scholarly journals Study on the Preparation and Chemical Structure Characterization of Melanin from Boletus griseus

2018 ◽  
Vol 19 (12) ◽  
pp. 3736 ◽  
Author(s):  
Qiuming Liu ◽  
Junjiang Xiao ◽  
Bingtong Liu ◽  
Yongliang Zhuang ◽  
Liping Sun
Keyword(s):  
Mass Spectrometry ◽  
Chemical Structure ◽  
High Resolution Mass Spectrometry ◽  
Ultra Performance Liquid Chromatography ◽  
Structure Characterization ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Pyrolysis Gas ◽  
Visible Absorption ◽  
Branched Chain

In this study, melanin (BgM) was obtained from Boletus griseus. The chemical composition and structure of BgM were characterized by UV-visible absorption spectrum, Fourier transform infrared spectrum, elemental analysis, nuclear magnetic resonance, pyrolysis gas chromatography mass spectrometry, and ultra-performance liquid chromatography–high resolution mass spectrometry. The percentage contents of C, H, N, S and O elements were 56.38%, 5.86%, 6.17%, 2.44%, and 28.04%, and the S/N and C/N ratios were 0.17 and 10.66, respectively. The UV-vis spectrum of BgM showed a maximum absorption peak at 214 nm. Characteristic absorption peaks were observed at 3426, 1600 and 1105 cm-1, and BgM contained phenolic hydroxyl, amidogen, carbonyl, methylene, and methyl groups. Moreover, BgM is an eumelanin, and its main skeleton has both a benzene ring and an indole, and the branched chain mainly consists of alkanes, alcohols, and fatty acids. BgM was hydrolyzed by H2O2 and four compounds were tentatively analyzed from the UPLC-MS/MS profile. The chemical structure of BgM was characterized as 5,6-dihydroxyindole eumelanin, and the condensed molecular formula is [C28(OR1)4(OR2)3H11O6N4]n.

scholarly journals First Evidence of “Earth Wax” Inside the Casting Molds from the Roman Era

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4259
Author(s):  
Klára Jagošová ◽  
Jan Jílek ◽  
Pavel Fojtík ◽  
Ivan Čižmář ◽  
Miroslav Popelka ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution Mass Spectrometry ◽  
Intermediate Stage ◽  
Gas Chromatography Mass Spectrometry ◽  
Organic Residue ◽  
Organic Residues ◽  
Pyrolysis Gas ◽  
Main Component ◽  
Solids Analysis ◽  
Ceramic Casting

This research was focused on the analysis of material composition and organic residues present in three molds found in the Moravian region (Czech Republic) belonging to the Roman era. X-ray fluorescence spectroscopy pointed out the possible remelting of Roman objects in Barbarian territory. The analysis of organic residues retrieved from the internal part of mold #2 by pyrolysis-gas chromatography/mass spectrometry proved the presence of ozokerite wax (“earth wax”). Consequent analysis of this organic residue by Atmospheric Solids Analysis Probe–ion mobility spectrometry–high-resolution mass spectrometry (ASAP-IMS-HRMS) confirmed the presence of ceresin, the main component of ozokerite. Ceresin was also detected in a sample of the organic residue from mold #1. Note that this is the first application of ASAP-IMS-HRMS in archaeological research. The remains of earth wax in molds suggest the production of wax models as an intermediate stage for the production of lost-wax ceramic casting molds.

A novel chemical marker of tremorgenic mycotoxicosis detected by gas-chromatography/mass-spectrometry

2021 ◽  
pp. 1-18
Author(s):  
C. Pickard ◽  
J.S. Fortin ◽  
D. Holmes ◽  
J.P. Buchweitz ◽  
A.F. Lehner
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Resolution Mass Spectrometry ◽  
Clinical Manifestations ◽  
Dietary Exposure ◽  
2D Nmr ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Mass Spectral ◽  
Chromatography Mass Spectrometry

Tremorgenic mycotoxicosis can arise from dietary exposure to secondary metabolite products of various fungal species, particularly those from the Penicillium genus. Although general toxin screens often rely on gas chromatography-mass spectrometry (GC/MS) and well-developed mass spectral library databases, two principal representative Penicillium mycotoxins, roquefortine and penitrem A, are unamenable to GC/MS owing to high molecular weights, low volatilities and/or high thermal instabilities. Reliance on GC/MS screens alone could therefore inadvertently result in failure to collect evidence of exposure to such tremorgenic mycotoxins. In this report we describe a newly discovered tremorgenic marker compound (TMC), the presence of which correlates highly with conclusive exposure to Penicillium toxins in explanation of clinical manifestations of tremorgenic mycotoxicosis. According to detailed mass spectral deconvolution, the compound is 210.0892 molecular weight, and amenable to GC/MS whether chemically underivatized or derivatized by methylation or trimethylsilylation. 1D and 2D NMR (nuclear magnetic resonance) studies on the isolated compound determined the TMC to be the Penicillium product terrestric acid, C11H14O4, which matches the molecular formula determined by high resolution mass spectrometry and thus provides an excellent target for assessment of mycotoxicosis by GC/MS.

CHARACTERISATION OF DISSOLVED ORGANIC CARBON (DOC) LEACHED FROM LEAVES IN WATER

2008 ◽  
Vol 6 (1) ◽  
Author(s):  
Markus Heryanto Langsa
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Total Organic Carbon ◽  
Gas Chromatography Mass Spectrometry ◽  
Pyrolysis Gas ◽  
Pyrolysis Gas Chromatography ◽  
Chromatography Mass Spectrometry ◽  
Pinus Radiate

<p>Penelitian ini bertujuan untuk menentukan senyawa organik khususnya organic karbon terlarut (DOC) dari dua spesies daun tumbuhan (<em>wandoo eucalyptus </em>and <em>pinus radiate, conifer</em>) yang larut dalam air selama periode 5 bulan leaching eksperimen. Kecepatan melarutnya senyawa organic ditentukan secara kuantitatif dan kualitatif menggunakan kombinasi dari beberapa teknik diantaranya Total Organic Carbon (TOC) analyser, Ultraviolet-Visible (UV-VIS) spektrokopi dan pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS).</p><p>Hasil analisis DOC dan UV menunjukkan peningkatan yang tajam dari kelarutan senyawa organic di awal periode pengamatan yang selanjutnya berkurang seiring dengan waktu secara eksponensial. Jumlah relatif senyawa organic yang terlarut tergantung pada luas permukaan, aktifitas mikrobiologi dan jenis sampel tumbuhan (segar atau kering) yang digunakan. Fluktuasi profil DOC dan UV<sub>254</sub> disebabkan oleh aktifitas mikrobiologi. Diperoleh bahwa daun kering lebih mudah terdegradasi menghasilkan senyawa organic dalam air dibandingkan dengan daun segar. Hasil pyrolysis secara umum menunjukkan bahwa senyawa hidrokarbon aromatic dan fenol (dan turunannya) lebih banyak ditemukan pada residue sampel setelah proses leaching kemungkinan karena adanya senyawa lignin atau aktifitas humifikasi mikrobiologi membuktikan bahwa senyawa-senyawa tersebut merupakan komponen penting dalam proses karakterisasi DOC.</p>

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