scholarly journals TMT-Based Quantitative Proteomic Analysis Reveals the Crucial Biological Pathways Involved in Self-Incompatibility Responses in Camellia oleifera

2020 ◽  
Vol 21 (6) ◽  
pp. 1987 ◽  
Author(s):  
Yifan He ◽  
Qianqian Song ◽  
Yuefeng Wu ◽  
Shutao Ye ◽  
Shipin Chen ◽  
...  
Keyword(s):  
Dynamic Change ◽  
Venn Diagram ◽  
Self Incompatibility ◽  
Camellia Oleifera ◽  
Tandem Mass Tag ◽  
Construction Of Self ◽  
Oil Plant ◽  
Self Compatibility ◽  
Adenosylmethionine Synthetase ◽  
Oil Source

Camellia oleifera is a valuable woody oil plant belonging to the Theaceae, Camellia oil extracted from the seed is an excellent edible oil source. Self-incompatibility (SI) in C. oleifera results in low fruit set, and our knowledge about the mechanism remains limited. In the present study, the Tandem mass tag (TMT) based quantitative proteomics was employed to analyze the dynamic change of proteins response to self- and cross-pollinated in C. oleifera. A total of 6,616 quantified proteins were detected, and differentially abundant proteins (DAPs) analysis identified a large number of proteins. Combined analysis of differentially expressed genes (DEGs) and DAPs of self- and cross-pollinated pistils based on transcriptome and proteome data revealed that several candidate genes or proteins involved in SI of C. oleifera, including polygalacturonase inhibitor, UDP-glycosyltransferase 92A1-like, beta-D-galactosidase, S-adenosylmethionine synthetase, xyloglucan endotransglucosylase/hydrolase, ABC transporter G family member 36-like, and flavonol synthase. Venn diagram analysis identified 11 proteins that may participate in pollen tube growth in C. oleifera. Our data also revealed that the abundance of proteins related to peroxisome was altered in responses to SI in C. oleifera. Moreover, the pathway of lipid metabolism-related, flavonoid biosynthesis and splicesome were reduced in self-pollinated pistils by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. In summary, the results of the present study lay the foundation for learning the regulatory mechanism underlying SI responses as well as provides valuable protein resources for the construction of self-compatibility C. oleifera through genetic engineering in the future.

scholarly journals A nonS-locus F-box gene breaks self-incompatibility in diploid potatoes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ling Ma ◽  
Chunzhi Zhang ◽  
Bo Zhang ◽  
Fei Tang ◽  
Futing Li ◽  
...  
Keyword(s):  
Potato Breeding ◽  
Inbred Lines ◽  
Hybrid Breeding ◽  
Self Incompatibility ◽  
Diploid Hybrid ◽  
Food Crop ◽  
Global Food Security ◽  
The Third ◽  
Self Compatibility ◽  
Global Food

AbstractPotato is the third most important staple food crop. To address challenges associated with global food security, a hybrid potato breeding system, aimed at converting potato from a tuber-propagated tetraploid crop into a seed-propagated diploid crop through crossing inbred lines, is under development. However, given that most diploid potatoes are self-incompatible, this represents a major obstacle which needs to be addressed in order to develop inbred lines. Here, we report on a self-compatible diploid potato, RH89-039-16 (RH), which can efficiently induce a mating transition from self-incompatibility to self-compatibility, when crossed to self-incompatible lines. We identify the S-locusinhibitor (Sli) gene in RH, capable of interacting with multiple allelic variants of the pistil-specific S-ribonucleases (S-RNases). Further, Sli gene functions like a general S-RNase inhibitor, to impart SC to RH and other self-incompatible potatoes. Discovery of Sli now offers a path forward for the diploid hybrid breeding program.

scholarly journals Downregulated expression of S2-RNase attenuates self-incompatibility in “Guiyou No. 1” pummelo

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jianbing Hu ◽  
Qiang Xu ◽  
Chenchen Liu ◽  
Binghao Liu ◽  
Chongling Deng ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Fruit Set ◽  
Spontaneous Mutation ◽  
Practical Significance ◽  
Spontaneous Mutant ◽  
Self Incompatibility ◽  
Yield And Quality ◽  
Citrus Maxima ◽  
Self Compatibility

AbstractSelf-incompatibility (SI) substantially restricts the yield and quality of citrus. Therefore, breeding and analyzing self-compatible germplasm is of great theoretical and practical significance for citrus. Here, we focus on the mechanism of a self-compatibility mutation in ‘Guiyou No. 1’ pummelo (Citrus maxima), which is a spontaneous mutant of ‘Shatian’ pummelo (Citrus maxima, self-incompatibility). The rate of fruit set and the growth of pollen tubes in the pistil confirmed that a spontaneous mutation in the pistil is responsible for the self-compatibility of ‘Guiyou No. 1’. Segregation ratios of the S genotype in F1 progeny, expression analysis, and western blotting validated that the reduced levels of S2-RNase mRNA contribute to the loss of SI in ‘Guiyou No. 1’. Furthermore, we report a phased assembly of the ‘Guiyou No. 1’ pummelo genome and obtained two complete and well-annotated S haplotypes. Coupled with an analysis of SV variations, methylation levels, and gene expression, we identified a candidate gene (CgHB40), that may influence the regulation of the S2-RNase promoter. Our data provide evidence that a mutation that affects the pistil led to the loss of SI in ‘Guiyou No. 1’ by influencing a poorly understood mechanism that affects transcriptional regulation. This work significantly advances our understanding of the genetic basis of the SI system in citrus and provides information on the regulation of S-RNase genes.

Floral Biology and Self-Incompatibility in Some Australian Mistletoes of the Genus Amyema (Loranthaceae)

1980 ◽  
Vol 28 (4) ◽  
pp. 437 ◽  
Author(s):  
P Bernhardt ◽  
RB Knox ◽  
DM Calder
Keyword(s):  
Floral Biology ◽  
Self Incompatibility ◽  
Flower Buds ◽  
In Vitro Pollination ◽  
Mature Flower ◽  
Pollen Presentation ◽  
Self Compatibility ◽  
Safe Mechanism ◽  
Fail Safe

Facultative cleistogamy has not been found In some Austrahan Amyema spp , and the stigma does not functlon in pollen presentation at anthesis. Wlthin mature flower buds self-polllnatlon is prevented by morphological and physiological barriers, a protandrous condition largely prevents mechanical or zoophilous self-pollination In vitro pollination experiments revealed that xenogamous crosses are most successful In the production of pollen tubes in the style although geitonogamy may act as a 'fail safe mechanism' at varying levels under natural condltlons in A miquelii (Lehm, ex Miq) Van Tiegh, A. miraculosum (Miq ) Van Tiegh and A. penduium (Sieber ex Spreng ) Van Tiegh. Of the four species studied, A. quandang (Lindl ) Van Tiegh had the highest levels of self-compatibility.

Genetic modifications of dimorphic incompatibility in the Turnera ulmifolia L. complex (Turneraceae)

1986 ◽  
Vol 28 (5) ◽  
pp. 796-807 ◽  
Author(s):  
J. S. Shore ◽  
S. C. H. Barrett
Keyword(s):  
Genetic Control ◽  
Moderate Degree ◽  
Self Incompatibility ◽  
Tetraploid Population ◽  
Polygenic Control ◽  
Genetic Modifications ◽  
Self Compatibility ◽  
Turnera Ulmifolia ◽  
Low Levels ◽  
Selection For

Diploid and tetraploid populations of Turnera ulmifolia are distylous and exhibit a strong self-incompatibility system. Distyly is governed by a single locus with two alleles. Several self-compatible variants were, however, obtained and the nature and genetic control of self-compatibility was assessed using controlled crosses. The study documented the occurrence of self-compatible variants in four contrasting situations. These included the following. (i) Self-compatibility in a diploid short-styled variant. The gene(s) governing self-compatibility interact with the distyly locus and are expressed only in short-styled plants. When tetraploids carrying the genes were synthesized, self-incompatibility reappeared. (ii) Self-compatibility occurred in a cross between geographically separate diploid populations. Self-compatibility appeared sporadically in the F1. Crosses revealed that self-compatibility is likely under polygenic control. (iii) Low levels of self-compatibility occurred in a tetraploid population. Crosses revealed that self-compatibility was under polygenic control. A small response to selection for increased self-compatibility was observed, (iv) Hexaploids were synthesized from crosses between distylous diploids and tetraploids. All hexaploids obtained were long- or short-styled indicating that hexaploidy per se does not cause homostyly. A single long-styled plant showed aberrant pollen behaviour, resulting in a moderate degree of self-compatibility. All of the variants studied exhibited either aberrant pollen or stylar incompatibility responses. In no instance was the genetic control of self-compatibility solely the result of segregation at the distyly locus.Key words: Turnera, dimorphic incompatibility, polyploidy, self-compatibility, distyly.

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