scholarly journals Cytological Evaluations of Advanced Generations of Interspecific Hybrids Between Allium cepa and Allium fistulosum Showing Resistance to Stemphylium vesicarium

Genes ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 195 ◽  
Author(s):  
Natalia Kudryavtseva ◽  
Michael J. Havey ◽  
Lowell Black ◽  
Peter Hanson ◽  
Pavel Sokolov ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Interspecific Hybrids ◽  
Molecular Classification ◽  
Allium Fistulosum ◽  
Bulb Onion ◽  
Allium Cepa L ◽  
Disease Resistances ◽  
Interspecific Crossing

Interspecific crossing is a promising approach for introgression of valuable traits to develop cultivars with improved characteristics. Allium fistulosum L. possesses numerous pest resistances that are lacking in the bulb onion (Allium cepa L.), including resistance to Stemphylium leaf blight (SLB). Advanced generations were produced by selfing and backcrossing to bulb onions of interspecific hybrids between A. cepa and A. fistulosum that showed resistance to SLB. Molecular classification of the cytoplasm established that all generations possessed normal (N) male−fertile cytoplasm of bulb onions. Genomic in situ hybridization (GISH) was used to study the chromosomal composition of the advanced generations and showed that most plants were allotetraploids possessing the complete diploid sets of both parental species. Because artificial doubling of chromosomes of the interspecific hybrids was not used, spontaneous polyploidization likely resulted from restitution gametes or somatic doubling. Recombinant chromosomes between A. cepa and A. fistulosum were identified, revealing that introgression of disease resistances to bulb onion should be possible.

scholarly journals The Power of Genomic in situ Hybridization (GISH) in Interspecific Breeding of Bulb Onion (Allium cepa L.) Resistant to Downy Mildew (Peronospora destructor [Berk.] Casp.)

Plants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 36 ◽  
Author(s):  
Ludmila Khrustaleva ◽  
Majd Mardini ◽  
Natalia Kudryavtseva ◽  
Rada Alizhanova ◽  
Dmitry Romanov ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Downy Mildew ◽  
Allium Cepa ◽  
Genomic In Situ Hybridization ◽  
Lethal Gene ◽  
Peronospora Destructor ◽  
Bulb Onion ◽  
Allium Cepa L ◽  
Interspecific Breeding

We exploited the advantages of genomic in situ hybridization (GISH) to monitor the introgression process at the chromosome level using a simple and robust molecular marker in the interspecific breeding of bulb onion (Allium cepa L.) that is resistant to downy mildew. Downy mildew (Peronospora destructor [Berk.] Casp.) is the most destructive fungal disease for bulb onions. With the application of genomic in situ hybridization (GISH) and previously developed DMR1 marker, homozygous introgression lines that are resistant to downy mildew were successfully produced in a rather short breeding time. Considering that the bulb onion is a biennial plant, it took seven years from the F1 hybrid production to the creation of S2BC2 homozygous lines that are resistant to downy mildew. Using GISH, it was shown that three progeny plants of S2BC2 possessed an A. roylei homozygous fragment in the distal region of the long arm of chromosomes 3 in an A. cepa genetic background. Previously, it was hypothesized that a lethal gene(s) was linked to the downy mildew resistance gene. With the molecular cytogenetic approach, we physically mapped more precisely the lethal gene(s) using the homozygous introgression lines that differed in the size of the A. roylei fragments on chromosome 3.

scholarly journals MOLECULAR HYBRIDIZATION STUDIES IN ALLIUM

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 435e-435
Author(s):  
Usha S. Kallemuchikkal ◽  
E.B. Peffley
Keyword(s):  
Triticum Aestivum ◽  
In Situ Hybridization ◽  
New Mexico ◽  
Allium Cepa ◽  
Genomic Dna ◽  
Interspecific Hybrids ◽  
Allium Fistulosum ◽  
Plant Materials ◽  
High Identity

Total genomic DNA was isolated from study plants and was hybridized with 32P-labeled Allium fistulosum `Ishikura' genomic DNA; Southern blots were performed. Plant materials were Allium cepa `New Mexico Yellow Grano', the Allium fistulosum `Heshiko' and `Ishikura', and their F1 interspecific (Allium fistulosum × Allium cepa) hybrids. Sequences with high identity to the labeled DNA hybridized strongly (i.e., A. fistulosum `Ishikura' hybridized most strongly to itself, next with A. fistulosum `Heshiko'). The least hybridization was observed when A. fistulosum `Ishikura' was hybridized with A. cepa `New Mexico Yellow Grano'. Intensity of the signals observed when DNA of the F1 interspecific hybrids was probed with the `Ishikura' DNA was as expected, with the signals intermediate between those of A. fistulosum to A. fistulosum and A. fistulosum to A. cepa. A second study was performed to identify additional cytological markers in Allium. The 5srDNA and NOR genes from Triticum aestivum onto onion chromosomes using in situ hybridization. Evidence of hybridizations are the presence of fluorescing areas on the chromosomes.

Allium cepa L. Allium fistulosum L. Allium ramosum L. Allium sativum L. Amaryllidaceae

2020 ◽  
pp. 159-169
Author(s):  
Narel Y. Paniagua-Zambrana ◽  
Rainer W. Bussmann ◽  
Carolina Romero
Keyword(s):  
Allium Cepa ◽  
Allium Sativum ◽  
Allium Fistulosum ◽  
Allium Cepa L

Identification of a new bioregulator acting in ultralow doses in bulb onion (Allium cepa L.)

2011 ◽  
Vol 47 (4) ◽  
pp. 356-360 ◽  
Author(s):  
O. G. Kulikova ◽  
V. P. Yamskova ◽  
A. P. Il’ina ◽  
D. V. Margasyuk ◽  
A. A. Molyavka ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Ultralow Doses ◽  
Bulb Onion ◽  
Allium Cepa L

The Comparison of Growth and Yield Characteristics among Principal Bulb Onion (Allium Cepa L.) Cultivars in Organic Growing

2015 ◽  
Vol 23 (1) ◽  
pp. 43-58
Author(s):  
Byeong-Gyu Min ◽  
◽  
Sun-Young Lee ◽  
Jin-Seong Moon ◽  
In-Jong Ha ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Growth And Yield ◽  
Bulb Onion ◽  
Allium Cepa L

Audit of histomorphology and immunohistochemistry of the brain tumors: Revisited in context to the WHO 2016 molecular classification

2017 ◽  
Vol 2 (1) ◽  
pp. 15
Author(s):  
Tanushri Mukherjee ◽  
Rajat Dutta ◽  
Joydeep Ghosh
Keyword(s):  
Social Sciences ◽  
Statistical Analysis ◽  
Brain Tumors ◽  
Molecular Classification ◽  
Histopathological Diagnosis ◽  
Treatment Change ◽  
Tumor Patients ◽  
The Brain

<p><span class="Bold">Background:</span><span> The WHO 2016 molecular classification corroborating with the histology has given more significant diagnostic objectivity to the diagnosis of brain tumors and it is more reliable for instituting therapy as the heterogeneity and observer subjectivity are bypassed with the addition of isocitrate dehydrogenase, ATRX, and 1p19q, and other molecular markers. </span><span class="Bold">Aim:</span><span> Our aim is to review the histopathology of diagnosed brain tumors and correlate with immunohistochemical (IHC) findings to note for any disparity to reform the diagnosis in order to benefit the patient and report to the clinician if any treatment change is to be considered. </span><span class="Bold">Materials and Methods:</span><span> This article is based on studies of screening and diagnostic test. A total of 150 brain tumors were retrospectively analyzed. Age, gender, and the tumor histological type and grade were systematically recorded. We compared our histopathological diagnosis before the introduction of the WHO 2016 molecular classification of central nervous system tumors and later after the relevant IHC and fluorescence </span><span class="Italic">in situ</span><span> hybridization studies. </span><span class="Bold">Statistical Analysis:</span><span> The statistical analysis was done by using Statistical Package for Social Sciences version recent for Windows. </span><span class="Bold">Results:</span><span> Out of the total 150 brain tumor patients, 65 were males and 45 were females. About 37 were glial and the rest were in other categories. </span><span class="Bold">Conclusions:</span><span> </span><span lang="en-US">The molecular diagnosis that substantiated with the histomorphology is more objective and beneficial in the treatment of the patients.</span></p>

Screening and incorporation of rust resistance from Allium cepa into bunching onion (Allium fistulosum) via alien chromosome addition

Genome ◽  
2015 ◽  
Vol 58 (4) ◽  
pp. 135-142 ◽  
Author(s):  
Tadayuki Wako ◽  
Ken-ichiro Yamashita ◽  
Hikaru Tsukazaki ◽  
Takayoshi Ohara ◽  
Akio Kojima ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Rust Resistance ◽  
Rust Fungus ◽  
Allium Fistulosum ◽  
Addition Line ◽  
Chromosome Addition ◽  
Allium Cepa L ◽  
Alien Chromosome Addition ◽  
High Level ◽  
Bunching Onion

Bunching onion (Allium fistulosum L.; 2n = 16), bulb onion (Allium cepa L. Common onion group), and shallot (Allium cepa L. Aggregatum group) cultivars were inoculated with rust fungus, Puccinia allii, isolated from bunching onion. Bulb onions and shallots are highly resistant to rust, suggesting they would serve as useful resources for breeding rust resistant bunching onions. To identify the A. cepa chromosome(s) related to rust resistance, a complete set of eight A. fistulosum – shallot monosomic alien addition lines (MAALs) were inoculated with P. allii. At the seedling stage, FF+1A showed a high level of resistance in controlled-environment experiments, suggesting that the genes related to rust resistance could be located on shallot chromosome 1A. While MAAL, multi-chromosome addition line, and hypoallotriploid adult plants did not exhibit strong resistance to rust. In contrast to the high resistance of shallot, the addition line FF+1A+5A showed reproducibly high levels of rust resistance.

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