Objective: to study the composition of fatty acids (FA) and mitochondrial membrane potential (MMР) of blood cells in patients with chronic bronchitis (CB) and chronic obstructive pulmonary disease (COPD); to establish the role of the Mitochondrial dysfunction in the formation of respiratory diseases. Material and methods. The study involved 26 patients with chronic bronchitis, 27 - with mild COPD, 21 - with moderate COPD steady flow, 29 healthy people. MMР leukocytes was assessed by flow cytometry. The composition of the FA mitochondrial membranes was studied by gas-liquid chromatography. Results and discussion. With the worsening of the disease (moderate COPD) increases the number of white blood cells with reduced MMP, appears deficit of the saturated, monoenic (14: 0, 16: 0, 18: 0) and n-3 polyunsaturated fatty acids (18: 3n-3 20: 5n-3, 22: 5n-3, 22: 6n-3) in the membrane of the mitochondria. Identified imbalance in the composition of fatty acids verifies the changes in the physicochemical properties of subcellular membrane malfunction membrane transport systems, enzymes and receptors, processes of oxidative phosphorylation. Conclusion: The imbalance in the composition of the LCD mitochondrial membranes lead to the development of mitochondrial dysfunction and the formation of cell hypoxia.
Alterations in Serum Polyunsaturated Fatty Acids and Eicosanoids in Patients with Mild to Moderate Chronic Obstructive Pulmonary Disease (COPD)