Metagenomics Assessment of Soil Fertilization on the Chemotaxis and Disease Suppressive Genes Abundance in the Maize Rhizosphere

Soil fertility is a function of the level of organic and inorganic substances present in the soil, and it influences the activities of soil-borne microbes, plant growth performance and a host of other beneficial ecological functions. In this metagenomics study, we evaluated the response of maize microbial functional gene diversity involved in chemotaxis, antibiotics, siderophores, and antifungals producing genes within the rhizosphere of maize plants under compost, inorganic fertilizer, and unfertilized conditions. The results show that fertilization treatments at higher compost manure and lower inorganic fertilizer doses as well as maize plants itself in the unfertilized soil through rhizosphere effects share similar influences on the abundance of chemotaxis, siderophores, antifungal, and antibiotics synthesizing genes present in the samples, while higher doses of inorganic fertilizer and lower compost manure treatments significantly repress these genes. The implication is for a disease suppressive soil to be achieved, soil fertilization with high doses of compost manure fertilizer treatments as well as lower inorganic fertilizer should be used to enrich soil fertility and boost the abundance of chemotaxis and disease suppressive genes. Maize crops also should be planted sole or intercropped with other crops to enhance the rhizosphere effect of these plants in promoting the expression and abundance of these beneficial genes in the soil.

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Soil fertilization affects the abundance and distribution of carbon and nitrogen cycling genes in the maize rhizosphere

10.21203/rs.3.rs-26191/v1 ◽

2020 ◽

Author(s):

Matthew Chekwube Enebe ◽

Olubukola Oluranti Babalola

Keyword(s):

Nitrogen Cycling ◽

Carbon Cycling ◽

Inorganic Fertilizer ◽

Soil Fertilization ◽

Carbon And Nitrogen ◽

Carbon And Nitrogen Cycling ◽

Fertilized Soil ◽

Maize Plants ◽

And Control ◽

Compost Manure

Abstract Soil microbes performs important functions in nitrogen and carbon cycling in the biosphere. Microbial communities in the rhizosphere enhance plants’ health and promote nutrient turnover and cycling in the soil. In this experimental study, we evaluated the fundamental effects of soil fertilization with organic (compost manure) and inorganic fertilizer on the abundances and distribution of carbon and nitrogen cycling genes within the rhizospheric regions of maize plants. Our result showed that maize plants through rhizosphere effects selected and enriched the same functional genes glnA, gltB, gudB involved in nitrogen cycle as do higher compost and lower inorganic fertilizer treatments. This observation was significantly different from those of higher doses of inorganic fertilizer and lower compost manure treated soil. Only alpha amylase encoding genes were selectively enriched by lower compost and higher inorganic fertilized soil. The other treatments only selected peculiar carbon cycling genes in the rhizosphere of maize. Also Actinomycetales are selected by high compost, low inorganic fertilizer and control while Bacillales are promoted by low compost and higher inorganic fertilizer and this indicated that only microbes capable of tolerating the stress of higher dose of inorganic fertilizer will thrive under such condition. Therefore, soil fertilization lower nitrogen gas emission but increases carbon dioxide evolution in the agricultural soil.

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Soil fertilization affects the abundance and distribution of carbon and nitrogen cycling genes in the maize rhizosphere

AMB Express ◽

10.1186/s13568-021-01182-z ◽

2021 ◽

Vol 11 (1) ◽

Cited By ~ 1

Author(s):

Matthew Chekwube Enebe ◽

Olubukola Oluranti Babalola

Keyword(s):

Nitrogen Cycling ◽

Carbon Cycling ◽

Inorganic Fertilizer ◽

High Dose ◽

Soil Fertilization ◽

Carbon And Nitrogen ◽

Carbon And Nitrogen Cycling ◽

Fertilized Soil ◽

Catabolic Genes ◽

Maize Plants

AbstractSoil microbes perform important functions in nitrogen and carbon cycling in the biosphere. Microbial communities in the rhizosphere enhance plants’ health and promote nutrient turnover and cycling in the soil. In this study, we evaluated the effects of soil fertilization with organic and inorganic fertilizers on the abundances and distribution of carbon and nitrogen cycling genes within the rhizosphere of maize plants. Our result showed that maize plants through rhizosphere effects selected and enriched the same functional genes glnA, gltB, gudB involved in nitrogen cycle as do high compost and low inorganic fertilizer treatments. This observation was significantly different from those of high doses of inorganic fertilizer and low compost manure treated soil. Only alpha amylase encoding genes were selectively enriched by low compost and high inorganic fertilized soil. The other treatments only selected xynB (in Cp8), lacZ (Cp4), bglA, pldB, trpA (N2), uidA (N1) and glgC, vanA (Cn0) carbon cycling genes in the rhizosphere of maize. Also Actinomycetales are selected by high compost, low inorganic fertilizer and control. The control was without any fertilization and the soil was planted with maize. Bacillales are also promoted by low compost and high inorganic fertilizer. This indicated that only microbes capable of tolerating the stress of high dose of inorganic fertilizer will thrive under such condition. Therefore, soil fertilization lowers nitrogen gas emission as seen with the high abundance of nitrogen assimilation genes or microbial anabolic genes, but increases carbon dioxide evolution in the agricultural soil by promoting the abundance of catabolic genes involve in carbon cycling.

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Soil fertilization affects the abundance and distribution of carbon and nitrogen cycling genes in the maize rhizosphere

10.21203/rs.3.rs-26191/v2 ◽

2021 ◽

Author(s):

Matthew Chekwube Enebe ◽

Olubukola Oluranti Babalola

Keyword(s):

Nitrogen Cycling ◽

Carbon Cycling ◽

Inorganic Fertilizer ◽

High Dose ◽

Soil Fertilization ◽

Carbon And Nitrogen ◽

Carbon And Nitrogen Cycling ◽

Fertilized Soil ◽

Catabolic Genes ◽

Maize Plants

Abstract Soil microbes perform important functions in nitrogen and carbon cycling in the biosphere. Microbial communities in the rhizosphere enhance plants’ health and promote nutrient turnover and cycling in the soil. In this study, we evaluated the effects of soil fertilization with organic and inorganic fertilizers on the abundances and distribution of carbon and nitrogen cycling genes within the rhizosphere of maize plants. Our result showed that maize plants through rhizosphere effects selected and enriched the same functional genes glnA, gltB, gudB involved in nitrogen cycle as do high compost and low inorganic fertilizer treatments. This observation was significantly different from those of high doses of inorganic fertilizer and low compost manure treated soil. Only alpha amylase encoding genes were selectively enriched by low compost and high inorganic fertilized soil. The other treatments only selected xynB (in Cp8), lacZ (Cp4), bglA, pldB, trpA (N2), uidA (N1) and glgC, vanA (Cn0) carbon cycling genes in the rhizosphere of maize. Also Actinomycetales are selected by high compost, low inorganic fertilizer and control. The control was without any fertilization and the soil was planted with maize. Bacillales are also promoted by low compost and high inorganic fertilizer. This indicated that only microbes capable of tolerating the stress of high dose of inorganic fertilizer will thrive under such condition. Therefore, soil fertilization lowers nitrogen gas emission as seen with the high abundance of nitrogen assimilation genes or microbial anabolic genes, but increases carbon dioxide evolution in the agricultural soil by promoting the abundance of catabolic genes involve in carbon cycling.

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The Influence of Soil Fertilization on the Distribution and Diversity of Phosphorus Cycling Genes and Microbes Community of Maize Rhizosphere Using Shotgun Metagenomics

Genes ◽

10.3390/genes12071022 ◽

2021 ◽

Vol 12 (7) ◽

pp. 1022

Author(s):

Matthew Chekwube Enebe ◽

Olubukola Oluranti Babalola

Keyword(s):

Inorganic Nitrogen ◽

Phosphorus Cycling ◽

Inorganic Fertilizer ◽

Nitrogen Fertilizers ◽

High Dose ◽

Phosphorus Cycle ◽

Plant Materials ◽

Soil Fertilization ◽

Shotgun Metagenomics ◽

Compost Manure

Biogeochemical cycling of phosphorus in the agro-ecosystem is mediated by soil microbes. These microbes regulate the availability of phosphorus in the soil. Little is known about the response of functional traits of phosphorus cycling microbes in soil fertilized with compost manure (derived from domestic waste and plant materials) or inorganic nitrogen fertilizers at high and low doses. We used a metagenomics investigation study to understand the changes in the abundance and distribution of microbial phosphorus cycling genes in agricultural farmlands receiving inorganic fertilizers (120 kg N/ha, 60 kg N/ha) or compost manure (8 tons/ha, 4 tons/ha), and in comparison with the control. Soil fertilization with high level of compost (Cp8) or low level of inorganic nitrogen (N1) fertilizer have nearly similar effects on the rhizosphere of maize plants in promoting the abundance of genes involved in phosphorus cycle. Genes such as ppk involved in polyphosphate formation and pstSABC (for phosphate transportation) are highly enriched in these treatments. These genes facilitate phosphorus immobilization. At a high dose of inorganic fertilizer application or low compost manure treatment, the phosphorus cycling genes were repressed and the abundance decreased. The bacterial families Bacillaceae and Carnobacteriaceae were very abundant in the high inorganic fertilizer (N2) treated soil, while Pseudonocardiaceae, Clostridiaceae, Cytophagaceae, Micromonosporaceae, Thermomonosporaceae, Nocardiopsaceae, Sphaerobacteraceae, Thermoactinomycetaceae, Planococcaceae, Intrasporangiaceae, Opitutaceae, Acidimicrobiaceae, Frankiaceae were most abundant in Cp8. Pyrenophora, Talaromyces, and Trichophyton fungi were observed to be dominant in Cp8 and Methanosarcina, Methanobrevibacter, Methanoculleus, and Methanosphaera archaea have the highest percentage occurrence in Cp8. Moreover, N2 treatment, Cenarchaeum, Candidatus Nitrososphaera, and Nitrosopumilus were most abundant among fertilized soils. Our findings have brought to light the basis for the manipulation of rhizosphere microbial communities and their genes to improve availability of phosphorus as well as phosphorus cycle regulation in agro-ecosystems.

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Soil fertilization synergistically enhances the impact of pollination services in increasing seed yield of sunflower under dryland conditions

The Journal of Agricultural Science ◽

10.1017/s0021859621000514 ◽

2021 ◽

pp. 1-14

Author(s):

Dolapo Bola Adelabu ◽

Emile Bredenhand ◽

Sean van der Merwe ◽

Angelinus Cornelius Franke

Keyword(s):

Soil Fertility ◽

Seed Yield ◽

Synergetic Effect ◽

Insect Pollination ◽

Pollination Services ◽

Degraded Soil ◽

Soil Fertilization ◽

Fertilized Soil ◽

The Impact ◽

Poor Soil

Abstract To exploit the potential of ecological intensification during sunflower cropping, it is crucial to understand the potential synergies between crop management and ecosystem services. We therefore examined the effect of pollination intensification on sunflower yield and productivity under various levels of soil fertilization over two seasons in the eastern Free State, South Africa. We manipulated soil fertility with fertilizer applications and pollination with exclusion bags. We found a synergetic effect between pollination and soil fertilization whereby increasing pollination intensity led to a far higher impact on sunflower yield when the soil had been fertilized. Specifically, the intensification of insect pollination increased seed yield by approximately 0.4 ton/ha on nutrient poor soil and by approximately 1.7 ton/ha on moderately fertilized soil. Our findings suggest that sunflower crops on adequate balanced soil fertility will receive abundant insect pollination and may gain more from both synergies than crops grown in areas with degraded soil fertility.

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Elevation trend in bacterial functional gene diversity decouples from taxonomic diversity

CATENA ◽

10.1016/j.catena.2020.105099 ◽

2021 ◽

Vol 199 ◽

pp. 105099

Author(s):

Dorsaf Kerfahi ◽

Ke Dong ◽

Ying Yang ◽

Hyoki Kim ◽

Koichi Takahashi ◽

...

Keyword(s):

Gene Diversity ◽

Taxonomic Diversity ◽

Functional Gene ◽

Functional Gene Diversity

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Effiency of organic fertilizers for corn

NATURE AND SCIENCE ◽

10.36719/2707-1146/06/72-74 ◽

2021 ◽

Vol 03 (01) ◽

pp. 72-74

Author(s):

Nərgiz Musa qızı Nəsirli ◽

Keyword(s):

Natural Resources ◽

Soil Fertility ◽

Key Words ◽

Quality Indicators ◽

New Technologies ◽

Normal Development ◽

Natural System ◽

Organic Fertilizers ◽

High Doses ◽

Technical Measures

Pollution of the environment with waste is the basis of the degradation of the natural system. To prevent this, it is necessary to protect natural resources as much as possible and organize the recycling of waste. Azerbaijan has sufficient resources to prepare organic fertilizers based on new technologies. The presented article discusses the damage caused to the environment by pollution of lands of Neftchala region. The need for agro-technical measures to restore such areas that have lost their fertility is emphasized. In landscaping research, it is recommended to grow soybeans on such lands and apply high doses of organic fertilizers to ensure their normal development. Key words: soil fertility, corn, nutrients, productivity, quality indicators

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Metagenomics of Marine Actinomycetes: From Functional Gene Diversity to Biodiscovery

Marine OMICS ◽

10.1201/9781315372303-10 ◽

2016 ◽

pp. 165-186 ◽

Cited By ~ 1

Author(s):

Sergey B. Zotchev ◽

Olga N. Sekurova ◽

D. İpek Kurtböke

Keyword(s):

Gene Diversity ◽

Functional Gene ◽

Marine Actinomycetes ◽

Functional Gene Diversity

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Awareness of Integrated Soil Fertility Management Practices in the Savelugu Municipal of the Northern Region of Ghana

Rural Sustainability Research ◽

10.2478/plua-2020-0005 ◽

2020 ◽

Vol 43 (338) ◽

pp. 35-41

Author(s):

Ammal Abukari ◽

Rahamatu Abukari

Keyword(s):

Soil Fertility ◽

Management Practices ◽

Household Size ◽

Sub Saharan Africa ◽

Inorganic Fertilizer ◽

Small Scale ◽

Soil Fertility Management ◽

Appropriate Use ◽

Integrated Soil Fertility Management ◽

Fertility Management

AbstractIn sub-Saharan Africa intensifying small-scale farming is essential in addressing poverty related issues in rural communities and the degradation of natural resources. Integrated Soil Fertility Management (ISFM) are the best practices used to improve the productivity of crops whilst maximizing agronomic efficiency of inputs applied and hence contributing to sustainable intensification. ISFM usually include the appropriate use of inorganic fertilizer and organic resources, good agronomic practices and appropriate use of germplasm. The survey was carried-out on the awareness of Integrated Soil Fertility Management practices in the Savelugu Municipal of Northern Ghana to study the awareness of integrated soil fertility management practices amongst farmers through the administration of questionnaires. A multistage method of sampling was used in selecting thirty (30) respondents randomly from five (5) selected communities namely Jana, Yapalsi, Diari, Nabogu and Gushie to make up a total sample size of 150 respondents. Frequency distribution and percentages were used to represent the data. Correlation analysis was used to test for the relationship between awareness, educational level and household size. The survey showed that majority of the respondents at ages between 21 and 30 years were married and majority with household size of 3 to 5 as well as primary and secondary education. Farming activities were carried-out by hand (80%). Majority of the respondents (43.3%) were informed about ISFM through demonstrations and 20% of the farmers apply inorganic fertilizer. About 85.5% of the respondents were aware of ISFM. The research also revealed that ISFM improves production and supports finances of respondents. The assessment of respondents’ perception of ISFM revealed a positive agreement of the effect of ISFM on soil health as well as improved production. In conclusion, it is thus suggested that it is needful for the involvement of the government on the adoption of ISFM via Non-Governmental Organisations (NGOs) locally and or internationally for a suitable advancement and to guarantee a sustainable environment with a world-wide corporation for improvement.

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