Comparative Effects of Flaxseed Sources on the Egg ALA Deposition and Hepatic Gene Expression in Hy-Line Brown Hens

Healthy diets are necessary for both humans and animals, including poultry. These diets contain various nutrients for maintenance and production in laying hens. Therefore, research was undertaken to explore the efficiency of various dietary flaxseed sources on the n-3 deposition in the egg yolk and gene expression in laying hens. Five dietary groups were analyzed, i.e., (i) a corn-based diet with no flaxseed (FS) as a negative control (NC), (ii) a wheat-based diet supplemented with 10% whole FS without multi-carbohydrase enzymes (MCE) as a positive control (PC), (iii) ground FS supplemented with MCE (FS), (iv) extruded flaxseed meal was supplemented with MCE (EFM), (v) flaxseed oil supplemented with MCE (FSO). Results indicated that egg weight was highest in the NC, FS, EFM, and FSO groups as compared to PC in the 12th week. Egg mass was higher in enzyme supplemented groups as compared to the PC group, but lower than NC. In the 12th week, the HDEP (hen day egg production) was highest in the FS and EFM groups as compared to FSO, PC, and NC. The FCR (feed conversion ratio) was better in enzyme supplemented groups as compared to the PC group. Enzyme addition enhanced the egg quality as compared to PC in the 12th week. The HDL-C (high-density lipoprotein cholesterol) was increased, while LDL-C (low-density lipoprotein cholesterol), VLDL-C (very-low-density lipoprotein cholesterol), TC (total cholesterol), and TG (total triglycerides) were reduced in the enzyme supplemented groups as compared to PC and NC. The FSO deposit more n-3 PUFA and docosahexaenoic acid (DHA) in the egg yolk as compared to FS and EFM groups. The expression of ACOX1, LCPT1, FADS1, FADS2, and ELOV2 genes were upregulated, while PPAR-α was downregulated in the FSO group. The LPL mRNA expression was upregulated in the FS, EFM, and FSO groups as compared to the PC and NC groups. It was inferred that FSO with enzymes at 2.5% is cost-effective, improves the hen performances, upregulated the fatty acid metabolism and β-oxidation genes expression, and efficiently deposits optimal n-3 PUFA in the egg as per consumer’s demand.

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Feeding a definite concentration of eicosapentaenoic and docosahexaenoic fatty acids to laying hens

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.20345 ◽

2019 ◽

Vol 70 (1) ◽

pp. 1393

Author(s):

E. E. NASSEF ◽

A. A. BAKR ◽

A. S. SALAMA

Keyword(s):

Fatty Acids ◽

Egg Production ◽

Laying Hens ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Egg Yolk ◽

Feed Consumption ◽

Egg Mass ◽

Low Density ◽

Epa And Dha

The experiment designed to study the influences of nutritional eicosapentaenoic (EPA) and docosahexaenoic (DHA) fatty acids (FA) on performance, egg yolk fat characteristics and FA profile in laying hens. From 30 to 36 weeks of age, 180 laying hens were allotted randomly to 2 dietary treatments, each of 6 replicates (15 birds for each replicate). The control diet was supplied with soy oil while the experimental diet was supplied with EPA and DHA to create 2 different ratio of n-6 to n-3 FA (18.8:1 and 5:1, respectively). The egg production % was recorded daily. The eggs were weighed to estimate egg mass. Also, feed consumption was recorded daily and the feed conversion ratio (FCR) was estimated. The FA profile of egg yolk was determined in the last week of the experiment. The dietary EPA and DHA resulted in significantly higher egg production (76.89 versus 67.23%), weightier egg mass (42.46 versus 37. 72 g) and lower FCR (2.49 versus 2.72) than the control. Also, supplying the dietary EPA and DHA was reflected in increasing of total polyunsaturated and n-3 FA in the eggs. Moreover, reducing the ratio of n-6 to n-3 FA to 5:1 decreased egg triglycerides, total cholesterol and cholesterol associated with low density lipoprotein and very low density lipoprotein. In conclusion, supplying EPA and DHA in the diet of laying hens positively influences performance, egg yolk FA profile and cholesterol. Practically, EPA and DHA could be used in laying hen diets to improve their performance and enhance public health of egg consumers.

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Comparación de lípidos sanguíneos entre pollos de engorde y gallinas ponedoras

Revista de la Facultad de Medicina Veterinaria y de Zootecnia ◽

10.15446/rfmvz.v65n1.72021 ◽

2018 ◽

Vol 65 (1) ◽

Author(s):

J. H. Osorio ◽

J. D. Flores

Keyword(s):

High Density Lipoprotein ◽

Total Cholesterol ◽

High Density Lipoprotein Cholesterol ◽

Laying Hens ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Serum Levels ◽

Lipoprotein Cholesterol ◽

Low Density ◽

Low Density Lipoprotein Cholesterol

Objective: To compare serum levels of total cholesterol, triglycerides, low density lipoprotein cholesterol and high density lipoprotein cholesterol between broilers and laying hens. Materials and Methods: the present is a cross study, descriptive and analytic. Data was analyzed using simple ANOVA, the program Statgraphics Plus 5.1 was used. The study was performed at Universidad de Caldas in Manizales (Colombia). After fasting, blood from 30 broilers (Cobb 500 line) of 35-day-old and 40 laying hens (Hy-Line W-36 line) of 26-weeks-old. Serum levels of triglycerides, total cholesterol, low density lipoprotein cholesterol and high density lipoprotein cholesterol was measured by enzymatic colorimetric methods, direct method (detergent + N,Nbis (4-sulfobutyl)-m-toluidine) was used for the lipoprotein cholesterol. Results: Between broilers (Cobb 500 line) and (laying hens (Hy-line W-36 line) was significant difference in serum levels of triglycerides and in serum levels of high density lipoprotein cholesterol (P <0.05); serum levels of total cholesterol and serum levels of low density lipoprotein cholesterol, no differences were found (P> 0.05) Conclusions: Despite differences in gender, age, and production system among broilers Cobb 500 line and laying hens Hy-Line W-36, no differences were found between serum total cholesterol and low density lipoprotein cholesterol.

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A novel Mendelian randomization method identifies causal relationships between gene expression and low-density lipoprotein cholesterol levels

10.1101/671537 ◽

2019 ◽

Cited By ~ 2

Author(s):

Adriaan van der Graaf ◽

Annique Claringbould ◽

Antoine Rimbert ◽

Harm-Jan Westra ◽

Yang Li ◽

...

Keyword(s):

Gene Expression ◽

Complex Traits ◽

Mendelian Randomization ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Lipoprotein Cholesterol ◽

Low Density ◽

Causal Relationships ◽

Low Density Lipoprotein Cholesterol ◽

Individual Level

AbstractRobust inference of causal relationships between gene expression and complex traits using Mendelian Randomization (MR) approaches is confounded by pleiotropy and linkage disequilibrium (LD) between gene expression quantitative loci (eQTLs). Here we propose a new MR method, MR-link, that accounts for unobserved pleiotropy and LD by leveraging information from individual-level data. In simulations, MR-link shows false positive rates close to expectation (median 0.05) and high power (up to 0.89), outperforming all other MR methods we tested, even when only one eQTL variant is present. Application of MR-link to low-density lipoprotein cholesterol (LDL-C) measurements in 12,449 individuals and eQTLs summary statistics from whole blood and liver identified 19 genes causally linked to LDL-C. These include the previously functionally validatedSORT1gene, and thePVRL2gene, located in theAPOElocus, for which a causal role in liver was yet unknown. Our results showcase the strength of MR-link for transcriptome-wide causal inferences.

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