scholarly journals The Comparison of Total Phenolics, Total Antioxidant, and Anti-Tyrosinase Activities of Korean Sargassum Species

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Su Hyeon Baek ◽  
Lei Cao ◽  
Seung Jin Jeong ◽  
Hyeung-Rak Kim ◽  
Taek Jeung Nam ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Health Benefits ◽  
Dry Weight ◽  
Tyrosinase Activity ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Antioxidant Capacities ◽  
Total Antioxidant ◽  
Gallic Acid Equivalent ◽  
Phenolic And Flavonoid

Sargassum species, a group of marine brown algae consumed in Asian countries, have shown various health benefits, such as improving the conditions of cardiovascular disease, osteoarthritis, and hypopigmentation. Also, these benefits are associated with their phenolic content and strong antioxidant capacities. However, the antioxidant capacities of different Sargassum species had not been thoroughly explored and compared. Thus, this study aimed to compare the total phenolic contents, total flavonoid contents, total antioxidant capacities, and anti-tyrosine activity of eleven Sargassum species harvested off the Korean coast. The results revealed that the total phenolic content (from 20.57 to 88.97 mg gallic acid equivalent/g dry weight (dw)), flavonoid content (from 22.08 to 82.33 mg quercetin equivalent/g dw), anti-tyrosinase activity (from 13.30 to 126.30 mg kojic acid equivalent/dw), and antioxidant capacities of the 11 Sargassum species had wide ranges. Among them, S. miyabei Yendo and S. hemiphyllum showed the highest total antioxidant capacities while S. miyabei Yendo exhibiting the highest total phenolic and flavonoid contents. The highest anti-tyrosinase activity was seen in S. fillicinum and S. yendoi. Sargahydroqunoic acid and sargachromanol, two alga-derived meroterpenoid compounds with strong antioxidant activity, were detected and quantified in S. miyabei Yendo and S. serratifolium. Our findings guarantee further investigation of the health benefits of Sargassum species and maximize the commercial usage of these species.

scholarly journals Phytochemical Analysis, Antioxidant and Anticancer Potential of Sideritis niveotomentosa: Endemic Wild Species of Turkey

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2420
Author(s):  
Ela Nur Şimşek Şimşek Sezer ◽  
Tuna Uysal
Keyword(s):  
Cell Lines ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Expression Levels ◽  
Phenolic Contents ◽  
Scavenging Effect ◽  
Methanolic Extracts ◽  
Total Antioxidant

Sideritis niveotomentosa Hub. -Mor. is a local endemic species belonging to the Lamiaceae family. In this study, GC/MS analysis, total antioxidant capacity and anticancer effects of different extracts obtained from S. niveotomentosa were investigated comparatively. Total phenolic contents of extracts were determined by the Folin–Ciocalteu method, total flavonoid contents by aluminum chloride method, and also the free radical scavenging activities of the extracts by DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay. The cytotoxic effect of the extracts was studied via MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay on DLD1, HL60 and ARH77 cell lines. Pro-apoptotic gene expression levels were also tested in the most sensitive cell line ARH77 by Real-Time PCR. The expression levels of 4 pro-apoptotic genes, APAF, BAX, CASP3, and HRK were found to be upregulated in ARH77 cells that were treated extracts. Results showed that methanolic extracts contain more phenolic content than acetone extracts, consistent with DPPH results. As a result, Sideritis niveotomentosa extracts, especially methanolic extracts, are rich in phenolic content and have a strong radical scavenging effect. In addition, the extracts showed selective effects on cell lines. This study is pioneering in terms of future studies, and the findings provide hope for future experimentation.

scholarly journals Effects of water stress on phenolic content and antioxidant activity of African nightshades

2017 ◽  
Vol 15 (2) ◽  
pp. 79-95 ◽  
Author(s):  
ODHIAMBO PETER OKELLO ◽  
JOSEPH P. ONYANGO GWEYI ◽  
MILDRED PAULINE NAWIRI ◽  
WINFRED MUSILA
Keyword(s):  
Antioxidant Activity ◽  
Water Stress ◽  
Leaf Area ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Dry Weight ◽  
Total Phenolic ◽  
Stress Levels ◽  
Total Antioxidant ◽  
Solanum Villosum

Okello OP, Gweyi JPO, Nawiri Mp, Musila W. 2017. Effects of water stress on phenolic contents and antioxidant activity of African nightshades. Biofarmasi J Nat Prod Biochem 15: 79-95. This study aimed at mapping out the distribution of different African Nightshade species in Siaya and Kisii Counties of Kenya and to determine water stress effect on total antioxidant capacity and total phenolic content of two selected African nightshade namely, giant nightshade (Solanum scabrum) and black nightshade (Solanum villosum). Before selection of the two varieties, the study involved farmer field visits, mapping of nightshades present and administration of semi-structured questionnaires to farmers to determine the indigenous vegetables being grown, the nightshade species grown and factors affecting their production. The experiments were conducted both in the field and greenhouse conditions. Watering intervals were at 15 cbars, 50 cbars, and 85 cbars. Data on the number of secondary buds, leaf area, shoot height, shoot and root dry weights were gathered. The total antioxidant capacity and the total phenolic content were recorded using DPPH radical scavenging method and Folin-Ciolcalteu method, respectively. The data collected were subjected to ANOVA. In both counties where production was 100% under small scale, Solanum scabrum was the main variety grown is Siaya County (36%) while in Kisii the main variety was Solanum villosum (32%). There were significant differences (P≤0.05) among treatments in leaf area, plant height, shoot biomass, number of secondary buds, leaf and root total phenolic content and leaf and root antioxidant activity. At all stress levels, Solanum scabrum exhibited the tallest plants with a maximum height of 45.17cm at 15cbars, while Solanum villosum had the shortest plants at all stress levels, with the shortest one being recorded at 16.65 cm at 85 cbars. S. scabrum also had the highest root dry weight (7.78g), shoot dry weight (50.78g) and highest leaf area of (304.45cm2). However, Solanum villosum had the highest number of secondary buds at all stress levels with the highest being 24 at 15 cbars. Concerning phytochemicals, Solanum villosum had a higher concentration of both the total phenolics and antioxidant activity in the shoots (46.41g GAE/Kg DM total phenolic content and 52.68% total antioxidant activity). Meanwhile, Solanum scabrum had a higher concentration in the roots (25.06gGAE/Kg DM total phenolic content and 27.18% total antioxidant activity). Water stress cause a decline in all growth parameters but increased phytochemical accumulation in nightshade accessions grown. It is therefore suggested that for better yields, irrigation should be performed at every 15 cbars, however for adequate phytochemical accumulation, the irrigation should be carried out at 50 cbars. Further research to explore and quantify other phytochemical components as affected by different watering regimes need to be undertaken.

scholarly journals Phenolic and flavonoid content in ethanol extract and agro-morphological diversity of Curcuma aeruginosa accessions growing in West Java, Indonesia

2019 ◽  
Vol 20 (3) ◽  
pp. 656-663
Author(s):  
NURUL KHUMAIDA ◽  
MUHAMAD SYUKUR ◽  
MARIA BINTANG ◽  
WARAS NURCHOLIS
Keyword(s):  
Medicinal Plant ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Morphological Diversity ◽  
Dry Weight ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
West Java ◽  
Extract Yield ◽  
Phenolic And Flavonoid

Abstract. Khumaida N, Syukur M, Bintang M, Nurcholis W. 2019. Phenolic and flavonoid content in ethanol extract and agro-morphological diversity of Curcuma aeruginosa accessions growing in West Java, Indonesia. Biodiversitas 20: 656-663. Curcuma aeruginosa is a rhizomatous medicinal plant with beneficial pharmacological activities. The aim of this work was to analyze the agro-morphological, extract yield, and phenolic content of ten C. aeruginosa accessions which were collected from different locations in Indonesia. Cultivation was carried out in the open field in West Java of Indonesia using a completely randomized design. Qualitative and quantitative parameters were used to investigate agro-morphological traits. Total phenolic and total flavonoids contents were determined in ethanol extracts of samples. The plants were phenotypically diverse, in which there were significant variations among the ten C. aeruginosa accessions in number of leaves, plant height, number of shoots, fresh weight of rhizome, and dry weight of rhizome characters. Variability in the total phenolic and total flavonoid contents ranged from 29.08-46.92 mg GAE/g, and 21.31-33.81 mg QE/g, respectively. Six accessions had high phenolic content and extract yield. Therefore, these accessions could be utilized for commercial scale and also showed a high potency for medicinal plant breeding programs.

scholarly journals Antioxidant, Total Phenolic and Flavonoid Content of Selected Nigerian Medicinal Plants

2015 ◽  
Vol 14 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Matthias Onyebuchi Agbo ◽  
Philip Felix Uzor ◽  
Uchenna Nneamaka Akazie Nneji ◽  
Chidozie Uzoma Eze Odurukwe ◽  
Uchenna Basilia Ogbatue ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

Plant phenolics and flavonoids play a great role in scavenging free radicals in the body and act as antioxidants. Thus their determination is sometimes nedded. Total antioxidant capacity, total phenolic and flavonoid contents of the extracts of 10 medicinal plants (Lochnera rosea, Allamanda cathartica, Asplenium platyneuron, Euphorbia prostrate, Baphia nitida, Crotolaria retusa, Zapoteca portoricensis, Platycerium bifurcatum, Mussaenda afzelii and Craterosiphon scandens) from 7 botanical families growing in the tropical rainforest of Nigeria were included in this study. The total antioxidant capacity of the extracts was assessed by using the phosphomolybdate method. The phenolic content was determined by using Folin-Ciocalteau assay, while the total flavonoid was determined by the aluminium chloride colorimetric assay. The results obtained showed that the total antioxidant capacity for all the extracts were in the range of 0.888 ± 0.75 to 0.938 ± 0.00 mg EAA/g. The results showed that E. prostrate, P. bifurcatum and A. platyneuron were found to be the richest source of phenolic (97.77 ± 0.77, 87.62 ± 1.22 and 82.33 ± 0.30 mg GAE/g) while B. nitida and M. afzelii had the least total phenolic content (11.67 ± 0.09 and11.18 ± 0.30 mg GAE/g). The highest total flavonoid content was revealed in P. bifurcatum (648.67 ± 12.3 mg QE) while M. afzelii also had the least total flavonoid content (3.67 ± 0.00 mg QE/g). The ratio of flavonoid to the phenolic in each extract was also determined to ascertain extracts that are rich in flavonoids.Dhaka Univ. J. Pharm. Sci. 14(1): 35-41, 2015 (June)

Phenolic Profile and Antioxidant Capacity of Walnut Extract as Influenced by the Extraction Method and Solvent

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Ion Trandafir ◽  
Sina Cosmulescu ◽  
Violeta Nour
Keyword(s):  
Dry Matter ◽  
Phenolic Content ◽  
Soxhlet Extraction ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phenolic Contents ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Gallic Acid Equivalent

Abstract Total phenolic content, total flavonoid content, antioxidant activity and individual phenolic compounds were assessed in full fat and defatted walnut kernel. For quantification of phenolic fraction of walnut kernels, two different solvents (methanol and ethanol) and two methods of extraction (ultrasonic-assisted extraction and Soxhlet extraction) were tested. Total phenolics, flavonoid content and antioxidant capacity of alcoholic extracts varied depending on the solvent used and extraction methods. Seventeen phenolic compounds were detected and the study provides evidence on high phenolic contents and high antioxidant potential of full fat walnut kernel and defatted walnut kernel. The Soxhlet extraction is the best in terms of the amounts of total phenolic content (2,089.2 mg gallic acid equivalent/100 g dry matter), while the ultrasonic assisted extraction is a fast method but resulted in significantly lower phenolic content (667.3–1,426.8 mg gallic acid equivalent /100 g dry matter). The concentrations of phenolics (especially (+)-catechin hydrate, juglone, vanillic acid, caffeic acid, ferulic acid, sinapic acid, salicylic acid and ellagic acid) are many fold lower in ultrasonic-assisted extraction as compare to the Soxhlet method using the same extraction solvent. The results of this study provide evidence on high phenolic contents and high antioxidant potential of full fat and defatted walnut kernel.

Comparison of total phenolic contents and antioxidant capacities in mint genotypes used as spices / Baharat olarak kullanılan nane genotiplerinin toplam fenolik içerikleri ve antioksidan kapasitelerinin karşılaştırılması

2015 ◽  
Vol 40 (6) ◽  
Author(s):  
Mahfuz Elmastaş ◽  
İsa Telci ◽  
Hüseyin Akşit ◽  
Ramazan Erenler
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Dry Weight ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Herbal Tea ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Antioxidant Agents

AbstractObjective: Mint (Mentha spp.) genotypes used as spices are cultivated in Turkey and used for different purposes including herbal tea, spices, the cosmetics industry, and are used in folk medicine. While mint species have been used in traditional practices during humanity’s long history, there is limited research on the comparison of their antioxidant capacity and phenolic contents. This aim of the research is to compare antioxidant capacity and phenolic contents in mint clones to determine superior genotypes for herbal and spice usage and cultivation.Methods: Antioxidant capacity was evaluated by ferric reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC). Total phenolic content in clones were determined by Folin-Ciocalteu method.Results: Maximum total phenolic content [28.27±3.95 μg gallic acid equivalent (GAE)/g dry weight (DW)] and FRAP activity 577.09±46.02 μmol Trolox equiv/g DW were obtained from Clone 13 (M. spicata), while M. piperita clones were higher for TEAC activity (800.02±1.10 μmol Trolox equiv/g DW). The results were first records for M. villoso nervata. FRAP and TEAC activities selected clones were correlated with total phenolic content (r=0.77; 0.73 respectively).Conclusion: According to the results, it can be suggested that Clone 13, Clone 5, (M. spicata) Clone 3 and Clone 8 (M. piperita) are more suitable for use as spice, herbal tea, and antioxidant agents. The clones must be selected for commercial cultivation by the grower and they can be used as spices and herbal teas.

scholarly journals Phenolic profile and antioxidant activity of the selected edible flowers grown in Poland

2021 ◽  
Vol 25 (2) ◽  
pp. 185-200
Author(s):  
Robert Socha ◽  
Justyna Kałwik ◽  
Lesław Juszczak
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Dry Weight ◽  
Total Phenolic ◽  
Phenolic Profile ◽  
Methanolic Extracts ◽  
Total Antioxidant ◽  
Reducing Activity

Abstract The purpose of this study was to determine phenolic profile and antioxidant activity of the selected edible flowers grown in Poland. A significant variation was observed in the both antioxidant activity and total phenolic content. Marigold flowers were characterized by the highest total phenolic content (89.22 mg GEA/g dry weight). In turn, begonia flowers exhibited the highest total flavonoids and phenolic acids content (21.96 mg QE/g dry weight, and 8.60 mg CAE/g dry weight, respectively). Taking into account the type of flowers, begonia and marigold flowers were the richest in phenolic acids. Caffeic and p-coumaric acids were the most frequent ones in the edible flowers grown in Poland. While gallic and p-coumaric acids were the prevalent ones in terms of their content. The begonia and marigold flowers contained quercetin and kaempferol, while hesperetin and naringenin were present in the chives flowers. The marigold flowers were characterized by a particularly high content of quercetin, and also exhibited the highest total antioxidant activity. The methanolic extracts of marigold and begonia flowers were characterized by the highest antioxidant activity, reducing activity, as well as the highest ability to neutralize free radicals.

scholarly journals Development of a Sauce Using Gymnema sylvestre Leaves

2017 ◽  
Vol 2017 ◽  
pp. 1-5
Author(s):  
O. D. A. N. Perera ◽  
P. Pavitha
Keyword(s):  
Sodium Benzoate ◽  
Bitter Taste ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Gymnema Sylvestre ◽  
Flavonoid Content ◽  
Diverse Range ◽  
Total Antioxidant ◽  
Therapeutic Properties ◽  
Gallic Acid Equivalent

Plant derived products have attracted enormous attention due to their diverse range of biological and therapeutic properties. Gymnema sylvestre is an underutilized medicinal herb that is widely distributed in different parts of the world. It is rich in phytochemicals with antidiabetic and antiobesity properties; however, it is less popular due to its bitter taste. This study was focused on developing a sauce using leaves of Gymnema sylvestre. The most acceptable composition (17.2% water, 4.8% vinegar, 3.2% sweet potato, 2.6% chili, 2.0% sugar, 2.0% cinnamon, 2.0% cardamom, 1.8% ginger, 1.8% garlic, 1.2% onion, 1.2% salt, 0.1% citric acid, and 0.1% sodium benzoate) of the sauce was with 60% leaf extract. Final product contains 73.54% ± 1.68 moisture, 19.92% ± 2.59 carbohydrate, 2.98% ± 0.44 ash, 2.61% ± 0.58 protein, 0.88% ± 0.05 fiber, and 0.05% ± 0.01 fat. The total flavonoid content was 243.67 ± 8.57 µ moles of rutin equivalent, total phenolic content was 65.44 ± 0.51 µ moles of gallic acid equivalent, and % of inhibition of DPPH of total antioxidant capacity was 43.70 ± 0.72. Sauce formulated with 1000 ppm sodium benzoate had six weeks of shelf life. The results showed that development of a sauce from G. sylvestre leaves is an effective way of delivering the health benefits to the potential consumers.

scholarly journals Determination of chemical composition, antibacterial and antioxidant properties of products obtained from carob and honey locust

2018 ◽  
Vol 44 (3) ◽  
pp. 316-322 ◽  
Author(s):  
Hafize Fidan ◽  
Dasha Mihaylova ◽  
Nadezhda Petkova ◽  
Tana Sapoundzhieva ◽  
Anton Slavov ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Total Phenolic ◽  
Dietary Fibers ◽  
Gleditsia Triacanthos ◽  
Honey Locust ◽  
Gallic Acid Equivalent

Abstract Objective The objective of this study was to characterize flours and syrups, obtained from pods of carob (Ceratonia siliqua L.) and honey locust (Gleditsia triacanthos). Method Flours and syrups, produced by carob and honey locust were analyzed for moisture, ash, protein content, dietary fibers, minerals composition, total phenolic content, as well as antibacterial and antioxidant activity. Results and discussion Carob flour contained high amounts of protein (22.56%) and dietary fibres (28.17%), respectively. Dietary fibers in honey locust flour (33.12%) were higher than that of carob flour (28.17%). The total phenolic content of carob flour (4.53±0.08) was lower than this of honey locust (25.31±0.06) (mg gallic acid equivalent [GAE]/g dry weight). Gleditsia triacanthos flour showed higher antioxidant potential – from 127.52±2.43 to 540.28±2.47 μM TE/g dw. Carob syrup in an amount of 0.15 cm3 demonstrated pronounced antibacterial activity against Listeria monocytogenes, Escherichia coli, Salmonella enterica and Staphylococcus aureus, respectively. Conclusion The current study demonstrated that flour and syrup, obtained from carob (C. siliqua L.) and honey locust (G. triacanthos) pods presented products rich of protein and dietary fiber (both above 20%), good sources of antioxidants, especially poliphenolic compounds and minerals (Mg, Fe and Zn).

scholarly journals Physicochemical Characterization and Polyphenolic Content of Beninese Honeys

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Sênan Christa Lokossou ◽  
Fidèle Paul Tchobo ◽  
Hounnankpon Yédomonhan ◽  
Mohamed Mansourou Soumanou
Keyword(s):  
Moisture Content ◽  
Phenolic Content ◽  
Physicochemical Characterization ◽  
Physicochemical Characteristics ◽  
Total Phenolic ◽  
Free Acidity ◽  
Flavonoid Content ◽  
Phenolic Contents ◽  
Polyphenolic Content ◽  
Phenolic And Flavonoid

The physicochemical and phytochemical analyses of honeys (n=50) from Sudanese, Sudano-Guinean, and Guinean areas of Benin were investigated. Results showed that dark amber is the dominant color. Moisture content ranged from 15.50% to 23.50%, and 72% of honeys respected the Codex Alimentarius recommendation. pH varied between 2.87 and 6.15, and free acidity ranged from 9.00 to 39.00 meq/kg. Electrical conductivity varied from 0.37 to 1.43 mS/cm. The content in fructose varied from 21.67% to 94.21%, and proline content ranged between 306.31 and 1187.93 mg/kg. All physicochemical characteristics varied with the areas. A negative correlation was found between pH and moisture content (r=-0.55; p<0.01). A positive correlation was established between pH and conductivity (r=0.79; p<0.01) and between proline and color (r=0.44; p<0.01). Total phenolic content varied between 55.97 and 224.99 mg GAE/100 g, and flavonoid content ranged between 1.43 and 29.81 mg CAE/100 g. Flavonoid was positively correlated with color (r=0.78; p<0.01) and proline (r=0.47; p<0.01). Tukey’s test revealed differences between total phenolic and flavonoid contents of honeys from five areas (p<0.01). In general, Sudanese and Sudano-Guinean honeys showed potential toward therapeutic applications because of their high phenolic contents.

Sign in / Sign up

Export Citation Format

Share Document