scholarly journals Optimization of Biomethane Production via Fermentation of Chicken Manure Using Marine Sediment: A Modeling Approach Using Response Surface Methodology

Author(s):  
Fatma Abouelenien ◽  
Toyokazu Miura ◽  
Yutaka Nakashimada ◽  
Nooran S. Elleboudy ◽  
Mohammad S. Al-Harbi ◽  
...  

In this study, marine sediment (MS) was successfully used as a source of methanogenic bacteria for the anaerobic digestion (AD) of chicken manure (CM). Using MS showed high production in liquid and semi-solid conditions. Even in solid conditions, 169.3 mL/g volatile solids of chicken manure (VS-CM) was produced, despite the accumulation of ammonia (4.2 gNH3-N/kg CM). To the best of our knowledge, this is the highest methane production from CM alone, without pretreatment, in solid conditions (20%). Comparing MS to Ozouh sludge (excess activated sewage sludge) (OS), using OS under semi-solid conditions resulted in higher methane production, while using MS resulted in more ammonia tolerance (301 mL/gVS-CM at 8.58 gNH3-N/kg). Production optimization was carried out via a response surface methodology (RDM) model involving four independent variables (inoculum ratio, total solid content, NaCl concentration, and incubation time). Optimized methane production (324.36 mL/gVS-CM) was at a CM:MS ratio of 1:2.5 with no NaCl supplementation, 10% total solid content, and an incubation time of 45 days.

2017 ◽  
Vol 11 (1) ◽  
pp. 54-71 ◽  
Author(s):  
O. A. Aworanti ◽  
S. E. Agarry ◽  
O. O. Ogunleye

Background: The indiscriminate discharge of industrial waste, agricultural-biomass waste, waste of municipal, domestic and kitchen waste has negatively impacted on the environment and human health. It is very pertinent to reduce these impacts to the barest minimum through conversion of the waste to useful products. The conversion of these wastes to generate alternative energy to fossil fuel through the technology of anaerobic fermentation is one of the viable and more fascinating options for the management of waste. Objective: To investigate the interactive effect and optimization of process parameters of temperature, total solid content and feed/inoculum ratio on the biomethanization of the mixture of cattle manure, pig manure and poultry manure in co-digestion with waste peels of pineapple fruit and content of chicken-gizzard. Method: Full-factorial central composite design of experiment (RCCD) of the response surface method (RSM) was adopted to assess the possible interactive effects of the process variables and the optimal parameters (i.e. optimization) for biogas/biomethane production in an anaerobic digester. Result: The process variables had a significant (P < 0.05) positive and negative interactive effect on the biomethanization process. A second-order quadratic polynomial regression model which is statistically significant (p < 0.0001) was respectively obtained for cumulative biogas yield (CBY), biomethane content (BC) and hydraulic retention time (HRT). Temperature of 55.2°C; total solid content of 6.25%; and feed/inoculums ratio of 1:2 were found to be the optimum values required to attain a predicted optimum values of 6.261 dm3/g CBY, 71.54% BC within a minimum 8 days of HRT. At this optimum process conditions, the experimental observed maximum CBY, and BC with a minimum HRT were found to be 6.217 dm3/g of slurry, 71.10% and 7 days, respectively. Conclusion: Biogas/biomethane generation through co-substrate anaerobic fermentation of animal waste with waste of fruits constitute a reasonable and applicable renewable energy alternative and this can be optimized through response surface methodology.


Energy ◽  
2014 ◽  
Vol 74 ◽  
pp. 309-313 ◽  
Author(s):  
Yiqing Yao ◽  
Yang Luo ◽  
Yingxue Yang ◽  
Hongmei Sheng ◽  
Xiangkai Li ◽  
...  

1950 ◽  
Vol 28e (3) ◽  
pp. 113-120 ◽  
Author(s):  
J. R. Polley ◽  
M. M. Burr ◽  
A. L. Gillen

The pH, oxidation–reduction potential, total solids, nonspecific hemagglutination titer, and the titer of the inhibitor of influenza virus hemagglutination have been determined in the normal allantoic fluid of the chick embryo as the incubation time increased. These determinations were repeated after the allantoic fluid had been stored for 48 hr. at 0 °C. It was found that as the incubation time increased (1) the pH decreased progressively, (2) the Eh remained positive throughout, (3) the total solid content increased, (4) the nonspecific hemagglutination titer remained negligible, (5) the inhibition titer increased rapidly. Storage for 48 hr. at 0 °C. produced no change other than a small rise in the pH. By buffering the allantoic fluid in vivo, it appeared that the increasing inhibition titer was not directly related to the decreasing pH. The inhibition titer of the allantoic fluid was not decreased after dialysis, indicating that it is not affected by the amount of urates in the fluid. Thus it appears that the inhibition titer is related directly to a component of the increasing total solids which is unidentified as yet.


2014 ◽  
Vol 4 (02) ◽  
Author(s):  
Rina S. Soetopo ◽  
Sri Purwati ◽  
Henggar Hardiani ◽  
Mukharomah Nur Aini ◽  
Krisna Adhitya Wardhana

A continuous pilot scale study has been conducted to investigate the effectiveness of anaerobic digestion of biological sludge. The sludge has a total solid content of 0.53% - 1.1%, pH of 7.20 to 7.32. Its organic content is about 97 %, The research were conducted in two stages, which are acidification (performed in 3 m3 the Continously Stirred Tank Reactor/CSTR at pH of 5.5 to 6.0) and methanation (performed in 5 m3 the Up Flow Anaerobic Sludge Blanket/UASB reactor at pH 6.5 to 7.0). The retention time (RT) was gradually shortened from 6 days to 1 day for acidification and from 8 days to 2 days for methanation. The results showed that operating the CSTR at the RT of 1 day and the organic loading of 8.23 g Volatile Solid (VS)/m3.day could produce Volatile Fatty Acid (VFA) at an average value of 17.3 g/kg VS.day. Operating the UASB reactor at the RT of 2 days and the organic loading (Chemical Oxygen Demand/COD) of 2.4 kg COD/m3.day could produce biogas at an average value of 66.3 L/day, with an average methane content of 69.9%, methane rate of 0.17 L CH4/g COD reduction or 19.06 L CH4/kg VS. Furthermore, methanation could reduce COD at an average value of 51.2 %, resulting in the effluent average value of COD filtrate and COD total of 210.1 mg/L and 375.2 mg /L, respectively.Keywords: acidification, methanation, CSTR, UASB, biogas ABSTRAKPercobaan digestasi anaerobik lumpur IPAL biologi industri kertas secara kontinyu skala pilot telah dilakukan di industri kertas dengan tujuan mengkaji efektivitas proses digestasi anaerobik dalam mengolah lumpur tersebut. Lumpur yang digunakan memiliki total solids sekitar 0,53% – 1,1%, pH netral (7,20 – 7,32) dengan komponen utama senyawa organik sekitar 97%. Percobaan dilakukan dalam dua tahap yaitu asidifikasi dalam reaktor CSTR berkapasitas 3 m3 pada pH 5,5 – 6,0 dan metanasi dalam reaktor UASB berkapasitas 5 m3 pada pH 6,5 – 7,0. Percobaan dilakukan dengan waktu retensi yang dipersingkat secara bertahap dari 6 hari ke 1 hari untuk proses asidifikasi dan dari 8 hari ke 2 hari untuk proses metanasi. Hasil percobaan menunjukkan bahwa pengoperasian reaktor CSTR dengan waktu retensi 1 hari dan beban organik 8,3 g VS/m3.hari dapat menghasilkan VFA rata-rata 17,3 g/kg VS.hari dengan kisaran 8,36 – 30,59 g/kg VS.hari, sedangkan pengoperasian reaktor UASB pada waktu retensi 2 hari dan beban organik 2,4 kg COD/m3.hari dapat menghasilkan biogas rata-rata 66,3 L/hari dengan kadar metana rata-rata 69,9% atau 0,17 L CH4/g COD reduksi atau 19,06 L CH4/kg VS. Selain itu proses metanasi dapat menurunkan COD terlarut rata-rata 51,2%, dengan konsentrasi efluen COD terlarut  rata-rata 210,1 mg/L dan COD total rata-rata 375,2 mg/L.Kata kunci: asidifikasi, metanasi, CSTR, UASB, biogas


1995 ◽  
Vol 32 (12) ◽  
pp. 91-97 ◽  
Author(s):  
P. Y. Yang ◽  
M. Kuroshima

In order to develop a simple operation for an anaerobic treatment process for highly concentrated pig wastewater for small producers, a three-stage anaerobic treatment process was investigated. The system provided a series of mixing, homogenization, biological reaction and final stabilization of concentrated pig waste (total solid content of 8–10%). The process provided a stable operational performance, simple operational procedure and well stabilized sludge effluent. It was also found that the system is economically feasible in Hawaii. Compared to the other treatment processes for highly concentrated pig waste, this process is considered as an appropriate alternative for the application of the small producers in land limited and tropical conditions. Also, the present treatment system can be easily developed into a prefabricated package plant which can minimize the on-site labor and building costs.


2016 ◽  
Vol 19 (0) ◽  
Author(s):  
Ricardo Schmitz Ongaratto ◽  
Luiz Antonio Viotto

Summary The aim of this work was to separately evaluate the effects of pectinase and cellulase on the viscosity of pitanga juice, and determine the optimum conditions for their use employing response surface methodology. The independent variables were pectinase concentration (0-2.0 mg.g–1) and cellulase concentration (0-1.0 mg.g–1), activity time (10-110 min) and incubation temperature (23.2-56.8 °C). The use of pectinase and cellulase reduced the viscosity by about 15% and 25%, respectively. The results showed that enzyme concentration was the most important factor followed by activity time, and for the application of cellulase the incubation temperature had a significant effect too. The regression models showed correlation coefficients (R2) near to 0.90. The pectinase application conditions that led to the lowest viscosity were: concentration of 1.7 mg.g–1, incubation temperature of 37.6 °C and incubation time of 80 minutes, while for cellulase the values were: concentration of 1.0 mg.g-1, temperature range of 25 °C to 35 °C and incubation time of 110 minutes.


1960 ◽  
Vol 199 (4) ◽  
pp. 731-732 ◽  
Author(s):  
Jack R. Luick ◽  
Harold R. Parker ◽  
A. C. Andersen

A statistical study has been made of the composition of the major C-containing compounds taken from beagle dog milk during the 3rd through 5th week of lactation. The mean values are as follows: 26% total solids, 13% fat, 3.3% lactose and 9.8% protein. Total solid content was found to vary with fat content according to the equation: % total solids = 15.6 + 0.85 % fat.


2015 ◽  
Vol 1120-1121 ◽  
pp. 643-647
Author(s):  
Ya Bo Fu ◽  
Wen Cai Xu ◽  
Lu Fu ◽  
Dong Li Li ◽  
Jian Qing Wang

To reduce the loss of fresh fruits during transportation and storage, an active packaging (AP) material which could control release fungicide was prepared by melting, blowing film and composite method firstly. Then, its preservation effects on strawberry at different storage temperatures were investigated systematically. Several properties of preserved strawberries were measured periodically, including gas concentration of the package atmosphere and the physiological parameters contains decay rate, total solid content, as well as the sensory and taste evaluation were measured. The experimental results show that this AP material is helpful to fruit preservation, especially at lower temperature. It can prohibit the strawberry respiration, retard fungus growth and thus significantly extend the shelf-life of fresh strawberry.


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