Characterization and Analysis of the Temporal and Spatial Dynamic of Several Enteritis Modeling Methodologies

Inflammatory bowel disease (IBD), such as Crohn’s disease and ulcerative colitis, is a complex disease involving genetic, immune, and microbiological factors. A variety of animal models of IBD have been developed to study the pathogenesis of human IBD, but there is no model that can fully represent the complexity of IBD. In this study, we established two acute enteritis models by oral 3% DSS or intraperitoneal injection of anti-CD3 antibody, and two chronic enteritis models by feeding 3 cycles of 1.5% DSS or 3 months of the high-fat diet, respectively, and then examined the clinical parameters, histological changes, and cytokine expression profiles after the successful establishment of the models. Our results indicated that in 3% DSS-induced acute enteritis, the colorectal injury was significantly higher than that of the small intestine, while in anti-CD3 antibody-induced acute enteritis, the small intestine injury was significantly higher than that of colorectal damage. Besides, in the 1.5% DSS-induced chronic enteritis, the damage was mainly concentrated in the colorectal, while the damage caused by long-term HFD-induced chronic enteritis was more focused on the small intestine. Therefore, our work provides a reference for selecting appropriate models when conducting research on factors related to the pathogenesis of IBD or evaluating the potential diagnosis and treatment possibilities of pharmaceuticals.

Complications of Chickenpox in Bulgaria – Data from a Single-Center Experience

Primary Varicella zoster infection (chickenpox) in childhood is a common benign disease. The predominance of uncomplicated cases in children often overshadows the rarer cases of complicated severe course of the disease. Objective To study the complications of primary chickenpox in hospitalized patients for a period of one year. Materials and Methods 122 patients with complicated primary chickenpox with an average age of 18.6 years; 57 males (46.7%) and 64 females (53.3%), were hospitalized at the University Hospital of Infectious and Parasitic Diseases “Prof. Iv. Kirov” in the period January – December 2019. Results The bacterial infections of the skin and soft tissues comprised the largest relative frequenchy – they occurred in 74 (60.6%) of the patients; followed by pneumonia – in 31 (25.4%) patients, and a combination of pneumonia and pustulation – in 10 (8.2%) patients. Cerebelitis was observed in 1 (0.8%) patient. One of the patients developed hepatitis, purulent arthritis and acute enteritis. We also report the death of one patient. Conclusions We registered a relatively high proportion of hospitalizations, especially in young children and adults. Regarding the type of complications, we do not describe significant differences compared to other authors. Although in most cases primary chickenpox is a self-limiting disease, complications are more common in the countries where there is no vaccine.

Application of chitosan as a natural disinfectant against porcine epidemic diarrhoea virus

Porcine epidemic diarrhoea virus (PEDV) is one of the major pathogens causing acute enteritis, which is characterised by vomiting and watery diarrhoea and commonly leads to high rates of mortality and morbidity in suckling piglets. Chitosan has been regarded as a promising natural disinfectant. In this study, the disinfectant effect and mammalian-cell toxicity of chitosan were evaluated against PEDV using Vero cells. A 0.01% solution of chitosan was determined to be an effective disinfectant. In addition, no evidence of toxicity was observed during the cell toxicity test; on the contrary, chitosan promoted cell proliferation. In conclusion, chitosan is a promising candidate for an effective and safe disinfectant against PEDV as well as other coronaviruses.

Risk factors associated with canine parvovirus disease in dogs: A case-control study

Canine parvovirus type-2 (CPV-2) is one of the most contagious viral agents causing acute enteritis in pups with high rate of morbidity and mortality. The study aimed to investigate the risk factors associated with canine parvovirus (CPV) occurrence in dogs in Ho Chi Minh City, Vietnam. A total number of 132 dogs less than six months of age were recruited in the study and split into two groups: ‘CPV diseased dogs’ included 44 (33.3%) dogs positive with canine parvovirus confirmed by rapid immune-migration test, and ‘normal dogs’ consisted of 88 (66.7%) healthy dogs. Results indicated that the unvaccinated dogs had 11.76 times more risk of CPV infection than vaccinated dogs (OR = 11.76,P<0.001). The risk of CPV in dogs raised with others at the same age was 5.01 times (OR = 5.01,P= 0.027) higher than those raised singly. The risk of CPV infection of dogs interacting with neighbour dogs was 3.13 times (OR = 3.13,P= 0.028) higher than the dogs having no contact with other dogs living nearby. Briefly, our study highlights potential factors for CPV disease prevention, especially CPV vaccination is the most important driver affecting the occurrence of parvovirus in young dogs

Aliarcobacter butzleri from Water Poultry: Insights into Antimicrobial Resistance, Virulence and Heavy Metal Resistance

Aliarcobacter butzleri is the most prevalent Aliarcobacter species and has been isolated from a wide variety of sources. This species is an emerging foodborne and zoonotic pathogen because the bacteria can be transmitted by contaminated food or water and can cause acute enteritis in humans. Currently, there is no database to identify antimicrobial/heavy metal resistance and virulence-associated genes specific for A. butzleri. The aim of this study was to investigate the antimicrobial susceptibility and resistance profile of two A. butzleri isolates from Muscovy ducks (Cairina moschata) reared on a water poultry farm in Thuringia, Germany, and to create a database to fill this capability gap. The taxonomic classification revealed that the isolates belong to the Aliarcobacter gen. nov. as A. butzleri comb. nov. The antibiotic susceptibility was determined using the gradient strip method. While one of the isolates was resistant to five antibiotics, the other isolate was resistant to only two antibiotics. The presence of antimicrobial/heavy metal resistance genes and virulence determinants was determined using two custom-made databases. The custom-made databases identified a large repertoire of potential resistance and virulence-associated genes. This study provides the first resistance and virulence determinants database for A. butzleri.

Antimicrobial Susceptibility of Campylobacter isolates in the Capital of North Macedonia

Background: Campylobacter infections are typically self-limited, but in cases with severe enteritis, immuno-compromised system and bacteremia, an appropriate antimicrobial treatment is demanding. Our study aim was to determine the isolation rate of Campylobacter among patients with acute enteritis in the capital of North Macedonia and its antimicrobial susceptibility. Material and methods: A total number of 3820 patients clinically diagnosed as acute enteritis, were included in the study. Stool samples were collected and Campylobacter was isolated and identified by classical microbiological methods. Antimicrobial susceptibility of all isolates to Ceftriaxone, Amoxicillin-clavulonic acid, Erythromycin, Ciprofloxacin, Tetracycline and Gentamicin was determined by disc-diffusion technique. Additionally, minimal inhibitory concentrations of all Campylobacter isolates against erythromycin, ciprofloxacin and tetracycline were determined by Epsilon gradient tests. Results: Campylobacter species was isolated in 97 patients. Although the mean isolation rate of Campylobacter spp. during the whole study period was 2.53%, a statistically significant increase was detected in 2016 and 2017, in comparison with the data from previous four years of the study. The isolation rate of Campylobacter spp. didn’t reveal statistically significant difference between males and females (p > 0.05). 46.4 % of patients with Campylobacter enteritis were children at the age under 15 years. Forty-three C. jejuni isolates were susceptible to all six antibiotics, but the remaining 44 isolates revealed resistance to at least one antibiotic. C. coli isolates were resistant to 3 antibiotics simultaneously. Two C. coli isolates only, were susceptible to all 6 antibiotics. 40.90% of C. jejuni and 50% of C. coli isolates were resistant to beta-lactams, fluoroquinolones and tetracyclines, simultaneously. Conclusion: The increase of the isolation rate of Campylobacter from patients with acute enteritis indicates the need for permanent isolation and identification of Campylobacter from every clinically diagnosed patient, as acute enteritis. Erythromicin is the most effective antibiotic for treatment of Campylobacter enteritis in our patients. The high level of Campylobacter resistance to beta-lactams, fluoroquinolones and tetracyclines requires more rational approach in the treatment of Campylobacter enteritis