scholarly journals The High Content of Ent-11α-hydroxy-15-oxo-kaur- 16-en-19-oic Acid in Adenostemma lavenia (L.) O. Kuntze Leaf Extract: With Preliminary in Vivo Assays

Foods ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 73
Author(s):  
Akie Hamamoto ◽  
Ryosuke Isogai ◽  
Miwa Maeda ◽  
Masumi Hayazaki ◽  
Eito Horiyama ◽  
...  
Keyword(s):  
Water Extract ◽  
Hot Water ◽  
Hair Color ◽  
Hot Water Extract ◽  
Folk Remedy ◽  
Anti Cancer ◽  
Medicinal Properties ◽  
Whole Plants ◽  
Anti Inflammation

Background: Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic acid (11αOH-KA) is a multifunctional biochemical found in some ferns, Pteris semipinnata, and its congeneric species. Although a number of therapeutic applications of 11αOH-KA have been proposed (e.g., anti-cancer, anti-inflammation, and skin whitening), the content of 11αOH-KA in these ferns is not high. Adenostemma lavenia (L.) O. Kuntze, an Asteraceae, has also been reported to contain 11αOH-KA. The decoction (hot water extract) of whole plants of A. lavenia is used as a folk remedy for inflammatory disorders, such as hepatitis and pneumonia, suggesting that 11αOH-KA may be the ingredient responsible for the medicinal properties of this plant. Methods: The anti-melanogenic activities of the water extracts of A. lavenia leaves and Pteris dispar Kunze (a cognate of P. semipinnata) leaves were compared in mouse B16F10 melanoma cells. The amount of 11αOH-KA was measured by using liquid chromatography spectrometry. C57BL/6J mice were treated with the water extract of A. lavenia leaf, and the blood concentration of 11αOH-KA was measured. The in vivo efficacy of the water extract of A. lavenia leaf was evaluated according to tis anti-melanogenic activity by monitoring hair color. Results: Although both the extracts (A. lavenia and P. dispar Kunze) showed high anti-melanogenic activities, only A. lavenia contained a high amount of 11αOH-KA, approximately 2.5% of the dry leaf weight. 11αOH-KA can be purified from A. lavenia leaves in two steps: water extraction followed by chloroform distribution. The treatment of mice with the water extract of A. lavenia leaf suppresses pigmentation in their hairs. Conclusions: Despite the small number of mice examined, the present preliminary result of the suppressed hair pigmentation suggests that the water extract of A. lavenia leaf and the ingredient that is possibly responsible for this—11αOH-KA—are new materials for oral cosmetics. The results may also be helpful in the future development of functional foods and methods to treat patients suffering from hyperpigmentation disorders, such as melasma.

scholarly journals Anti-inflammatory Effect of Onion (Allium cepa) Peel Hot Water Extract in vitro and in vivo

KSBB Journal ◽  
2015 ◽  
Vol 30 (4) ◽  
pp. 148-154
Author(s):  
Bo-Kyeong Kang ◽  
Koth-Bong-Woo-Ri Kim ◽  
Na-Kyung Ahn ◽  
Yeon-Uk Choi ◽  
Min-ji Kim ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Water Extract ◽  
Hot Water ◽  
Hot Water Extract ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Anti-Inflammatory Effects of a Polyphenols-Rich Extract from Tea (Camellia sinensis) Flowers in Acute and Chronic Mice Models

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Bang-Tian Chen ◽  
Wei-Xi Li ◽  
Rong-Rong He ◽  
Yi-Fang Li ◽  
Bun Tsoi ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Water Extract ◽  
Hot Water ◽  
Natural Food ◽  
Liver Inflammation ◽  
Hot Water Extract ◽  
Pharmacological Studies ◽  
Anti Inflammatory ◽  
Necrosis Factor

While beneficial health properties of tea leaves have been extensively studied, less attention is paid to the flowers of tea. In this study, the anti-inflammatory effects of hot water extract of tea (Camellia sinensis) flowers were investigated. Pharmacological studies found that administration of tea flowers extract (TFE) could effectively inhibit croton oil-induced ear edema and carrageenin-induced paw edema. Furthermore, administration of TFE also protected againstPropionibacterium acnes(P. ances) plus lipopolysaccharide-(LPS-) induced liver inflammation by reversing the histologic damage and plasma alanine aminotransferase (ALT) increase. Moreover, the levels of nitric oxide (NO), tumor necrosis factor-(TNF)-αand interleukin-(IL-) 1βmRNA in mouse liver were markedly suppressed after treatment with TFE in mice with immunological liver inflammation. These results indicated that tea flowers had potent anti-inflammatory effects on acute and immunological inflammationin vivo, and may be used as a functional natural food.

scholarly journals Anti-cancer Effect of Hot Water Extract from Mycelium in Germanium-enrichedCordyceps militaris

2017 ◽  
Vol 49 (2) ◽  
pp. 69-78
Author(s):  
Jeong Su Choi ◽  
Ji Hye Heo ◽  
Dae Jin Kim ◽  
Su Min Namkung ◽  
Tae Bok Lee ◽  
...  
Keyword(s):  
Water Extract ◽  
Hot Water ◽  
Hot Water Extract ◽  
Anti Cancer

scholarly journals In Vivo Toxicity and the Antioxidant Activity of the Hot Water Extract of Glechoma Hederacea

2020 ◽  
Author(s):  
Yun-Chin Chung ◽  
Jiunn-Wang Liao ◽  
Kuo-Yuan Li ◽  
Jyun-Kai Jhan ◽  
Su-Tze Chou
Keyword(s):  
Adverse Effect ◽  
Water Extract ◽  
Hot Water ◽  
Oral Toxicity ◽  
Subacute Toxicity ◽  
Hot Water Extract ◽  
Adverse Effect Level ◽  
Effect Level ◽  
Glechoma Hederacea

Abstract Background Glechoma hederacea belongs to the Labiatae family and has many biological effects. Our previously in vitro studies, hot water extract of G. hederacea (HWG) possessed antioxidant and anti-inflammatory activities. Also, the Ames test indicated that HWG had no mutagenicity. However, the in vivo toxicity and antioxidant capacity have not been clearly demonstrated. Thus, this study was aimed to evaluate the antioxidant properties and the safety level of HWG by using animal models. Methods The genotoxicity were performed by micronucleus assays in mice. Acute oral toxicity and 28-day repeated feeding toxicity tests were performed via the oral gavage method for Sprague-Dawley (SD) rats. Furthermore, the effect of HWG on the oxidation–antioxidation equilibrium of male rats was also evaluated. Results HWG did not induce an increase in micronucleus ratios in vivo, no acute lethal effect at a maximum tested dose of 5.0 g HWG /kg bw was observed in rats. The 28-day oral toxicity study revealed the no observed adverse effect level (NOAEL) of HWG in rats was 1.0 g/kg bw. The HWG-treatment significantly elevated the vitamin C level and the SOD activity in heart, and increased the vitamin E concentrations in brain. The HWG-treatment maintained the balance of the glutathione level and the activities of catalase and glutathione peroxidase. Besides, the level of lipid peroxidation and plasma of total antioxidant status (TAS) showed that HWG-treated rats were not significantly changed compared with the control group. Conclusions HWG had no genotoxicity, and did not induce acute or subacute toxicity in SD rat. The level of no observed adverse effect level (NOAEL) of HWG rats was 1.0 g/kg bw for subacute toxicity study. HWG possessed antioxidant potential and reduced oxidative stress by improving the antioxidant system in animal.

scholarly journals Anti-Obesity Effect of Hot Water Extract of Barley Sprout through the Inhibition of Adipocyte Differentiation and Growth

Metabolites ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 610
Author(s):  
Myeong-Jin Kim ◽  
Hye-Won Kawk ◽  
Sang-Hyeon Kim ◽  
Hy-Jae Lee ◽  
Ji-Won Seo ◽  
...  
Keyword(s):  
Adipocyte Differentiation ◽  
Morphological Changes ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Hot Water ◽  
Blood Biochemical ◽  
Hot Water Extract ◽  
Model Weight

Barley sprouts are known to have several effective physiological activities. In this study, the anti-obesity effect of a barley sprout hot water extract (BSE) was confirmed. Saponarin was quantitatively analyzed in BSE using HPLC, and the inhibitory effect on 3T3-L1 pre-adipocyte differentiation into adipocytes was confirmed by Oil Red O staining, TG assay, and Western blotting. In addition, the inhibitory effect of BSE on adipocyte growth was confirmed through glucose uptake and lipolysis of adipocytes. C57/BL/6N mice were induced to obesity with a high-fat diet, and BSE was administered to confirm the effect on an animal model. Weight gain, morphological changes in adipose tissue, changes in the food efficiency ratio, and blood biochemical changes were observed, and an improvement effect on fatty liver was confirmed. As a result, the anti-obesity effect of BSE was confirmed in vitro, and it was confirmed that this effect was also effective in vivo and that it could be helpful in the treatment of obesity-related diseases.

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