EBP-α, and aP2), and reactive oxygen species (ROS) production in 3T3-L1 cells. However, LPE suppressed the BPA-, BPS-, and BPF-induced effects on adipogenesis. Therefore, LPE has potential as a functional food supplement that can prevent bisphenol-induced lipid metabolism disorders.

scholarly journals The Comparison of Total Phenolics, Total Antioxidant, and Anti-Tyrosinase Activities of Korean Sargassum Species

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Su Hyeon Baek ◽  
Lei Cao ◽  
Seung Jin Jeong ◽  
Hyeung-Rak Kim ◽  
Taek Jeung Nam ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Health Benefits ◽  
Dry Weight ◽  
Tyrosinase Activity ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Antioxidant Capacities ◽  
Total Antioxidant ◽  
Gallic Acid Equivalent ◽  
Phenolic And Flavonoid

Sargassum species, a group of marine brown algae consumed in Asian countries, have shown various health benefits, such as improving the conditions of cardiovascular disease, osteoarthritis, and hypopigmentation. Also, these benefits are associated with their phenolic content and strong antioxidant capacities. However, the antioxidant capacities of different Sargassum species had not been thoroughly explored and compared. Thus, this study aimed to compare the total phenolic contents, total flavonoid contents, total antioxidant capacities, and anti-tyrosine activity of eleven Sargassum species harvested off the Korean coast. The results revealed that the total phenolic content (from 20.57 to 88.97 mg gallic acid equivalent/g dry weight (dw)), flavonoid content (from 22.08 to 82.33 mg quercetin equivalent/g dw), anti-tyrosinase activity (from 13.30 to 126.30 mg kojic acid equivalent/dw), and antioxidant capacities of the 11 Sargassum species had wide ranges. Among them, S. miyabei Yendo and S. hemiphyllum showed the highest total antioxidant capacities while S. miyabei Yendo exhibiting the highest total phenolic and flavonoid contents. The highest anti-tyrosinase activity was seen in S. fillicinum and S. yendoi. Sargahydroqunoic acid and sargachromanol, two alga-derived meroterpenoid compounds with strong antioxidant activity, were detected and quantified in S. miyabei Yendo and S. serratifolium. Our findings guarantee further investigation of the health benefits of Sargassum species and maximize the commercial usage of these species.

scholarly journals Response of cherry tomato seedlings to liquid fertiliser application under water stress 

2018 ◽  
Vol 45 (No. 1) ◽  
pp. 22-28
Author(s):  
Senad Murtic ◽  
Rodoljub Oljaca ◽  
Ivana Koleska ◽  
Lutvija Karic ◽  
Vida Todorovic
Keyword(s):  
Water Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Proline Content ◽  
Total Phenolic ◽  
Cherry Tomato ◽  
Flavonoid Content ◽  
Tomato Seedlings ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

The aim of this study was to examine the impact of different liquid fertilisers on selected physiological parameters in order to evaluate the drought tolerance of cherry tomato seedlings. The following physiological parameters were investigated: total phenolic and flavonoid content, total antioxidant capacity and proline content of leaf extracts. Total phenolic and flavonoid content were determined using the Folin-Ciocalteu and aluminium chloride colorimetric methods, respectively. The ferric-reducing/antioxidant power (FRAP assay) was used to measure the total antioxidant capacity, while proline content was evaluated according to the method of Bates. The contents of proline, total phenolics and flavonoids were significantly higher in the leaves of cherry tomato seedlings exposed to water stress, which suggests that the higher synthesis of these substances by plants represents an important defence mechanism of drought tolerance. The results also indicate that the application of all the used fertilisers in accordance with the manufacturer’s instructions can significantly increase the content of phenol compounds and total antioxidant capacity of plants under normal growth conditions, thus improving survival under subsequent stress.

scholarly journals Antioxidant Activity of Milling Fractions of Selected Cereals

2012 ◽  
Vol 11 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Eva Ivanišová ◽  
Miroslav Ondrejovič ◽  
Stanislav Šilhár
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Frap Assay ◽  
Total Antioxidant ◽  
Total Antioxidant Potential ◽  
Phenolic And Flavonoid

Antioxidant Activity of Milling Fractions of Selected CerealsThe aim of this study was to evaluate antioxidant potential of four milling fractions of selected cereals grew in the year 2009 and 2010. Free radical scavenging activity of samples was measured using DPPH assay and reducing power was determined using FRAP assay. Secondary, total phenolic and flavonoid content of cereal extracts was evaluated. We found that flour fractions (break flour and reduction flour) showed the lower proportion of the total antioxidant potential than bran fractions (fine bran and coarse bran), which was balanced in observed years. Extract from barley had the highest values of antioxidant activity and polyphenol content.

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