scholarly journals Population Genetic Structures of Puccinia striiformis f. sp. tritici in the Gansu-Ningxia Region and Hubei Province, China

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1712
Author(s):  
Cuicui Wang ◽  
Bingbing Jiang ◽  
Junmin Liang ◽  
Leifu Li ◽  
Yilin Gu ◽  
...  
Keyword(s):  
Fungal Pathogen ◽  
Population Genetic ◽  
Puccinia Striiformis ◽  
Principal Coordinate Analysis ◽  
Pathogen Population ◽  
Simple Sequence Repeat Markers ◽  
Important Region ◽  
Genetic Structures ◽  
Small Overlap ◽  
Simple Sequence

Wheat stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a destructive wheat disease in China. The Gansu–Ningxia region (GN) is a key area for pathogen over-summering in China, and northwestern Hubei (HB) is an important region for pathogen over-wintering, serving as a source of inoculum in spring epidemic regions. The spatiotemporal population genetic structure of Pst in HB and the pathogen population exchanges between GN and HB are important for estimating the risk of interregional epidemics. Here, 567 isolates from GN and HB were sampled from fall 2016 to spring 2018 and were genotyped using simple sequence repeat markers. The genotypic and genetic diversity of Pst subpopulations in HB varied among seasons and locations. Greater genetic diversification levels were found in the spring compared with fall populations using principal coordinate analysis and Bayesian assignments. In total, there were 17 common genotypes among the 208 determined, as shown by a small overlap of genotypes in the principal coordinate analysis and dissimilar Bayesian assignments in both regions, which revealed the limited genotype exchange between the populations of GN and HB.

scholarly journals Population Genetic Structures of Puccinia striiformis in Ningxia and Gansu Provinces of China

Plant Disease ◽  
2013 ◽  
Vol 97 (4) ◽  
pp. 501-509 ◽  
Author(s):  
Junmin Liang ◽  
Qiong Wan ◽  
Yong Luo ◽  
Zhanhong Ma
Keyword(s):  
Gene Flow ◽  
Fungal Pathogen ◽  
Population Genetic ◽  
Fragment Length Polymorphism ◽  
Puccinia Striiformis ◽  
Genotypic Diversity ◽  
Plant Diseases ◽  
Genotype Distribution ◽  
Population Exchange ◽  
Genetic Structures

Wheat stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici, is one of the most destructive plant diseases in China. Gansu and Ningxia Provinces are considered to be the key areas for over-summering of this pathogen in China. In this study, 283 P. striiformis f. sp. tritici isolates were collected in these two provinces. Amplified fragment length polymorphism (AFLP) was used to analyze the population genetics and to infer the chance of population exchanges between different geographic locations and seasons. The genotypic diversity of the Gansu population (0.514) was slightly higher than that of the Ningxia population (0.489). The occurrence of frequent population exchanges between these two regions was observed, showing that 40 AFLP genotypes were shared by the populations of the two provinces. Gene flow between these two regions in autumn and spring subpopulations was also detected. The genotype distribution in three populations of Ningxia from opposite sides of the Liupan Mountains revealed possible significant effects of the mountains on limiting gene flow and population exchange. Phylogenetic analysis confirmed the possibility of recombination in some of the studied subpopulations in both provinces.

scholarly journals Variable opportunities for outcrossing result in hotspots of novel genetic variation in a pathogen metapopulation

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Anna-Liisa Laine ◽  
Benoit Barrès ◽  
Elina Numminen ◽  
Jukka P Siren
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Structure ◽  
Fungal Pathogen ◽  
Population Genetic ◽  
Reproductive Strategies ◽  
Natural Populations ◽  
Pathogen Population ◽  
To Come ◽  
Pathogen Populations

Many pathogens possess the capacity for sex through outcrossing, despite being able to reproduce also asexually and/or via selfing. Given that sex is assumed to come at a cost, these mixed reproductive strategies typical of pathogens have remained puzzling. While the ecological and evolutionary benefits of outcrossing are theoretically well-supported, support for such benefits in pathogen populations are still scarce. Here, we analyze the epidemiology and genetic structure of natural populations of an obligate fungal pathogen, Podosphaera plantaginis. We find that the opportunities for outcrossing vary spatially. Populations supporting high levels of coinfection –a prerequisite of sex – result in hotspots of novel genetic diversity. Pathogen populations supporting coinfection also have a higher probability of surviving winter. Jointly our results show that outcrossing has direct epidemiological consequences as well as a major impact on pathogen population genetic diversity, thereby providing evidence of ecological and evolutionary benefits of outcrossing in pathogens.

scholarly journals Population Genetic Structure of Puccinia striiformis in Northwestern China

Plant Disease ◽  
2015 ◽  
Vol 99 (12) ◽  
pp. 1764-1774 ◽  
Author(s):  
Qiong Wan ◽  
Junmin Liang ◽  
Yong Luo ◽  
Zhanhong Ma
Keyword(s):  
Stripe Rust ◽  
Population Genetic ◽  
Fragment Length Polymorphism ◽  
Puccinia Striiformis ◽  
Genotypic Diversity ◽  
Gansu Province ◽  
Wheat Stripe Rust ◽  
Qinghai Province ◽  
Genetic Structures ◽  
Crop Planting

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important fungal diseases of wheat in China. Xinjiang, Qinghai, and Gansu Provinces, located in the northwest of China, are the key regions for interregional epidemics of wheat stripe rust due to their geographic as well as crop-planting features, in relation to pathogen’s life cycle, reproduction, and population genetics. To study the population genetic structures of the pathogen in these areas, 217 isolates of P. striiformis f. sp. tritici were collected from different geographic locations at various elevations in the three provinces. The amplified fragment length polymorphism (AFLP) genotypes and virulence phenotypes were analyzed for Xinjiang, Qinghai, and Gansu populations. Frequent genotypic exchanges between Xinjiang and Qinghai and between Qinghai and Gansu populations were detected, demonstrating that the populations of Xinjiang and Gansu may not be completely isolated. Genotypic diversity in Gansu Province was much higher than that in Xinjiang or Qinghai Province. The Xinjiang population was genetically closer to the Qinghai population than to the Gansu population. The race dynamic patterns in Qinghai were consistent with those in Gansu but the similar component pattern of the race dynamics in Xinjiang appeared late, following those in Gansu and other interior epidemic regions of China. No significant correlation between the AFLP genetic distance and the virulence distance in the Xinjiang, Qinghai, and Gansu populations was determined. A possible recombination signature of the pathogen population was detected in Gansu population and some subpopulations in Qinghai but not in Xinjiang population.

Identification of New Sources of Rust Resistance in Cowpea (Vigna Unguiculata L. Walp) Germplasm Using Simple Sequence Repeat Markers

2019 ◽  
Author(s):  
A. T. Asare ◽  
T. A. Mensah ◽  
S. M.A. Tagoe ◽  
D. K.A. Asante
Keyword(s):  
Fungal Pathogen ◽  
Simple Sequence Repeat ◽  
Rust Resistance ◽  
Gene Diversity ◽  
Yield Losses ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Breeding Programmes ◽  
Moderate Resistance ◽  
Simple Sequence

The demand for resistance cowpea to rust infection has currently increased due to considerable yield losses caused by the fungal pathogen. The study assessed available cowpea genotypes for rust resistance using simple sequence repeat (SSR) markers. Out of 100 cowpea genotypes screened, 97% showed presence of the markers whilst 3% showed absence of the markers. Among the cowpea were 72% resistance, 16% moderate resistance and 9% low resistance to rust. The markers revealed mean high allele frequency (0.86) and low gene diversity (0.24) and polymorphism information content (0.21) among the cowpea genotypes. The markers co-inherited with mean regression value greater than 0.1. There was no clear pattern of clustering among the cowpea genotypes. The cowpea genotypes with rust resistance traits could serve as good sources of germplasm for cultivation or resilient genes with rust target in breeding programmes to improve the crop.

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