Anti-inflammatory Effect of Onion (Allium cepa) Peel Hot Water Extract in vitro and in vivo

While beneficial health properties of tea leaves have been extensively studied, less attention is paid to the flowers of tea. In this study, the anti-inflammatory effects of hot water extract of tea (Camellia sinensis) flowers were investigated. Pharmacological studies found that administration of tea flowers extract (TFE) could effectively inhibit croton oil-induced ear edema and carrageenin-induced paw edema. Furthermore, administration of TFE also protected againstPropionibacterium acnes(P. ances) plus lipopolysaccharide-(LPS-) induced liver inflammation by reversing the histologic damage and plasma alanine aminotransferase (ALT) increase. Moreover, the levels of nitric oxide (NO), tumor necrosis factor-(TNF)-αand interleukin-(IL-) 1βmRNA in mouse liver were markedly suppressed after treatment with TFE in mice with immunological liver inflammation. These results indicated that tea flowers had potent anti-inflammatory effects on acute and immunological inflammationin vivo, and may be used as a functional natural food.

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Anti-Obesity Effect of Hot Water Extract of Barley Sprout through the Inhibition of Adipocyte Differentiation and Growth

Metabolites ◽

10.3390/metabo11090610 ◽

2021 ◽

Vol 11 (9) ◽

pp. 610

Author(s):

Myeong-Jin Kim ◽

Hye-Won Kawk ◽

Sang-Hyeon Kim ◽

Hy-Jae Lee ◽

Ji-Won Seo ◽

...

Keyword(s):

Adipocyte Differentiation ◽

Morphological Changes ◽

Water Extract ◽

Inhibitory Effect ◽

Hot Water ◽

Blood Biochemical ◽

Hot Water Extract ◽

Model Weight

Barley sprouts are known to have several effective physiological activities. In this study, the anti-obesity effect of a barley sprout hot water extract (BSE) was confirmed. Saponarin was quantitatively analyzed in BSE using HPLC, and the inhibitory effect on 3T3-L1 pre-adipocyte differentiation into adipocytes was confirmed by Oil Red O staining, TG assay, and Western blotting. In addition, the inhibitory effect of BSE on adipocyte growth was confirmed through glucose uptake and lipolysis of adipocytes. C57/BL/6N mice were induced to obesity with a high-fat diet, and BSE was administered to confirm the effect on an animal model. Weight gain, morphological changes in adipose tissue, changes in the food efficiency ratio, and blood biochemical changes were observed, and an improvement effect on fatty liver was confirmed. As a result, the anti-obesity effect of BSE was confirmed in vitro, and it was confirmed that this effect was also effective in vivo and that it could be helpful in the treatment of obesity-related diseases.

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Anti-Inflammatory Effect of Hot Water Extract of Aronia Fruits in LPS-Stimulated RAW 264.7 Macrophages

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2015.44.1.007 ◽

2015 ◽

Vol 44 (1) ◽

pp. 7-13 ◽

Cited By ~ 8

Author(s):

Hui Yang ◽

Kwang-Hoon Oh ◽

Yung Choon Yoo

Keyword(s):

Water Extract ◽

Hot Water ◽

Raw 264.7 ◽

Raw 264.7 Macrophages ◽

Hot Water Extract ◽

Anti Inflammatory ◽

Inflammatory Effect

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Effects of a Hot-Water Extract of Allium hookeri Roots on Bone Formation in Human Osteoblast-Like MG-63 Cells In Vitro and in Rats In Vivo

Planta Medica ◽

10.1055/s-0042-108733 ◽

2016 ◽

Vol 82 (16) ◽

pp. 1410-1415 ◽

Cited By ~ 10

Author(s):

Heajin Park ◽

Jaehoon Jeong ◽

Hanbit Hyun ◽

Jihye Kim ◽

Haesung Kim ◽

...

Keyword(s):

Bone Formation ◽

Water Extract ◽

Hot Water ◽

Human Osteoblast ◽

Allium Hookeri ◽

Hot Water Extract

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Hot water extract of oriental melon leaf promotes hair growth and prolongs anagen hair cycle: In vivo and in vitro evaluation

Food Science and Biotechnology ◽

10.1007/s10068-016-0080-0 ◽

2016 ◽

Vol 25 (2) ◽

pp. 575-580 ◽

Cited By ~ 1

Author(s):

Long-Quan Pi ◽

Won-Soo Lee ◽

Sung Hee Min

Keyword(s):

Hair Growth ◽

Water Extract ◽

Hot Water ◽

Hair Cycle ◽

In Vitro Evaluation ◽

Oriental Melon ◽

Hot Water Extract ◽

Anagen Hair

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Dipterocarpus tuberculatus Roxb. Ethanol Extract Has Anti-Inflammatory and Hepatoprotective Effects In Vitro and In Vivo by Targeting the IRAK1/AP-1 Pathway

Molecules ◽

10.3390/molecules26092529 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2529

Author(s):

Haeyeop Kim ◽

Woo Seok Yang ◽

Khin Myo Htwe ◽

Mi-Nam Lee ◽

Young-Dong Kim ◽

...

Keyword(s):

Ethanol Extract ◽

Serum Levels ◽

Hepatoprotective Activity ◽

Tnf Α ◽

Anti Inflammatory ◽

Related Proteins ◽

Pro Inflammatory Cytokines ◽

Inflammatory Effect

Dipterocarpus tuberculatus Roxb. has been used traditionally as a remedy for many diseases, especially inflammation. Therefore, we analyzed and explored the mechanism of the anti-inflammatory effect of a Dipterocarpus tuberculatus Roxb. ethanol extract (Dt-EE). Dt-EE clearly and dose-dependently inhibited the expression of pro-inflammatory cytokines such as IL-6, TNF-α, and IL-1β in lipopolysaccharide (LPS)-treated RAW264.7 cells. Also, Dt-EE suppressed the activation of the MyD88/TRIF-mediated AP-1 pathway and the AP-1 pathway related proteins JNK2, MKK4/7, and TAK1, which occurred as a result of inhibiting the kinase activity of IRAK1 and IRAK4, the most upstream factors of the AP-1 pathway. Finally, Dt-EE displayed hepatoprotective activity in a mouse model of hepatitis induced with LPS/D-galactosamine (D-GalN) through decreasing the serum levels of alanine aminotransferase and suppressing the activation of JNK and IRAK1. Therefore, our results strongly suggest that Dt-EE could be a candidate anti-inflammatory herbal medicine with IRAK1/AP-1 inhibitory and hepatoprotective properties.

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Raphanus sativus ameliorates atherogeneic lipid profiles in hypercholesterolemic rats and hypercholesterolemia-associated peroxidative liver damage

JOURNAL OF ADVANCES IN CHEMISTRY ◽

10.24297/jac.v7i3.2374 ◽

2011 ◽

Vol 7 (3) ◽

pp. 1385-1394 ◽

Cited By ~ 1

Author(s):

Mozammel Haque ◽

Jahirul Islam ◽

Asiqur Rahaman ◽

Fowzia Akter Selina ◽

Mohammad Azizur Rahman ◽

...

Keyword(s):

Oxidative Stress ◽

Raphanus Sativus ◽

Disease Risk ◽

Cardiovascular Disease Risk ◽

Water Extract ◽

Density Lipoprotein ◽

Lipid Peroxide ◽

Hot Water

Objective: Raphanus sativus is a hugely used edible root vegetable. We investigated whether the feeding of the Raphanus sativus hot water extract (RSE) ameliorates atherogenic lipid profile and oxidative stress in hypercholesterolemia. Methods: After feeding of the RSE to hypercholesterolemic rats for 6 weeks, the levels of plasma and hepatic total cholesterol (TC), triglyceride (TG), and plasma high density lipoprotein-cholesterol (HDL-C) and fecal TC levels were studied. The effects of RSE on the hepatic enzymes, namely alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), the levels of lipid peroxide (LPO) and liver histology were also evaluated. Results: Hypercholesterolemia increased the levels of TC and TG in the plasma and livers. The levels of ALT, AST and ALP in plasma and LPO in the liver also increased. The dietary RSE, however, significantly ameliorated the above atherogenic lipids and liver enzymes. The RSE significantly reduced the levels of LPO in the liver, suggesting an in vivo protection against of oxidative stress. The RSE also inhibited the in vitro Fenton’s reagent-induced oxidative stress, thus corroborating the in vivo anti-LPO actions of RSE. The levels of hepatic LPO were positively correlated with plasma AST (r=0.76; P <0.05) and ALT (r=0.43; P<0.05) levels. Histologically, the livers of the RSE-fed hypercholesterolemic rats exhibited lesser fatty droplets and reduced inflammatory cells. Conclusion: Finally, R. sativus extract lowers the cardiovascular disease risk factors under hypercholesterolemic situation by increasing the plasma/hepatic clearance of cholesterol and improving the hypercholesterolemia-induced oxidative damage of hepatic tissues.

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Giloy (Tinospora Cordifolia): In Vitro Evaluation of Antibacterial Properties

RA Journal Of Applied Research ◽

10.47191/rajar/v7i6.03 ◽

2021 ◽

Vol 07 (06) ◽

Author(s):

Tulsa Devi ◽

Keyword(s):

Antibacterial Activity ◽

Methanolic Extract ◽

Water Extract ◽

Hot Water ◽

Tinospora Cordifolia ◽

Diffusion Method ◽

Zone Of Inhibition ◽

Hot Water Extract ◽

Agar Well Diffusion Method

Antibiotic resistance has become a global concern and hence, the search for other source of antimicrobials initiated to find a way to control infections in future. The main objective of this paper is to screen Giloy (Tinospora cordifolia) for its antibacterial activity. The stem of Tinospora cordifolia is used to prepare extract for determining it’s in vitro antibacterial activity as per the agar well diffusion method. In the agar well diffusion method 100μl of 24 hr broth culture of bacteria was aseptically and evenly swabbed on Mueller Hinton agar plates. Wells of about 8 mm diameter were aseptically cut using sterile cork-borer. 100 μl of plant extracts of different concentrations were then placed into the separate wells. The plates were incubated at 37 oC for 24hr. Antimicrobial activity of the giloy was determined by measuring the diameter of zone of inhibition. The methanolic extract of Tinospora cordifolia showed 13, 11, 9 and 5 mm zone of inhibition in S. aureus cultures by using 100, 75, 50 and 25 mg/ml concentration, respectively while hot water extract of Tinospora cordifolia showed 14, 12, 10 and 8 mm zone of inhibition for S. aureus by using 100, 75, 50 and 25 mg/ml concentration, respectively and the cold extract of Tinospora cordifolia showed 10, 8, 5 and 0 mm zone of inhibition for S. aureus by using 100, 75, 50 and 25mg/ml concentration, respectively. The methanolic extract of Tinospora cordifolia indicated 12, 10, 6 and 4 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25 mg/ml concentration, respectively and the hot water extract of Tinospora cordifolia showed 16, 14, 12 and 10 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25mg/ml concentration, respectively. The cold water extract of Tinospora cordifolia showed 13, 10, 8, and 5 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25 mg/ml concentration, respectively. It has been observed that Tinospora cordifolia showed very promising results as indicated by the zone of inhibition of bacterial culture through agar well diffusion method that varies from few mm to few cm. This study indicates the in-vitro antibacterial effect of Giloy which needs further validation through in-vivo studies.

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Natural populations of Galphimia spp. attenuates peripheral and central inflammation

10.21203/rs.3.rs-315697/v1 ◽

2021 ◽

Author(s):

Reinier Gesto-Borroto ◽

Gabriela Meneses ◽

Alejandro Espinosa-Cerón ◽

Guillermo Granados ◽

Jacquelynne Cervantes-Torres ◽

...

Keyword(s):

Systemic Inflammation ◽

Natural Populations ◽

Inflammatory Activity ◽

Medicinal Species ◽

Methanolic Extracts ◽

Nitrite Production ◽

Anti Inflammatory ◽

Inflammatory Effect

Abstract The genus Galphimia is widely distributed in Mexico, and is represented by 22 species, including medicinal species. The sedative and anti-inflammatory effects of galphimines produced by the species Galphimia glauca have been documented. Formerly, molecular studies using DNA barcodes demonstrated that nine populations botanically classified as Galphimia glauca belong to four different species of the genus Galphimia, and that only one exhibited the sedative properties; however, all the collected species showed anti-inflammatory activity. Other bioactive compounds like quercetin, galphins, galphimidins and glaucacetalins have been identified from methanolic extracts of plants botanically classified as Galphimia glauca. The aim of this work was to determine the anti-inflammatory activity of methanolic extracts of nine collected Galphimia spp. populations grown in Mexico. The possible modes of action were analyzed by evaluating the inhibition of LPS-induced inflammation processes both in vitro and in vivo. The nine populations were evaluated by an in vitro model using RAW 264.7 murine macrophage cells, and two populations (a galphimine-producing and a non-galphimine-producing population) were selected for the in vivo experiments of systemic inflammation and neuroinflammation in mice. Results suggest that an anti-inflammatory in vitro effect was present in all the studied populations, evidenced by the inhibition of nitrite production. An inhibitory systemic inflammation in mice was exerted by the two analyzed populations. In the neuroinflammation model, the anti-inflammatory effect was demonstrated in methanolic extract of the non-galphimine-producing population. For the populations of Galphimia spp. studied herein, the anti-inflammatory effect could not be correlated to the presence of galphimines.

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