The Structure of Sucrose-Soaked Levansucrase Crystals from Erwinia tasmaniensis reveals a Binding Pocket for Levanbiose

Given its potential role in the synthesis of novel prebiotics and applications in the pharmaceutical industry, a strong interest has developed in the enzyme levansucrase (LSC, EC 2.4.1.10). LSC catalyzes both the hydrolysis of sucrose (or sucroselike substrates) and the transfructosylation of a wide range of acceptors. LSC from the Gram-negative bacterium Erwinia tasmaniensis (EtLSC) is an interesting biocatalyst due to its high-yield production of fructooligosaccharides (FOSs). In order to learn more about the process of chain elongation, we obtained the crystal structure of EtLSC in complex with levanbiose (LBS). LBS is an FOS intermediate formed during the synthesis of longer-chain FOSs and levan. Analysis of the LBS binding pocket revealed that its structure was conserved in several related species. The binding pocket discovered in this crystal structure is an ideal target for future mutagenesis studies in order to understand its biological relevance and to engineer LSCs into tailored products.

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Impact of culture condition modulation on the high-yield, high-specificity and cost-effective production of terpenoids from microbial sources: A review

Applied and Environmental Microbiology ◽

10.1128/aem.02369-20 ◽

2020 ◽

Author(s):

Vibha Shukla ◽

Suresh Chandra Phulara

Keyword(s):

Physicochemical Parameters ◽

High Specificity ◽

Cost Effective ◽

Fermentation Medium ◽

Culture Conditions ◽

High Yield ◽

Native Form ◽

Yield Production ◽

Wide Range ◽

The Impact

Recent years have seen a remarkable increase in the non-natural production of terpenoids from microbial route. This is due to the advancements in synthetic biology tools and techniques, which have overcome the challenges associated with the non-native production of terpenoids from microbial hosts. Although, microbes in their native form have ability to grow in wide range of physicochemical parameters such as, pH, temperature, agitation, aeration etc; however, after genetic modifications, culture conditions need to be optimized in order to achieve improved titers of desired terpenoids from engineered microbes. The physicochemical parameters together with medium supplements, such as, inducer, carbon and nitrogen source, and cofactor supply not only play an important role in high-yield production of target terpenoids from engineered host, but also reduce the accumulation of undesired metabolites in fermentation medium, thus facilitate product recovery. Further, for the economic production of terpenoids, the biomass derived sugars can be utilized together with the optimized culture conditions. In the present mini-review, we have highlighted the impact of culture conditions modulation on the high-yield and high-specificity production of terpenoids from engineered microbes. Lastly, utilization of economic feedstock has also been discussed for the cost-effective and sustainable production of terpenoids.

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Optimization of dilute acid-catalyzed hydrolysis of oil palm empty fruit bunch for high yield production of xylose

Chemical Engineering Journal ◽

10.1016/j.cej.2011.12.030 ◽

2012 ◽

Vol 181-182 ◽

pp. 636-642 ◽

Cited By ~ 48

Author(s):

Dongxu Zhang ◽

Yee Ling Ong ◽

Zhi Li ◽

Jin Chuan Wu

Keyword(s):

Oil Palm ◽

High Yield ◽

Empty Fruit Bunch ◽

Dilute Acid ◽

Yield Production ◽

Acid Catalyzed ◽

Hydrolysis Of ◽

High Yield Production

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High yield production of monomer-free chitosan oligosaccharides by pepsin catalyzed hydrolysis of a high deacetylation degree chitosan

Carbohydrate Research ◽

10.1016/j.carres.2007.08.023 ◽

2007 ◽

Vol 342 (18) ◽

pp. 2750-2756 ◽

Cited By ~ 51

Author(s):

Tomás Roncal ◽

Alberto Oviedo ◽

Iratxe López de Armentia ◽

Laura Fernández ◽

M. Carmen Villarán

Keyword(s):

High Yield ◽

Chitosan Oligosaccharides ◽

Deacetylation Degree ◽

Yield Production ◽

Hydrolysis Of ◽

High Yield Production

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High-yield production of mono- and di-oleylglycerol by lipase-catalyzed hydrolysis of triolein

Enzyme and Microbial Technology ◽

10.1016/0141-0229(96)00054-3 ◽

1996 ◽

Vol 18 (1) ◽

pp. 66-71 ◽

Cited By ~ 64

Author(s):

Francisco J. Plou ◽

María Barandiarán ◽

María V. Calvo ◽

Antonio Ballesteros ◽

Eitel Pastor

Keyword(s):

High Yield ◽

Yield Production ◽

Hydrolysis Of ◽

High Yield Production

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Ultrafast synthesis of isoquercitrin by enzymatic hydrolysis of rutin in a continuous-flow microreactor

Journal of the Serbian Chemical Society ◽

10.2298/jsc150914112w ◽

2015 ◽

Vol 80 (7) ◽

pp. 853-866 ◽

Cited By ~ 9

Author(s):

Jun Wang ◽

Gong An ◽

Shuangshuang Gu ◽

Hongsheng Cui ◽

Xiangyang Wu

Keyword(s):

Enzymatic Hydrolysis ◽

Continuous Flow ◽

Biological Activities ◽

Batch Reactor ◽

Maximum Yield ◽

Enzymatic Reaction ◽

High Yield ◽

Flow Microreactor ◽

Wide Range ◽

Hydrolysis Of

Isoquercitrin is a rare flavonol glycoside with a wide range of biological activities and is a key synthetic intermediate for the production of enzymatically modified isoquercitrin. In order to establish an ultrafast bioprocess for obtaining isoquercitrin, a novel continuous flow biosynthesis of isoquercitrin using the hesperidinase-catalyzed hydrolysis of rutin in a glass-polydimethylsiloxane (PDMS) microreactor was first carried out. Using the developed microchannel reactor (200?m width, 50?m depth, and 2 m length) with one T-shaped inlet and one outlet, the maximum yield of isoquercitrin (98.6%) was achieved in a short time (40 min) under the following optimum conditions: rutin concentration at 1 g L-1, hesperidinase concentration at 0.1 g mL-1, reaction temperature at 40?C, and a flow rate at 2 ?L min-1. The activation energy value Ea of the enzymatic reaction was 4.61 kJ mol-1, and the reaction rate and volumetric productivity were approximately 16.1-fold and 30% higher, respectively, than those in the batch reactor. Thus, the use of a continuous-flow microreactor for the enzymatic hydrolysis of rutin is an efficient and simple approach to achieve a relative high yield of isoquercitrin.

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Trace-Level Quantitation of Sulfonylurea Herbicides in Natural Water

Journal of AOAC International ◽

10.1093/jaoac/75.6.1084 ◽

1992 ◽

Vol 75 (6) ◽

pp. 1084-1090 ◽

Cited By ~ 4

Author(s):

Dean G Thompson ◽

Linda M Macdonald

Keyword(s):

Natural Water ◽

High Yield ◽

Chromatographic Behavior ◽

Electron Capture Detection ◽

Good Precision ◽

Sulfonylurea Herbicides ◽

Mass Spectral ◽

Hydrolysis Products ◽

Wide Range ◽

Hydrolysis Of

Abstract A novel gas chromatographic/electron capture detection (GC/ECD) technique is described for quantitation of trace sulfonylurea herbicide residues in natural water. In this method, the parent sulfonylurea herbicides are isolated from their aryl sulfonamide metabolites by Florisil chromatography, and the acidic aqueous hydrolysis products of the parent compounds are then quantitated. The method has been validated for both metsulfuron methyl (MET; methyl 2-{[(4-methoxy-6-methyl-1,3,5-triazin- 2-yl)aminocarbonyl] aminosulfonyl} benzoate), and chlorsulfuron [CHLOR; 2-chloro-A/-(4-methoxy-6- methyl-1,3,5-triazin-2-yl)-aminocarbonyl benzenesulfonamide]. Acidic hydrolysis of the isolated parent compounds MET and CHLOR produced a high yield of the corresponding aryl sulfonamides, methyl 2-(aminosulfonyl)benzoate and 2- chlorobenzenesulfonamide, as confirmed by mass spectral analysis. In-house validation studies confirmed excellent chromatographic behavior, high recovery efficiency (>80%), and generally good precision (<12% coefficient of variation) for quantitation of MET and CHLOR in fortified natural water samples over a wide range of concentrations (50 ppb, 500 ppt, and 50 ppt). Validated limits of quantitation were 50 ppt, with minimum limits of detection conservatively estimated as 10 ppt.

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Facile Synthesis of a Diverse Library of Mono-3-substituted β-Cyclodextrin Analogues

10.26434/chemrxiv.6453302 ◽

2018 ◽

Cited By ~ 1

Author(s):

Kathryn Kellett ◽

Brendan M. Duggan ◽

Michael Gilson

Keyword(s):

High Yield ◽

Facile Synthesis ◽

Wide Range

We have described simple, high-yield, protocols, which require only commonly accessible equipment, to synthesize a wide range of β-CD derivatives mono-substituted at the secondary face. These derivatives may be useful in their own right, and they are also scaffolds for further modification, and examples of the far broader array of derivatives that may be accessed by these procedures.

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