scholarly journals Isolation and Identification of Chromium Reducing Bacillus Cereus Species from Chromium-Contaminated Soil for the Biological Detoxification of Chromium

2020 ◽  
Vol 17 (6) ◽  
pp. 2118 ◽  
Author(s):  
Ming-hao Li ◽  
Xue-yan Gao ◽  
Can Li ◽  
Chun-long Yang ◽  
Chang-ai Fu ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Immobilized Cells ◽  
Removal Rate ◽  
Human Beings ◽  
Isolation And Identification ◽  
High Chromium ◽  
Biological Detoxification ◽  
Chromium Tolerance ◽  
Chromium Detoxification ◽  
Temperature Adaptability

Chromium contamination has been an increasing threat to the environment and to human health. Cr(VI) and Cr(III) are the most common states of chromium. However, compared with Cr(III), Cr(VI) is more toxic and more easily absorbed, therefore, it is more harmful to human beings. Thus, the conversion of toxic Cr(VI) into Cr(III) is an accepted strategy for chromium detoxification. Here, we isolated two Bacillus cereus strains with a high chromium tolerance and reduction ability, named B. cereus D and 332, respectively. Both strains demonstrated a strong pH and temperature adaptability and survival under 8 mM Cr(VI). B. cereus D achieved 87.8% Cr(VI) removal in 24 h with an initial 2 mM Cr(VI). Cu(II) was found to increase the removal rate of Cr(VI) significantly. With the addition of 0.4 mM Cu(II), 99.9% of Cr(VI) in the culture was removed by B. cereus 332 in 24 h. This is the highest removal efficiency in the literature that we have seen to date. The immobilization experiments found that sodium alginate with diatomite was the better method for immobilization and B. cereus 332 was more efficient in immobilized cells. Our research provided valuable information and new, highly effective strains for the bioremediation of chromium pollution.

scholarly journals Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

2019 ◽  
Vol 60 (5) ◽  
pp. 985-995
Author(s):  
Yusur Ramzi ◽  
Hutaf A. A. Alsalim
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Mung Bean ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Hydrolytic Enzymes ◽  
Phosphate Solubilizing Bacteria ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and  of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 μg.ml-1), Y4 was the highest (39.00 μg.ml-1) followed by Y3 (37.00μg.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

scholarly journals Effective continuous acetone–butanol–ethanol production with full utilization of cassava by immobilized symbiotic TSH06

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhangnan Lin ◽  
Hongjuan Liu ◽  
Jing Wu ◽  
Petra Patakova ◽  
Barbora Branska ◽  
...  
Keyword(s):  
Large Scale ◽  
Immobilized Cells ◽  
Continuous Fermentation ◽  
Abe Fermentation ◽  
Human Beings ◽  
Butanol Production ◽  
System A ◽  
Glucose System ◽  
Acetone Butanol Ethanol ◽  
Full Utilization

Abstract Background Butanol production by fermentation has recently attracted increasingly more attention because of its mild reaction conditions and environmentally friendly properties. However, traditional feedstocks, such as corn, are food supplies for human beings and are expensive and not suitable for butanol production at a large scale. In this study, acetone, butanol, and ethanol (ABE) fermentation with non-pretreated cassava using a symbiotic TSH06 was investigated. Results In batch fermentation, the butanol concentration of 11.6 g/L was obtained with a productivity of 0.16 g/L/h, which was similar to that obtained from glucose system. A full utilization system of cassava was constructed to improve the fermentation performance, cassava flour was used as the substrate and cassava peel residue was used as the immobilization carrier. ABE fermentation with immobilized cells resulted in total ABE and butanol concentrations of 20 g/L and 13.3 g/L, which were 13.6% and 14.7% higher, respectively, than those of free cells. To further improve the solvent productivity, continuous fermentation was conducted with immobilized cells. In single-stage continuous fermentation, the concentrations of total ABE and butanol reached 9.3 g/L and 6.3 g/L with ABE and butanol productivities of 1.86 g/L/h and 1.26 g/L/h, respectively. In addition, both of the high product concentration and high solvent productivity were achieved in a three-stage continuous fermentation. The ABE productivity and concentration was 1.12 g/L/h and 16.8 g/L, respectively. Conclusions The results indicate that TSH06 could produce solvents from cassava effectively. This study shows that ABE fermentation with cassava as a substrate could be an efficient and economical method of butanol production.

Isolation and Identification of Bacillus from Spontaneous Fermented Sufu

2013 ◽  
Vol 634-638 ◽  
pp. 1179-1183 ◽  
Author(s):  
Jing Deng ◽  
Hua Chang Wu ◽  
Xing Xiu Zhao ◽  
Jiao Jiao Shi
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacillus Amyloliquefaciens ◽  
Fermented Food ◽  
Identification System ◽  
Isolation And Identification ◽  
Spontaneous Fermentation ◽  
Fermentation Products ◽  
Microbial Identification ◽  
Microbial Identification System

Sufu is a traditional Chinese fermented food. The safety of spontaneous fermentation products has been concerned by more and more people. A total of four isolates with big clear halos on the casein medium plate were isolated from spontaneous fermented Sufu in southern Sichuan. A1 and A3 most likely belong to Bacillus cereus B according to their phenotype and Biolog Microbial Identification System. B2 was classified in group as Bacillus amyloliquefaciens B with the same methods. A2 was identified as Bacillus subtilis according to their phenotype and 16SrRNA. The safety of the strains are also discussed.

scholarly journals Isolation and Identification of Bacillus cereus from Milk and Milk Products in Udaipur, Rajasthan, India

2019 ◽  
Vol 8 (09) ◽  
pp. 2783-2787
Author(s):  
Subhash Chand Meena ◽  
Abhishek Gaurav ◽  
S.S. Shekhawat ◽  
Bincy Joseph ◽  
Hitesh Kumar ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Isolation And Identification ◽  
Milk Products

scholarly journals Qualitative Detection and Isolation of Bacteria from Surfaces of Canned Drinks Sold in Ugbor, Benin City

2018 ◽  
Vol 3 (2) ◽  
pp. 20-25
Author(s):  
Ogofure G. Abraham ◽  
Bello-Osagie O. Idowu ◽  
Aduba U. Barbara ◽  
Ighodaro E. Veadams ◽  
Emoghene O. Alexander
Keyword(s):  
Bacillus Cereus ◽  
Bacterial Species ◽  
Qualitative Assessment ◽  
Public Health Importance ◽  
Isolation And Identification ◽  
Mannitol Salt Agar ◽  
Benin City ◽  
Diffusion Technique ◽  
Qualitative Detection ◽  
Mar Index

AbstractThe qualitative assessment of putative bacterial pathogens on the surfaces of canned drinks sold in Benin metropolis was evaluated in this study. Standard bacteriological culture-based techniques employing the use of selective and differential media (Oxoid) such asBacillus cereusagar, mannitol Salt agar,Pseudomonascetrimide agar, bile esculin agar and MacConkey agar were used for isolation and identification of bacteria from swabbed surfaces of canned drinks. Kirby-Bauer disc diffusion technique was used for antibacterial susceptibility testing. The multiple antibiotic resistance (MAR) index was deduced from the antibiogram characterization to evaluate the public health importance of the bacterial isolates. Refrigerated samples had 25% contamination while 75% were not contaminated and about 15.39% contamination was observed for non-refrigerated samples (stored in crates or cartons) compared to the counterpart 84.61%. The bacterial species includeStaphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, Bacillussp. andEnterococcussp. The bacteria were found to be sensitive to ciprofloxacin (92.5%) and gentamicin (90.1%) and least susceptible to cefixime (23.1%) and vancomycin (26.4%). They were found to be multi-resistant because they have an MAR index above the tolerable permissible limit (0.2) for common antibiotics usually used for their eradication. It is important to ensure that the surfaces of canned drinks must be rinsed with water before consumption.

scholarly journals Isolation and Identification of Bacillus cereus from Fish and their Handlers from Jammu, India

2017 ◽  
Vol 6 (8) ◽  
pp. 441-447
Author(s):  
U. Rasool ◽  
Ajaz Ahmad ◽  
G.A. Badroo ◽  
Mir Mudasir ◽  
S. Fayaz ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Isolation And Identification

Isolation and identification of low density polythene-degrading bacteria from soil of North West of Algeria

2019 ◽  
Vol 8 (3) ◽  
pp. 76-82
Author(s):  
K. Zerhouni ◽  
B. Abbouni ◽  
K. Kanoun ◽  
K. Larbi Daouadji ◽  
A. Tifrit ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Low Density Polyethylene ◽  
Low Density ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Daily Lives ◽  
North West ◽  
Degrading Bacteria ◽  
Solid Culture Medium ◽  
Chemical Cycling

Plas c bags (Low Density Polyethylene (LDPE) belong to the polymers, which plays a very important role in our daily lives by their diversi ed applica on. However, the accumula on of the plas c bags in the environment cons - tutes a serious problem and a real source for visual nuisance, pollu on of soil and marine environments. Furthermore, their biodegradation was the safest method of breakdown that possibly leaves behind less toxic residues and showed poten al of bio-geo chemical cycling of the substrate. The aim of the present work was the characterization of the isolated bacterial strains from a municipal land ll area of Tlemcen, North West Algeria, which were implicated by the biodegrada on ability of the Low Density Polyethylene. The degradation of the Low Density Polyethylene was inves gated by studying the bacterial growth of the isolated, inoculated on a solid culture medium, which was composed of LDPE as the sole carbon source with and with- out a nitrogen source and the selec on was based by the determination of the produced diameter of hydrolysis clear zone on the surface. Furthermore, the isolated, selected degrading Low Density Polyethylene bacterial ML002 has been iden ed by the study of their morphological, biochemical charac- teris cs and the ampli ca on of the fragment, coding the region of ARN 16S. The use of the API system indicated their belonging to the genus Bacillus Cereus, which has reduced the weight of LDPE by 0.26, 1.28, 1.53% a er 30, 90, 120 days respec vely. Furthermore, the amplified of the fragment, coding the region of ARN 16S by the isolated, selected bacterial ML002 indicated a similarity of 99.394% with Bacillus wiedmannii and Bacillus proteolyticus and 99.293% homology with Bacillus toyonensis, Bacillus cereus and Bacillus thuringiensis.

Effective Removal of Mercury Ions in Aqueous Solutions: A Review

2020 ◽  
Vol 16 (3) ◽  
pp. 363-375
Author(s):  
Kang Hua ◽  
Xueliu Xu ◽  
Zhiping Luo ◽  
Dong Fang ◽  
Rui Bao ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Ion Exchange ◽  
Biological Treatment ◽  
Metal Removal ◽  
Removal Rate ◽  
Synergistic Effects ◽  
Heavy Metal Removal ◽  
Future Research ◽  
Human Beings ◽  
Fast Removal

Background: Due to its high toxicity and bioaccumulation, the existence of mercury in the environment is always a big threat to human beings. In order to control mercury pollution, scientists have put great efforts in the past decades. Methods: Precipitation, adsorption, membrane separation, biological treatment and ion exchange are reviewed as a remover for mercury removal. For each material type, we not only reported on the removal mechanism, but also discussed the best areas for it. The correlation method and step-to-step focusing method have been used for references. Conclusion: With the exploration and application of research, people have mastered a variety of mature technologies for the treatment of mercury-containing wastewater. Using inexpensive adsorbents is a cost-effective method for treating low concentrations of heavy metal wastewater. Ion exchange with a fast removal rate has been widely used in the field of heavy metal removal from wastewater. The biological treatment method can effectively treat low-concentration mercurycontaining wastewater. However, there is still a need to develop novel mercury removers with high capacity, fast removal rate, and low removal limit. Nanomaterials with a high specific surface area on substrate with synergistic effects, such as high adsorption and ion exchange, are the future research points.

Isolation and Identification of a Cellulolytic Bacterium from the Huguang Maar Lake

2013 ◽  
Vol 706-708 ◽  
pp. 545-549 ◽  
Author(s):  
Li Hong Cui ◽  
Ji Hua Li ◽  
Xiao Yi Wei ◽  
Dong Hui Han ◽  
Yu Liang Zhang
Keyword(s):  
Phylogenetic Analysis ◽  
Bacillus Cereus ◽  
16S Rdna ◽  
Rdna Sequence ◽  
Cellulolytic Bacterium ◽  
Isolation And Identification ◽  
16S Rdna Sequence ◽  
Optimum Activity ◽  
Ph Tolerance ◽  
Maar Lake

In this study, a strain of cellulolytic bacterium named N-6 was isolated from lake Huguang Maar. Phylogenetic analysis based on a partial 16s rDNA sequence showed that the strain was Bacillus cereus. Extracellular cellulase produced by the strain Bacillus.cereus N-6 exhibits optimum activity around pH 8.0 at 50°C and has good pH tolerance in the range of 6-9.

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