Selected Morphological Characteristics, Lead Uptake and Phytochelatin Synthesis by Coffeeweed (Sesbania exaltata Raf.) Grown in Elevated Levels of Lead-Contaminated Soil

This study aimed to determine the effect of biochar and Trichoderma harzianum toward lead removal in soil, lead absorption, lead content in plant tissue also growth and yield of shallot cultivated on lead-contaminated soil. The experimental design used was a completely factorial randomized block design consisting of 2 factors. The first factor was corn cobs biochar which was applied 1 week after basic fertilizer treatment and consisted of 4 levels, namely B0: without biochar, B1: 2.5 t ha-1, B2: 5 t ha-1, and B3: 10 t ha-1. The second factor was the dosage of liquid of Trichoderma harzianum, namely TR0: without T.harzianum, TR1: 10 mL L-1, and TR2: 20 mL L-1, which was applied three times at 14, 28 and 42 days after planting. Data were analyzed using the F test and continued with DMRT (Duncan Multiple Range Test) at P= 0.05 level. The results showed that the application of 5 t biochar ha-1 was able to remove lead and decreased lead uptake in plants. Application of T. harzianum could remove and decrease absorption in plant tissue biochar was not able to increase the growth of shallot while T. harzianum increased the number of leaves and the number of tubers.

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Degradation of 2,3,7,8-TCDD by a consortium of bacterial strains isolated from heavil herbicide/dioxin contaminated soil in Bienhoa airbase

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/16/4/13275 ◽

2020 ◽

Vol 16 (4) ◽

pp. 777-784

Author(s):

Pham Quang Huy ◽

Nguyen Kim Thoa ◽

Dang Thi Cam Ha

Keyword(s):

Contaminated Soil ◽

Large Scale ◽

Morphological Characteristics ◽

Bacterial Consortium ◽

Soil Extract ◽

High Ratio ◽

Rrna Gene ◽

Bacterial Strains ◽

Agrobacterium Strain ◽

Microbial Strains

From two different soil sources in Bienhoa airbase (heavy herbicide/dioxin contaminated West-South region and bioremediated cell), five microbial strains were isolated and their 2,3,7,8-TCDD biodegrability in consortium was investigated. Based on the colony and cell morphological characteristics as well as 16S rRNA gene sequences, these strains were classified into 5 genera, including Methylobacterium (strain BHBi1), Hydrocarboniphaga (strain BHBi4), Agrobacterium (strain BHBi5), Bosea (strain BHBi7) and Microbacterium (strain BH09). Two strains BHBi7 and BHBi4 were the first representatives of the genera Bosea and Hydrocarboniphaga that were isolated from heavyly herbicide/dioxin contaminated soil. All five strains were able to grow well in mineral salt medium (MSM) supplemented with soil extract (SE) containing 2,3,7,8-TCDD (this congener is the main soil total compound toxicity) and other congeners, including PCDDs, PCDFs, 2,4,5-T, 2,4-D, PAHs and their intermediates. This microbial consortium degraded 2,537.34 ngTEQ/kg of 2,3,7,8-TCDD congener in soil, equivalent to 59.1% lost of total toxicity in comparison to the control without bacterial seeding (4,294.12 ng TEQ/kg). Such a high ratio of dioxin degradation by a bacterial consortium was reported here for the first time, contributing more evidences for convincing the successful dioxin bioremediation of “Active Landfill” technology at large scale in Z1 area at Bienhoa airbase, Dongnai, Vietnam.

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Combined application of Bacillus sp. MN-54 and phosphorus improved growth and reduced lead uptake by maize in the lead-contaminated soil

Environmental Science and Pollution Research ◽

10.1007/s11356-020-10372-4 ◽

2020 ◽

Vol 27 (35) ◽

pp. 44528-44539 ◽

Cited By ~ 1

Author(s):

Muhammad Junaid Afzal ◽

Muhammad Imran Khan ◽

Sardar Alam Cheema ◽

Saddam Hussain ◽

Muhammad Anwar-ul-Haq ◽

...

Keyword(s):

Contaminated Soil ◽

Bacillus Sp ◽

Lead Uptake ◽

Combined Application

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Lead uptake, tolerance, and accumulation exhibited by the plants Urtica dioica and Sedum spectabile in contaminated soil without additives

Archives of Biological Sciences ◽

10.2298/abs0802239g ◽

2008 ◽

Vol 60 (2) ◽

pp. 239-244 ◽

Cited By ~ 4

Author(s):

Milena Grubor

Keyword(s):

Contaminated Soil ◽

Total Content ◽

Urtica Dioica ◽

Atomic Spectroscopy ◽

Contaminated Sites ◽

Lead Uptake ◽

Chelating Compounds

Specimens of Urtica dioica and Sedum spectabile collected from plants growing at uncontaminated sites were transplanted in Pb-contaminated soil without additives (EDTA, HEDTA) to identify their natural potential for hyper-tolerance and hyperaccumulation of lead. The total content of Pb in the plants was determined by atomic spectroscopy. Our research showed that the concentrated toxic levels of lead (Pb) in Sedum spectabile and Urtica dioica were about 100 or more times higher than those of non-accumulator plants. It can be concluded that these plants have a high natural potential for hypertolerance and hyperaccumulation of lead, since they can hyperaccumulate it without addition of any chelating compounds (EDTA, HEDTA) to enhance lead uptake. This makes them very promising plants for use in phytoremediation of Pb-contaminated sites.

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Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065730 ◽

1971 ◽

Vol 29 ◽

pp. 338-339

Author(s):

M. J. Kramer ◽

Alan L. Coykendall

Keyword(s):

Cell Wall ◽

Dental Caries ◽

Streptococcus Mutans ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Genetic Heterogeneity ◽

Morphological Characteristics ◽

Electron Microscopic ◽

Dna Reassociation ◽

Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

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Aberrant Type C Virus Production in a Cell Line Derived from Balb/3T3

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094693 ◽

1972 ◽

Vol 30 ◽

pp. 286-287

Author(s):

N. Savage ◽

A. Hackett

Keyword(s):

Electron Microscopy ◽

Cell Line ◽

Leukemia Virus ◽

Morphological Characteristics ◽

Virus Production ◽

Oncogenic Viruses ◽

Endogenous Virus ◽

Virus Particles ◽

Moloney Leukemia Virus ◽

A Cell

A cell line, UC1-B, which was derived from Balb/3T3 cells, maintains the same morphological characteristics of the non-transformed parental culture, and shows no evidence of spontaneous virus production. Survey by electron microscopy shows that the cell line consists of spindle-shaped cells with no unusual features and no endogenous virus particles.UC1-B cells respond to Moloney leukemia virus (MLV) infection by a change in morphology and growth pattern which is typical of cells transformed by sarcoma virus. Electron microscopy shows that the cells are now variable in shape (rounded, rhomboid, and spindle), and each cell type has some microvilli. Virtually all (90%) of the cells show virus particles developing at the cell surface and within the cytoplasm. Maturing viruses, typical of the oncogenic viruses, are found along with atypical tubular forms in the same cell.

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DNA nick end labeling: a reliable marker for apoptosis?

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141184 ◽

1995 ◽

Vol 53 ◽

pp. 962-963

Author(s):

Anne F. Bushnell ◽

Sarah Webster ◽

Lynn S. Perlmutter

Keyword(s):

Cell Death ◽

Cultured Cells ◽

Apoptotic Cell ◽

Morphological Characteristics ◽

Detection Methods ◽

Tissue Preparation ◽

Preparation Methods ◽

Dna Laddering ◽

Disease States ◽

Reliable Marker

Apoptosis, or programmed cell death, is an important mechanism in development and in diverse disease states. The morphological characteristics of apoptosis were first identified using the electron microscope. Since then, DNA laddering on agarose gels was found to correlate well with apoptotic cell death in cultured cells of dissimilar origins. Recently numerous DNA nick end labeling methods have been developed in an attempt to visualize, at the light microscopic level, the apoptotic cells responsible for DNA laddering.The present studies were designed to compare various tissue processing techniques and staining methods to assess the occurrence of apoptosis in post mortem tissue from Alzheimer's diseased (AD) and control human brains by DNA nick end labeling methods. Three tissue preparation methods and two commercial DNA nick end labeling kits were evaluated: the Apoptag kit from Oncor and the Biotin-21 dUTP 3' end labeling kit from Clontech. The detection methods of the two kits differed in that the Oncor kit used digoxigenin dUTP and anti-digoxigenin-peroxidase and the Clontech used biotinylated dUTP and avidinperoxidase. Both used 3-3' diaminobenzidine (DAB) for final color development.

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