scholarly journals All d-Lysine Analogues of the Antimicrobial Peptide HPA3NT3-A2 Increased Serum Stability and without Drug Resistance

2020 ◽  
Vol 21 (16) ◽  
pp. 5632
Author(s):  
Jong-Kook Lee ◽  
Yoonkyung Park
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Peptide ◽  
Proteolytic Enzymes ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Peptide Bonds ◽  
Drug Resistant Bacteria ◽  
Antibiotic Drugs ◽  
Strong Antimicrobial Activity

Novel antibiotic drugs are urgently needed because of the increase in drug-resistant bacteria. The use of antimicrobial peptides has been suggested to replace antibiotics as they have strong antimicrobial activity and can be extracted from living organisms such as insects, marine organisms, and mammals. HPA3NT3-A2 ([Ala1,8] HPA3NT3) is an antimicrobial peptide that is an analogue of the HP (2–20) peptide derived from Helicobacter pylori ribosomal protein L1. Although this peptide was shown to have strong antimicrobial activity against drug-resistant bacteria, it also showed lower toxicity against sheep red blood cells (RBCs) and HaCaT cells compared to HPA3NT3. The l-Lys residues of HPA3NT3-A2 was substituted with d-Lys residues (HPA3NT3-A2D; [d-Lys2,5,6,9,10,15] HPA3NT3-A2) to prevent the cleavage of peptide bonds by proteolytic enzymes under physiological conditions. This peptide showed an increased half-life and maintained its antimicrobial activity in the serum against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) (pathogen). Furthermore, the antimicrobial activity of HPA3NT3-A2D was not significantly affected in the presence of mono- or divalent ions (Na+, Mg2+, Ca2+). Finally, l- or d-HPA3NT3-A2 peptides exhibited the strongest antimicrobial activity against antibiotic-resistant bacteria and failed to induce resistance in Staphylococcus aureus after 12 passages.

Antimicrobial peptide HPA3NT3-A2 effectively inhibits biofilm formation in mice infected with drug-resistant bacteria

2019 ◽  
Vol 7 (12) ◽  
pp. 5068-5083 ◽  
Author(s):  
Jong-Kook Lee ◽  
Loredana Mereuta ◽  
Tudor Luchian ◽  
Yoonkyung Park
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Peptide ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Resistant Bacteria ◽  
Bacterial Biofilms ◽  
Drug Resistant ◽  
Drug Resistant Bacteria ◽  
Serious Infections

Bacterial biofilms formed through secretion of extracellular polymeric substances (EPS) have been implicated in many serious infections and can increase antibiotic resistance by a factor of more than 1000.

Antimicrobial activity of essential oil ofArtemisia judaicaL. from Algeria against multi-drug resistant bacteria from clinical origin

2015 ◽  
Vol 31 (2) ◽  
pp. 137-142 ◽  
Author(s):  
N. Benmansour ◽  
A. Benmansour ◽  
F. El Hanbali ◽  
M. C. González-Mas ◽  
M. A. Blázquez ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Drug Resistant Bacteria

scholarly journals Antimicrobial activity of Guava tree (Psidium Guajava) leaf extract and selected Commercial Antibiotics on Bacterial isolates from Kisubi Hospital

2020 ◽  
Author(s):  
◽  
David Serunjogi ◽  
Kizito Muwonge
Keyword(s):  
Antimicrobial Activity ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Leaf Extracts ◽  
Drug Resistant Bacteria ◽  
The World ◽  
Guava Leaf ◽  
Tree Leaf

Background: Plant species such as the guava have been used in Uganda and elsewhere in the world to treat some of the medical conditions associated with bacteria, this is due to the increased number of drug resistant bacteria in the world. In this study, the antimicrobial activity of guava leaf extracts against some of the disease causing bacteria isolated from Kisubi hospital in Uganda is discussed. Methods: The guava leafs were collected from a plantation near Zika forest in Uganda (0°7′27″N 32°31′32″E / 0.12417°N 32.52556°E / 0.12417; 32.52556) and samples were put in a bag and transferred to the university laboratory where they were identified. The extracts were obtained by maceration using distilled water, 30%, 50% and 70 % methanol as the extraction solvents. Antimicrobial susceptibility testing was conducted using the disc diffusion method. Results: Gram-negative Escherichia coli was sensitive to the plant extract and synthetic commercial drugs such as trimethoprim-sulfamethozole, ciprofloxacin, and Gentamicin. Pseudomonas aeruginosa was resistant to all drugs. Streptococcus pneumonia and Staphylococcus aureus were all sensitive to the plant extracts with measurable inhibition zones. Conclusion: The Guava tree leaf crude extracts have antimicrobial activity against drug-resistant bacteria. More studies should be carried out to know the potency and the concentration of different plant origin extracts.

scholarly journals Potential of Novel Antimicrobial Peptide P3 from Bovine Erythrocytes and Its Analogs To Disrupt Bacterial MembranesIn Vitroand Display Activity against Drug-Resistant Bacteria in a Mouse Model

2015 ◽  
Vol 59 (5) ◽  
pp. 2835-2841 ◽  
Author(s):  
Qinghua Zhang ◽  
Yanzhao Xu ◽  
Qing Wang ◽  
Bolin Hang ◽  
Yawei Sun ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Body Weight ◽  
Mouse Model ◽  
Antimicrobial Activities ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Drug Resistant Bacteria ◽  
Resistant Strains ◽  
Bovine Erythrocytes

ABSTRACTWith the emergence of many antibiotic-resistant strains worldwide, antimicrobial peptides (AMPs) are being evaluated as promising alternatives to conventional antibiotics. P3, a novel hemoglobin peptide derived from bovine erythrocytes, exhibited modest antimicrobial activityin vitro. We evaluated the antimicrobial activities of P3 and an analog, JH-3, bothin vitroandin vivo. The MICs of P3 and JH-3 ranged from 3.125 μg/ml to 50 μg/ml when a wide spectrum of bacteria was tested, including multidrug-resistant strains. P3 killed bacteria within 30 min by disrupting the bacterial cytoplasmic membrane and disturbing the intracellular calcium balance. Circular dichroism (CD) spectrometry showed that P3 assumed an α-helical conformation in bacterial lipid membranes, which was indispensable for antimicrobial activity. Importantly, the 50% lethal dose (LD50) of JH-3 was 180 mg/kg of mouse body weight after intraperitoneal (i.p.) injection, and no death was observed at any dose up to 240 mg/kg body weight following subcutaneous (s.c.) injection. Furthermore, JH-3 significantly decreased the bacterial count and rescued infected mice in a model of mouse bacteremia. In conclusion, P3 and an analog exhibited potent antimicrobial activities and relatively low toxicities in a mouse model, indicating that they may be useful for treating infections caused by drug-resistant bacteria.

scholarly journals Synthesis and preliminary anti-inflammatory and anti-bacterial evaluation of some diflunisal aza-analogs

MedChemComm ◽  
2018 ◽  
Vol 9 (6) ◽  
pp. 1017-1032
Author(s):  
Davide Carta ◽  
Paola Brun ◽  
Matteo Dal Pra ◽  
Giulia Bernabè ◽  
Ignazio Castagliuolo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Inflammatory Activity ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Drug Resistant Bacteria ◽  
Anti Inflammatory

The new diflunisal aza-analogs multi-target approach is remarkable in the treatment of infections induced by multi-drug resistant bacteria. Diflunisal aza-analogs preserved the anti-inflammatory activity and significantly potentiated the antimicrobial activity of antibiotics.

Sign in / Sign up

Export Citation Format

Share Document