scholarly journals Evaluation of the Toxicity of Bisphenol A in Reproduction and Its Effect on Fertility and Embryonic Development in the Zebrafish (Danio rerio)

2022 ◽  
Vol 19 (2) ◽  
pp. 962
Author(s):  
Lilian de Paula Gonçalves Reis ◽  
Antonio Jesús Lora-Benítez ◽  
Ana Mª Molina-López ◽  
Rafael Mora-Medina ◽  
Nahúm Ayala-Soldado ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Bisphenol A ◽  
Danio Rerio ◽  
Chemical Substance ◽  
Toxic Effects ◽  
Control Group ◽  
Histopathological Study ◽  
Parental Exposure ◽  
Electron Microscopes ◽  
Histopathological Studies

Bisphenol A (BPA) is a chemical substance commonly used in the manufacture of plastic products. Its inhalation or ingestion from particles in suspension, water, and/or polluted foods can trigger toxic effects related to endocrine disruption, resulting in hormonal, reproduction, and immunological alterations in humans and animals. The zebrafish (Danio rerio) is an ideal experimental model frequently used in toxicity studies. In order to assess the toxic effects of BPA on reproduction and embryonic development in one generation after parental exposure to it, a total of 80 zebrafish, males and females, divided into four groups in duplicate (n = 20) were exposed to BPA concentrations of 500, 50, and 5 µg L−1, along with a control group. The fish were kept in reproduction aquariums for 21 days. The embryos obtained in the crosses were incubated in a BPA-free medium and observed for signs of embryotoxicity. A histopathological study (under optical and electron microscopes) was performed of adult fish gonads. The embryos of reproducers exposed to BPA were those most frequently presenting signs of embryotoxicity, such as mortality and cardiac and musculoskeletal malformations. In the histopathological studies of adult individuals, alterations were found in ovocyte maturation and in spermatazoid formation in the groups exposed to the chemical. Those alterations were directly related to BPA action, affecting fertility in both sexes, as well as the viability of their offspring, proportionally to the BPA levels to which they were exposed, so that our results provide more information by associating toxic effects on the offspring and on the next generation.

Toxic effects of indoxacarb enantiomers on the embryonic development and induction of apoptosis in zebrafish larvae (Danio rerio)

2015 ◽  
Vol 32 (1) ◽  
pp. 7-16 ◽  
Author(s):  
Yongmei Fan ◽  
Qing Feng ◽  
Kehua Lai ◽  
Weikang Huang ◽  
Chenghui Zhang ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Danio Rerio ◽  
Toxic Effects ◽  
Zebrafish Larvae ◽  
Induction Of Apoptosis

Toxic effects of 56Fe ion radiation on the zebrafish (Danio rerio) embryonic development

2017 ◽  
Vol 186 ◽  
pp. 87-95 ◽  
Author(s):  
Jing Si ◽  
Rong Zhou ◽  
Jing’e Song ◽  
Lu Gan ◽  
Xin Zhou ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Danio Rerio ◽  
Toxic Effects

scholarly journals Effect of β-Cyclodextrin Stabilized Silver Nanoparticles on Gills, Kidney, Liver of Danio rerio

2021 ◽  
Vol 11 (1) ◽  
pp. 2981-2995
Keyword(s):  
Silver Nanoparticles ◽  
Danio Rerio ◽  
Morphological Changes ◽  
Antimicrobial Property ◽  
Research Work ◽  
Model Organism ◽  
Histopathological Study ◽  
Histopathological Studies ◽  
Kidney Liver ◽  
Drug Studies

The silver nanoparticles (AgNP) are applied broadly in medical applications due to their antimicrobial property. However, the toxicity (uptake, translocation, and accumulation) of these AgNPs nanomaterial has not been much explored. Also, cyclodextrin has been used for different pharmaceutical applications due to its various potential properties. Therefore, the toxicity of these AgNPs and cyclodextrin in the model organism such as Danio rerio could be crucial for future nano-drug studies. The main aim of the present research work is to synthesize, characterize biopolymeric silver nanoparticles, and perform histopathological studies of synthesized silver nanoparticles on Danio rerio. The silver nanoparticles of 130nm size have been synthesized successfully using β-Cyclodextrin (β-CD) at room temperature. The various analytical applied to characterized the β-Cyclodextrin (β-CD) reduced silver nanoparticles (β-CD)-SNPs. A histopathological study has been conducted to evaluate the toxicity effect of the synthesized (β-CD)-SNP on the animal model Danio rerio. The (β-CD)-SNPs conc. 30µl/l is affecting and damaged gills and kidney the Danio rerio organs (gills, kidney) exposed after 10 days, yet the liver was found to be healthy. In conclusion, the Danio rerio gills, kidneys, and liver are sensitive to the 130nm-sized (β-CD)-SNPs. The nanoparticles' toxicity depends on concentration; less concentration (30µl/l) accumulates and is absorbed efficiently than the higher concentration (300µl/l). However, no morphological changes were observed on Danio rerio.

scholarly journals Evaluation of the embryotoxicity in zebrafish (Danio rerio) of the flocculant and coagulant compounds used for water remediation

2019 ◽  
Vol 31 ◽  
Author(s):  
Diego Sousa Moura ◽  
Rafael Oliveira Duarte da Mota ◽  
José Francisco Gonçalves Júnior ◽  
Newton Flávio de Vasconcelos ◽  
Marco Aurélio Reis ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Danio Rerio ◽  
Sublethal Effects ◽  
Aquatic Toxicity ◽  
Zooplankton Biomass ◽  
Water Remediation ◽  
Fish Mortality ◽  
Control Group ◽  
Anionic Polymer ◽  
Aluminum Ions

Abstract Aim: Leaching from mining activities reaches water bodies, causing water contamination and acidification by toxic metals. As a consequence, oxygen depletion occurs, with the disappearance of certain benthic organisms, losses in phytoplankton and zooplankton biomass, and fish mortality. Remediation of contaminated water from mining involves the use of flocculants based on acrylamide monomers and polyacrylamide and of coagulants based on aluminum. Our aim was to assess the aquatic toxicity of the flocculant WF 424 and the coagulant WF 2000 PCA. Methods A flocculant anionic polymer (WF 424) and a coagulant polynuclear complex of polymerized hydro-aluminum ions (WF2000 PCA) were tested for embryotoxicity to zebrafish (Danio rerio, FET test - OEDC, 236, 2013). Results After 96 h of exposure to WF424, there was no significant lethality, nor were there sublethal effects such as hatching delay, heartbeat alteration, and malformation in embryonic development, when compared with the control group (p > 0.05). The coagulant compound WF2000 PCA did not show lethal effects during 96 h of exposure, and EC50 was considered as above the highest concentration-level tested. Conclusions For both compounds, no malformation, embryonic development delay or embryotoxicity were observed.

Individual and joint toxic effects of pentachlorophenol and bisphenol A on the development of zebrafish (Danio rerio) embryo

2008 ◽  
Vol 71 (3) ◽  
pp. 774-780 ◽  
Author(s):  
Zhenghua Duan ◽  
Lin Zhu ◽  
Lingyan Zhu ◽  
Yao Kun ◽  
Xiaoshan Zhu
Keyword(s):  
Bisphenol A ◽  
Danio Rerio ◽  
Toxic Effects

Screening of Toxic Effects of Bisphenol A and Products of Its Degradation: Zebrafish (Danio rerio) Embryo Test and Molecular Docking

Zebrafish ◽  
2016 ◽  
Vol 13 (5) ◽  
pp. 466-474 ◽  
Author(s):  
Katerina Makarova ◽  
Pawel Siudem ◽  
Katarzyna Zawada ◽  
Justyna Kurkowiak
Keyword(s):  
Molecular Docking ◽  
Bisphenol A ◽  
Danio Rerio ◽  
Toxic Effects

scholarly journals Chronic environmentally relevant levels of Simvastatin induces non-monotonic responses in Zebrafish (Danio rerio)

2018 ◽  
Author(s):  
Susana Barros ◽  
Rosa Montes ◽  
José Benito Quintana ◽  
Rosario Rodil ◽  
Ana André ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Danio Rerio ◽  
Mevalonate Pathway ◽  
Aquatic Organisms ◽  
Molecular Data ◽  
List Type ◽  
Parental Exposure ◽  
Multi Level ◽  
Dose Responses

AbstractSimvastatin (SIM), a hypocholesterolaemic compound, is among the most prescribed pharmaceuticals for cardiovascular disease prevention worldwide. Several studies have shown that acute exposure to SIM is able to produce multiple adverse effects in aquatic organisms. However, uncertainties still remain regarding the chronic effects of SIM in aquatic ecosystems. Therefore, the present study aimed to investigate the effects of SIM in the model freshwater teleost zebrafish (Danio rerio) following a chronic exposure (90 days) to environmentally relevant concentrations ranging from 8 ng/L to 1000 ng/L. This study used a multi-parametric approach integrating distinct ecological-relevant endpoints, i.e. survival, growth, reproduction and embryonic development, with biochemical markers (cholesterol and triglycerides). Furthermore, Real Time PCR was used to analyse the transcription levels of key genes involved in the mevalonate pathway (hmgcra, cyp51, and dhcr7). Globally, SIM induced several non-monotonic dose-responses; embryonic development, biochemical and molecular markers, were significantly impacted in the low-intermediate concentrations, 40 ng/L and 200 ng/L, whereas no effects were recorded for the highest tested SIM levels (1000 ng/L). Taken together, these findings expand our understanding of statins effects in teleost’s, demonstrating significant impacts at environmentally relevant concentrations. The findings highlight the importance of addressing the effects of chemicals under chronic low-level concentrations.Graphical abstractHighlightsSeveral uncertainties exist regarding simvastatin mode of action in non-target organismsThis work integrates D. rerio multi-level responses after long-term exposure to simvastatinSimvastatin impacted cholesterol/triglycerides levels and transcript levels of genes related to mevalonate pathway.Parental exposure to simvastatin induced offspring embryonic malformations.Embryonic abnormalities, biochemical and molecular data did follow a non-monotonic curve.

Disruption of zebrafish (Danio rerio) embryonic development after full life-cycle parental exposure to low levels of ethinylestradiol

2009 ◽  
Vol 95 (4) ◽  
pp. 330-338 ◽  
Author(s):  
J. Soares ◽  
A.M. Coimbra ◽  
M.A. Reis-Henriques ◽  
N.M. Monteiro ◽  
M.N. Vieira ◽  
...  
Keyword(s):  
Life Cycle ◽  
Embryonic Development ◽  
Danio Rerio ◽  
Parental Exposure ◽  
Full Life Cycle ◽  
Full Life ◽  
Low Levels

scholarly journals Assessment of the effects of bisphenol-A as a disruptor on ionic regulation in Danio rerio zebrafish through a study of their chloride and prolactin cells

2017 ◽  
Vol 58 (1) ◽  
pp. 105-116 ◽  
Author(s):  
Maria I. Barasona ◽  
Ana Molina ◽  
Alfonso Blanco ◽  
Nahum Ayala ◽  
Rosario Moyano
Keyword(s):  
Bisphenol A ◽  
Danio Rerio ◽  
Study Groups ◽  
Chloride Cells ◽  
Morphometric Study ◽  
Control Group ◽  
Histopathological Analysis ◽  
Ionic Exchange ◽  
Ionic Regulation ◽  
Prolactin Cells

Bisphenol A (BPA) is one of the chemicals produced in the largest volume in the world. It is commonly used as a component of plastics and food containers and can act as a xenoestrogen in humans. In view of the risk of exposure to it from the environment and diet, and basically as a water pollutant, the objective of our study was to assess possible effects on ionic regulation after exposure to BPA by means of a histopathological and morphometric study of the chloride and prolactin cells in zebrafish (Danio rerio) as an experimental model. Twenty-five male 16-week old zebrafish (Danio rerio) were allocated randomly into 5 study groups (n=5/group); a control group, and four groups, exposed for 2 weeks to a concentration of (1, 10, 100 and 1000 μg/L) of BPA, respectively. After 2 weeks, the animals were sacrificed and samples of their gills and pituitary gland were immediately taken for their subsequent histopathological analysis. Our results showed how, in the first study groups, lesions appeared in chloride cells, generating compensatory modifications in the prolactin cells, which were enough to maintain stability in the ionic exchange. As the exposure concentration increased, more serious histological modifications occurred. In the groups with the highest concentration (100 and 1000 μg/L), the lesions were so severe that the prolactin cells underwent some degenerative processes, which probably prevented the compensatory action at gill level.

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