scholarly journals Ileal Transcriptome Profiles of Japanese Quail Divergent in Phosphorus Utilization

2020 ◽  
Vol 21 (8) ◽  
pp. 2762 ◽  
Author(s):  
Michael Oster ◽  
Henry Reyer ◽  
Nares Trakooljul ◽  
Frank M. Weber ◽  
Lu Xi ◽  
...  
Keyword(s):  
Japanese Quail ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Animal Husbandry ◽  
Coturnix Japonica ◽  
P Efficiency ◽  
Mitochondrial Energy Metabolism ◽  
False Discovery ◽  
P Utilization ◽  
Total P

Phosphorus (P) is an essential component for all living beings. Low P diets prompt phenotypic and molecular adaptations to maintain P homeostasis and increase P utilization (PU). Knowledge of the molecular mechanisms of PU is needed to enable targeted approaches to improve PU efficiency and thus lower P excretion in animal husbandry. In a previous population study, Japanese quail were subjected to a low P diet lacking mineral P and exogenous phytase. Individual PU was determined based on total P intake and excretion. A subset of 20 extreme siblings discordant for PU was selected to retrieve gene expression patterns of ileum (n = 10 per PU group). Sequencing reads have been successfully mapped to the current Coturnix japonica reference genome with an average mapping rate of 86%. In total, 640 genes were found to be differentially abundant between the low and high PU groups (false discovery rate ≤ 0.05). Transcriptional patterns suggest a link between improved PU and mitochondrial energy metabolism, accelerated cell proliferation of enterocytes, and gut integrity. In assessing indicators of the efficient use of macro- and micronutrients, further research on turnover and proliferation rates of intestinal cells could provide an approach to improve P efficiency in poultry species.

scholarly journals Expression profiling of sexually dimorphic genes in the Japanese quail, Coturnix japonica

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Miki Okuno ◽  
Shuntaro Miyamoto ◽  
Takehiko Itoh ◽  
Masahide Seki ◽  
Yutaka Suzuki ◽  
...  
Keyword(s):  
Japanese Quail ◽  
Expression Profiling ◽  
Molecular Mechanisms ◽  
Sex Differentiation ◽  
Expression Patterns ◽  
Coturnix Japonica ◽  
Initial Increase ◽  
Sexually Dimorphic ◽  
Developmental Processes ◽  
Key Genes

AbstractResearch on avian sex determination has focused on the chicken. In this study, we established the utility of another widely used animal model, the Japanese quail (Coturnix japonica), for clarifying the molecular mechanisms underlying gonadal sex differentiation. In particular, we performed comprehensive gene expression profiling of embryonic gonads at three stages (HH27, HH31 and HH38) by mRNA-seq. We classified the expression patterns of 4,815 genes into nine clusters according to the extent of change between stages. Cluster 2 (characterized by an initial increase and steady levels thereafter), including 495 and 310 genes expressed in males and females, respectively, contained five key genes involved in gonadal sex differentiation. A GO analysis showed that genes in this cluster are related to developmental processes including reproductive structure development and developmental processes involved in reproduction were significant, suggesting that expression profiling is an effective approach to identify novel candidate genes. Based on RNA-seq data and in situ hybridization, the expression patterns and localization of most key genes for gonadal sex differentiation corresponded well to those of the chicken. Our results support the effectiveness of the Japanese quail as a model for studies gonadal sex differentiation in birds.

scholarly journals KORELASI GENETIK DAN FENOTIPIK BOBOT BADAN UMUR EMPAT MINGGU DENGAN BOBOT TELUR PADA PUYUH JEPANG (Coturnix coturnix japonica) Genotipic and Fenotipic Correlation Among Four-Week Body Weight and Eggs Weight Of Japanese Quail (Coturnix coturnix japonica)

2018 ◽  
Vol 1 (2) ◽  
pp. 15
Author(s):  
Dini Widianingrum
Keyword(s):  
Body Weight ◽  
Key Words ◽  
Japanese Quail ◽  
Animal Husbandry ◽  
Coturnix Japonica ◽  
Coturnix Coturnix Japonica ◽  
Coturnix Coturnix ◽  
High Category ◽  
Selection Of ◽  
Estimated Heritability

Abstrak Penelitian mengenai korelasi genetik dan fenotipik bobot badan umur empat minggu dengan bobot telur telah dilaksanakan di Program Studi Peternakan Fakultas Pertanian Universitas Majalengka. Tujuan penelitian yaitu untuk mempelajari korelasi genetik dan fenotipik bobot badan umur empat minggu dengan bobot telur. Percobaan dilakukan pada 369 ekor puyuh betina yang merupakan keturunan pertama dari pejantan 60 ekor dan betina 120 ekor yang ditetaskan dalam 3 periode penetasan. Metode penelitian menggunakan hubungan saudara tiri sebapak (paternal halfsib correlatian) dengan pola (nested unequal subclass number). Parameter genetik yang diamati yaitu heritabilitas, korelasi genetik dan korelasi fenotipik bobot badan umur empat minggu bobot telur. Hasil penelitian menunjukan bahwa nilai heritabilitas bobot badan umur empat minggu dan bobot telur yaitu 0,563+0,226 dan 0,584+0,281 termasuk kategori tinggi. Nilai korelasi genetik dan fenotipik bobot badan umur empat minggu dengan bobot telur yaitu 0,824+0,187 dan 0,693+0,245. Hal demikian menunjukan bahwa seleksi untuk bobot telur dapat dilakukan pada bobot badan umur empat minggu Kata kunci : heritabilitas, korelasi genetik dan fenotipik, bobot badan dan bobot telur puyuh Abstract The research was conducted at animal husbandry studi program faculty of agricultural Majalengka University. The object of research were study about four-week body weight and eggs weight heritability’s, and than genetic and fenotipic correlation between four-week body weight and eggs weight. Material used were 369 female japanese quail from 60 sire and 120 dam from third periode of hacthery. The method which applied was experimental. The data were analyzed statistically using unequal subclass number design. The result of research estimated heritability obtained using faternal halfsib correlation were four-week body weight 0,563+0,226 was high category and eggs weight 0,584+0,281was high category. Genetic and fenotipic correlation between four-week body weight and eggs weight 0,824+0,187 dan 0,693+0,245. This result shows that selection of one trait will give advantage respon to another trait. Key words : heritability, genetic and fenotipic correlation, body weight dan eggs weight of quail

scholarly journals Mitochondrial function, fatty acid metabolism, and immune system are relevant features of pig adipose tissue development

2012 ◽  
Vol 44 (22) ◽  
pp. 1116-1124 ◽  
Author(s):  
Annie Vincent ◽  
Isabelle Louveau ◽  
Florence Gondret ◽  
Bénédicte Lebret ◽  
Marie Damon
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Enrichment Analysis ◽  
Functional Enrichment Analysis ◽  
Functional Enrichment ◽  
Low Grade ◽  
Large White ◽  
Mitochondrial Energy Metabolism

The molecular mechanisms underlying the genetic control of fat development in humans and livestock species still require characterization. To gain insights on gene expression patterns associated with genetic propensity for adiposity, we compared subcutaneous adipose tissue (SCAT) transcriptomics profiles from two contrasted pig breeds for body fatness. Samples were obtained from Large White (LW; lean phenotype) and Basque pigs (B; low growth and high fat content) at 35 kg ( n = 5 per breed) or 145 kg body weight ( n = 10 per breed). Using a custom adipose tissue microarray, we found 271 genes to be differentially expressed between the two breeds at both stages, out of which 123 were highly expressed in LW pigs and 148 genes were highly expressed in B pigs. Functional enrichment analysis based on gene ontology (GO) terms highlighted gene groups corresponding to the mitochondrial energy metabolism in LW pigs, whereas immune response was found significantly enriched in B pigs. Genes associated with lipid metabolism, such as ELOVL6, a gene involved in fatty acid elongation, had a lower expression in B compared with LW pigs. Furthermore, despite enlarged adipocyte diameters and higher plasma leptin concentration, B pigs displayed reduced lipogenic enzyme activities compared with LW pigs at 145 kg. Altogether, our results suggest that the development of adiposity was associated with a progressive worsening of the metabolic status, leading to a low-grade inflammatory state, and may thus be of significant interest for both livestock production and human health.

Japanese Quail (Coturnix japonica)

2020 ◽  
Author(s):  
Philip J.K. McGowan ◽  
Guy M. Kirwan
Keyword(s):  
Japanese Quail ◽  
Coturnix Japonica

scholarly journals Circular RNA AFF4 modulates osteogenic differentiation in BM-MSCs by activating SMAD1/5 pathway through miR-135a-5p/FNDC5/Irisin axis

2021 ◽  
Vol 12 (7) ◽  
Author(s):  
Chao Liu ◽  
An-Song Liu ◽  
Da Zhong ◽  
Cheng-Gong Wang ◽  
Mi Yu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Regulatory System ◽  
Circular Rna ◽  
Luciferase Reporter ◽  
Integrin Αv

AbstractBone marrow-derived mesenchymal stem cells (BM-MSCs), the common progenitor cells of adipocytes and osteoblasts, have been recognized as the key mediator during bone formation. Herein, our study aim to investigate molecular mechanisms underlying circular RNA (circRNA) AFF4 (circ_AFF4)-regulated BM-MSCs osteogenesis. BM-MSCs were characterized by FACS, ARS, and ALP staining. Expression patterns of circ_AFF4, miR-135a-5p, FNDC5/Irisin, SMAD1/5, and osteogenesis markers, including ALP, BMP4, RUNX2, Spp1, and Colla1 were detected by qRT-PCR, western blot, or immunofluorescence staining, respectively. Interactions between circ_AFF4 and miR-135a-5p, FNDC5, and miR-135a-5p were analyzed using web tools including TargetScan, miRanda, and miRDB, and further confirmed by luciferase reporter assay and RNA pull-down. Complex formation between Irisin and Integrin αV was verified by Co-immunoprecipitation. To further verify the functional role of circ_AFF4 in vivo during bone formation, we conducted animal experiments harboring circ_AFF4 knockdown, and born samples were evaluated by immunohistochemistry, hematoxylin and eosin, and Masson staining. Circ_AFF4 was upregulated upon osteogenic differentiation induction in BM-MSCs, and miR-135a-5p expression declined as differentiation proceeds. Circ_AFF4 knockdown significantly inhibited osteogenesis potential in BM-MSCs. Circ_AFF4 stimulated FNDC5/Irisin expression through complementary binding to its downstream target molecule miR-135a-5p. Irisin formed an intermolecular complex with Integrin αV and activated the SMAD1/5 pathway during osteogenic differentiation. Our work revealed that circ_AFF4, acting as a sponge of miR-135a-5p, triggers the promotion of FNDC5/Irisin via activating the SMAD1/5 pathway to induce osteogenic differentiation in BM-MSCs. These findings gained a deeper insight into the circRNA-miRNA regulatory system in the bone marrow microenvironment and may improve our understanding of bone formation-related diseases at physiological and pathological levels.

scholarly journals Fos expression in the medial preoptic area and nucleus accumbens of female Japanese quail (Coturnix japonica) after maternal induction and interaction with chicks

2021 ◽  
Vol 234 ◽  
pp. 113357
Author(s):  
Joseph S. Lonstein ◽  
Thierry D. Charlier ◽  
Jodi L. Pawluski ◽  
Nadege Aigueperse ◽  
Maryse Meurisse ◽  
...  
Keyword(s):  
Nucleus Accumbens ◽  
Japanese Quail ◽  
Preoptic Area ◽  
Medial Preoptic Area ◽  
Coturnix Japonica ◽  
Fos Expression
Sign in / Sign up

Export Citation Format

Share Document